Novel theranostic zinc phthalocyanine–phospholipid complex self-assembled nanoparticles for imaging-guided targeted photodynamic treatment with controllable ROS production and shape-assisted enhanced cellular uptake

2018 ◽  
Vol 162 ◽  
pp. 76-89 ◽  
Author(s):  
Jinyuan Ma ◽  
Yang Li ◽  
Guihua Liu ◽  
Ai Li ◽  
Yilin Chen ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Zinc Phthalocyanine ◽  
Ros Production ◽  
Phospholipid Complex ◽  
Photodynamic Treatment ◽  
Self Assembled

scholarly journals In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1906
Author(s):  
Mona Atabakhshi-Kashi ◽  
Mónica Carril ◽  
Hossein Mahdavi ◽  
Wolfgang J. Parak ◽  
Carolina Carrillo-Carrion ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cellular Uptake ◽  
Hydrophobic Interactions ◽  
Intrinsic Properties ◽  
Wide Range ◽  
Self Assembled ◽  
Uptake Studies ◽  
Targeting Ability ◽  
Fluorinated Nanoparticles

Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine–fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly).

scholarly journals Ultrastructural Aspects of Photodynamic Inactivation of Highly Pathogenic Avian H5N8 Influenza Virus

Viruses ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 955 ◽  
Author(s):  
Korneev ◽  
Kurskaya ◽  
Sharshov ◽  
Eastwood ◽  
Strakhovskaya
Keyword(s):  
Influenza Virus ◽  
Lipid Membranes ◽  
Structural Integrity ◽  
Mdck Cells ◽  
Virus Titer ◽  
Surface Protein ◽  
Zinc Phthalocyanine ◽  
Photodynamic Inactivation ◽  
Photodynamic Treatment ◽  
Ultrastructural Studies

Ultrastructural studies revealing morphological differences between intact and photodynamically inactivated virions can point to inactivation mechanisms and molecular targets. Using influenza as a model system, we show that photodynamic virus inactivation is possible without total virion destruction. Indeed, irradiation with a relatively low concentration of the photosensitizer (octacationic octakis(cholinyl) zinc phthalocyanine) inactivated viral particles (the virus titer was determined in Madin Darby Canine Kidney (MDCK) cells) but did not destroy them. Transmission electron microscopy (TEM) revealed that virion membranes kept structural integrity but lost their surface glycoproteins. Such structures are known as “bald” virions, which were first described as a result of protease treatment. At a higher photosensitizer concentration, the lipid membranes were also destroyed. Therefore, photodynamic inactivation of influenza virus initially results from surface protein removal, followed by complete virion destruction. This study suggests that photodynamic treatment can be used to manufacture “bald” virions for experimental purposes. Photodynamic inactivation is based on the production of reactive oxygen species which attack and destroy biomolecules. Thus, the results of this study can potentially apply to other enveloped viruses and sources of singlet oxygen.

Self-assembled micelles based on gambogenic acid-phospholipid complex for sustained-release drug delivery

2019 ◽  
pp. 1-10
Author(s):  
Beilei Wang ◽  
Weiye Cheng ◽  
Caiyun Zhang ◽  
Youmei Bao ◽  
Liqiong Zha ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Sustained Release ◽  
Phospholipid Complex ◽  
Release Drug ◽  
Self Assembled

Enhanced Cellular Uptake and Cytotoxicity of Doxorubicin by Self-Assembled Lactobionate-Phytosterol-Alginate Nanoparticles

2017 ◽  
Vol 17 (7) ◽  
pp. 4558-4566
Author(s):  
Xue Wang ◽  
Meiling Huang ◽  
Liangping Li ◽  
Mingming Song ◽  
Renmin Gong
Keyword(s):  
Cellular Uptake ◽  
Self Assembled

scholarly journals Shear Stress-Dependent Targeting Efficiency Using Self-Assembled Gelatin–Oleic Nanoparticles in a Biomimetic Microfluidic System

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 555
Author(s):  
Taehee Kang ◽  
Chulhun Park ◽  
Nileshkumar Meghani ◽  
Thao T.D. Tran ◽  
Phuong H.L. Tran ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Flow ◽  
Cellular Uptake ◽  
Static Condition ◽  
Microfluidic System ◽  
Water Soluble ◽  
Cellular Behaviors ◽  
Self Assembled ◽  
Stress Dependent ◽  
Coumarin 6

Cellular properties and microenvironments, as well as the characteristics of nanoparticles (NPs), affect the cellular uptake and cytotoxic effects of drug-loaded NPs. Since there is fluid flow in the human blood system, fluid flow also affects the drug delivery efficiency of NPs. This study aimed to evaluate the cellular behaviors of drug-loaded soft NPs on A549 cancer cells under different levels of shear stress (0.5, 5, and 50 dynes/cm2) in the biomimetic microfluidic system. The soft self-assembled NPs were formed by the gelatin–oleic conjugate (GOC). The poorly water-soluble coumarin-6 or paclitaxel (PTX) were used as model markers for encapsulation within self-assembled NPs (C-GONs or PTX-GONs, respectively). The cellular uptake of C-GONs was found to be improved with shear-stress dependence. The inhibitory concentration (IC50) of PTX-GONs at 0.5, 5, and 50 dynes/cm2 was 0.106 µg/mL, 0.108 µg/mL, and 0.091 µg/mL, respectively, as compared to 0.138 µg/mL in a static condition. The cell killing efficiency of PTX-GONs was increased in the highest shear stress of 50 dynes/cm2 in the static condition, and other levels of shear stress in dynamic conditions.

Sign in / Sign up

Export Citation Format

Share Document