Cationic poly(cyclodextrin)/alginate nanocapsules: From design to application as efficient delivery vehicle of 4-hydroxy tamoxifen to podocyte in vitro

Tumor targeting has emerged as an advantageous approach to improving the efficacy and safety of cytotoxic agents or radiolabeled ligands that do not preferentially accumulate in the tumor tissue. The somatostatin receptors (SSTRs) belong to the G-protein-coupled receptor superfamily and they are overexpressed in many neuroendocrine tumors (NETs). SSTRs can be efficiently targeted with octreotide, a cyclic octapeptide that is derived from native somatostatin. The conjugation of cargoes to octreotide represents an attractive approach for effective tumor targeting. In this study, we conjugated octreotide to cryptophycin, which is a highly cytotoxic depsipeptide, through the protease cleavable Val-Cit dipeptide linker using two different self-immolative moieties. The biological activity was investigated in vitro and the self-immolative part largely influenced the stability of the conjugates. Replacement of cryptophycin by the infrared cyanine dye Cy5.5 was exploited to elucidate the tumor targeting properties of the conjugates in vitro and in vivo. The compound efficiently and selectively internalized in cells overexpressing SSTR2 and accumulated in xenografts for a prolonged time. Our results on the in vivo properties indicate that octreotide may serve as an efficient delivery vehicle for tumor targeting.

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Combination of Microemulsion for Topical Deliverying Tripterygium Wilfordii Multiglycoside

Materials Science Forum ◽

10.4028/www.scientific.net/msf.694.881 ◽

2011 ◽

Vol 694 ◽

pp. 881-885

Author(s):

Xin He ◽

Xing Hua Zhao ◽

Wu Qing Ou Yang

Keyword(s):

Immune Suppression ◽

Topical Delivery ◽

Skin Permeability ◽

Tripterygium Wilfordii ◽

Delivery Vehicle ◽

Control Solution ◽

Suppression Effect ◽

Low Viscosity ◽

Spherical Droplets

The purpose of this study was to explore the potential application of the combination of microemulsion as a topical delivery vehicle in enhancing the absorption and efficacy of tripterygium wilfordii multiglycoside (TWM). Various microemulsion formulations were developed and an optimal microemulsion (TWM-ME)，which presented spherical droplets and consisted of RH-40, IPM and water was 27: 3.3: 69.7 by weight. It possessd an average droplet size of 23.6 nm, a low viscosity of (3.56±0.12) mm2•s-1 Zeta electric potential was (–5.35±0.42) mV, refractive index was (1.3617±0.0051) nD20, conductivity was (97.6±3.6) μs/cm. Compared to the control solution, TWM-ME provided better skin permeability in vitro. Moreover, TWM-ME has noticeable anti-inflammatory and immune suppression effect. These results indicate that the combination of microemulsion represents an effective vehicle for topical delivery of TWM.

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Bombesin-Tethered Reactive Oxygen Species (ROS)-Responsive Nanoparticles for Monomethyl Auristatin F (MMAF) Delivery

Bioengineering ◽

10.3390/bioengineering8040043 ◽

2021 ◽

Vol 8 (4) ◽

pp. 43

Author(s):

Jihoon Kim ◽

Jee Seon Kim ◽

Kyung Hyun Min ◽

Young-Hwa Kim ◽

Xiaoyuan Chen

Keyword(s):

Blood Circulation ◽

Burst Release ◽

Oxygen Species ◽

Antibody Drug Conjugate ◽

Gastrin Releasing Peptide ◽

Anticancer Effects ◽

Efficient Delivery ◽

Nanoparticle System ◽

Micelle System

Dolastatin derivatives, represented by monomethylauristatin E (MMAE), have been translated in clinic with a form of antibody–drug conjugate; however, their potential in nanoparticle systems has not been well established due to the potential risk of immature release of extremely high cytotoxic dolastatin drugs during blood circulation. Herein, we rationally propose monomethylauristatin F (MMAF), a dolastatin-derived, loaded nanoparticle system composed of bombesin (BBN)-tethered ROS-responsive micelle system (BBN-PEG-PPADT) to achieve efficient anticancer therapy with targeted and efficient delivery of MMAF. The developed MMAF-loaded BBN-PEG-PPADT micelles (MMAF@BBN-PEG-PPADT) exhibited improved cellular uptake via interactions between BBN and gastrin-releasing peptide receptors on the cancer cells and the intracellular burst release of MMAF, owing to the ROS-responsive disruption, which allowed the efficient anticancer effects of MMAF in vitro. This study suggests the potential of nanoparticle systems in the delivery of dolastatin drugs.

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Chapter 12: Hollow Chitosan‒Alginate Multilayer Microcapsules as Drug Delivery Vehicle: Doxorubicin Loading and in vitro and in vivo Studies

Nanomedicine in Cancer ◽

10.1201/9781315114361-13 ◽

2017 ◽

pp. 317-346

Keyword(s):

Drug Delivery ◽

In Vivo Studies ◽

Delivery Vehicle ◽

Drug Delivery Vehicle

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Olive Oil Based Organogels for Effective Topical Delivery of Fluconazole: In-vitro Antifungal Study

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2020/v32i2530821 ◽

2020 ◽

pp. 29-36

Author(s):

Mohammad Muqtader Ahmed ◽

Farhat Fatima ◽

Abdul Bari Mohammed

Keyword(s):

Olive Oil ◽

Ex Vivo ◽

Similarity Index ◽

Ex Vivo Permeation ◽

Efficient Delivery ◽

In Vitro Diffusion ◽

Permeation Studies ◽

The Stability ◽

Hot Melt

The objective of the study was to formulate olive oil based organogels for the topical application of fluconazole (FLZ), to ensure the efficient delivery of the drug deeper in to the skin layers. Methods: Nine formulations developed by hot-melt method using olive oil, sorbitan monostearate (SMS) and FLZ. Prepared formulations characterized for macro evaluations, pH, spreadibility, viscosity, gel-sol transition, in-vitro diffusion study. Further optimized formulation evaluated for ex-vivo percutaneous permeation, in-vitro antifungal studies and stability studies by similarity index. Results: The results of evaluated parameters ensure the stability and effectiveness of the prepared olive oil based organogels. In-vitro diffusion studied reflects decrease in drug release with increase in surfactant concentration due to increase in viscosity. Moreover, ex-vivo permeation studies revealed that the permeation of FLZ was enhanced for optimized formulations (F6) as compared to the marketed gel formulation. Further, the optimized formulation exhibits the broad zone of inhibition against fungal strains in comparison to control and marketed product during in-vitro antifungal study. Conclusion: The olive oil based organogels formulation shown the enhanced permeation of FLZ from organogel network structure with good antifungal activity as compared to the marketed formulation. Henceforth, the FLZ organogel formulations could be used topically for the effective treatment of fungal infection.

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In vitro and in vivo evaluation of folate-mediated PEGylated nanostructured lipid carriers for the efficient delivery of furanodiene

Drug Development and Industrial Pharmacy ◽

10.1080/03639045.2017.1328429 ◽

2017 ◽

Vol 43 (10) ◽

pp. 1610-1618 ◽

Cited By ~ 6

Author(s):

Jianmei Zhang ◽

Yunpeng He ◽

Jianqi Jiang ◽

Meng Li ◽

Chenhao Jin ◽

...

Keyword(s):

Nanostructured Lipid Carriers ◽

In Vivo Evaluation ◽

Efficient Delivery

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Bovine Serum Albumin Nanoparticles for the Efficient Delivery of Berberine: Preparation, Characterization and In vitro biological studies

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2020.125501 ◽

2021 ◽

Vol 608 ◽

pp. 125501

Author(s):

Raghu Solanki ◽

Krunal Patel ◽

Sunita Patel

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Albumin Nanoparticles ◽

Efficient Delivery ◽

Biological Studies

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Bioreducible poly(urethane amine)s for robust nucleic acid transfection in stem cells

Biomaterials Science ◽

10.1039/c9bm00605b ◽

2019 ◽

Vol 7 (8) ◽

pp. 3510-3518

Author(s):

Ying Ye ◽

Rong Jin ◽

Xiaoxin Hu ◽

Juhua Zhuang ◽

Wei Xia ◽

...

Keyword(s):

Stem Cells ◽

Embryonic Stem Cells ◽

Nucleic Acid ◽

Embryonic Stem ◽

Highly Efficient ◽

Efficient Delivery ◽

Low Cytotoxicity

Bioreducible poly(urethane amine)s can be designed and optimized for highly efficient delivery in vitro of DNA or SiRNA into adult or embryonic stem cells with low cytotoxicity.

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