Leishmania major strains isolated from distinct endemic areas show diverse cytokine mRNA expression levels in C57BL/6 mice: Toward selecting an ideal strain for the vaccine studies

Cytokine ◽  
2015 ◽  
Vol 76 (2) ◽  
pp. 303-308 ◽  
Author(s):  
Sima Darabi ◽  
Vahid Khaze ◽  
Farhad Riazi-Rad ◽  
Haiedeh Darabi ◽  
Fariborz Bahrami ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Leishmania Major ◽  
Cytokine Mrna ◽  
Expression Levels ◽  
Cytokine Mrna Expression ◽  
Endemic Areas ◽  
Mrna Expression Levels

ANATOMIC SITE AND IMMUNE FUNCTION CORRELATE WITH RELATIVE CYTOKINE mRNA EXPRESSION LEVELS IN LYMPHOID TISSUES OF NORMAL RHESUS MACAQUES

Cytokine ◽  
2001 ◽  
Vol 16 (5) ◽  
pp. 191-204 ◽  
Author(s):  
Kristina Abel ◽  
Michelle J. Alegria-Hartman ◽  
Kenneth Zanotto ◽  
Michael B. McChesney ◽  
Marta L. Marthas ◽  
...  
Keyword(s):  
Immune Function ◽  
Mrna Expression ◽  
Rhesus Macaques ◽  
Lymphoid Tissues ◽  
Cytokine Mrna ◽  
Anatomic Site ◽  
Expression Levels ◽  
Cytokine Mrna Expression ◽  
Mrna Expression Levels

scholarly journals Comparative Analysis of Cytokine mRNA Expressions in Human Tissues with Mycobacterium tuberculosis Infection

2021 ◽  
Author(s):  
Sung-Bae Park ◽  
Heechul Park ◽  
Yoon-Sung Choi ◽  
Ji Young Park ◽  
Dongsup Lee ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mrna Expression ◽  
The Body ◽  
Molecular Diagnostic ◽  
Human Tissues ◽  
Immune Markers ◽  
Cytokine Mrna ◽  
Expression Levels ◽  
Gm Csf ◽  
Mrna Expression Levels

In the present study, we aimed to investigate whether an automated molecular diagnostic method based on PCR-reverse blot hybridization assay can discriminate between human Mycobacterium tuberculosis (MTB)-positive and -negative FFPE tissues and to compare the relative mRNA expression levels of various host immune markers between MTB-infected and uninfected human tissues using quantitative reverse transcription (qRT) PCR. A total of 52 human FFPE tissue samples from various regions of the body, including the lungs, lymph nodes, tendons, colon, and appendix, were collected and used for the molecular identification of Mycobacterium species and analysis of cytokine mRNA expression. As a result, IFN-γ, TNF-α, IP-10, CXCL9, CXCL11, and GM-CSF mRNA expression levels in MTB-infected tissues were significantly higher than those in uninfected samples. Additionally, the differences in the mRNA expression levels of IFN-γ, CXCL9, and GM-CSF between MTB-infected and uninfected tissues were statistically significant were statistically significant (p < 0.05). Correlation curve analysis indicated that the mRNA expression of IFN-γ was inversely proportional to that of IP-10 and that the mRNA expression levels of IFN-γ, TNF-α, CXCL9, CXCL11, GM-CSF, and TNFR were proportional and well-correlated. Furthermore, to establish marker profiles for detecting MTB infection, the statistically significant expression levels of three markers were combined. We confirmed that the combined profile of IFN-γ, CXCL9, and GM-CSF expression levels was statistically significant (P < 0.001). Although the mRNA expression patterns of host immune markers may vary according to MTB infection status, these patterns may be highly correlated and can be simultaneously used as an additional indicator for diagnosing TB.

CYTOKINE mRNA EXPRESSION IN CHICKEN EXPERIMENTALLY INFECTED WITH DIFFERENT AVIAN REOVIRUS STRAINS

2014 ◽  
Vol 40 (01) ◽  
pp. 29-36 ◽  
Author(s):  
Pin-Chun Shen ◽  
Jia-Ling Yang ◽  
Bor-Sheu Su ◽  
Long-Huw Lee
Keyword(s):  
Mrna Expression ◽  
Post Inoculation ◽  
Avian Reovirus ◽  
Cytokine Mrna ◽  
Expression Levels ◽  
Real Time Quantitative Pcr ◽  
Cytokine Mrna Expression ◽  
Gross Lesions ◽  
Ifn Γ ◽  
Foot Pad

This study was undertaken to elucidate the cytokine response in chicken infected with strains of avian reovirus (ARV) S1133 and 2408. The expression levels of cytokine mRNA in the spleen and viral S1 RNA in various tissues at 1.5 and 2.5 days post inoculation (dpi) were examined using real-time quantitative PCR. Among the cytokines examined, the mRNA expression levels of IL-6, IFN-γ, IL-10 and iNOS at 2.5 dpi were significantly upregulated and higher in chickens infected with strain 2408 than in chickens infected with strain S1133, particularly IL-6 and IFN-γ. A significantly higher levels of viral S1 RNA were detected in the examined tissues from chickens infected with strain 2408 than with strain S1133 over the experimental course, among which the foot pad and spleen were more predominant. The highest levels of IL-6 and IFN-γ mRNA expression correlated with the viral S1 RNA levels in the spleen and the marked clinical diseases and gross lesions, suggesting that IL-6 and IFN-γ may play a role in the pathogenesis of ARV infection.

scholarly journals The role of CXCR3 and its ligands expression in Brucellar spondylitis

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Xin Hu ◽  
Xiaoqian Shang ◽  
Liang Wang ◽  
Jiahui Fan ◽  
Yue Wang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
Mononuclear Cells ◽  
Healthy Controls ◽  
Inflammatory Infiltration ◽  
Peripheral Blood Mononuclear ◽  
Expression Levels ◽  
Inflammatory Damage ◽  
Ifn Γ ◽  
Mrna Expression Levels

Abstract Aim Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS. Methods A total of 29 BS patients and 15 healthy controls were enrolled. Real-Time PCR was used to detect the mRNA expression levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood mononuclear cells (PBMCs) of BS patients and healthy controls. Hematoxylin-Eosin staining was used to show the pathological changes in BS lesion tissues. Immunohistochemistry staining was used to show the protein expression levels of Brucella-Ab, IFN-γ, CXCR3, CXCL9 and CXCL10 in BS lesion tissues. At the same time, ELISA was used to detect the serum levels of IFN-γ, CXCL9 CXCL10 and autoantibodies against CXCR3 in patients with BS. Results In lesion tissue of BS patients, it showed necrosis of cartilage, acute or chronic inflammatory infiltration. Brucella-Ab protein was abundantly expressed in close lesion tissue. And the protein expression levels of IFN-γ, CXCR3 and CXCL10 were highly expressed in close lesion tissue and serum of BS patients. At the same time, the mRNA expression levels of IFN-γ, CXCR3 and CXCL10 in PBMCs of BS patients were significantly higher than those in controls. Conclusion In our research, the expression levels of IFN-γ, CXCR3 and its ligands were significantly higher than those in controls. It suggested that high expression levels of IFN-γ, CXCR3 and its ligands indicated a serious inflammatory damage in patients with BS.

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