Elaboration of multimodal ligands based on BOX units: toward full zinc release in Zn(II) complexes by external stimulation

2021 ◽  
pp. 109476
Author(s):  
Youssef Aidibi ◽  
Magali Allain ◽  
Abdelkrim El-Ghayoury ◽  
Philippe Leriche ◽  
Lionel Sanguinet
Keyword(s):  
External Stimulation ◽  
Zinc Release

STUDY OF CXCL12, CCR4, EGFR GENE EXPRESSION IN MIGRATING MYELOMONOBLASTIC LEUKEMIA CELLS BEFORE AND AFTER CHEMOTHERAPY

2019 ◽  
Vol 1 (1) ◽  
pp. 100-104
Author(s):  
A. B. Filina ◽  
O. A. Svitich ◽  
Yu. I. Ammur ◽  
A. K. Golenkov ◽  
E. F. Klinushkina ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Mononuclear Cells ◽  
Boyden Chamber ◽  
External Stimulation ◽  
Healthy Donors ◽  
Genes Expression ◽  
Before And After ◽  
Chemokine Cxcl12 ◽  
And Migration ◽  
Cell Chemotaxis

Аim. A study of CXCL12 effect on the migration of mononuclear cells isolated from healthy patients, from patients with myelomonoblastic leukemia before and after chemotherapy and the study of CCR4, EGFR and CXCL12 genes expression after exposure to CXCL12. Materials and methods. The chemotaxis of mononuclear cells (MNCs) of healthy donors and patients with myelomonoblastic leukemia was studied in a Boyden chamber, followed by isolation of RNA, reverse transcription and PCR-RV. Results. A significant increase in myelomonoblasic cell chemotaxis towards CXCL12 after chemotherapy was demonstrated, as well as a decrease in the expression of this chemokine in tumor cells before chemotherapy after exposure to CXCL12. Сonclusion. Presumably, the tumor cells themselves produce CXCL12 in large amounts, which is necessary for the disturbance of intercellular interactions and further intravasation, whose production may decrease with external stimulation by the same chemokine. CXCL12 also helps to increase the expression level of EGFR and CCR4, which leads to increased tumor proliferation and migration of tumor cells.

scholarly journals Secretome screening reveals immunomodulating functions of IFNα-7, PAP and GDF-7 on regulatory T-cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mei Ding ◽  
Rajneesh Malhotra ◽  
Tomas Ottosson ◽  
Magnus Lundqvist ◽  
Aman Mebrahtu ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Cell Function ◽  
Foxp3 Expression ◽  
Treg Cells ◽  
Secreted Proteins ◽  
External Stimulation ◽  
Marker Proteins ◽  
Positive Regulators ◽  
Conformational Model

AbstractRegulatory T cells (Tregs) are the key cells regulating peripheral autoreactive T lymphocytes. Tregs exert their function by suppressing effector T cells. Tregs have been shown to play essential roles in the control of a variety of physiological and pathological immune responses. However, Tregs are unstable and can lose the expression of FOXP3 and suppressive functions as a consequence of outer stimuli. Available literature suggests that secreted proteins regulate Treg functional states, such as differentiation, proliferation and suppressive function. Identification of secreted proteins that affect Treg cell function are highly interesting for both therapeutic and diagnostic purposes in either hyperactive or immunosuppressed populations. Here, we report a phenotypic screening of a human secretome library in human Treg cells utilising a high throughput flow cytometry technology. Screening a library of 575 secreted proteins allowed us to identify proteins stabilising or destabilising the Treg phenotype as suggested by changes in expression of Treg marker proteins FOXP3 and/or CTLA4. Four proteins including GDF-7, IL-10, PAP and IFNα-7 were identified as positive regulators that increased FOXP3 and/or CTLA4 expression. PAP is a phosphatase. A catalytic-dead version of the protein did not induce an increase in FOXP3 expression. Ten interferon proteins were identified as negative regulators that reduced the expression of both CTLA4 and FOXP3, without affecting cell viability. A transcriptomics analysis supported the differential effect on Tregs of IFNα-7 versus other IFNα proteins, indicating differences in JAK/STAT signaling. A conformational model experiment confirmed a tenfold reduction in IFNAR-mediated ISG transcription for IFNα-7 compared to IFNα-10. This further strengthened the theory of a shift in downstream messaging upon external stimulation. As a summary, we have identified four positive regulators of FOXP3 and/or CTLA4 expression. Further exploration of these Treg modulators and their method of action has the potential to aid the discovery of novel therapies for both autoimmune and infectious diseases as well as for cancer.

Human Brain Like Memory Behavior in the Magnetic Domain Expansion Type Magneto-Optical Disk

2001 ◽  
Vol 674 ◽  
Author(s):  
Norio Ota ◽  
Hiroyuki Awano ◽  
Manabu Tani ◽  
Susumu Imai
Keyword(s):  
Magnetic Field ◽  
Human Brain ◽  
Magnetic Domain ◽  
Field Pattern ◽  
Human Response ◽  
External Stimulation ◽  
Stored Information ◽  
The Magnetic Field ◽  
Timing Phase ◽  
Coherent Amplification

ABSTRACTMagnetic Amplifying Magneto-Optical System (MAMMOS) shows human brain like memory behavior. Magnetic field and laser power have threshold to recover the stored memory like the human response of remembering. MAMMOS also has a feature to amplify very small recorded signals like our recovery of memory, e.g. fifty years ago episode.By adding the meaningful information on the magnetic field pattern, we can get some correlation between our memory and external stimulation. Such scheme is named as “the Active readout MAMMOS” which is analogues to the human process of remembering the memory.If the applied field pattern and timing phase just coincide with stored information, there occurs the coherent amplification of MAMMOS signal. We can utilize such phenomena as the trigger of “Memory Association”.

Zinc release from Schaffer collaterals and its significance

2006 ◽  
Vol 68 (6) ◽  
pp. 442-447 ◽  
Author(s):  
Atsushi Takeda ◽  
Satoko Nakajima ◽  
Sayuri Fuke ◽  
Naomi Sakurada ◽  
Akira Minami ◽  
...  
Keyword(s):  
Schaffer Collaterals ◽  
Zinc Release

On the Hypothesized Physiological Correspondence between Perceptual and Imagery Processes

1991 ◽  
Vol 73 (3) ◽  
pp. 827-830 ◽  
Author(s):  
Vezio Ruggieri
Keyword(s):  
Undergraduate Students ◽  
Mental Image ◽  
Female Undergraduate ◽  
External Stimulation ◽  
Stimulation Of

This research examined some aspects of the interaction between imagery and perception of 16 male and 84 female undergraduate students in psychology. The data indicate that, when a subject is imagining with open eyes, a modification in the external stimulation of the retina, produced by covering the eyes, strongly modifies the imaginative activity. The majority of the subjects observed a loss of mental image. In 54% of the subjects the loss of image was provoked by covering only one of the two eyes. Other subjects presented interesting alterations of the mental image. The observed differences among subjects are interpreted by hypothesizing a correspondence between perceptual and imaginative styles that could employ common neurophysiological structures at central and perhaps peripheral levels.

scholarly journals Different Brain Network Activations Induced by Modulation and Nonmodulation Laser Acupuncture

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Chang-Wei Hsieh ◽  
Jih-Huah Wu ◽  
Chao-Hsien Hsieh ◽  
Qwa-Fun Wang ◽  
Jyh-Horng Chen
Keyword(s):  
Primary Motor Cortex ◽  
Parietal Lobe ◽  
Random Effect ◽  
Sensory Information ◽  
Brain Network ◽  
Laser Acupuncture ◽  
Middle Temporal Gyrus ◽  
Left Frontal Lobe ◽  
External Stimulation ◽  
Acupuncture Stimulation

The aim of this study is to compare the distinct cerebral activation with continued wave (CW) and 10 Hz-modulated wave (MW) stimulation during low-level laser acupuncture. Functional magnetic resonance imaging (fMRI) studies were performed to investigate the possible mechanism during laser acupuncture stimulation at the left foot's yongquan (K1) acupoint. There are 12 healthy right-handed volunteers for each type of laser stimulation (10-Hz-Modulated wave: 8 males and 4 females; continued wave: 9 males and 3 females). The analysis of multisubjects in this experiment was applied by random-effect (RFX) analysis. In CW groups, significant activations were found within the inferior parietal lobule, the primary somatosensory cortex, and the precuneus of left parietal lobe. Medial and superior frontal gyrus of left frontal lobe were also aroused. In MW groups, significant activations were found within the primary motor cortex and middle temporal gyrus of left hemisphere and bilateral cuneus. Placebo stimulation did not show any activation. Most activation areas were involved in the functions of memory, attention, and self-consciousness. The results showed the cerebral hemodynamic responses of two laser acupuncture stimulation modes and implied that its mechanism was not only based upon afferent sensory information processing, but that it also had the hemodynamic property altered during external stimulation.

Cardiac Ganglion of Limulus: Intracellular Activity in the Unipolar Cells

1970 ◽  
Vol 53 (2) ◽  
pp. 411-423
Author(s):  
VINCENT J. PALESE ◽  
JANE L. BECKER ◽  
RALPH A. PAX
Keyword(s):  
Functional Relationship ◽  
Membrane Resistance ◽  
Recovery Phase ◽  
Resting Membrane Potential ◽  
Cardiac Ganglion ◽  
External Stimulation ◽  
A Value ◽  
Number Of Factors ◽  
Soma Membrane ◽  
Specific Membrane

1. The electrical activity of unipolar cells of the cardiac ganglion of Limulus has been examined with microelectrodes to determine the electrical properties and the functional relationship between unipolar cells. 2. Resting membrane potential in these cell averages -43 mV. 3. Correlated with each contraction of the heart there occurs a burst of activity which consists of a sustained depolarization of several seconds on which is superimposed a series of small 2-5 mV spikes. 4. Activity which occurs during the initial phase appears to be the result of pre-synaptic inputs to these cells. Spike activity during the recovery phase is probably endogenous to the cell from which we are recording. 5. The specific membrane resistance for the soma membrane averages 12700Ω.cm2. The time constant has a value of 19.6ms. The specific membrane capacitance measures endogenous to the cell from which we are recording. 6. External stimulation produces a number of different responses in the unipolar cells which are dependent upon a number of factors. These responses can be classified into at least three types: (a) a driven response similar to a spontaneous burst; (b) slowly rising potentials which may be antidromic spikes.

Changes in regulation of human zinc metabolism with age

1992 ◽  
Vol 263 (5) ◽  
pp. R1162-R1168 ◽  
Author(s):  
M. E. Wastney ◽  
S. Ahmed ◽  
R. I. Henkin
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Zinc Concentration ◽  
Kinetic Studies ◽  
Compartmental Analysis ◽  
Whole Body ◽  
Zinc Metabolism ◽  
Zinc Release ◽  
Urinary Zinc Excretion ◽  
Using Data

To assess changes in zinc metabolism with age, kinetic studies were performed in healthy adults (26 men, 21 women) aged 20-84 yr after a single oral or intravenous bolus of 65Zn. Studies covered two consecutive 9-mo periods while subjects were on a basal dietary intake of approximately 10 mg Zn/day and while taking an additional 100 mg Zn/day orally. Zinc metabolism was analyzed by compartmental analysis using data from plasma, red blood cells, urine, feces, liver, thigh, and whole body [M. E. Wastney, R. L. Aamodt, W. F. Rumble, and R. I. Henkin. Am. J. Physiol. 251 (Regulatory Integrative Comp. Physiol. 20): R398-R408, 1986]. Changes in observed and model calculated values of zinc metabolism were assessed on age by regression. During basal state, zinc release from red blood cells decreased with age. During zinc loading, response (defined as change from basal state) of plasma zinc concentration, urinary zinc excretion, and liver zinc increased with age, while response of fraction of zinc taken up by red blood cells decreased with age. In men, response of amount of zinc absorbed increased with age and in women response of fraction of endogenous zinc excreted decreased with age. Four responses that changed with age (urinary excretion, red blood cell exchange, absorption, and endogenous excretion) occurred at previously defined sites of regulation of zinc metabolism. Results show that regulation of zinc metabolism changes with age.

The individualism of motivation

2019 ◽  
Vol 18 (3) ◽  
pp. 96-103
Author(s):  
Andrew Mayo
Keyword(s):  
Employee Engagement ◽  
Psychological Contract ◽  
Extrinsic Motivation ◽  
Well Being ◽  
Human Condition ◽  
Goal Theory ◽  
Content Type ◽  
External Stimulation ◽  
Writing Models ◽  
Practical Implications

Purpose The purpose of this paper is to examine the components that drive employee engagement and show how important it is to understand the individualism of motivation as against generic assumptions. Design/methodology/approach This paper defines employee engagement and takes four drivers that influence it in turn. These are analysed and discussed, particularly as to the individualistic elements of them. Two approaches to understanding individual goals and priorities are illustrated. Findings There are four drivers of engagement, namely, the absence of dissatisfaction or irritation factors, intrinsic motivation (inner driven) and extrinsic motivation (external stimulation) and personal well-being. Each of these have highly individualistic elements, and models based on a generic human condition do not work effectively. Two instruments for understanding this individualism are illustrated, one based on goal theory and another based on the psychological contract. Practical implications Many motivational efforts fail because of an assumed commonality in what motivates people. The reality is that different personalities and different personal goals and values require individual approaches. Successful engagement demands that leader/managers make it a priority to understand each one of their people in these terms. Originality/value This paper is based mostly on the writing, models and experience of the author.

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