Biogenic synthesis of selenium nanoparticles by Shewanella sp. HN-41 using modified bioelectrochemical system

Objective: The objective of this present study were to the biogenic synthesis of selenium nanoparticles using Aloe vera extract and check it’s antioxidant potential by ABTS, DPPH and FRAP assays.Methods: In this study we investigated the clove of Aloe vera, which is used for the synthesis of selenium nanoparticles were characterized by using UV-Visible (UV-VIS) spectrophotometer, Transmission electron microscopy (TEM), Fourier transform spectroscopy (FTIR) and Energy dispersive X-Ray spectroscopy (EDAX) and ABTS, DPPH and FRAP assays for checked it’s antioxidant potential.Results: The present study was carried out to synthesis of Selenium nanoparticles using extract of Aloe vera. UV-Vis Spectra at 350 nm with Aloe vera extract and observed as hollow and spherical particles in size ranging 7-48 nm which is found more stable more than two months. EDAX analysis was carried out to check the presoak of Selenium in nanoparticles. Results of EDAX, confirmed its present. TEM and SEAD represented addition evidence of formation of nanoparticles whereas SEAD indicates the particles were crystalline in nature. FT-IR analysis was carried out to identify the possible bio molecules and Aloe vera extract-metal ions interaction responsible for formation and stabilization of selenium nanoparticles. FRAP, ABTS and DPPH assay results sequester that Selenium nanoparticles prepared using Aloe vera extract possess more activity than extract alone.Conclusion: The bio molecules of Aloe veraextract acted as stabilizing as well as capping agent leading to the formation of Selenium nanoparticles. Selenite has been proven to have antioxidant activity and is being used as chemoprevention agent in cancer diagnosis but same time it is toxic also. Elemental Selenium i.e. Selenium nanoparticles are less toxic form of selenium.

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Biogenic Synthesis of Novel Functionalized Selenium Nanoparticles by Lactobacillus casei ATCC 393 and Its Protective Effects on Intestinal Barrier Dysfunction Caused by Enterotoxigenic Escherichia coli K88

Frontiers in Microbiology ◽

10.3389/fmicb.2018.01129 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 13

Author(s):

Chunlan Xu ◽

Yu Guo ◽

Lei Qiao ◽

Li Ma ◽

Yiyi Cheng ◽

...

Keyword(s):

Escherichia Coli ◽

Lactobacillus Casei ◽

Intestinal Barrier ◽

Selenium Nanoparticles ◽

Enterotoxigenic Escherichia Coli ◽

Protective Effects ◽

Barrier Dysfunction ◽

Biogenic Synthesis ◽

Intestinal Barrier Dysfunction

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Biogenic Synthesis of Selenium Nanoparticles and Their Effect on As(III)-Induced Toxicity on Human Lymphocytes

Biological Trace Element Research ◽

10.1007/s12011-014-9891-0 ◽

2014 ◽

Vol 157 (3) ◽

pp. 275-283 ◽

Cited By ~ 64

Author(s):

Kumar Suranjit Prasad ◽

Kaliaperumal Selvaraj

Keyword(s):

Human Lymphocytes ◽

Selenium Nanoparticles ◽

Biogenic Synthesis

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Biogenic Synthesis of Selenium Nanoparticles with Edible Mushroom Extract: Evaluation of Cytotoxicity on Prostate Cancer Cell Lines and Their Antioxidant, and Antibacterial Activity

Biointerface Research in Applied Chemistry ◽

10.33263/briac106.66296639 ◽

2020 ◽

Vol 10 (6) ◽

pp. 6629-6639 ◽

Cited By ~ 1

Keyword(s):

Prostate Cancer ◽

Cell Lines ◽

Spherical Shape ◽

Edible Mushroom ◽

Selenium Nanoparticles ◽

High Concentration ◽

Biogenic Synthesis ◽

Mushroom Extract ◽

Transmission Electron ◽

Uv Visible

We report edible mushroom extract (EME) as a robust and environmentally friendly precursor for the biogenic synthesis of selenium nanoparticles (SeNPs). The as-synthesized SeNPs were characterized by UV-visible spectrophotometer (UV-vis), Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscope (TEM), and Dynamic light scattering (DLS) techniques. The results obtained from TEM and DLS suggested that the SeNPs synthesized with EME are in the size of ~ 8 nm with a spherical shape and monodispersity. The occurrence of antioxidants in the EME has been confirmed with FTIR and thereby confirmed that flavonoids and phenolic compounds played a pivotal role in the biosynthesis of SeNPs from Na2SeO3. In addition, EME-SeNPs exhibited no cytotoxicity on the cell lines of prostate cancer (PC-3) at concentrations ranging from 0.5-1.5 μM. At a concentration of 0.25 mM against 1,1-diphenyl-2-picrylhydrazyl, the average scavenging level of EME-SeNPs was found to be > 80.20 %. Moreover, the percentage viability of gram-negative E.coli and gram-positive E.faecium was recorded at a high concentration of EME-SeNPs and found to be 82 % and 65 %, respectively.

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Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2019.12.5.4 ◽

2019 ◽

Vol 12 (5) ◽

pp. 4644-4647

Author(s):

K.K. Gupta ◽

Neha Kumari ◽

Neha Sinha ◽

Akruti Gupta

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Antimicrobial Agents ◽

Color Change ◽

Bacterial Species ◽

Antimicrobial Property ◽

Biogenic Synthesis ◽

E Coli ◽

Light Yellow ◽

Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

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Insight on Ameliorative Role of Selenium Nanoparticles and Niacin in Wound Healing on Adult Female Albino Mice

Current Chemical Biology ◽

10.2174/2212796814666200818111849 ◽

2020 ◽

Vol 14 (3) ◽

pp. 169-186

Author(s):

Marwa Emam ◽

Akaber T. Keshta ◽

Yasser M.A. Mohamed ◽

Yasser A. Attia

Keyword(s):

Wound Healing ◽

Adult Female ◽

Vascular Endothelial Cell ◽

Skin Layer ◽

Positive Control ◽

Healing Time ◽

Selenium Nanoparticles ◽

Tissue Samples ◽

Liver And Kidney ◽

Albino Mice

Background: Wound healing is a complex process necessary for repairing damaged tissues and preventing infection. Selenium nanoparticles (Se NPs) were known due to their antioxidant and antimicrobial effects, also niacin has angiogenesis and antioxidant effects that are important in wound healing. Objective: The present study was conducted to investigate the effect of Se NPs and niacin in reducing and accelerating the wound healing time in mice. Methods: A simple wet chemical method has been modified to synthesize Se NPs in order to investigate their effect and niacin on reducing the wound healing in 80 adult female albino mice (250 mm2 full thickness open excision wound) that were divided into eight groups (10 mice/each). After 30-days, the mice were sacrificed, blood and tissue samples were taken for analysis. Results: The results showed that the percentage of wound area had been significantly reduced in Se NPs and niacin treated groups compared to the positive control. The level of Vascular Endothelial cell Growth Factor and Collagenase I in Se NPs and niacin groups significantly exceed those of other groups while Nitric Oxide (NO) was significantly decreased in treated groups. Liver and kidney functions showed the lower toxicity effect of Se NPs and niacin. Skin tissue showed the wound healing effect of Se NPs and niacin by regenerating skin layer compared to the positive group. Conclusion: Se NPs and niacin play an important role in accelerating and reducing the time of wound healing while they were antagonistic to each other.

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