scholarly journals A new LC/MS method for specific determination of human systemic exposure to bisphenol A, F and S through their metabolites: Application to cord blood samples

2021 ◽  
Vol 151 ◽  
pp. 106429
Author(s):  
C.A. Gély ◽  
A. Huesca ◽  
N. Picard-Hagen ◽  
P.L. Toutain ◽  
A. Berrebi ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Cord Blood ◽  
Systemic Exposure ◽  
Blood Samples ◽  
Specific Determination

Determination of tissue plasminogen activator and its "fast" inhibitor in plasma.

1986 ◽  
Vol 32 (3) ◽  
pp. 482-485 ◽  
Author(s):  
J Chmielewska ◽  
B Wiman
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Plasma Samples ◽  
Inhibitor Concentration ◽  
Blood Samples ◽  
Analytical Recovery ◽  
Specific Determination ◽  
Tissue Plasminogen ◽  
Coupled Assay

Abstract We describe efficient, accurate methods for specific determination of tissue plasminogen activator (t-PA, EC 3.4.21.31) and its "fast" inhibitor in plasma. In this coupled assay, a sample containing t-PA is incubated with plasminogen, a plasmin (EC 3.4.21.7) substrate of low Km and high Kcat, and fibrin as a stimulator. The inhibitor of t-PA is determined by incubating the sample with a known amount of t-PA in excess, then determining the residual t-PA. Both t-PA and t-PA inhibitor can be determined in many samples simultaneously within a few hours. These assays are modifications of procedures described by us (Clin Chim Acta 1983;127:279-88 and Thromb Res 1983;31:427-36). Their accuracy as assessed by analytical recovery of pure t-PA added to blood samples (91 +/- 4%) or of partly purified inhibitor added to plasma samples (102 +/- 10%) is satisfactory, as is their precision. For the t-PA assay the CV was 1.6% (within run) or 4.1% (between run). The corresponding values for the inhibitor assay were 4.5% (within run) or 8.4% (between run) if the inhibitor concentration exceeded 3 arb. units/mL.

APPLICATION OF PIXE TO DETERMINATION OF ELEMENTS IN THE PLACENTA, MATERNAL BLOOD AND CORD BLOOD

1997 ◽  
Vol 07 (03n04) ◽  
pp. 211-217 ◽  
Author(s):  
Yoshito Watanabe ◽  
Yoshie Takubo ◽  
Masae Yukawa ◽  
Yoshikazu Nishimura ◽  
Hitoshi Imaseki ◽  
...  
Keyword(s):  
Cord Blood ◽  
Maternal Blood ◽  
Pixe Analysis ◽  
Blood Samples ◽  
Mean Values ◽  
The Mean ◽  
Standard Deviations ◽  
Homogenize Sample ◽  
Cu And Zn

PIXE technique was applied to the measurement of elements in the placenta, maternal blood and cord blood of human. The elements determined in these samples include Cl , K , Ca , Fe , Cu and Zn . The values obtained by PIXE were compared with those by ICP-AES to test the accuracy of the method. In placental samples, the mean values of K , Fe , Cu and Zn concentrations obtained by the two methods agree, while Ca concentration is lower in PIXE analysis. The values from PIXE, however, show larger variations resulting from inhomogeneity of the placental samples composed of various tissues different in histological functions. In the analysis of blood samples, the results of the two methods agree for Cl , K , Ca , Fe , Cu and Zn , although the standard deviations tend to be larger in PIXE. These results indicate that PIXE is a useful method for the determination of elements in placental and blood samples, although the preparation to homogenize sample is necessary to obtain accurate results.

scholarly journals Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan

2021 ◽  
Vol 18 (11) ◽  
pp. 6144
Author(s):  
Yu-Fang Huang ◽  
Chia-Huang Chang ◽  
Pei-Jung Chen ◽  
I-Hsuan Lin ◽  
Yen-An Tsai ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Birth Weight ◽  
Low Birth Weight ◽  
Bisphenol A ◽  
Cord Blood ◽  
Developmental Disorders ◽  
Metabolic Diseases ◽  
System Development ◽  
Maternal Blood ◽  
Blood Samples

Prenatal exposure to bisphenol A (BPA) may increase the risk of abnormal birth outcomes, and DNA methylation might mediate these adverse effects. This study aimed to investigate the effects of maternal BPA exposure on maternal and fetal DNA methylation levels and explore whether epigenetic changes are related to the associations between BPA and low birth weight. We collected urine and blood samples originating from 162 mother-infant pairs in a Taiwanese cohort study. We measured DNA methylation using the Illumina Infinium HumanMethylation 450 BeadChip in 34 maternal blood samples with high and low BPA levels based on the 75th percentile level (9.5 μg/g creatinine). Eighty-seven CpGs with the most differentially methylated probes possibly interacting with BPA exposure or birth weight were selected using two multiple regression models. Ingenuity pathway analysis (IPA) was utilized to narrow down 18 candidate CpGs related to disease categories, including developmental disorders, skeletal and muscular disorders, skeletal and muscular system development, metabolic diseases, and lipid metabolism. We then validated these genes by pyrosequencing, and 8 CpGs met the primer design score requirements in 82 cord blood samples. The associations among low birth weight, BPA exposure, and DNA methylation were analyzed. Exposure to BPA was associated with low birth weight. Analysis of the epigenome-wide findings did not show significant associations between BPA and DNA methylation in cord blood of the 8 CpGs. However, the adjusted odds ratio for the dehydrogenase/reductase member 9 (DHRS9) gene, at the 2nd CG site, in the hypermethylated group was significantly associated with low birth weight. These results support a role of BPA, and possibly DHRS9 methylation, in fetal growth. However, additional studies with larger sample sizes are warranted.

Placental Forms and Zygosity Determination of Twins in Ibadan, Western NigeriaA study of 1475 twin maternities

1970 ◽  
Vol 19 (1-2) ◽  
pp. 49-54 ◽  
Author(s):  
P. P. S. Nylander
Keyword(s):  
Cord Blood ◽  
Histological Examination ◽  
Twin Pair ◽  
Electrophoretic Pattern ◽  
Blood Groups ◽  
Gene Frequencies ◽  
Blood Samples ◽  
Registrar General ◽  
Very High

The incidence of twinning in Western Nigeria is very high, being 45‰ (Nylander, 1969a), as compared with 11-12‰ in Caucasian populations (Registrar General, 1967). A recent study of twinning in Ibadan, in which zygosity of individual twin pairs was determined, has shown that the pattern of placentation and zygosity in this population is also very different from that in Caucasian populations.As many as 1475 consecutive newborn twin pairs delivered in three hospitals in Ibadan between March 1967 and April 1969 were investigated.The placentae of the twins were examined macroscopically by the author personally to determine the form (whether single, fused, double or separate) and the membrane relationships (whether monochorionic or dichorionic) as shown in Figs. 1 and 2. The membrane relationship was confirmed by histological examination.Cord blood samples were collected from each twin pair and studied for (1) ABO, Rh (using antisera, C, c, D, E, e), MNSs and Gonzales blood groups and (2) G6PD electrophoretic pattern. Other blood groups like Fya, k and Jk, used initially, were discontinued later, because the gene frequencies in this population are such that they are uninformative for zygosity determination.Samples of the placenta of each twin were sent in refrigerated containers to the Galton Laboratory, London University, for placental enzyme studies — phosphoglucomutase and peptidase (Hopkinson and Harris, 1968; Lewis and Harris, 1967).

Synthesis and solvatochromic studies of 2- amino-3-phenolazo1-(4-sulfophenyl)-3-methyi-5-pyrozolone and use it for the determination of trace amount of Nickel (II) in blood samples

2016 ◽  
Vol 5 (10) ◽  
pp. 4920
Author(s):  
Amar M. Ali ◽  
Hussain. J. Mohammed*
Keyword(s):  
Stability Constant ◽  
Trace Amount ◽  
Benzenesulfonic Acid ◽  
Determination Method ◽  
Blood Samples ◽  
Determination Of Nickel ◽  
Normal Human ◽  
The Stability ◽  
Normal Human Blood

A new, simple, sensitive and rapid spectrophotometric method is proposed for the determination of trace amount of Nickel (II). The method is based on the formation of a 1:2 complex with 4-(4-((2-hydroxy-6-nitrophenyl) diazenyl) -3-methyl-5-oxo-2, 5-dihydro-1H-pyrazol-1-yl) benzenesulfonic acid (2-ANASP) as a new reagent is developed. The complex has a maximum absorption at 516 nm and εmax of 1. 84 X 105 L. mol-1. cm-1. A linear correlation (0. 25 – 4. 0μg. ml-1) was found between absorbance at λmax and concentration. The accuracy and reproducibility of the determination method for various known amounts of Nickel (II) were tested. The results obtained are both precise (RSD was 1. 2 %) and accurate (relative error was 0. 787 %). The effect of diverse ions on the determination of Nickel (II) to investigate the selectivity of the method were also studied. The stability constant of the product was 0. 399 X 106 L. mol-1. The proposed method was successfully applied to the analysis of diabetes blood and normal human blood. 

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