Effects of diazinon on acetylcholinesterase activity and lipid peroxidation in the brain of Oreochromis niloticus

Background: Ascorbic acid (AA) is a water soluble vitamin which is concentrated in the brain in large quantities. While there have been reports that it is essential for proper brain functioning, there is a dearth of information on the possible effects dietary fortification with AA on the brain in health. Objective: This study examined the effects of dietary fortification of rodent chow with AA on neurobehaviour, antioxidant status, lipid peroxidation, and inflammatory/apoptotic markers in the brain of healthy mice. Method: Mice were randomly-assigned into four groups of ten animals each. Groups were normal control [fed rodent chow] and three groups fed AA-fortified chow at 100, 200 and 300 mg/kg of feed respectively, for eight weeks. Behavioural tests Open field, Y-maze, radial-arm maze, and elevated plus maze (EPM) were carried out on day 57. Twenty-four hours after the last behavioural test, animals were euthanised and the brains were excised and homogenised for assessment of brain acetylcholinesterase activity, lipid peroxidation, antioxidant status, inflammatory and apoptotic markers. Results: Ascorbic acid fortified diet was associated with concentration-dependent changes in body weight, open-field behaviours, working-memory, and anxiety indices. Also, brain levels of malondialdehyde, caspase-3, and TNF-α decreased; while superoxide dismutase activity, total antioxidant capacity and IL-10 level increased. Conclusion: Dietary AA fortification with concentrations up to 300 mg/kg of feed was associated with sustained improvement in neurobehavioural and biochemical parameters in the brain of healthy mice reiterating additional health benefits of AA fortification beyond the prevention of nutritional deficiencies.

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17-β estradiol in the acetylcholinesterase activity and lipid peroxidation in the brain and blood of ovariectomized adult and middle-aged rats

Life Sciences ◽

10.1016/j.lfs.2011.12.006 ◽

2012 ◽

Vol 90 (9-10) ◽

pp. 351-359 ◽

Cited By ~ 13

Author(s):

Danieli Brolo Martins ◽

Cinthia Melazzo Mazzanti ◽

Raqueli Teresinha França ◽

Marciélen Pagnoncelli ◽

Márcio Machado Costa ◽

...

Keyword(s):

Lipid Peroxidation ◽

Acetylcholinesterase Activity ◽

Aged Rats ◽

Middle Aged ◽

The Brain

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Dissecting the localization of Tilapia tilapinevirus in the brain of the experimentally infected Nile tilapia, Oreochromis niloticus (L.)

Journal of Fish Diseases ◽

10.1111/jfd.13367 ◽

2021 ◽

Author(s):

Nguyen Dinh‐Hung ◽

Pattiya Sangpo ◽

Thanapong Kruangkum ◽

Pattanapon Kayansamruaj ◽

Tilladit Rung‐ruangkijkrai ◽

...

Keyword(s):

Oreochromis Niloticus ◽

Nile Tilapia ◽

Nile Tilapia Oreochromis Niloticus ◽

The Brain

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Inhibition of Acetylcholinesterase Activity and Fe2+-Induced Lipid Peroxidation in Rat Brain In Vitro by Some Citrus Fruit Juices

Journal of Medicinal Food ◽

10.1089/jmf.2011.0226 ◽

2012 ◽

Vol 15 (5) ◽

pp. 428-434 ◽

Cited By ~ 16

Author(s):

Ayokunle O. Ademosun ◽

Ganiyu Oboh

Keyword(s):

Lipid Peroxidation ◽

Rat Brain ◽

Citrus Fruit ◽

Acetylcholinesterase Activity ◽

Fruit Juices

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Effects of Cypermethrin on Antioxidant Enzyme Activities and Lipid Peroxidation in Liver and Kidney of the Freshwater Fish, Oreochromis niloticus and Cyprinus carpio (L.)

Bulletin of Environmental Contamination and Toxicology ◽

10.1007/s001280174 ◽

2001 ◽

Vol 67 (5) ◽

pp. 657-664 ◽

Cited By ~ 1

Author(s):

N. Üner ◽

E. Özcan Oruç ◽

M. Canli ◽

Y. Sevgler

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Enzyme ◽

Freshwater Fish ◽

Cyprinus Carpio ◽

Oreochromis Niloticus ◽

Enzyme Activities ◽

Antioxidant Enzyme Activities ◽

Liver And Kidney

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New Galantamine Derivatives with Inhibitory Effect on Acetylcholinesterase Activity

Journal of Alzheimer s Disease ◽

10.3233/jad-210577 ◽

2021 ◽

Vol 83 (3) ◽

pp. 1211-1220 ◽

Cited By ~ 1

Author(s):

Maria I. Lazarova ◽

Daniela S. Tsekova ◽

Lyubka P. Tancheva ◽

Kiril T. Kirilov ◽

Diamara N. Uzunova ◽

...

Keyword(s):

Lipid Peroxidation ◽

Memory Performance ◽

Acetylcholinesterase Activity ◽

Inhibitory Effect ◽

Long Term Memory ◽

Molegro Virtual Docker ◽

Ache Inhibitors ◽

Hybrid Molecules ◽

Ache Inhibitory Activity ◽

Biological Assessments

Background: Inhibitors of acetylcholinesterase (AChE) are used to treat many disorders, among which are neurodegenerative upsets, like Alzheimer’s disease (AD). One of the limited licensed AChE inhibitors (AChEIs) used as drugs is the natural compound galantamine (Gal). Objective: As Gal is a toxic compound, here we expose data about its four derivatives in hybrid peptide-norgalantamine molecules, which have shown 100 times lower toxicity. Methods: Four newly synthesized galantamine derivatives have been involved in docking analysis made by Molegro Virtual Docker. Biological assessments were performed on ICR male mice. The change in short and long-term memory performance was evaluated by passive avoidance test. AChE activity and levels of main oxidative stress parameters: lipid peroxidation, total glutathione (GSH), enzyme activities of catalase (CAT), superoxide dismutase, and glutathione peroxidase were measured in brain homogenates. Results: Our experimental data revealed that the new hybrid molecules did not impair memory performance in healthy mice. Two of the compounds demonstrated better than Gal AChE inhibitory activity in the brain. None of them changed the level of lipid peroxidation products, one of the compounds increased GSH levels, and all of them increased CAT enzyme activity. Conclusion: The new galantamine-peptide hybrids demonstrated a potential for inhibition of AChE and antioxidant activity and deserve further attention.

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Clinical significance of acetylcholinesterase activity in brain tumors

Russian Clinical Laboratory Diagnostics ◽

10.51620/0869-2084-2021-66-12-718-721 ◽

2021 ◽

Vol 66 (12) ◽

pp. 718-721

Author(s):

Larisa Mikhailovna Obukhova ◽

I. A. Medyanik ◽

K. N. Kontorshchikova ◽

S. A. Simagina ◽

L. T. Musaelyan ◽

...

Keyword(s):

Brain Tumors ◽

Clinical Significance ◽

Whole Blood ◽

Tumor Tissue ◽

Acetylcholinesterase Activity ◽

Bioinformatic Analysis ◽

Acetyl Cholinesterase ◽

Primary Tumors ◽

Grade Ii ◽

The Brain

It has been established that the non-neuronal cholinergic system is related to the oncogenesis which increases the attractiveness of its components as the promising markers of oncologic diseases. The purpose of this work is to evaluate the clinical significance of the analysis of the activity of acetyl cholinesterase as a new marker of gliomas. The activity of acetyl cholinesterase was assessed by photo colorimetric analysis according to the Hestrin method recalculating the activity of the enzyme in the tumor tissue per 1 g of protein, and in the blood - by 0.1 g of hemoglobin. The data obtained in the primary tumors of the brain (28) in the tissue of the brain of persons who died as a result of injury (6) and in whole blood of patients with gliomas (28) and practically healthy people (10) were compared with the use of a number of statistical programs. A significant decrease in the activity of acetyl cholinesterase in tumor tissue and in whole blood is revealed as the degree of anaplasia of tumors increases, starting with Grade II. It is for the first time that a significant direct correlation was noted showing the consistency between the decrease in the activity of acetyl cholinesterase in the tumor tissue of the brain and blood. Bioinformatic analysis showed the connection of the enzyme of acetyl cholinesterase with proteins of the PI3K-AKT and Notch signaling pathways providing antiapoptotic and proliferative effects. The found dependences provide new insights into understanding of the mechanisms of gliomas genesis and can be used for selection of new diagnostic markers of brain tumors.

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C60 Fullerene Prevents Restraint Stress-Induced Oxidative Disorders in Rat Tissues: Possible Involvement of the Nrf2/ARE-Antioxidant Pathway

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/2518676 ◽

2018 ◽

Vol 2018 ◽

pp. 1-17 ◽

Cited By ~ 18

Author(s):

Olga O. Gonchar ◽

Andriy V. Maznychenko ◽

Nataliya V. Bulgakova ◽

Inna V. Vereshchaka ◽

Tomasz Tomiak ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Protein Expression ◽

Restraint Stress ◽

Stress Condition ◽

Rat Tissues ◽

Stress Exposure ◽

The Brain ◽

Nrf2 Protein

The effects of C60FAS (50 and 500 μg/kg) supplementation, in a normal physiological state and after restraint stress exposure, on prooxidant/antioxidant balance in rat tissues were explored and compared with the effects of the known exogenous antioxidant N-acetylcysteine. Oxidative stress biomarkers (ROS, O2⋅−, H2O2, and lipid peroxidation) and indices of antioxidant status (MnSOD, catalase, GPx, GST, γ-GCL, GR activities, and GSH level) were measured in the brain and the heart. In addition, protein expression of Nrf2 in the nuclear and cytosol fractions as well as the protein level of antiradical enzyme MnSOD and GSH-related enzymes γ-GCLC, GPx, and GSTP as downstream targets of Nrf2 was evaluated by western blot analysis. Under a stress condition, C60FAS attenuates ROS generation and O2⋅− and H2O2 releases and thus decreases lipid peroxidation as well as increases rat tissue antioxidant capacity. We have shown that C60FAS supplementation has dose-dependent and tissue-specific effects. C60FAS strengthened the antiradical defense through the upregulation of MnSOD in brain cells and maintained MnSOD protein content at the control level in the myocardium. Moreover, C60FAS enhanced the GSH level and the activity/protein expression of GSH-related enzymes. Correlation of these changes with Nrf2 protein content suggests that under stress exposure, along with other mechanisms, the Nrf2/ARE-antioxidant pathway may be involved in regulation of glutathione homeostasis. In our study, in an in vivo model, when C60FAS (50 and 500 μg/kg) was applied alone, no significant changes in Nrf2 protein expression as well as in activity/protein levels of MnSOD and GSH-related enzymes in both tissues types were observed. All these facts allow us to assume that in the in vivo model, C60FAS affects on the brain and heart endogenous antioxidative statuses only during the oxidative stress condition.

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Decreased glucorticoid sensitivity as a factor of stressogenic shifts in the activity of monoamine oxidase, lipid peroxidation, and behavior in rats

Problems of Endocrinology ◽

10.14341/probl11779 ◽

2003 ◽

Vol 49 (5) ◽

pp. 48-51 ◽

Cited By ~ 1

Author(s):

I. A. Volchegorsky ◽

V. E. Tseilikman ◽

D. S. Smirnov ◽

S. A. Ship ◽

A. V. Borisenkov

Keyword(s):

Lipid Peroxidation ◽

Monoamine Oxidase ◽

Brain Tissue ◽

Behavioral Disorders ◽

Immobilization Stress ◽

Glucocorticoid Hormones ◽

And Behavior ◽

The Brain

Four episodes of immobilization stress cause a decrease in the sensitivity to glucocorticoid hormones, followed by anxiogenic behavioral disorders, enhanced monoamine oxidase-В (МАО-В) activity and simultaneously increased lipid peroxidation (LPO) in the brain tissue of rats. Concurrently, there is an increase in renal МАО-В activity, as well as renal and hepatic accumulation of LPO products. Administration of kenalog (2 mg/kg), a pharmacological analogue of glucocorticoid hormones, prevents the poststress МАО-В activation and LPO and attenuates anxiogenic behavioral disorders in the rats.

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