Comparing the efficacy of nutmeg essential oil and a chemical pesticide against Musca domestica and Chrysomya albiceps for selecting a new insecticide agent against synantropic vectors

2021 ◽  
Vol 225 ◽  
pp. 108104
Author(s):  
Luciana F. Cossetin ◽  
Eduarda M.T. Santi ◽  
Quelen I. Garlet ◽  
Antônio F.I.M. Matos ◽  
Tiago P. De Souza ◽  
...  
2021 ◽  
Vol 135 ◽  
pp. 111229
Author(s):  
Rokas Mickus ◽  
Gintarė Jančiukė ◽  
Vytautas Raškevičius ◽  
Valeryia Mikalayeva ◽  
Inga Matulytė ◽  
...  

2019 ◽  
Vol 54 ◽  
pp. 101352 ◽  
Author(s):  
Seyyed Mojtaba Mousavi ◽  
Seyyed Alireza Hashemi ◽  
Seeram Ramakrishna ◽  
Hossein Esmaeili ◽  
Sonia Bahrani ◽  
...  

2019 ◽  
Vol 48 (1) ◽  
pp. 46-60
Author(s):  
Cíntia De Campos Chaves ◽  
Manuela Campos ,Ferreira ◽  
Júlio Mendes

Chysomya albiceps and Musca domestica are important for forensic entomology, and human and animal health. This study analyzed the effects of the coexistence of C. albiceps and M. domestica reared in four different assays in two pig tissues, brain and intestine: assay 1, interaction between the larvae of the same age; assay 2, interaction between larvae of C. albiceps 24 hours older than larvae of M. domestica; assay 3, interaction between larvae of M. domestica 24 hours older than the larvae of C. albiceps; assay 4, larvae of both species were reared together in flasks with a small supply of food. Weight of larvae, growth time and imago emergence frequency were studied. C. albiceps responded better than M. domestica under most conditionstested. Larvae of C. albiceps responded better in mixed cultures (together with M. domestica) than in pure cultures (larvae of the same species). In contrast, M. domestica responded better when reared in pure cultures. Both species presented shorter growth times when their larvae were reared in intestine tissue with larvae 24 hours younger than the larvae of the concurrent species, compared to their respective growth times in pure cultures. The results confirmed that trophic interactions are relevant to the successful colonization of carrion by C. albiceps. Coexistence of the two species may result in changes in values of their biological components. The results also help to shed light on the biology of the two species in carrion.


Pharmaceutics ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 628
Author(s):  
Inga Matulyte ◽  
Giedre Kasparaviciene ◽  
Jurga Bernatoniene

Essential oils are volatile liquids which evaporate and lose their pharmacological effect when exposed to the environment. The aim of this study is to protect nutmeg essential oil from environmental factors by encapsulation (shell material, sodium alginate) and determine the influence of crosslinker concentration (2%, 5% calcium chloride), different emulsifiers (polysorbate 80, sucrose esters), and magnesium aluminometasilicate on microcapsule physical parameters, encapsulation efficiency (EE), swelling index (SI), and other parameters. Nutmeg essential oil (NEO)-loaded calcium alginate microcapsules were prepared by extrusion. The swelling test was performed with and without enzymes in simulated gastric, intestinal, and gastrointestinal media. This study shows that the crosslinker concentration has a significant influence on EE, with 2% calcium chloride solution being more effective than 5%, and capsules being softer with 2% crosslinker solution. Using sucrose esters, EE is higher when polysorbate 80 is used. The swelling index is nearly three times higher in an intestinal medium without enzymes than in the medium with pancreatin. Microcapsule physical parameters depend on the excipients: the hardest capsules were obtained with the biggest amount of sodium alginate; the largest with magnesium aluminometasilicate. Sucrose esters and magnesium aluminometasilicate are new materials used in extrusion.


2001 ◽  
Vol 188 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Cinthia Bittencourt Spricigo ◽  
Ariovaldo Bolzan ◽  
Ricardo Antonio Francisco Machado ◽  
Luiz Henrique Castelan Carlson ◽  
José Carlos Cunha Petrus

2007 ◽  
Vol 16 (4) ◽  
pp. 235-237 ◽  
Author(s):  
Carlos H. Marchiori ◽  
Alexandre S Leles ◽  
Selma A. De Carvalho ◽  
Renata F. Rodrigues

Espécies de parasitóides associados com moscas sinantrópicas foram coletadas com auxílio de iscas de fígado bovino no matadouro Alvorada em Itumbiara, Estado de Goiás, de março a dezembro de 2005. As pupas foram removidas da areia, após usar iscas de fígado como substrato para atrair as moscas. Elas foram individualizadas em cápsulas de gelatina até a emergência dos adultos de moscas ou de seus parasitóides. A prevalência total de parasitismo foi 15,3%. A freqüência absoluta, porcentagem e espécies de parasitóides coletados foram: 205 exemplares (2,3%) de Aphaereta sp., 173 (80,0%) de Brachymeria podagrica, 578 (15,7%) Nasonia vitripennis, um (0,5%) de Pachycrepoideus vindemmiae, dois (0,9%) de Spalangia sp. e um (0,5%) de Trybliographa sp. A porcentagem de parasitismo apresentada pelos parasitóides de Aphaereta sp., B. podagrica, N. vitripennis, P. vindemmiae, Spalangia sp., e Trybliographa sp. foi de 0,4%, 12,3%, 2,4%, 0,1%, 0,1% e 0,1%, respectivamente. As espécies de dípteros parasitados foram: Chrysomya albiceps, C. megacephala, Musca domestica, Oxysarcodexia thornax, Peckia chrysostoma e Sarcodexia lambens.


Foods ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 37 ◽  
Author(s):  
Inga Matulyte ◽  
Aiste Jekabsone ◽  
Lina Jankauskaite ◽  
Paulina Zavistanaviciute ◽  
Vytaute Sakiene ◽  
...  

Nutmeg (Myristica fragrans) essential oil has antimicrobial, antiseptic, antiparasitic, anti-inflammatory, and antioxidant properties. We have recently demonstrated that hydrodistillation of nutmeg essential oil by applying magnesium aluminometasilicate as an excipient significantly increases both the content and amount of bioactive substances in the oil and hydrolats. In this study, we aimed to compare the antioxidant, antimicrobial, and anti-inflammatory activity of hydrolats and essential oil obtained by hydrodistillation in the presence and absence of magnesium aluminometasilicate as an excipient. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method revealed that magnesium aluminometasilicate did not significantly improved antioxidant activity of both essential oil and hydrolat. Antibacterial efficiency was evaluated by monitoring growth of 15 bacterial strains treated by a range of dilutions of the essential oil and the hydrolats. Essential oil with an excipient completely inhibited the growth of E. faecalis, S. mutans (referent), and P. multocida, whereas the pure oil was only efficient against the latter strain. Finally, the anti-inflammatory properties of the substances were assessed in a fibroblast cell culture treated with viral dsRNR mimetic Poly I:C. The essential oil with an excipient protected cells against Poly I:C-induced necrosis more efficiently compared to pure essential oil. Also, both the oil and the hydrolats with aluminometasilicate were more efficient in preventing IL-6 release in the presence of Poly I:C. Our results show that the use of magnesium aluminometasilicate as an excipient might change and in some cases improve the biological activities of nutmeg essential oil and hydrolats.


2014 ◽  
Vol 4 (1) ◽  
pp. 51 ◽  
Author(s):  
Vania Maria Moreira Valente ◽  
Gulab Newandram Jham ◽  
Carolina Marangon Jardim ◽  
Onkar Dev Dhingra ◽  
Ion Ghiviriga

<p>Aiming to substitute toxic synthetic fungicides, the activity of nutmeg (<em>Myristica fragrans</em>) essential oil (EO, obtained by hydrodistillation) was investigated against two important storage fungi-<em>Aspergillus flavus</em> <em>A. ochraceus</em>. The activity of crude nutmeg EO was investigated using poison food assay (PFA). At a concentration of 0.1%, the EO inhibited <em>A. flavus</em> and <em>A. ochraceus</em> growth by 43 and 65%, respectively. At a concentration of 0.3 %, <em>A. flavus</em> and <em>A. ochraceus</em> inhibitions were 84 and 79%, respectively. The crude nutmeg EO on fractionation by preparative TLC-bioautography presented one band from which two pure compounds were isolated by semi-preparative normal-phase high performance liquid chromatography. Myristicin and safrole were identified by nuclear magnetic resonance (<sup>1</sup>H and <sup>13</sup>C) and gas chromatography-mass spectrometry. The relative % of myristicin and safrol in the crude EO was 10.8 and 2.9, respectively, determined by gas chromatography with a flame ionization detector. The crude EO, the isolated active fraction, isolated myristicin and standard myristicin presented similar activities against the two fungi at concentrations of 0.1 and 0.3% by PFA. Based on these results it is concluded that myristicin is the major antifungal in nutmeg EO against <em>A. flavus</em> and <em>A. ochraceus</em>.</p>


2015 ◽  
Vol 114 (3) ◽  
pp. 1229-1232 ◽  
Author(s):  
Anabella Gallardo ◽  
María Inés Picollo ◽  
Gastón Mougabure-Cueto

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