Hydrogen peroxide-induced changes in activities of membrane lipids-degrading enzymes and contents of membrane lipids composition in relation to pulp breakdown of longan fruit during storage

2019 ◽  
Vol 297 ◽  
pp. 124955 ◽  
Author(s):  
Yixiong Lin ◽  
Hetong Lin ◽  
Yihui Chen ◽  
Hui Wang ◽  
Mark A. Ritenour ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Membrane Lipids ◽  
Degrading Enzymes ◽  
Induced Changes

Hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in HepG2 cells

2020 ◽  
Vol 01 ◽  
Author(s):  
Ayşe Mine Yılmaz ◽  
Gökhan Biçim ◽  
Kübra Toprak ◽  
Betül Karademir Yılmaz ◽  
Irina Milisav ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Cell Viability ◽  
Hepg2 Cells ◽  
Proteasome Activity ◽  
Cell Cycle Phases ◽  
Induced Changes ◽  
And Oxidative Stress ◽  
Quercetin Treatment

Background: Different cellular responses influence the progress of cancer. In this study, we have investigated the effect of hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in human hepatocellular carcinoma (HepG2) cells. Methods: The effects of hydrogen peroxide and quercetin on cell viability, cell cycle phases and oxidative stress related cellular changes were investigated. Cell viability was assessed by WST-1 assay. Apoptosis rate, cell cycle phase changes and oxidative stress were measured by flow cytometry. Protein expressions of p21, p27, p53, NF-Kβ-p50 and proteasome activity were determined by Western blot and fluorometry, respectively. Results: Hydrogen peroxide and quercetin treatment resulted in decreased cell viability and increased apoptosis in HepG2 cells. Proteasome activity was increased by hydrogen peroxide but decreased by quercetin treatment. Conclusion: Both agents resulted in decreased p53 protein expression and increased cell death by different mechanisms regarding proteostasis and cell cycle phases.

The Importance of Rhamnolipid-Biosurfactant-Induced Changes in Bacterial Membrane Lipids of Bacillus subtilis for the Antimicrobial Activity of Thiosulfonates

2012 ◽  
Vol 65 (5) ◽  
pp. 534-541 ◽  
Author(s):  
Anna Sotirova ◽  
Tatyana Avramova ◽  
Stoyanka Stoitsova ◽  
Irina Lazarkevich ◽  
Vera Lubenets ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Membrane Lipids ◽  
Bacterial Membrane ◽  
Rhamnolipid Biosurfactant ◽  
Induced Changes

Effects of UV-C irradiation on lipid peroxidation markers during ripening of tomato (Lycopersicon esculentum L.) fruits

2000 ◽  
Vol 27 (2) ◽  
pp. 147 ◽  
Author(s):  
Essaid Ait Barka ◽  
Siamak Kalantari ◽  
Joseph Makhlouf ◽  
Joseph Arul
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Lycopersicon Esculentum ◽  
Fruit Ripening ◽  
Membrane Lipids ◽  
Antioxidative Enzyme ◽  
Second Phase ◽  
Repair Mechanism ◽  
Primary Target ◽  
Uv C

The effects of a hormic dose (3.7 kJ m−2) of UV-C (254 nm) on changes in fruit membrane lipids perox-idation markers during storage were determined using tomato (Lycopersicon esculentum L. cv. Trust) fruit. There were two distinct response phases following the treatment. A significant induction of lipid peroxidation markers (lipofuscin-like compounds, malondialdehyde, aldehydes, pentane, ethane, hydrogen peroxide, and efflux of elec-trolytes including potassium and calcium) occurred within the first 5 days. This induction suggests that the cell mem-brane was the primary target of UV-C irradiation. After this period, the level of all of these peroxidation markers become lower in UV-C-treated fruit than in control fruit, suggesting the induction of a defense or repair mechanism, probably involving production of antioxidants and activation of antioxidative enzyme. Within the second phase, any changes in lipid peroxidation activity reflected the fruit ripening / senescence process rather than the UV-C effect.

scholarly journals Cadmium-Induced Changes in Antioxidative Systems, Hydrogen Peroxide Content, and Differentiation in Scots Pine Roots

2001 ◽  
Vol 127 (3) ◽  
pp. 887-898 ◽  
Author(s):  
Andres Schützendübel ◽  
Peter Schwanz ◽  
Thomas Teichmann ◽  
Kristina Gross ◽  
Rosemarie Langenfeld-Heyser ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Scots Pine ◽  
Peroxide Content ◽  
Antioxidative Systems ◽  
Induced Changes

The roles of metabolism of membrane lipids and phenolics in hydrogen peroxide-induced pericarp browning of harvested longan fruit

2016 ◽  
Vol 111 ◽  
pp. 53-61 ◽  
Author(s):  
Yifen Lin ◽  
Hetong Lin ◽  
Yixiong Lin ◽  
Shen Zhang ◽  
Yihui Chen ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Membrane Lipids ◽  
Pericarp Browning

Ascorbic acid and hydrogen peroxide (Fenton's reagent) induced changes in gelatin systems

2003 ◽  
Vol 17 (3) ◽  
pp. 321-326 ◽  
Author(s):  
Asgar Farahnaky ◽  
David A Gray ◽  
John R Mitchell ◽  
Sandra E Hill
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Fenton’S Reagent ◽  
Fenton's Reagent ◽  
Induced Changes

Ethylene-induced changes in lignification and cell wall-degrading enzymes in the roots of mungbean (Vigna radiata) sprouts

2013 ◽  
Vol 73 ◽  
pp. 412-419 ◽  
Author(s):  
Wei-Na Huang ◽  
Hong-Kai Liu ◽  
Hua-Hua Zhang ◽  
Zhen Chen ◽  
Yang-Dong Guo ◽  
...  
Keyword(s):  
Cell Wall ◽  
Vigna Radiata ◽  
Cell Wall Degrading Enzymes ◽  
Degrading Enzymes ◽  
Induced Changes

scholarly journals Modulation of sensory perception by hydrogen peroxide enables Caenorhabditis elegans to find a niche that provides both food and protection from hydrogen peroxide

2021 ◽  
Author(s):  
Jodie A. Schiffer ◽  
Stephanie V. Stumbur ◽  
Maedeh Seyedolmohadesin ◽  
Yuyan Xu ◽  
William T. Serkin ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Caenorhabditis Elegans ◽  
Bacterial Communities ◽  
Food Preference ◽  
Sensory Perception ◽  
General Mechanism ◽  
Safe Environment ◽  
Degrading Enzymes ◽  
Bacterial Food

SummaryHydrogen peroxide (H2O2) is the most common chemical threat that organisms face. Here, we show that H2O2 alters the bacterial food preference of Caenorhabditis elegans, enabling the nematodes to find a safe environment with food. H2O2 induces the nematodes to leave food patches of laboratory and microbiome bacteria when those bacterial communities have insufficient H2O2-degrading capacity. The nematode’s behavior is directed by H2O2-sensing neurons that promote escape from H2O2 and by bacteria-sensing neurons that promote attraction to bacteria. However, the input for H2O2-sensing neurons is removed by bacterial H2O2-degrading enzymes and the bacteria-sensing neurons’ perception of bacteria is prevented by H2O2. The resulting cross-attenuation provides a general mechanism that ensures the nematode’s behavior is faithful to the lethal threat of hydrogen peroxide, increasing the nematode’s chances of finding a niche that provides both food and protection from hydrogen peroxide.

scholarly journals Proteomics reveals synergy between biomass degrading enzymes and inorganic Fenton chemistry in leaf-cutting ant colonies

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Morten Schiøtt ◽  
Jacobus J Boomsma
Keyword(s):  
Hydrogen Peroxide ◽  
Plant Cell Wall ◽  
Plant Biomass ◽  
Biomass Degradation ◽  
Ant Colonies ◽  
Fenton Chemistry ◽  
Degrading Enzymes ◽  
Biochemical Assays ◽  
Oxidized Iron ◽  
Leaf Cutting

The symbiotic partnership between leaf-cutting ants and fungal cultivars processes plant biomass via ant fecal fluid mixed with chewed plant substrate before fungal degradation. Here we present a full proteome of the fecal fluid of Acromyrmex leaf-cutting ants, showing that most proteins function as biomass degrading enzymes and that ca. 85% are produced by the fungus and ingested, but not digested, by the ants. Hydrogen peroxide producing oxidoreductases were remarkably common in the proteome, inspiring us to test a scenario in which hydrogen peroxide reacts with iron to form reactive oxygen radicals after which oxidized iron is reduced by other fecal-fluid enzymes. Our biochemical assays confirmed that these so-called Fenton reactions do indeed take place in special substrate pellets, presumably to degrade plant cell wall polymers. This implies that the symbiotic partnership manages a combination of oxidative and enzymatic biomass degradation, an achievement that surpasses current human bioconversion technology.

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