Recent advances in optical biosensors for specific detection of E. coli bacteria in food and water

Optical biosensors for bacteria detection by a peptidomimetic antimicrobial compound

The Analyst ◽

10.1039/c5an01717c ◽

2015 ◽

Vol 140 (22) ◽

pp. 7726-7733 ◽

Cited By ~ 15

Author(s):

Elena Tenenbaum ◽

Ester Segal

Keyword(s):

Bacteria Detection ◽

Optical Biosensors ◽

Label Free ◽

Antimicrobial Compound ◽

Porous Si ◽

E Coli ◽

Optical Transducer

A sensitive and label-free biosensor for E. coli detection, based on a peptidomimetic antimicrobial compound, which is tethered to a nanostructured porous Si optical transducer is presented.

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Elisa preliminary studies of immobilization and specific detection of bacterial strains

Romanian Journal of Ecology & Environmental Chemistry ◽

10.21698/rjeec.2020.209 ◽

2020 ◽

Vol 2 (2) ◽

pp. 64-69

Author(s):

Madalina Mihalache ◽

◽

Alina Banciu ◽

Lucian Ionescu ◽

Mihai Nita-Lazar

Keyword(s):

Monoclonal Antibody ◽

Fluorescence Intensity ◽

Polyclonal Antibody ◽

Specific Binding ◽

Negative Control ◽

Enzyme Linked Immunosorbent Assay ◽

Bacterial Strains ◽

Specific Detection ◽

E Coli ◽

Alexa Fluor

The paper aims to emphasize the specific detection of bacterial strains using enzyme-linked immunosorbent assay. The assay is based on the specific binding of polyclonal antibody anti-E. coli tagged with FITC to E.coli and monoclonal antibody anti-Ps. aeruginosa tagged with Alexa Fluor 647 tagged to Ps. aeruginosa and on subsequent enzymatic immunological demonstration of the conjugated enzyme. In this experiment, the negative control was the Salmonella enterica strain. The two antibodies had no interaction with the negative control, instead, they were specific for E. coli and Ps. aeruginosa strains. When both strains were in the same well, the fluorescence intensity given by the presence of E. coli was 2.3 times higher than that given by Ps. aeruginosa, and the intensity of fluorescence decreased if there are both bacterial strains in the wells.

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Recent Advances in MXene Nanocomposite-Based Biosensors

Biosensors ◽

10.3390/bios10110185 ◽

2020 ◽

Vol 10 (11) ◽

pp. 185 ◽

Cited By ~ 1

Author(s):

Jinho Yoon ◽

Minkyu Shin ◽

Joungpyo Lim ◽

Ji-Young Lee ◽

Jeong-Woo Choi

Keyword(s):

Inorganic Compounds ◽

High Sensitivity ◽

High Conductivity ◽

Optical Biosensors ◽

Electrochemical Biosensors ◽

Synthesis Methods ◽

Two Dimensional ◽

Recent Advances ◽

Sensitivity And Selectivity

The development of advanced biosensors with high sensitivity and selectivity is one of the most demanded concerns in the field of biosensors. To meet this requirement, up until now, numerous nanomaterials have been introduced to develop biosensors for achieving high sensitivity and selectivity. Among the latest nanomaterials attracting attention, MXene is one of the best materials for the development of biosensors because of its various superior properties. MXenes are two-dimensional inorganic compounds with few atomic layers that possess excellent characteristics including high conductivity and superior fluorescent, optical, and plasmonic properties. In this review, advanced biosensors developed on the basis of the MXene nanocomposite are discussed with the selective overview of recently reported studies. For this, introduction of the MXene including the definition, synthesis methods, and its properties are discussed. Next, MXene-based electrochemical biosensors and MXene-based fluorescent/optical biosensors are provided, which are developed on the basis of the exceptional properties of the MXene nanocomposite. This review will suggest the direction for use of the Mxene nanocomposite to develop advanced biosensors with high sensitivity and selectivity.

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Indoxyl-β-D-glucuronide, a novel chromogenic reagent for the specific detection and enumeration of Escherichia coli in environmental samples

Canadian Journal of Microbiology ◽

10.1139/m88-115 ◽

1988 ◽

Vol 34 (5) ◽

pp. 690-693 ◽

Cited By ~ 11

Author(s):

Arthur Newton Ley ◽

Raymond John Bowers ◽

Saul Wolfe

Keyword(s):

Escherichia Coli ◽

Large Scale ◽

Membrane Filter ◽

Chloroacetic Acid ◽

Specific Detection ◽

E Coli ◽

Filter Test ◽

Almost All ◽

First Time ◽

Specific Reagent

About 97% of Escherichia coli strains produce β-glucuronidase, but almost all other Enterobacteriaceae lack this enzyme. A D-glucopyranosiduronic acid (glucuronide) possessing a readily detectable β-linked aglycone should, therefore, constitute a specific reagent for the detection of this organism. For this purpose, the title compound has been synthesized for the first time. The synthesis proceeds in eight steps from readily available D-glucuronolactone, anthranilic acid, and chloroacetic acid and can be carried out on a large scale. The compound has the predicted properties: when included in the standard membrane filter test for the analysis of water, indoxyl-β-D-glucuronide allows specific detection of E. coli through the formation of blue colonies that are the result of rapid conversion of the liberated aglycone to indigo. The recovery of E. coli is easily measured and almost quantitative.

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Intimin Gene (eae) Subtype-Based Real-Time PCR Strategy for Specific Detection of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 in Cattle Feces

Applied and Environmental Microbiology ◽

10.1128/aem.03161-13 ◽

2013 ◽

Vol 80 (3) ◽

pp. 1177-1184 ◽

Cited By ~ 16

Author(s):

Delphine Bibbal ◽

Estelle Loukiadis ◽

Monique Kérourédan ◽

Carine Peytavin de Garam ◽

Franck Ferré ◽

...

Keyword(s):

Escherichia Coli ◽

Real Time ◽

Shiga Toxin ◽

Real Time Pcr ◽

Specific Detection ◽

Content Type ◽

E Coli ◽

Cattle Feces ◽

Pcr Assays

ABSTRACTShiga toxin-producingEscherichia coli(STEC) strains belonging to serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 are known to be associated with particular subtypes of the intimin gene (eae), namely, γ1, β1, ε, θ, and γ1, respectively. This study aimed at evaluating the usefulness of their detection for the specific detection of these five main pathogenic STEC serotypes in cattle feces. Using real-time PCR assays, 58.7% of 150 fecal samples were found positive for at least one of the four targetedeaesubtypes. The simultaneous presence ofstx,eae, and one of the five O group markers was found in 58.0% of the samples, and the five targetedstxpluseaeplus O genetic combinations were detected 143 times. However, taking into consideration the association betweeneaesubtypes and O group markers, the resultingstxpluseaesubtype plus O combinations were detected only 46 times. The 46 isolation assays performed allowed recovery of 22E. colistrains belonging to one of the five targeted STEC serogroups. In contrast, only 2 of 39 isolation assays performed on samples that were positive forstx,eaeand an O group marker, but that were negative for the correspondingeaesubtype, were successful. Characterization of the 24E. coliisolates showed that 6 were STEC, including 1 O157:H7, 3 O26:H11, and 2 O145:H28. The remaining 18 strains corresponded to atypical enteropathogenicE. coli(aEPEC). Finally, the more discriminatingeaesubtype-based PCR strategy described here may be helpful for the specific screening of the five major STEC in cattle feces.

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Monoclonal Antibodies to Lipopolysaccharide O Antigens of Enterohemorrhagic Escherichia coli Strains in Serogroups O26, O45, O103, O111, O121, and O145

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-597 ◽

2015 ◽

Vol 78 (7) ◽

pp. 1252-1258 ◽

Cited By ~ 1

Author(s):

BRIAN W. BROOKS ◽

CHERYL L. LUTZE-WALLACE ◽

BURTON BLAIS ◽

MARTINE GAUTHIER ◽

MYLÈNE DESCHÊNES

Keyword(s):

Escherichia Coli ◽

Monoclonal Antibodies ◽

Enterohemorrhagic Escherichia Coli ◽

Human Pathogens ◽

Specific Detection ◽

Coli Isolate ◽

E Coli ◽

O Antigens

Non-O157 enterohemorrhagic Escherichia coli in priority serogroups O26, O45, O103, O111, O121, and O145 are increasingly recognized as important human pathogens. In the present study, a panel of monoclonal antibodies (MAbs) to the lipopolysaccharide O antigens of E. coli in serogroups O26, O45, O103, O111, O121, and O145 was produced. The specificity was evaluated by examining the reactivity of the MAbs with 50 E. coli strains and 42 non–E. coli bacteria, and several MAbs highly specific for E. coli strains in each of the six non-O157 priority serogroups were identified. The use of these highly specific MAbs may be of considerable value for determining whether an E. coli isolate belongs to one of the six priority non-O157 serogroups, for developing specific detection assays for these organisms, and for characterizing the lipopolysaccharide O antigens of isolates in these serogroups.

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Aptamer-Conjugated Quantum Dot Optical Biosensors: Strategies and Applications

Chemosensors ◽

10.3390/chemosensors9110318 ◽

2021 ◽

Vol 9 (11) ◽

pp. 318

Author(s):

Dongmin Kim ◽

Seungmin Yoo

Keyword(s):

Quantum Dots ◽

Size Composition ◽

Optical Signal ◽

Optical Biosensors ◽

Specific Detection ◽

Practical Applications ◽

Highly Sensitive ◽

Good Biocompatibility ◽

Photochemical Stability ◽

New Generation

Quantum dots (QDs) represent the promising new generation of luminophores owing to their size-, composition-, and surface-dependent tunable photoluminescence (PL) and photochemical stability. The development of various QD composites with high PL and good biocompatibility has facilitated the use of aptamer-functionalized QD biosensors for highly sensitive and specific detection of molecules in clinical and environmental settings. In addition to describing the recent advances in aptamer-based QD biosensor technology for the detection of diverse chemicals and biomolecules, this review provides recent examples of sensing strategies based on optical signal enhancement and quenching of QDs. It also discusses potential strategies for the development of biosensors to widen their practical applications across various scientific and technological fields.

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What is motion? Recent advances in the study of molecular movement patterns of the peptidoglycan synthesis machines

Journal of Bacteriology ◽

10.1128/jb.00598-21 ◽

2021 ◽

Author(s):

Melissa Mae Lamanna ◽

Anthony T. Maurelli

Keyword(s):

Current Knowledge ◽

Single Particle Tracking ◽

Directed Motion ◽

E Coli ◽

Recent Advances ◽

Molecular Movement ◽

Growing Cell ◽

Movement Dynamics ◽

Tracking Technology

How proteins move through space and time is a fundamental question in biology. While great strides have been made towards a mechanistic understanding of protein movement, many questions remain. We discuss the biological implications of motion in the context of the peptidoglycan (PG) synthesis machines. We review systems in several bacteria, including Escherichia coli , Bacillus subtilis , and Streptococcus pneumoniae , and present a comprehensive view of our current knowledge regarding movement dynamics. Discrepancies are also addressed since “one size does not fit all”. For bacteria to divide, new PG is synthesized and incorporated into the growing cell wall by complex multi-protein nanomachines consisting of PG synthases (transglycosylases [TG] and/or transpeptidases [TP]) as well as a variety of regulators and cytoskeletal factors. Advances in imaging capabilities and labeling methods have revealed that these machines are not static but rather circumferentially transit the cell via directed motion perpendicular to the long axis of model rod-shaped bacteria such as E. coli and B. subtilis . The enzymatic activity of the TG:TPs drives motion in some species, while motion is mediated by FtsZ treadmilling in others. In addition, both directed and diffusive motion of the PG synthases has been observed using single particle tracking technology. Here, we examine the biological role of diffusion regarding transit. Lastly, findings regarding the monofunctional transglycosylases (RodA and FtsW) as well as the Class A PG synthases are discussed. This minireview serves to showcase recent advances, broach mechanistic unknowns, and stimulate future areas of study.

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Recent advances in glycosidase probes used in Escherichia coli detection

Current Medicinal Chemistry ◽

10.2174/0929867328999201224111615 ◽

2020 ◽

Vol 28 ◽

Author(s):

Xianhu Wei ◽

Ying Feng ◽

Minling Chen ◽

Shuhong Zhang ◽

Moutong Chen ◽

...

Keyword(s):

Escherichia Coli ◽

Detection Methods ◽

Global Public Health ◽

E Coli ◽

Recent Advances ◽

Pathogenic Escherichia Coli ◽

Challenges And Opportunities ◽

Group A ◽

Reporter Group ◽

Analytical Tools

Pathogenic Escherichia coli poses a serious threat to global public health and are especially dangerous with the increase of antibiotic resistance. β-Glucuronidase (GUS) and some other glycosidases can serve as useful biomarkers or indicators for detection of E. coli. The probes made up of a glycosyl residue (recognition group), a label or reporter group, and a linkage that is generally a direct glycosidic bond, are powerful analytical tools. Upon hydrolysis of the glycosidic linkages by the corresponding glycosidases, these probes irreversibly release detectable labels or reporter molecules. A variety of such glycosidase probes have been developed and applied for detection of E. coli or for the development of various corresponding detection methods. This paper provides an overview of recent advances in this field, covering the development and applications of chromogenic, fluorogenic, luminogenic, and electrochemical glycosidase substrates. The challenges and opportunities in the probe development for detection of E. coli are also discussed.

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