Inactivation of Salmonella spp. in wheat flour by 395 nm pulsed light emitting diode (LED) treatment and the related functional and structural changes of gluten

2020 ◽  
Vol 127 ◽  
pp. 108716 ◽  
Author(s):  
Lihui Du ◽  
Amritha Jaya Prasad ◽  
Michael Gänzle ◽  
M.S. Roopesh
2020 ◽  
Vol 121 ◽  
pp. 166-177 ◽  
Author(s):  
Samir Subedi ◽  
Lihui Du ◽  
Amritha Prasad ◽  
Barun Yadav ◽  
M.S. Roopesh

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 737 ◽  
Author(s):  
Joshua Hadi ◽  
Magdalena Dunowska ◽  
Shuyan Wu ◽  
Gale Brightwell

SARS-CoV-2 is a single-stranded RNA virus classified in the family Coronaviridae. In this review, we summarize the literature on light-based (UV, blue, and red lights) sanitization methods for the inactivation of ssRNA viruses in different matrixes (air, liquid, and solid). The rate of inactivation of ssRNA viruses in liquid was higher than in air, whereas inactivation on solid surfaces varied with the type of surface. The efficacy of light-based inactivation was reduced by the presence of absorptive materials. Several technologies can be used to deliver light, including mercury lamp (conventional UV), excimer lamp (UV), pulsed-light, and light-emitting diode (LED). Pulsed-light technologies could inactivate viruses more quickly than conventional UV-C lamps. Large-scale use of germicidal LED is dependent on future improvements in their energy efficiency. Blue light possesses virucidal potential in the presence of exogenous photosensitizers, although femtosecond laser (ultrashort pulses) can be used to circumvent the need for photosensitizers. Red light can be combined with methylene blue for application in medical settings, especially for sanitization of blood products. Future modelling studies are required to establish clearer parameters for assessing susceptibility of viruses to light-based inactivation. There is considerable scope for improvement in the current germicidal light-based technologies and practices.


2018 ◽  
Vol 43 (1) ◽  
pp. E19-E31 ◽  
Author(s):  
M Par ◽  
I Repusic ◽  
H Skenderovic ◽  
E Klaric Sever ◽  
D Marovic ◽  
...  

SUMMARY Objectives: To monitor the real-time changes in light transmittance during composite curing and to use transmittance data to determine the curing times required for a complete polymerization. Methods: Three conventional and three bulk fill composites were cured with two light-emitting diode curing units at layer thicknesses of 2 mm and 4 mm. The real-time light transmittance data were collected by a UV-Vis spectrometer in the wavelength range of 350-550 nm, plotted against time (t) and fitted to an exponential function f(t), whose first derivative ΔT(t) = df(t)/dt represented the rate of transmittance change. As the changing transmittance reflects structural changes that occur during polymerization, ΔT(t) > 0 was considered to indicate an ongoing polymerization, whereas ΔT(t) values approaching zero suggested a complete polymerization. This principle was used to determine times required for a complete polymerization (tcomplete) for each material/thickness/curing unit combination. Results: Light transmittance was significantly influenced by the material type, sample thickness, and curing unit, amounting to 2.9%-27.0% for the bulk fill and 0.7%-16.7% for the conventional composites. The values of tcomplete amounted to 15.3-23.3 seconds for the bulk fill composites at 2 mm, 20.2-33.3 seconds for the conventional composites at 2 mm, 26.9-42.1 seconds for the bulk fill composites at 4 mm, and 40.1-59.8 seconds for the conventional composites at 4 mm. Additionally, an exponential relationship was discovered between the light transmittance and tcomplete. Conclusions: Some of the tcomplete values considerably exceeded the curing times recommended by the manufacturers.


2017 ◽  
Vol 196 ◽  
pp. 130-138 ◽  
Author(s):  
Vinayak Ghate ◽  
Amit Kumar ◽  
Min-Jeong Kim ◽  
Woo-Suk Bang ◽  
Weibiao Zhou ◽  
...  

1995 ◽  
Vol 44 (3) ◽  
pp. 261-269 ◽  
Author(s):  
Daniel J. Tennessen ◽  
Raymond J. Bula ◽  
Thomas D. Sharkey

2008 ◽  
Vol 1130 (1) ◽  
pp. 300-304 ◽  
Author(s):  
Alexander M. Macmillan ◽  
Colin D. McGuinness ◽  
Kulwinder Sagoo ◽  
David McLoskey ◽  
John C. Pickup ◽  
...  

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 679 ◽  
Author(s):  
Amritha Prasad ◽  
Michael Gänzle ◽  
M. S. Roopesh

High intensity pulsed light emitting diode (LED) treatment is a novel approach to inactivate foodborne pathogens. The objective of this study was to evaluate the antibacterial potential of high intensity 365 (UV-A) and 395 nm (NUV-Vis) LED treatments against Escherichia coli and Salmonella enterica at high and low water activity (aw) conditions, and to understand the influence of different process parameters on their antibacterial efficacy. Bacteria at high (in phosphate buffer saline, PBS) and low aw (aw = 0.75) conditions were treated with both the LEDs with specific doses at a fixed distance from the LEDs. The 365 nm LED showed more effectiveness in reducing the dried bacteria compared to 395 nm LED. The dry E. coli showed more resistance to LED treatments compared to Salmonella. The 365 and 395 nm LED treatments with ~658 J/cm2 dose resulted in reductions of 0.79 and 1.76 log CFU/g of Salmonella, respectively, on 0.75 aw pet foods. The LED treatments increased the surface temperature, resulting in water loss in the treated samples. This study showed that the dose, duration of light exposure, bacterial strain, and aw played a major role in the antibacterial efficacy of the 365 and 395 nm LEDs.


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