scholarly journals Extraction and characterization of phytochemical compounds from araçazeiro (Psidium cattleianum) leaf: Putative antioxidant and antimicrobial properties

2020 ◽  
Vol 137 ◽  
pp. 109573 ◽  
Author(s):  
Giovana Paula Zandoná ◽  
Lucíola Bagatini ◽  
Natália Woloszyn ◽  
Juliane de Souza Cardoso ◽  
Jessica Fernanda Hoffmann ◽  
...  
Keyword(s):  
Antimicrobial Properties ◽  
Psidium Cattleianum ◽  
Phytochemical Compounds

scholarly journals Conditions to Prolonged Release of Microencapsulated Carvacrol on Alginate Films as Affected by Emulsifier Type and PH

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Silvia Matiacevich ◽  
Natalia Riquelme ◽  
María Lidia Herrera
Keyword(s):  
Storage Time ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Edible Film ◽  
Initial Time ◽  
Tween 20 ◽  
Prolonged Release ◽  
E Coli ◽  
Fresh Foods

Alginate from algal biomass is used as edible film and the incorporation of antimicrobial agents improves its performance to increase the shelf-life of fresh foods. However, environmental conditions and intrinsic properties of films influence their release. The aim of this study was to investigate the effect of the concentration and type of encapsulating agent and pH of emulsions on the physical and antimicrobial properties of alginate-carvacrol films. Films containing alginate, carvacrol as antimicrobial agent, and Tween 20 or trehalose (0.25 and 0.75% w/w) as encapsulating agents were obtained from suspensions at pH 4 and pH 8. Physical characterization of emulsions and films and antimicrobial properties (E. coliandB. cinerea) was evaluated. Results showed that droplets size depended on trehalose concentration, but emulsion stability depended on pH and type of encapsulating agent, being more stable samples with trehalose at pH 4. Although films with Tween 20 presented the highest opacity, they showed the best antimicrobial properties at initial time; however, during storage time, they lost their activity before samples with trehalose and relative humidity (RH) was the principal factor to influence their release. Therefore, sample formulated with 0.25% trehalose at pH 4 and stored at 75% RH had the best potential as edible film for fresh fruits.

scholarly journals Production and Characterization of Keratin/Tragacanth Gum Nanohydrogels for Drug Delivery in Medical Textiles

2021 ◽  
Vol 8 ◽  
Author(s):  
Nazanin Mansouri Shirazi ◽  
Niloofar Eslahi ◽  
Adeleh Gholipour-Kanani
Keyword(s):  
Disulfide Bonds ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Herbal Extract ◽  
Minimum Size ◽  
Mechanical Resistance ◽  
Tragacanth Gum ◽  
Medical Textiles

Keratin protein has been applied for biomedical applications due to its biocompatibility, biodegradability, mechanical resistance, and bioavailability. Tragacanth gum (TG) as a polysaccharide-based biopolymer has wound healing and antimicrobial properties. In this study, keratin was extracted from protein-based chicken feather by using reduction hydrolysis (sodium sulfide), and nanogels of keratin and TG composites at different ratios were produced by using the chemical cross-linking method. Then, cinnamon (5 and 10%) as an antibacterial herbal extract was added to the nanogels and coated on cotton fabric. The morphology and size of the composite nanogels, chemical structure, biological, and antibacterial properties were evaluated. According to DLS results, TGK2:1 (ratio of TG to keratin = 2:1) had the minimum size (80 nm) and PDI (0.1), and therefore, this sample was chosen as the optimum one. FESEM and TEM images showed the semi-spherical shape of the produced nanogels. FTIR spectra revealed the possible hydrogen bonding between the components, and the formation of disulfide bonds after the addition of hydrogen peroxide was confirmed by XPS. After loading cinnamon into the nanogels, an increase in size was observed from 80 nm for free-nanogel to 85 and 105 nm for 5 and 10% extract-loaded nanogels, respectively. Besides, more cinnamon was released from the treated fabrics by increasing time and cinnamon concentration. The antibacterial test exhibited good antibacterial properties against both Gram-positive and Gram-negative bacteria. Finally, MTT assay approved the biocompatibility of the produced nanogels for potential use in medical textiles.

scholarly journals Blood Compatibility—An Important but Often Forgotten Aspect of the Characterization of Antimicrobial Peptides for Clinical Application

2019 ◽  
Vol 20 (21) ◽  
pp. 5426
Author(s):  
Stephan Harm ◽  
Karl Lohner ◽  
Ute Fichtinger ◽  
Claudia Schildböck ◽  
Jennifer Zottl ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Chain Length ◽  
Blood Compatibility ◽  
Acyl Chain ◽  
Antimicrobial Properties ◽  
Polymyxin B ◽  
Acyl Chain Length ◽  
Human Blood Cells ◽  
Plasmatic Coagulation

Acylation of antimicrobial peptides mimics the structure of the natural lipopeptide polymyxin B, and increases antimicrobial and endotoxin-neutralizing activities. In this study, the antimicrobial properties of lactoferrin-based LF11 peptides as well as blood compatibility as a function of acyl chain length were investigated. Beyond the classical hemolysis test, the biocompatibility was determined with human leukocytes and platelets, and the influence of antimicrobial peptides (AMPs) on the plasmatic coagulation and the complement system was investigated. The results of this study show that the acylation of cationic peptides significantly reduces blood tolerance. With increasing acyl chain length, the cytotoxicity of LF11 peptides to human blood cells also increased. This study also shows that acylated cationic antimicrobial peptides are inactivated by the presence of heparin. In addition, it could be shown that the immobilization of LF11 peptides leads to a loss of their antimicrobial properties.

Isolation and Characterization of Soil Myxobacteria from Nepal

2019 ◽  
Vol 24 (2) ◽  
pp. 7-16
Author(s):  
Nabin Rana ◽  
Saraswoti Khadka ◽  
Bishnu Prasad Marasini ◽  
Bishnu Joshi ◽  
Pramod Poudel ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Rrna Gene ◽  
Isolation And Characterization ◽  
The Family ◽  
Global Concern ◽  
Bacteria And Fungi ◽  
Antimicrobial Metabolites

 Realizing myxobacteria as a potential source of antimicrobial metabolites, we pursued research to isolate myxobacteria showing antimicrobial properties. We have successfully isolated three strains (NR-1, NR-2, NR-3) using the Escherichia coli baiting technique. These isolates showed typical myxobacterial growth characteristics. Phylogenetic analysis showed that all the strains (NR-1, NR-2, NR-3) belong to the family Archangiaceae, suborder Cystobacterineae, and order Myxococcales. Furthermore, 16S rRNA gene sequence similarity searched through BLAST revealed that strain NR-1 showed the closest similarity (91.8 %) to the type strain Vitiosangium cumulatum (NR-156939), NR-2 showed (98.8 %) to the type of Cystobacter badius (NR-043940), and NR-3 showed the closest similarity (83.5 %) to the type of strain Cystobacter fuscus (KP-306730). All isolates showed better growth in 0.5-1 % NaCl and pH around 7.0, whereas no growth was observed at pH 9.0 and below 5.0. All strains showed better growth at 32° C and hydrolyzed starch, whereas casein was efficiently hydrolyzed by NR-1 and NR-2. Besides, preliminary antimicrobial tests from crude extracts showed activities against Gram-positive, Gram-negative bacteria, and fungi. Our findings suggest that the arcane soil habitats of Nepal harbor myxobacteria with the capability to produce diverse antimicrobial activities that may be explored to overcome the rapidly rising global concern about antibiotic resistance.

Characterization of microemulsions formulated from naturally and chemically extracted oils from Cocos nucifera Linne

2020 ◽  
Vol 16 (1) ◽  
pp. 39-48
Author(s):  
O.A. Adetunji ◽  
B. Adesanwo ◽  
O.A. Odeniyi
Keyword(s):  
Castor Oil ◽  
Cocos Nucifera ◽  
Antimicrobial Properties ◽  
Coconut Oil ◽  
Tween 80 ◽  
Antimicrobial Activities ◽  
Oil Extraction ◽  
Hot Water ◽  
Copper Zinc

Background: Oil derived from Cocos nucifera Linne (CNL) has been used in formulating creams; however, its use in formulation of microemulsion is not well documented in literature.Objective: Oils obtained from CNL were characterized, used in the formulation of microemulsions and were evaluated for their antimicrobial properties in comparison with Castor oil BP (CO) and commercially available coconut oil (CACO).Methodology: Oil from CNL was extracted by maceration in hot – water and petroleum ether to yield naturally extracted oil (NEO) and chemically extracted oil (CEO) respectively. The NEO and CEO were used in formulating microemulsions containing pre-determined ratios (3:7, 1:1, 9:1) of oil to surfactant mix (Smix) [containing polyethylene glycol: Tween 80® at ratio 2:1] Characterization of NEO, CEO and microemulsions were carried out using elemental constituents, rheology, physico-and phyto-chemical analysis, thermal stability and antimicrobial profiles as parameters. Statistical analysis was done using ANOVA at p<0.05Results: The NEO and CEO contained antraquinones and terpenoids, but were devoid of lead, copper, zinc, alkaloids, saponins, cardiac glycosides and flavonoids. Viscosity profiles were in the order CO>CEO>CACO>NEO. The microemulsions were in the size range 54.24±0.26-89.08±0.07 μm. Microemulsions of oil:Smix (3:7) were the most stable. Inhibition ranking was Candida sp.D25 (CEO:Smix>CACO: Smix>NEO: Smix>NEO>CO>CEO), Candida sp.D33 (CEO = CO>CACO: Smix=NEO>CEO:Smix=NEO:Smix), Staphylococcus sp.DS2 (NEO>CO>CACO:Smix>NEO: Smix>CEO: Smix=CEO) and Pseudomonas sp.DP8 (NEO:Smix>CO>CACO:Smix>CEO:Smix=NEO > CEO).Conclusion: Oil derived from CNL has good potentials as an excipient in the formulation of microemulsions and the method of oil extraction had a significant effect on the antimicrobial activities and on the microemulsions formulated using the oils. Keywords: Coconut oil, extraction method, castor oil, microemulsions, antimicrobial properties.

Preparation and characterization of bacterial cellulose-poly(vinyl alcohol) films with antimicrobial properties

2012 ◽  
Vol 66 (1) ◽  
pp. 125-127 ◽  
Author(s):  
Iuliana Mihaela Jipa ◽  
Loredana Dobre ◽  
Marta Stroescu ◽  
Anicuta Stoica-Guzun ◽  
Sorin Jinga ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Vinyl Alcohol ◽  
Antimicrobial Properties ◽  
Poly Vinyl Alcohol
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