Widely targeted metabolomics analysis reveals new biomarkers and mechanistic insights on chestnut (Castanea mollissima Bl.) calcification process

2021 ◽  
Vol 141 ◽  
pp. 110128
Author(s):  
Jiaqi Xiao ◽  
Caiqin Gu ◽  
Shan He ◽  
Dongxue Zhu ◽  
Yukai Huang ◽  
...  
Keyword(s):  
Targeted Metabolomics ◽  
Castanea Mollissima ◽  
Widely Targeted Metabolomics ◽  
Calcification Process ◽  
New Biomarkers ◽  
Metabolomics Analysis

scholarly journals Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

2021 ◽  
Vol 8 ◽  
Author(s):  
Ziping Ai ◽  
Yue Zhang ◽  
Xingyi Li ◽  
Wenling Sun ◽  
Yanhong Liu
Keyword(s):  
Pathway Analysis ◽  
Thermal Processing ◽  
Fatty Acid Biosynthesis ◽  
Targeted Metabolomics ◽  
Transformation Mechanism ◽  
Metabolic Pathway Analysis ◽  
Active Compounds ◽  
Cistanche Deserticola ◽  
Widely Targeted Metabolomics ◽  
Metabolomics Analysis

Cistanche deserticola is one of the most precious plants, traditionally as Chinese medicine, and has recently been used in pharmaceutical and healthy food industries. Steaming and drying are two important steps in the processing of Cistanche deserticola. Unfortunately, a comprehensive understanding of the chemical composition changes of Cistanche deserticola during thermal processing is limited. In this study, ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based widely targeted metabolomics analysis was used to investigate the transformation mechanism of Cistanche deserticola active compounds during steaming and drying processes. A total of 776 metabolites were identified in Cistanche deserticola during thermal processing, among which, 77 metabolites were differentially regulated (p < 0.05) wherein 39 were upregulated (UR) and 38 were downregulated (DR). Forty-seven (17 UR, 30 DR) and 30 (22 UR, 8 DR) differential metabolites were identified during steaming and drying, respectively. The most variation of the chemicals was observed during the process of steaming. Metabolic pathway analysis indicated that phenylpropanoid, flavonoid biosynthesis, and alanine metabolism were observed during steaming, while glycine, serine, and threonine metabolism, thiamine metabolism, and unsaturated fatty acid biosynthesis were observed during drying. The possible mechanisms of the chemical alterations during thermal processing were also provided by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Furthermore, the blackening of the appearance of Cistanche deserticola mainly occurred in the steaming stage rather than the drying stage, which is associated with the metabolism of the amino acids. All results indicated that the formation of active compounds during the processing of Cistanche deserticola mainly occurred in the steaming stage.

scholarly journals Widely Targeted Metabolomics Analysis Reveals Key Quality-Related Metabolites in Kernels of Sweet Corn

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Ruichun Yang ◽  
Yunfeng Li ◽  
Yuanyuan Zhang ◽  
Jun Huang ◽  
Junjie Liu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Sweet Corn ◽  
Quinic Acid ◽  
Essential Amino Acids ◽  
Free Form ◽  
Targeted Metabolomics ◽  
Widely Targeted Metabolomics ◽  
Corn Kernels ◽  
Metabolomics Analysis

Sweet corn (Zea mays convar. saccharata var. rugosa) is a major economic vegetable crop. Different sweet corn cultivars vary largely in flavor, texture, and nutrition. The present study performed widely targeted metabolomics analysis based on the HPLC-MS/MS technology to analyze the metabolic profiles in three sweet corn cultivars widely grown in China. A total of 568 metabolites in the three sweet corn cultivars were detected, of which 262 differential metabolites significantly changed among cultivars. Carbohydrates, organic acids, and amino acids were the majority detected primary metabolites. Organic acids were mainly concentrated on shikimate, benzoic acids, and quinic acid with aromatic groups. And the essential amino acids for the human body, methionine and threonine, were highly accumulated in the high-quality cultivar. In addition, phenylpropanoids and alkaloids were the most enriched secondary metabolites while terpenes were low-detected in sweet corn kernels. We found that the flavonoids exist in both free form and glycosylated form in sweet corn kernels. PCA and HCA revealed clear separations among the three sweet corn cultivars, suggesting distinctive metabolome profiles among three cultivars. The differential metabolites were mapped into flavonoid biosynthesis, phenylpropanoid biosynthesis, biosynthesis of amino acids, and other pathways according to the KEGG classification. Furthermore, we identified skimmin, N ′ ,N ″ -diferuloylspermidine, and 3-hydroxyanthranilic acid as the key quality-related metabolites related to grain quality traits in sweet corn. The results suggested variations of metabolic composition among the three cultivars, providing the reference quality-related metabolites for sweet corn breeding.

scholarly journals Exercise-Induced Alterations in Skeletal Muscle, Heart, Liver, and Serum Metabolome Identified by Non-Targeted Metabolomics Analysis

Metabolites ◽  
2017 ◽  
Vol 7 (3) ◽  
pp. 40 ◽  
Author(s):  
Joseph Starnes ◽  
Traci Parry ◽  
Sara O’Neal ◽  
James Bain ◽  
Michael Muehlbauer ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Targeted Metabolomics ◽  
Exercise Induced ◽  
Metabolomics Analysis

scholarly journals Extensive Metabolite Profiling in the Unexploited Organs of Black Tiger for Their Potential Valorization in the Pharmaceutical Industry

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 544
Author(s):  
Jianfei Gao ◽  
Kangning Xiong ◽  
Wei Zhou ◽  
Weijie Li
Keyword(s):  
Acid Metabolism ◽  
Metabolite Profile ◽  
Industrial Applications ◽  
Targeted Metabolomics ◽  
Arginine Biosynthesis ◽  
Nutritional Values ◽  
Phenylpropanoid Biosynthesis ◽  
Tyrosine Metabolism ◽  
Organ Specific ◽  
Widely Targeted Metabolomics

Black tiger (Kadsura coccinea (Lem.)) has been reported to hold enormous pharmaceutical potential. The fruit and rhizome of black tiger are highly exploited in the pharmaceutical and other industries. However, the most important organs from the plant such as the leaf and stem are considered biowastes mainly because a comprehensive metabolite profile has not been reported in these organs. Knowledge of the metabolic landscape of the unexploited black tiger organs could help identify and isolate important compounds with pharmaceutical and nutritional values for a better valorization of the species. In this study, we used a widely targeted metabolomics approach to profile the metabolomes of the K. coccinea leaf (KL) and stem (KS) and compared them with the root (KR). We identified 642, 650 and 619 diverse metabolites in KL, KS and KR, respectively. A total of 555 metabolites were mutually detected among the three organs, indicating that the leaf and stem organs may also hold potential for medicinal, nutritional and industrial applications. Most of the differentially accumulated metabolites between organs were enriched in flavone and flavonol biosynthesis, phenylpropanoid biosynthesis, arginine and proline metabolism, arginine biosynthesis, tyrosine metabolism and 2-oxocarboxylic acid metabolism pathways. In addition, several important organ-specific metabolites were detected in K. coccinea. In conclusion, we provide extensive metabolic information to stimulate black tiger leaf and stem valorization in human healthcare and food.

scholarly journals Changes in Metabolome and Nutritional Quality of Lycium barbarum Fruits from Three Typical Growing Areas of China as Revealed by Widely Targeted Metabolomics

Metabolites ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 46 ◽  
Author(s):  
Yajun Wang ◽  
Xiaojie Liang ◽  
Yuekun Li ◽  
Yunfang Fan ◽  
Yanlong Li ◽  
...  
Keyword(s):  
Soil Moisture ◽  
Light Intensity ◽  
Nutritional Quality ◽  
Climatic Factors ◽  
Northwest China ◽  
High Light Intensity ◽  
Climatic Conditions ◽  
Targeted Metabolomics ◽  
Lycium Barbarum ◽  
Widely Targeted Metabolomics

This study aimed at assessing the climatic factors influencing the wolfberry fruit morphology, and the composition of its nutritious metabolites. The cultivar Ningqi1, widely grown in Northwest China was collected from three typical ecological growing counties with contrasting climatic conditions: Ningxia Zhongning (NF), Xinjiang Jinghe (XF) and Qinghai Nomuhong (QF). During the ripening period, 45 fruits from different plantations at each location were sampled. A total of 393 metabolites were detected in all samples through the widely targeted metabolomics approach and grouped into 19 known classes. Fruits from QF were the biggest followed by those from XF and NF. The altitude, relative humidity and light intensity had negative and strong correlations with most of the metabolites, suggesting that growing wolfberry in very high altitudes and under high light intensity is detrimental for the fruit nutritional quality. Soil moisture content is highly and negatively correlated with vitamins, organic acids and carbohydrates while moderately and positively correlated with other classes of metabolites. In contrast, air and soil temperatures exhibited positive correlation with majority of the metabolites. Overall, our results suggest high soil and air temperatures, low altitude and light intensity and moderate soil moisture, as the suitable conditions to produce Lycium fruits with high content of nutritious metabolites.

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