Fish cell cultures as in vitro models of pro-inflammatory responses elicited by immunostimulants

Two hepatoma cell cultures were examined as in vitro models to be used in genotoxicity and cytotoxicity tests without the addition of bioactivating enzymes. The MH1C1, and HTC hepatoma lines were used in this study to establish their sensitivity to a number of xenobiotics, namely, cyclophosphamide (CP), the classical positive control in bioactivation tests; benzaldehyde (BA), a short-chain aldehyde; and 4-hydroxynonenal (HNE), a major toxic end-product of the peroxidative degradation of cell membrane lipids. As a first approach, we compared the following cytotoxicity tests: release of lactate dehydrogenase (LDH), and colony formation efficiency (CF). Colony-forming cells were exposed to the drugs according to different procedures, before or after the anchorage phase. The leakage of LDH into the medium following exposure of both cell lines to HNE, CP and BA for up to 24 hours was found not to be a good index of cytotoxicity. A better indicator of cytotoxicity was CF, as evaluated by exposure of the cells 24 hours after seeding. The effects were detectable at very low concentrations, corresponding to 10, 90 and 100μM for HNE, CP and BA, respectively. The impairment of CF efficiency was dose-dependent and time-dependent, and several differences between the two cell lines were observed.

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The curious case of how mimicking physiological complexity in in vitro models of the human respiratory system influences the inflammatory responses. A preliminary study focused on gold nanoparticles

Journal of Interdisciplinary Nanomedicine ◽

10.1002/jin2.25 ◽

2017 ◽

Vol 2 (2) ◽

pp. 110-130 ◽

Cited By ~ 4

Author(s):

Dania Movia ◽

Luisana Di Cristo ◽

Roaa Alnemari ◽

Joseph E. McCarthy ◽

Hanane Moustaoui ◽

...

Keyword(s):

Gold Nanoparticles ◽

Respiratory System ◽

Inflammatory Responses ◽

In Vitro Models ◽

Preliminary Study ◽

Physiological Complexity ◽

Human Respiratory System

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Chitosan biopolymer: Alternative adhesion factor and scaffold matrix for 2D and 3D neuronal cultures

Biomedical Science and Engineering ◽

10.4081/bse.2019.107 ◽

2020 ◽

Author(s):

Donatella Di Lisa ◽

Mariateresa Tedesco ◽

Elena Dellacasa ◽

Mattia Pesce ◽

Tiziano Catelani ◽

...

Keyword(s):

Cell Cultures ◽

Culture Media ◽

In Vitro Models ◽

Neuronal Cultures ◽

Culture Techniques ◽

Chitosan Biopolymer ◽

2D And 3D ◽

Adhesion Factor

The increase of different types of cell cultures, which can be used for the in vitro studies of physiological and/or pathological processes, has introduced the need to improve culture techniques through the use of materials and culture media that promote growth, recreating a cellular micro-environment that can be asserted in in vivo condition. The standard methods for the functionalization of supports used for cell cultures are based on the use of synthetic or natural biopolymers, which generally have high costs, such as poly-lysine and polyornithine. The aim of this work is to demonstrate the alternative use of the polysaccharide chitosan as adhesion factor and structural component for 2D/3D neuronal cultures. Thanks to its versatility, it could be easily functionalized for the fabrication of personalized of in vitro models

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Lipid Annotation by Combination of UHPLC-HRMS (MS), Molecular Networking, and Retention Time Prediction: Application to a Lipidomic Study of In Vitro Models of Dry Eye Disease

Metabolites ◽

10.3390/metabo10060225 ◽

2020 ◽

Vol 10 (6) ◽

pp. 225 ◽

Cited By ~ 1

Author(s):

Romain Magny ◽

Anne Regazzetti ◽

Karima Kessal ◽

Gregory Genta-Jouve ◽

Christophe Baudouin ◽

...

Keyword(s):

Dry Eye ◽

Retention Time ◽

Inflammatory Responses ◽

In Vitro Models ◽

Dry Eye Disease ◽

Molecular Networks ◽

Chromatographic Behavior ◽

Eye Disease ◽

Molecular Networking

Annotation of lipids in untargeted lipidomic analysis remains challenging and a systematic approach needs to be developed to organize important datasets with the help of bioinformatic tools. For this purpose, we combined tandem mass spectrometry-based molecular networking with retention time (tR) prediction to annotate phospholipid and sphingolipid species. Sixty-five standard compounds were used to establish the fragmentation rules of each lipid class studied and to define the parameters governing their chromatographic behavior. Molecular networks (MNs) were generated through the GNPS platform using a lipid standards mixture and applied to lipidomic study of an in vitro model of dry eye disease, i.e., human corneal epithelial (HCE) cells exposed to hyperosmolarity (HO). These MNs led to the annotation of more than 150 unique phospholipid and sphingolipid species in the HCE cells. This annotation was reinforced by comparing theoretical to experimental tR values. This lipidomic study highlighted changes in 54 lipids following HO exposure of corneal cells, some of them being involved in inflammatory responses. The MN approach coupled to tR prediction thus appears as a suitable and robust tool for the discovery of lipids involved in relevant biological processes.

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Use of cell cultures to differentiate among effects of various ischemia factors on astrocytic cell volume

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y92-281 ◽

1992 ◽

Vol 70 (S1) ◽

pp. S344-S349 ◽

Cited By ~ 7

Author(s):

Bernhard H. J. Juurlink ◽

Ye Chen ◽

Leif Hertz

Keyword(s):

Cell Volume ◽

Cell Cultures ◽

Culture Medium ◽

In Vitro Models ◽

Similar Amount ◽

Sodium Potassium ◽

Astrocytic Swelling ◽

Per Se ◽

Substrate Deprivation

Mouse astrocytes were subjected to in vitro models of ischemia (hypoxia with or without substrate deprivation, excess potassium, or elevated glutamate). Three hours of hypoxia alone or with substrate deprivation had little effect upon the morphology of astrocytes but did cause disaggregation of polyribosomes. Excess (12–50 mM) potassium added (as KCl) to a normal isotonic medium also caused no swelling; it did, however, cause a shrinkage of cell volume. When 50 mM potassium was substituted for a similar amount of sodium, marked swelling occurred. Swelling of astrocytes was also seen after addition of glutamate (50 μM to 1 mM) to the culture medium. These results show that ischemia per se does not result in astrocytic swelling; rather, microenvironmental alterations such as rising glutamate levels and changes in the sodium/potassium ratios result in astrocytic swelling. We conclude that one can use astrocytes in culture to dissect out the mechanisms that cause postischemic alterations in astrocytes in vivo.Key words: astrocytes, glutamate, ischemia, potassium, swelling.

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In vitro models of periodontal cells: a comparative study of long-term gingival, periodontal ligament and alveolar bone cell cultures in the presence of β-glycerophosphate and dexamethasone

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-007-0134-1 ◽

2007 ◽

Vol 18 (6) ◽

pp. 1079-1088 ◽

Cited By ~ 11

Author(s):

Maria Cristina Trigo Cabral ◽

Maria Adelina Costa ◽

Maria Helena Fernandes

Keyword(s):

Comparative Study ◽

Cell Cultures ◽

Periodontal Ligament ◽

Alveolar Bone ◽

Bone Cell ◽

In Vitro Models ◽

Bone Cell Cultures

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The Effective Combination between 3D Cancer Models and Stimuli-Responsive Nanoscale Drug Delivery Systems

Cells ◽

10.3390/cells10123295 ◽

2021 ◽

Vol 10 (12) ◽

pp. 3295

Author(s):

Federica Foglietta ◽

Loredana Serpe ◽

Roberto Canaparo

Keyword(s):

Drug Delivery ◽

Cancer Cell ◽

Cell Cultures ◽

Drug Delivery Systems ◽

In Vitro Models ◽

Delivery Systems ◽

Stimuli Responsive ◽

Cancer Models

Stimuli-responsive drug-delivery systems (DDSs) have emerged as a potential tool for applications in healthcare, mainly in the treatment of cancer where versatile nanocarriers are co-triggered by endogenous and exogenous stimuli. Two-dimensional (2D) cell cultures are the most important in vitro model used to evaluate the anticancer activity of these stimuli-responsive DDSs due to their easy manipulation and versatility. However, some limitations suggest that these in vitro models poorly predict the outcome of in vivo studies. One of the main drawbacks of 2D cell cultures is their inadequate representation of the 3D environment’s physiological complexity, which sees cells interact with each other and the extracellular matrix (ECM) according to their specific cellular organization. In this regard, 3D cancer models are a promising approach that can overcome the main shortcomings of 2D cancer cell cultures, as these in vitro models possess many peculiarities by which they mimic in vivo tumors, including physiologically relevant cell–cell and cell–ECM interactions. This is, in our opinion, even more relevant when a stimuli-responsive DDS is being investigated. In this review, we therefore report and discuss endogenous and exogenous stimuli-responsive DDSs whose effectiveness has been tested using 3D cancer cell cultures.

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