Lethal effects of high-intensity violet 405-nm light on Saccharomyces cerevisiae, Candida albicans, and on dormant and germinating spores of Aspergillus niger

Five new dimer compounds, namely Taiwaniacryptodimers A‒E (1–5), were isolated from the methanol extract of the roots of Taiwania cryptomerioides. Their structures were established by mean of spectroscopic analysis and comparison of NMR data with those of known analogues. Their antifungal activities were also evaluated. Our results indicated that metabolites 1, 2, 4, and 5 displayed moderate antifungal activities against Aspergillus niger, Penicillium italicum, Candida albicans, and Saccharomyces cerevisiae.

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KHẢO SÁT HOẠT TÍNH KHÁNG VI SINH VẬT KIỂM ĐỊNH CỦA LOÀI KÉ HOA ĐÀO (URENA LOBATA L.)

SCIENTIFIC JOURNAL OF TAN TRAO UNIVERSITY ◽

10.51453/2354-1431/2021/587 ◽

2021 ◽

Vol 7 (21) ◽

pp. 183-189

Author(s):

Thu Hương Trương Thị ◽

Hoài Vân Trần Thị ◽

Thùy Dương Trần Thị ◽

Mai Chu Quỳnh ◽

Nguyệt Nguyễn Thị

Keyword(s):

Escherichia Coli ◽

Saccharomyces Cerevisiae ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Aspergillus Niger ◽

Ethyl Acetate ◽

Fusarium Oxysporum

Các cặn chiết ethanol tổng, n-hexane, ethyl acetate và 2 hợp chất 5-hydroxy-6,7-dimetoxyflavanon và 3β-hydroxy-urs-12-en-28-oic phân lập được từ loài Ké hoa đào (Urena lobata) thể hiện hoạt tính kháng tốt đối với 2 chủng vi khuẩn Gram (–) (Escherichia coli và Pseudomonas aeruginosa), 2 chủng Gram (+) (Bacillus subtillis và Staphylococcus aureus), 02 chủng nấm sợi (Aspergillus niger và Fusarium oxysporum), 2 chủng nấm men (Candida albicans và Saccharomyces cerevisiae) với đường kính vòng kháng khuẩn từ 8 đến 28 mm.

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Synthesis, characterization and antifungal testing of 3,4-dihydropyrimidin-2(1H)-(thio)ones containing boronic acids and boronate esters

Open Chemistry ◽

10.2478/s11532-008-0064-x ◽

2008 ◽

Vol 6 (4) ◽

pp. 562-568 ◽

Cited By ~ 5

Author(s):

Dominique Duguay ◽

Matthew Zamora ◽

Johanna Blacquiere ◽

Francis Appoh ◽

Christopher Vogels ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Candida Albicans ◽

Antifungal Activity ◽

Aspergillus Niger ◽

Acid Catalyst ◽

Boronic Acids ◽

Boronate Esters ◽

Pure Cultures ◽

Lewis Acid Catalyst ◽

Moderate Yield

AbstractThe addition of formylphenylboronic acid derivatives to thiourea and ethyl acetoacetate proceeds in the presence of an additional Lewis acid catalyst to give the corresponding 3,4-dihydropyrimidin-2(1H)-(thio)ones (Biginelli products) in moderate yield. Compounds were tested for antifungal activity against pure cultures of Aspergillus niger, Aspergillus flavus, Candida albicans and Saccharomyces cerevisiae but, unfortunately, none showed any appreciable activity.

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Atividade antimicrobiana in vitro do rizoma em pó, dos pigmentos curcuminóides e dos óleos e dos essenciais da Curcuma longa L.

Ciência e Agrotecnologia ◽

10.1590/s1413-70542008000300026 ◽

2008 ◽

Vol 32 (3) ◽

pp. 875-881 ◽

Cited By ~ 13

Author(s):

Lúcia Péret-Almeida ◽

Cristina da Cunha Naghetini ◽

Elzíria de Aguiar Nunan ◽

Roberto Gonçalves Junqueira ◽

Maria Beatriz Abreu Glória

Keyword(s):

Escherichia Coli ◽

Saccharomyces Cerevisiae ◽

Staphylococcus Aureus ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Aspergillus Niger ◽

Curcuma Longa ◽

E Coli ◽

Salmonella Choleraesuis

Este trabalho teve como objetivo avaliar a atividade antimicrobiana da cúrcuma em pó, da curcumina disponível no comércio, dos pigmentos curcuminóides purificados e dos óleos essenciais da cúrcuma. As amostras foram analisadas quanto aos teores de pigmentos curcuminóides por cromatografia líquida de alta eficiência. O óleo essencial foi também analisado quanto à densidade, índice de refração e perfil das substâncias voláteis por cromatografia gasosa e detector de ionização de chamas. A atividade antimicrobiana in vitro foi determinada pelo método de difusão em ágar com discos de papel estéreis, impregnados com os extratos, sendo os diâmetros dos halos de inibição medidos com paquímetro. Os extratos etanólicos da cúrcuma, da curcumina comercial, e dos pigmentos curcumina e desmetoxicurcumina não inibiram o crescimento de Staphylococcus aureus, Bacillus subtilis, Salmonella choleraesuis, Escherichia coli, Aspergillus niger, Saccharomyces cerevisiae, e Candida albicans. Apenas o extrato etanólico da bisdesmetoxicurcumina apresentou atividade antimicrobiana em relação ao B. subtilis. O óleo essencial da cúrcuma apresentou atividade antimicrobiana para o B. subtilis, S. choleraesuis, E. coli, A. niger e S. cerevisiae, sendo que essa atividade aumentou em função do aumento da concentração. Os halos de inibição, obtidos com o óleo essencial, foram significativos, quando comparados aos respectivos antibióticos tradicionais, cloranfenicol e anfotericina, indicando ser o óleo essencial da cúrcuma um agente antimicrobiano em potencial.

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ANTIMICROBIAL ACTIVITY OF HYMENAEA COURBARIL L. FRUIT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i6.37272 ◽

2020 ◽

pp. 200-203

Author(s):

MARIANA NARVAEZ CORREA ◽

OSCAR EDUARDO RODRÍGUEZ AGUIRRE ◽

JANETH DEL CARMEN ARIAS PALACIOS

Keyword(s):

Escherichia Coli ◽

Saccharomyces Cerevisiae ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Aspergillus Niger ◽

Hymenaea Courbaril ◽

Test Conditions

Objective: The assessment of the antimicrobial activity of Hymenaea courbaril L. on different microorganisms was realized with four bacteria, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa, two yeasts Candida albicans and Saccharomyces cerevisiae, and finally two filamentous fungi Aspergillus niger. Methods: The method of plates and wells was used, using extracts from the fruit of the plant mentioned above. These extracts were made with different solvents such as hexane, dichloromethane, ethanol, and aqueous. Results: It was determined that the dichloromethane extract of H. courbaril L. has antimicrobial activity against the bacterium S. aureus showing a percentage of inhibition of 1.47%. Conclusions: In comparison to bacteria, fungi do not represent a significant inhibitory capacity which represents that when comparing these extracts of this plant, under the test conditions evaluated, it was presented that they obtained antimicrobial activity against S. aureus.

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Potent Chitin Synthase Inhibitors from Plants

Current Bioactive Compounds ◽

10.2174/1573407213666180719145831 ◽

2020 ◽

Vol 16 (1) ◽

pp. 58-63

Author(s):

Amrutha Vijayakumar ◽

Ajith Madhavan ◽

Chinchu Bose ◽

Pandurangan Nanjan ◽

Sindhu S. Kokkal ◽

...

Keyword(s):

Cell Wall ◽

Candida Albicans ◽

Caenorhabditis Elegans ◽

Aspergillus Niger ◽

Small Molecules ◽

Tip Growth ◽

Chitin Synthase ◽

Chitin Synthesis ◽

Hyphal Tip Growth ◽

Hyphal Tip

Background: Chitin is the main component of fungal, protozoan and helminth cell wall. They help to maintain the structural and functional characteristics of these organisms. The chitin wall is dynamic and is repaired, rearranged and synthesized as the cells develop. Active synthesis can be noticed during cytokinesis, laying of primary septum, maintenance of lateral cell wall integrity and hyphal tip growth. Chitin synthesis involves coordinated action of two enzymes namely, chitin synthase (that lays new cell wall) and chitinase (that removes the older ones). Since chitin synthase is conserved in different eukaryotic microorganisms that can be a ‘soft target’ for inhibition with small molecules. When chitin synthase is inhibited, it leads to the loss of viability of cells owing to the self- disruption of the cell wall by existing chitinase. Methods: In the described study, small molecules from plant sources were screened for their ability to interfere with hyphal tip growth, by employing Hyphal Tip Burst assay (HTB). Aspergillus niger was used as the model organism. The specific role of these small molecules in interfering with chitin synthesis was established with an in-vitro method. The enzyme required was isolated from Aspergillus niger and its activity was deduced through a novel method involving non-radioactively labelled substrate. The activity of the potential lead molecules were also checked against Candida albicans and Caenorhabditis elegans. The latter was adopted as a surrogate for the pathogenic helminths as it shares similarity with regard to cell wall structure and biochemistry. Moreover, it is widely studied and the methodologies are well established. Results: Out of the 11 compounds and extracts screened, 8 were found to be prospective. They were also found to be effective against Candida albicans and Caenorhabditis elegans. Conclusion: Purified Methyl Ethyl Ketone (MEK) Fraction1 (F1) of Coconut (Cocos nucifera) Shell Extract (COSE) was found to be more effective against Candida albicans with an IC50 value of 3.04 μg/mL and on L4 stage of Caenorhabditis elegans with an IC50 of 77.8 μg/mL.

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A Novel Antimicrobial Phenanthrene Alkaloid from Bryopyllum pinnatum

E-Journal of Chemistry ◽

10.1155/2011/972359 ◽

2011 ◽

Vol 8 (3) ◽

pp. 1456-1461 ◽

Cited By ~ 2

Author(s):

Donatus Ebere Okwu ◽

Fred Uchenna Nnamdi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Candida Albicans ◽

Aspergillus Niger ◽

Ethanolic Extract ◽

Klebsiella Pneumonia ◽

Antimicrobial Studies ◽

Prevention And Treatment ◽

Kalanchoe Pinnata ◽

Isolated Compound

Phenenthrene alkaloid identified as 1-ethanamino 7 hex-1-yne-5I-one phenanthrene was isolated from the ethanolic extract of the leaves ofBryophyllum pinnatum(syn. B. calcinum kalanchoe pinnata) a versatile Nigeria medicinal plant. The structure was elucidated using NMR, IR, UV and MS spectral data. Antimicrobial studies showed that the isolated compound successfully inhibitedPsuedomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Escherichia coli, Candida albicansandAspergillus niger. This result authenticates the use ofbryophyllum pinantumin phytomedicine for disease prevention and treatment of infections.

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Divergent Subunit Interactions among Fungal mRNA 5′-Capping Machineries

Eukaryotic Cell ◽

10.1128/ec.1.3.448-457.2002 ◽

2002 ◽

Vol 1 (3) ◽

pp. 448-457 ◽

Cited By ~ 17

Author(s):

Toshimitsu Takagi ◽

Eun-Jung Cho ◽

Rozmin T. K. Janoo ◽

Vladimir Polodny ◽

Yasutaka Takase ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Candida Albicans ◽

Rna Polymerase Ii ◽

Subunit Interactions ◽

Pol Ii ◽

Mrna Capping ◽

Capping Enzyme ◽

Enzyme Subunits ◽

Carboxyl Terminal

ABSTRACT The Saccharomyces cerevisiae mRNA capping enzyme consists of two subunits: an RNA 5′-triphosphatase (RTPase) and GTP::mRNA guanylyltransferase (GTase). The GTase subunit (Ceg1) binds to the phosphorylated carboxyl-terminal domain of the largest subunit (CTD-P) of RNA polymerase II (pol II), coupling capping with transcription. Ceg1 bound to the CTD-P is inactive unless allosterically activated by interaction with the RTPase subunit (Cet1). For purposes of comparison, we characterize here the related GTases and RTPases from the yeasts Schizosaccharomyces pombe and Candida albicans. Surprisingly, the S. pombe capping enzyme subunits do not interact with each other. Both can independently interact with CTD-P of pol II, and the GTase is not repressed by CTD-P binding. The S. pombe RTPase gene (pct1 +) is essential for viability. Pct1 can replace the S. cerevisiae RTPase when GTase activity is supplied by the S. pombe or mouse enzymes but not by the S. cerevisiae GTase. The C. albicans capping enzyme subunits do interact with each other. However, this interaction is not essential in vivo. Our results reveal an unexpected diversity among the fungal capping machineries.

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