Fucoidan refined by Sargassum confusum indicate protective effects suppressing photo-oxidative stress and skin barrier perturbation in UVB-induced human keratinocytes

AbstractThe present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.

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Protective effects of resveratrol against 5-fluorouracil-induced oxidative stress and inflammatory responses in human keratinocytes

Journal of Clinical Biochemistry and Nutrition ◽

10.3164/jcbn.21-23 ◽

2021 ◽

Author(s):

Shu Chen ◽

Naofumi Tamaki ◽

Yasusei Kudo ◽

Takaaki Tsunematsu ◽

Kaname Miki ◽

...

Keyword(s):

Oxidative Stress ◽

Inflammatory Responses ◽

Human Keratinocytes ◽

Protective Effects

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Protective effect of kudzu root vinegar and adenosine against UVB-induced oxidative stress in human keratinocytes

SDRP Journal of Cellular and Molecular Physiology ◽

10.25177/jcmp.3.2.ra.10673 ◽

2020 ◽

Vol 3 (2) ◽

pp. 184-192

Author(s):

Ji Eun Park ◽

◽

Young Mi Kim

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Hacat Cell ◽

Reactive Oxygen ◽

Human Keratinocytes ◽

Mitogen Activated Protein Kinase ◽

Hacat Cells ◽

Oxygen Species ◽

Protective Effects ◽

Skin Damage

Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in cells, which induces sunburn cell formation, melanoma, photoaging, and skin cancer. This study examines the anti-photodamage effects of kudzu root vinegar and adenosine in UVB-exposed human keratinocytes (HaCaT cells). UVB significantly decreased HaCaT cell viability, whereas kudzu root vinegar and adenosine did not exhibit cytotoxic effects and increased the viability of HaCaT cells. To investigate the protective effects of kudzu root vinegar and adenosine on UVB-induced oxidative stress in HaCaT cells, ROS, matrix metalloproteinases (MMPs), and mitogen-activated protein kinase (MAPK) were analyzed. UVB-induced treatment reduced the activity of antioxidant enzymes; however, kudzu root vinegar and adenosine increased their activity. These results indicated that kudzu root vinegar and adenosine exert cytoprotective activity against UVB-induced oxidative stress in HaCaT cells. Moreover, they suppressed the UVB-induced downregulation of MMPs and inhibited the phosphorylation of MAPK induced by UVB-irradiation. Therefore, kudzu root vinegar and adenosine offer anti-oxidative effects, via lowering ROS production, suppressing JNK activation, and downregulating expression of MMPs. Our findings suggest that kudzu root vinegar and adenosine have potential application in preventing skin damage owing to UVB exposure. Keywords: reactive oxygen species (ROS), HaCaT cell, UVB, skin damage, anti-aging

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Protective Effect of Phloroglucinol on Oxidative Stress-Induced DNA Damage and Apoptosis through Activation of the Nrf2/HO-1 Signaling Pathway in HaCaT Human Keratinocytes

Marine Drugs ◽

10.3390/md17040225 ◽

2019 ◽

Vol 17 (4) ◽

pp. 225 ◽

Cited By ~ 16

Author(s):

Cheol Park ◽

Hee-Jae Cha ◽

Su Hyun Hong ◽

Gi-Young Kim ◽

Suhkmann Kim ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Signaling Pathway ◽

Protective Efficacy ◽

Human Keratinocytes ◽

Heme Oxygenase 1 ◽

Zinc Protoporphyrin ◽

Related Factor ◽

Protective Effects ◽

Skin Keratinocytes

Phloroglucinol (PG) is a component of phlorotannins, which are abundant in marine brown alga species. Recent studies have shown that PG is beneficial in protecting cells from oxidative stress. In this study, we evaluated the protective efficacy of PG in HaCaT human skin keratinocytes stimulated with oxidative stress (hydrogen peroxide, H2O2). The results showed that PG significantly inhibited the H2O2-induced growth inhibition in HaCaT cells, which was associated with increased expression of heme oxygenase-1 (HO-1) by the activation of nuclear factor erythroid 2-related factor-2 (Nrf2). PG remarkably reversed H2O2-induced excessive ROS production, DNA damage, and apoptosis. Additionally, H2O2-induced mitochondrial dysfunction was related to a decrease in ATP levels, and in the presence of PG, these changes were significantly impaired. Furthermore, the increases of cytosolic release of cytochrome c and ratio of Bax to Bcl-2, and the activation of caspase-9 and caspase-3 by the H2O2 were markedly abolished under the condition of PG pretreatment. However, the inhibition of HO-1 function using zinc protoporphyrin, a HO-1 inhibitor, markedly attenuated these protective effects of PG against H2O2. Overall, our results suggest that PG is able to protect HaCaT keratinocytes against oxidative stress-induced DNA damage and apoptosis through activating the Nrf2/HO-1 signaling pathway.

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Aster glehniExtract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPARδ-AMPK Pathway

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/9616574 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13

Author(s):

Yong-Jik Lee ◽

Yoo-Na Jang ◽

Yoon-Mi Han ◽

Hyun-Min Kim ◽

Changbae Jin ◽

...

Keyword(s):

Transient Receptor Potential ◽

Sodium Dodecyl ◽

Skin Barrier ◽

Human Keratinocytes ◽

Control Group ◽

Hacat Cells ◽

Protective Effects ◽

Necrosis Factor Alpha ◽

Protein Levels ◽

Ampk Pathway

Aster glehni(AG) has been used in cooking and as a medicine to treat various diseases for over hundreds of years in Korea. To speculate the protective effects of AG on skin barrier, we estimated the protein levels of biomarkers related to skin barrier protection in human keratinocytes, HaCaT cells treated with sodium dodecyl sulfate (SDS), or 2,4-dinitrochlorobenzene (DNCB). The protein levels for keratin, involucrin, defensin, tumor necrosis factor alpha (TNFα), peroxisome proliferator-activated receptor delta (PPARδ), 5′ adenosine monophosphate-activated protein kinase (AMPK), serine palmitoyltransferase long chain base subunit 2 (SPTLC2), and transient receptor potential cation channel subfamily V member 4 (TRPV4) were evaluated using western blotting or immunocytochemistry in HaCaT cells. AG extract increased the protein levels of PPARδ, phosphorylated AMPK, SPTLC2, keratin, involucrin, and defensin compared to the SDS or DNCB control group. However, TNFαexpression increased by SDS or DNCB was decreased with AG extract. The order of action of each regulatory biomarker in AG pathway was identified TRPV4→PPARδ→AMPK from antagonist and siRNA treatment studies. AG can ameliorate the injury of keratinocytes caused by SDS or DNCB through the sequential regulation of TRPV4→PPARδ→AMPK pathway.

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Protective Effects of Cyanidin-3-O-β-glucopyranoside Against UVA-induced Oxidative Stress in Human Keratinocytes¶

Photochemistry and Photobiology ◽

10.1111/j.1751-1097.2005.tb00235.x ◽

2007 ◽

Vol 81 (3) ◽

pp. 623-629 ◽

Cited By ~ 2

Author(s):

Andrea Tarozzi ◽

Alessandra Marchesl ◽

Silvana Hrelia ◽

Cristina Angeloni ◽

Vincenza Andrisano ◽

...

Keyword(s):

Oxidative Stress ◽

Human Keratinocytes ◽

Protective Effects

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Glycofullerenes Inhibit Particulate Matter Induced Inflammation and Loss of Barrier Proteins in HaCaT Human Keratinocytes

Biomolecules ◽

10.3390/biom10040514 ◽

2020 ◽

Vol 10 (4) ◽

pp. 514

Author(s):

Chiang-Wen Lee ◽

Yu-Han Su ◽

Yao-Chang Chiang ◽

I-Ta Lee ◽

Sin-Yu Li ◽

...

Keyword(s):

Particulate Matter ◽

Water Solubility ◽

Skin Diseases ◽

Skin Barrier ◽

Human Keratinocytes ◽

Mitogen Activated Protein Kinase ◽

Radical Scavenger ◽

Heme Oxygenase 1 ◽

Protective Effects ◽

Ros Scavenging

Exposure to particulate matter (PM) has been linked to pulmonary and cardiovascular dysfunctions, as well as skin diseases, etc. PM impairs the skin barrier functions and is also involved in the initiation or exacerbation of skin inflammation, which is linked to the activation of reactive oxygen species (ROS) pathways. Fullerene is a single C60 molecule which has been reported to act as a good radical scavenger. However, its poor water solubility limits its biological applications. The glyco-modification of fullerenes increases their water solubility and anti-bacterial and anti-virus functions. However, it is still unclear whether it affects their anti-inflammatory function against PM-induced skin diseases. Hence, glycofullerenes were synthesized to investigate their effects on PM-exposed HaCaT human keratinocytes. Our results showed that glycofullerenes could reduce the rate of PM-induced apoptosis and ROS production, as well as decrease the expression of downstream mitogen-activated protein kinase and Akt pathways. Moreover, PM-induced increases in inflammatory-related signals, such as cyclooxygenase-2, heme oxygenase-1, and prostaglandin E2, were also suppressed by glycofullerenes. Notably, our results suggested that PM-induced impairment of skin barrier proteins, such as filaggrin, involucrin, repetin, and loricrin, could be reduced by pre-treatment with glycofullerenes. The results of this study indicate that glycofullerenes could be potential candidates for treatments against PM-induced skin diseases and that they exert their protective effects via ROS scavenging, anti-inflammation, and maintenance of the expression of barrier proteins.

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(−)-Loliolide Isolated from Sargassum horneri Protects against Fine Dust-Induced Oxidative Stress in Human Keratinocytes

Antioxidants ◽

10.3390/antiox9060474 ◽

2020 ◽

Vol 9 (6) ◽

pp. 474

Author(s):

Mawalle Kankanamge Hasitha Madhawa Dias ◽

Dissanayaka Mudiyanselage Dinesh Madusanka ◽

Eui Jeong Han ◽

Min Ju Kim ◽

You-Jin Jeon ◽

...

Keyword(s):

Oxidative Stress ◽

Nuclear Translocation ◽

Human Keratinocytes ◽

Sargassum Horneri ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Protective Effects ◽

Fine Dust ◽

Apoptosis Pathway ◽

Sustainable Solutions

The emergence of fine dust (FD) among air pollutants has taken a toll during the past few decades, and it has provided both controversy and a platform for open conversation amongst world powers for finding sustainable solutions and effective treatments for health issues. The present study emphasizes the protective effects of (–)-loliolide (HTT) isolated from Sargassum horneri against FD-induced oxidative stress in human HaCaT keratinocytes. The purification of (–)-loliolide was carried out by centrifugal partition chromatography. HTT did not show any cytotoxicity, and it further illustrated the potential to increase cell viability by reducing the reactive oxygen species (ROS) production in FD-stimulated keratinocytes. Furthermore, HTT suppressed FD-stimulated DNA damage and the formation of apoptotic bodies, and it reduced the population of cells in the sub-G1 apoptosis phase. FD-induced apoptosis was advancing through the mitochondria-mediated apoptosis pathway. The cytoprotective effects of the HTT against FD-stimulated oxidative damage is mediated through squaring the nuclear factor E2-related factor 2 (Nrf2)-mediated heme oxygenase-1 (HO-1) pathway, dose-dependently increasing HO-1 and NAD(P)H dehydrogenase (quinone) 1 (NQO1) levels in the cytosol while concomitantly improving the nuclear translocation of Nrf2. Future studies could implement the protective functionality of HTT in producing pharmaceuticals that utilize natural products and benefit the diseased.

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