Characterization of a transferable bcrABC and cadAC genes-harboring plasmid in Listeria monocytogenes strain isolated from food products of animal origin

2016 ◽  
Vol 217 ◽  
pp. 117-122 ◽  
Author(s):  
Dongyang Xu ◽  
Qing Nie ◽  
Wenyan Wang ◽  
Lei Shi ◽  
He Yan
Keyword(s):  
Listeria Monocytogenes ◽  
Food Products ◽  
Animal Origin

scholarly journals Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco

Food Control ◽  
2018 ◽  
Vol 84 ◽  
pp. 436-441 ◽  
Author(s):  
Nadia Amajoud ◽  
Alexandre Leclercq ◽  
Jose M. Soriano ◽  
Hélène Bracq-Dieye ◽  
Mohammed El Maadoudi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Products

scholarly journals Novel Sequence Types of Listeria monocytogenes of Different Origin Obtained in the Republic of Serbia

2021 ◽  
Vol 9 (6) ◽  
pp. 1289
Author(s):  
Tatiana Yu. Bespalova ◽  
Tatiana V. Mikhaleva ◽  
Nadezhda Yu Meshcheryakova ◽  
Olga V. Kustikova ◽  
Kazimir Matovic ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Relatedness ◽  
Small Ruminants ◽  
Food Products ◽  
Animal Origin ◽  
Mammal Species ◽  
Food Borne ◽  
The Republic ◽  
Phylogenetic Lineages ◽  
Sequence Types

Listeria monocytogenes, the causative agent of listeriosis, is amongst the major food-borne pathogens in the world that affect mammal species, including humans. This microorganism has been associated with both sporadic episodes and large outbreaks of human listeriosis worldwide, with high mortality rates. In this study, the main sequence types (STs) and clonal complexes (CCs) were investigated in all of the 13 L. monocytogenes strains originating from different sources in the Republic of Serbia in 2004–2019 and that were available in the BIGSdb-Lm database. We found at least 13 STs belonging to the phylogenetic lineages I and II. These strains were represented by ST1/ST3/ST9 of CC1/CC3/CC9, which were common in the majority of the European countries and worldwide, as well as by eight novel STs (ST1232/ST1233/ST1234/ST1235/ST1238/ST1236/ST1237/ST1242) of CC19/CC155/CC5/CC21/CC3/CC315/CC37, and the rare ST32 (clonal complex ST32) and ST734 (CC1), reported in the Republic of Serbia, the EU, for the first time. Our study confirmed the association of CC1 with cases of neuroinfection and abortions among small ruminants, and of CC3 and CC9 with food products of animal origin. The strains isolated in 2019 carried alleles of the internalin genes (inlA/inlB/inlC/inlE) characteristic of the most virulent strains from the hypervirulent CC1. These findings demonstrated the genetic relatedness between L. monocytogenes strains isolated in the Republic of Serbia and worldwide. Our study adds further information about the diversity of the L. monocytogenes genotypes of small ruminants and food products, as the strain distribution in these sources in Serbia had not previously been evaluated.

scholarly journals Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1381
Author(s):  
Eman E. Abdeen ◽  
Walid S. Mousa ◽  
Ola. H. Harb ◽  
Gehad A. Fath-Elbab ◽  
Mohammed Nooruzzaman ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Characterization ◽  
Food Products ◽  
Raw Milk ◽  
Food Samples ◽  
World Health ◽  
Fish Fillet ◽  
Minced Meat ◽  
The Impact

World Health Organization classified Listeria monocytogenes as a major notable foodborne pathogen associated with high mortality and hospitalization. The study reports the prevalence, antibiogram, virulence determination and genetic characterization of L. monocytogenes from different food products. A total of 250 food samples, fifty samples each from raw milk, ice cream, minced meat, fish fillet and sausage were collected from the Menoufiya governorate in Egypt. L. monocytogenes was detected in 17 (6.8%) of the tested food samples including minced meat (14%), fish fillet (8%), sausage (6%) and raw milk (6%). The antimicrobial susceptibility assay of 17 L. monocytogenes isolates against seventeen antibiotics belonging to eight antibiotics classes revealed a high susceptibility to norfloxacin (82.3%), amoxicillin-clavulanic acid (76.4%), cefotaxime (70.5%), erythromycin (64.6%), amoxicillin (64.6%), gentamicin (58.7%) and vancomycin (58.7%). While, high resistance was observed against oxytetracycline (76.4%), trimethoprim-sulfamethoxazole (76.4%), chloramphenicol (70.5%), doxycycline (64.6%), levofloxacin (41.2%) and azithromycin (41.2%). Of note, all L. monocytogenes isolates were multidrug-resistant. The multiplex PCR successfully amplified L. monocytogenes in all tested isolates. Screening of the five virulence-related genes revealed the hlyA and iap as the most prevalent genes followed by actA gene, however, the inlA and prfA genes were not detected in any of the studied isolates. The partial 16S rRNA gene sequencing of three L. monocytogenes isolates showed a high nucleotide similarity (99.1–99.8%) between the study isolates and various global clones, and phylogenetic analysis clustered these L. monocytogenes strains with other Listeria species including L. welshimeri, L. seeligeri and L. innocua. This study demonstrates the impact of L. monocytogenes as a major contaminant of various food products and suggests more attention to the awareness and hygienic measures in the food industry.

scholarly journals Diversity and antibiotic resistance profiles of Listeria monocytogenes serogroups in different food products from Transylvania Region, Central Romania

2021 ◽  
Author(s):  
Emil Tirziu ◽  
Viorel Herman ◽  
Ileana Nichita ◽  
Adriana Morar ◽  
Mirela Imre ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Acid Resistance ◽  
Food Products ◽  
The Other ◽  
Animal Origin ◽  
Public Health Issue ◽  
Raw Meat ◽  
Isolation Frequency ◽  
Antimicrobial Susceptibility Profile ◽  
Animal Origin Food

The aim of this study was to assess the presence and antimicrobial susceptibility profile of the molecularly serogrouped Listeria monocytogenes isolates in different animal origin food products, collected from a county situated in the historical region of Transylvania, Central Romania. A total of 7.7% (17/221) of the screened samples were positive for L. monocytogenes , with an isolation frequency of 6.2% (8/130) in the ready-to-eat products (i.e., sausages, ham and smoked specialties), 12.8% (6/47) in raw meat (i.e., minced pork, pork organs and snails), and 6.8% (3/44) in dairy (i.e., assortment of cheeses) samples. The identified L. monocytogenes serogroups were: 1/2a-3a (47.1%), 4b-4d-4e (29.4%), 1/2c-3c (11.8%), and 4a-4c (11.8%), respectively. All isolates were resistant to benzylpenicillin and fusidic acid. Resistance was also detected towards oxacillin (88.2%), fosfomycin (82.4%), clindamycin (76.5%), imipenem (52.9%), ciprofloxacin (41.2%), rifampicin (41.2%), trimethoprim – sulfamethoxazole (29.4%) and tetracycline (29.4%). On the other hand, all isolates proved susceptible to gentamicin, moxifloxacin, teicoplanin, vancomycin, tigecycline, erythromycin and linezolid. All tested strains exhibited multidrug resistance, resulting in the expression of a total of 12 resistance profiles. These findings extend the understanding about the spread of an important pathogen in Romanian food products, highlighting a substantial public health issue and medical concern, especially for consumers with a compromised health status.

scholarly journals Prevalence and toxicity characterization of Bacillus cereus in food products from Poland

Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 269 ◽  
Author(s):  
Berthold-Pluta ◽  
Pluta ◽  
Garbowska ◽  
Stefańska
Keyword(s):  
Bacillus Cereus ◽  
Food Products ◽  
Animal Origin ◽  
Infant Formulas ◽  
Pasteurized Milk ◽  
Ferment Lactose ◽  
Favorable Conditions ◽  
Foodborne Infections ◽  
Hydrolyze Casein

The prevalence of Bacillus cereus in a total of 585 samples of food products (herbs and spices, breakfast cereals, pasta, rice, infant formulas, pasteurized milk, fresh acid and acid/rennet cheeses, mold cheeses and ripening rennet cheeses) marketed in Poland was investigated. The potential of 1022 selected isolates of B. cereus to hydrolyze casein, starch and tributyrin, to ferment lactose, to grow at 7 C/10 days, to produce Nhe and Hbl toxin and to possess the ces gene was verified. B. cereus was found in 38.8% of the analyzed samples, reaching levels from 0.3 to 3.8 log CFU g-1 or mL-1. From the 1022 isolates, 48.8%, 36.0%, 98.9%, 80.0% and 25.0% were capable of fermenting lactose, producing amylase, protease, lipase and growing at 7 C/10 days, respectively, indicating spoilage potentiality. The occurrence of toxigenic B. cereus strains in all tested market products, both of plant (55.8% Hbl(+), 70.7% Nhe(+) and 1.7% ces(+) isolates) and animal origin (84.9% Hbl(+), 82.7% Nhe(+) and 0.9% ces(+) isolates) indicates the possible risk of foodborne infections/intoxications that occur as a result of the possibility of the development of B. cereus in favorable conditions and consumption of these products.

scholarly journals PREVALENCE AND CHARACTERIZATION OF LISTERIA MONOCYTOGENES ISOLATED FROM DIFFERENT FOOD PRODUCTS

2011 ◽  
Vol 1 (1zero) ◽  
pp. 273
Author(s):  
A. Corda ◽  
M. P. Cogoni ◽  
R. Sabiu ◽  
S. Brignardello ◽  
A. Parisi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Products

scholarly journals Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259687
Author(s):  
Emilia Fernanda Agostinho Davanzo ◽  
Rebecca Lavarini dos Santos ◽  
Virgilio Hipólito de Lemos Castro ◽  
Joana Marchesini Palma ◽  
Bruno Rocha Pribul ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Foodborne Pathogens ◽  
Federal District ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Meat Production ◽  
Salmonella Spp

Listeria monocytogenes and Salmonella spp. are considered important foodborne pathogens that are commonly associated with foods of animal origin. The aim of this study was to perform molecular characterization of L. monocytogenes and Salmonella spp. isolated from biofilms of cattle and poultry slaughterhouses located in the Federal District and State of Goiás, Brazil. Fourteen L. monocytogenes isolates and one Salmonella sp. were detected in poultry slaughterhouses. No isolates were detected in cattle slaughterhouses. All L. monocytogenes isolates belonged to lineage II, and 11 different pulsotypes were detected. Pulsed-field gel electrophoresis analysis revealed the dissemination of two strains within one plant, in addition to the regional dissemination of one of them. The Salmonella isolate was identified via whole genome sequencing as Salmonella enterica serovar Minnesota ST548. In the sequence analysis, no premature stop codons were detected in the inlA gene of Listeria. All isolates demonstrated the ability to adhere to Caco-2 cells, while 50% were capable of invading them. Antimicrobial resistance was detected in 57.1% of the L. monocytogenes isolates, and resistance to sulfonamide was the most common feature. The tetC, ermB, and tetM genes were detected, and four isolates were classified as multidrug-resistant. Salmonella sp. was resistant to nine antimicrobials and was classified as multidrug-resistant. Resistance genes qnrB19, blaCMY-2, aac(6’)-Iaa, sul2, and tetA, and a mutation in the parC gene were detected. The majority (78.5%) of the L. monocytogenes isolates were capable of forming biofilms after incubation at 37°C for 24 h, and 64.3% were capable of forming biofilms after incubation at 12°C for 168 h. There was no statistical difference in the biofilm-forming capacity under the different evaluated conditions. Salmonella sp. was capable of forming biofilms at both tested temperatures. Biofilm characterization was confirmed by collecting the samples consistently, at the same sampling points, and by assessing biofilm formation in vitro. These results highlight the potential risk of cross-contamination in poultry slaughterhouses and the importance of surveillance and pathogen control maintenance programs within the meat production industry.

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