Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil

Listeria monocytogenes and Salmonella spp. are considered important foodborne pathogens that are commonly associated with foods of animal origin. The aim of this study was to perform molecular characterization of L. monocytogenes and Salmonella spp. isolated from biofilms of cattle and poultry slaughterhouses located in the Federal District and State of Goiás, Brazil. Fourteen L. monocytogenes isolates and one Salmonella sp. were detected in poultry slaughterhouses. No isolates were detected in cattle slaughterhouses. All L. monocytogenes isolates belonged to lineage II, and 11 different pulsotypes were detected. Pulsed-field gel electrophoresis analysis revealed the dissemination of two strains within one plant, in addition to the regional dissemination of one of them. The Salmonella isolate was identified via whole genome sequencing as Salmonella enterica serovar Minnesota ST548. In the sequence analysis, no premature stop codons were detected in the inlA gene of Listeria. All isolates demonstrated the ability to adhere to Caco-2 cells, while 50% were capable of invading them. Antimicrobial resistance was detected in 57.1% of the L. monocytogenes isolates, and resistance to sulfonamide was the most common feature. The tetC, ermB, and tetM genes were detected, and four isolates were classified as multidrug-resistant. Salmonella sp. was resistant to nine antimicrobials and was classified as multidrug-resistant. Resistance genes qnrB19, blaCMY-2, aac(6’)-Iaa, sul2, and tetA, and a mutation in the parC gene were detected. The majority (78.5%) of the L. monocytogenes isolates were capable of forming biofilms after incubation at 37°C for 24 h, and 64.3% were capable of forming biofilms after incubation at 12°C for 168 h. There was no statistical difference in the biofilm-forming capacity under the different evaluated conditions. Salmonella sp. was capable of forming biofilms at both tested temperatures. Biofilm characterization was confirmed by collecting the samples consistently, at the same sampling points, and by assessing biofilm formation in vitro. These results highlight the potential risk of cross-contamination in poultry slaughterhouses and the importance of surveillance and pathogen control maintenance programs within the meat production industry.

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Molecular Characterization of Listeria Monocytogenes Isolated from Different Animal-Origin Food Items from Urban and Rural Areas

Advances in Animal and Veterinary Sciences ◽

10.17582/journal.aavs/2019/7.s2.51.56 ◽

2019 ◽

Vol 7 (2s) ◽

Author(s):

Azza S. El-Demerdash ◽

Mona T. Raslan

Keyword(s):

Listeria Monocytogenes ◽

Molecular Characterization ◽

Rural Areas ◽

Animal Origin ◽

Food Items ◽

Urban And Rural Areas ◽

Animal Origin Food

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Presence of Antibiotic-Resistant Pathogens in School Cafeteria’s Fast Foods in Dhaka City, Bangladesh: A Growing Concern

International Journal of Pathogen Research ◽

10.9734/ijpr/2021/v7i130171 ◽

2021 ◽

pp. 1-8

Author(s):

Sompa Reza ◽

Ila Ismail ◽

Sharmin Rumi Alim

Keyword(s):

Molecular Characterization ◽

Foodborne Pathogens ◽

Diffusion Method ◽

Rrna Gene ◽

Dhaka City ◽

Biochemical Tests ◽

Salmonella Spp ◽

Fast Foods ◽

Increased Risk

Burden, due to foodborne diseases, particularly Salmonella infection, is high in developing countries like Bangladesh. This research aimed at the molecular characterization of Salmonella spp., isolated from selected school canteen’s fast foods in Dhaka city, Bangladesh, and to evaluate the antibiotic resistance patterns of isolated foodborne pathogens. The school cafeterias were selected by the convenience sampling method. The samples were collected aseptically, and serial dilutions were made. The bacterial colonies were isolated by spread plate technique using appropriate media, and bacterial identification was carried out using gram staining and biochemical tests such as MIU, KIA, Oxidase, and Catalase test. The strain of Salmonella spp. was confirmed by molecular characterization employing the 16S rRNA gene sequencing method. The susceptibility of the isolates to various antibiotics was observed by modified Kirby-Bauer disk diffusion method. Most of the samples were found to contain an unacceptable level of a total aerobic count, which ranged from 5.6×105 to 6.1×107 and 3.4×104 to 7.2×107 for burger and sandwich samples, respectively. Significant isolates from the pathogenic strains were Salmonella spp., Shigella, Klebsiella, Proteus, E. coli, Vibrio spp., Clostridium spp., Staphylococcus spp., and others. The further molecular characterization of isolated Salmonella spp. suggests the similarity with Salmonella enterica serovar Rissen SeqrSC0091. Most isolates were resistant against Ampicillin (100%), Azithromycin (60.87%), Tetracycline (39.43%), Colistin (32.61%), while were highly sensitive to Gentamycin and Chloramphenicol. The presence of multidrug-resistant foodborne pathogens at this high level in the school cafeteria’s fast foods signifies an increased risk for the children’s health.

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Occurrence and phenotypic and molecular characterization of Listeria monocytogenes and Salmonella spp. in slaughterhouses in southern Brazil

Food Research International ◽

10.1016/j.foodres.2017.06.023 ◽

2017 ◽

Vol 100 ◽

pp. 96-101 ◽

Cited By ~ 8

Author(s):

Mariana Almeida Iglesias ◽

Isabela Schneid Kroning ◽

Luana Tombini Decol ◽

Bernadette Dora Gombossy de Melo Franco ◽

Wladimir Padilha da Silva

Keyword(s):

Listeria Monocytogenes ◽

Molecular Characterization ◽

Southern Brazil ◽

Salmonella Spp

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Molecular characterization of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from King Abdulaziz Specialist Hospital at Taif City, Saudi Arabia

Journal of Infection and Public Health ◽

10.1016/j.jiph.2020.12.001 ◽

2021 ◽

Vol 14 (1) ◽

pp. 143-151

Author(s):

Rihab Lagha ◽

Fethi Ben Abdallah ◽

Asmaa A.H. ALKhammash ◽

Nabil Amor ◽

Mohamed M. Hassan ◽

...

Keyword(s):

Saudi Arabia ◽

Klebsiella Pneumoniae ◽

Molecular Characterization ◽

Multidrug Resistant ◽

Clinical Isolates

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The Use of Bacteriophages in the Poultry Industry

Animals ◽

10.3390/ani10050872 ◽

2020 ◽

Vol 10 (5) ◽

pp. 872 ◽

Cited By ~ 6

Author(s):

Katarzyna Żbikowska ◽

Monika Michalczuk ◽

Beata Dolka

Keyword(s):

Foodborne Pathogens ◽

Pathogenic Bacteria ◽

Bacterial Resistance ◽

Health And Safety ◽

Legal Framework ◽

Multidrug Resistant ◽

Poultry Production ◽

Salmonella Spp ◽

Food Contact Surfaces ◽

Public Health And Safety

The emergence of multidrug-resistant infections and antibiotic failures have raised concerns over human and veterinary medicine worldwide. Poultry production has had to confront the problems of an alarming increase in bacterial resistance, including zoonotic pathogens. According to the European Food Safety Authority (EFSA), campylobacteriosis and salmonellosis have been the most frequently reported human foodborne diseases linked to poultry. This situation has strongly stimulated a renewal of scientists’ interest in bacteriophages (phages) since the beginning of the 21st century. Bacteriophages are the viruses of bacteria. They are abundant in nature, and accompany bacteria in each environment they colonize, including human microbiota. In this review, we focused on the use of bacteriophages as therapeutic agents to treat infections and reduce counts of pathogenic bacteria in poultry, as biocontrol agents to eliminate foodborne pathogens on/in food, and also as disinfectants to reduce contamination on food-contact surfaces or poultry carcasses in industrial conditions. Most of the phage-based products are targeted against the main foodborne pathogens, such as Campylobacter jejuni, Salmonella spp., Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Clostridium perfringens. Phages are currently addressed at all stages of the poultry production "from farm to fork", however, their implementation into live birds and food products still provokes discussions especially in the context of the current legal framework, limitations, as well as public health and safety.

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SureSelect targeted enrichment, a new cost effective method for the whole genome sequencing of Candidatus Liberibacter asiaticus

Scientific Reports ◽

10.1038/s41598-019-55144-4 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Weili Cai ◽

Schyler Nunziata ◽

John Rascoe ◽

Michael J. Stulberg

Keyword(s):

Whole Genome Sequencing ◽

Molecular Characterization ◽

Genome Sequencing ◽

Whole Genome Sequence ◽

Whole Genome ◽

Genome Coverage ◽

Metagenomic Sample ◽

Liberibacter Asiaticus

AbstractHuanglongbing (HLB) is a worldwide deadly citrus disease caused by the phloem-limited bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) vectored by Asian citrus psyllids. In order to effectively manage this disease, it is crucial to understand the relationship among the bacterial isolates from different geographical locations. Whole genome sequencing approaches will provide more precise molecular characterization of the diversity among populations. Due to the lack of in vitro culture, obtaining the whole genome sequence of CLas is still a challenge, especially for medium to low titer samples. Hundreds of millions of sequencing reads are needed to get good coverage of CLas from an HLB positive citrus sample. In order to overcome this limitation, we present here a new method, Agilent SureSelect XT HS target enrichment, which can specifically enrich CLas from a metagenomic sample while greatly reducing cost and increasing whole genome coverage of the pathogen. In this study, the CLas genome was successfully sequenced with 99.3% genome coverage and over 72X sequencing coverage from low titer tissue samples (equivalent to 28.52 Cq using Li 16 S qPCR). More importantly, this method also effectively captures regions of diversity in the CLas genome, which provides precise molecular characterization of different strains.

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