Seed and juice characterization of pomegranate fruits grown in Tunisia: Comparison between sour and sweet cultivars revealed interesting properties for prospective industrial applications

: Maltodextrins have an increasing number of biomedical and industrial applications due to their attractive physicochemical properties such as biodegradability and biocompatibility. Herein, we describe the development of a synthetic pathway and characterization of thiol-responsive maltodextrin conjugates with dithiomaleimide linkages. 19F NMR studies were also conducted to demonstrate the exchange dynamics of the dithiomaleimide-functionalized sugar end groups.

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Application of the Continuous Rheoconversion Process (CRP) to Low Temperature HPDC-Part I: Microstructure

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.116-117.402 ◽

2006 ◽

Vol 116-117 ◽

pp. 402-405 ◽

Cited By ~ 4

Author(s):

Qin Yue Pan ◽

Stuart Wiesner ◽

Diran Apelian

Keyword(s):

Low Temperature ◽

Scale Up ◽

Microstructural Characterization ◽

Industrial Applications ◽

Primary Phase ◽

Heat Extraction ◽

Initial Stage ◽

Fraction Solid ◽

Die Castings

The continuous rheoconversion process (CRP) is a novel slurry-on-demand process that was developed at MPI/WPI in 2002. The process is based on a passive liquid mixing technique in which the nucleation and growth of the primary phase are controlled using a specially designed “reactor”. The reactor provides heat extraction, copious nucleation, and forced convection during the initial stage of solidification, thus leading to the formation of globular structures. This paper presents our recent work on the scale-up of the CRP for industrial applications. Specifically, we demonstrate an important application of the CRP to low temperature (low fraction solid) HPDC. In Part I of this paper, we present salient results on microstructural characterization of CRP processed castings vs. conventional die castings.

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Bentonite Clays Characterization in the Town of Sossego – Paraiba State

Materials Science Forum ◽

10.4028/www.scientific.net/msf.820.65 ◽

2015 ◽

Vol 820 ◽

pp. 65-70

Author(s):

I.D.S. Pereira ◽

V.N.F. Lisboa ◽

I.A. Silva ◽

J.M.R. Figueirêdo ◽

G.A. Neves ◽

...

Keyword(s):

Industrial Applications ◽

Particle Size Analysis ◽

Size Analysis ◽

Drilling Fluids ◽

X Ray Diffraction ◽

Mineralogical Characterization ◽

Bentonite Clays ◽

The Town ◽

Petroleum Drilling

Bentonite exhibt a range of industrial applications moving millions of dollars on the market per year. Among several applications if highlights it is use for petroleum drilling fluids. Thus, this work aims to characterize physical, mineralogical and technologically, the bentonitics clays of Sossego County, Paraíba, Brazil. Mineralogical characterization of clays was done through the following techniques: particle size analysis by laser diffraction, thermogravimetric and differential thermal analysis, chemical analysis and x-ray diffraction. The clays were turned in by treatment with sodium in Na2CO3 and then determined whether the apparent viscosity, plastic and volume of filtrate of clay-water dispersions. The results showed that the samples are of polycationic, showing levels of MgO, CaO and K2O, similar to those from Boa Vista County, Paraíba, Brazil, and consist of clay mineral kaolinite, quartz and esmec. The clays showed rheological properties that indicate potential for use in drilling fluids.

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Sortase-mediated site-specific modification of interleukin-2 for the generation of a tumor targeting acetazolamide-cytokine conjugate

10.1101/2020.07.20.211870 ◽

2020 ◽

Author(s):

Baptiste Gouyou ◽

J Millul ◽

A Villa ◽

S Cazzamalli ◽

D Neri ◽

...

Keyword(s):

Carbonic Anhydrase ◽

Tumor Targeting ◽

Interleukin 2 ◽

Industrial Applications ◽

Carbonic Anhydrase Ix ◽

Site Specific ◽

Tumor Bearing ◽

Bioactive Proteins

1.AbstractSmall ligands specific to tumor-associated antigens can be used as alternatives to antibodies for the delivery of small payloads such as radionuclides, cytotoxic drugs and fluorophores. Their use as delivery moiety of bioactive proteins like cytokines remains largely unexplored. Here, we describe the preparation and in vivo characterization of the first small molecule-cytokine conjugate targeting carbonic anhydrase IX (CAIX), a marker of renal cell carcinoma and hypoxia. Site-specific conjugation between interleukin-2 and acetazolamide was obtained by Sortase A-mediated transpeptidation. Binding of the conjugate to the cognate CAIX antigen was confirmed by surface plasmon resonance. The in vivo targeting of structures expressing carbonic anhydrase IX was assessed by biodistribution experiments in tumor bearing mice. Optimization of manufacturability and tumor targeting performance of acetazolamide-cytokine products will be required in order to enable industrial applications.Graphical abstract

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Electrospinning of PCL-Based Blends: Processing Optimization for Their Scalable Production

Materials ◽

10.3390/ma13173853 ◽

2020 ◽

Vol 13 (17) ◽

pp. 3853

Author(s):

Marina P. Arrieta ◽

Adrián Leonés Gil ◽

Maysa Yusef ◽

José M. Kenny ◽

Laura Peponi

Keyword(s):

Thermal Stability ◽

Mechanical Response ◽

Bulk Material ◽

Processing Parameters ◽

Industrial Sector ◽

Industrial Applications ◽

Elongation At Break ◽

Processing Optimization ◽

Scalable Production

In this work poly(ε-caprolactone) (PCL) based electrospun mats were prepared by blending PCL with microcrystalline cellulose (MCC) and poly(3-hydroxybutyrate) (PHB). The electrospinning processing parameters were firstly optimized with the aim to obtain scalable PCL-based electrospun mats to be used in the industrial sector. Neat PCL as well as PCL-MCC and PCL-PHB based mats in different proportions (99:1; 95:5; 90:10) were prepared. A complete morphological, thermal and mechanical characterization of the developed materials was carried out. Scanning electron microscopy (SEM) observations showed that the addition of PHB to the PCL matrix considerably reduced the formation of beads. Both the addition of MCC and PHB reduced the thermal stability of PCL, but obtained materials with enough thermal stability for the intended use. The electrospun PCL fibers show greatly reduced flexibility with respect to the PCL bulk material, however when PCL is blended with PHB their stretchability is increased, changing their elongation at break from 35% to 70% when 10 wt% of PHB is blended with PCL. However, the mechanical response of the different blends increases with respect to the neat electrospun PCL, offering the possibility to modulate their properties according to the required industrial applications.

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Self-repairing mechanism and surface characterization of compressor vanes lubricated with oil added with magnesium silicate hydroxide nanorods

Manufacturing Review ◽

10.1051/mfreview/2019025 ◽

2019 ◽

Vol 6 ◽

pp. 26

Author(s):

Libo Wang ◽

Yi Yang ◽

Yi Qin ◽

Gang Yang ◽

Yuan Qin ◽

...

Keyword(s):

Surface Characterization ◽

Friction And Wear ◽

Magnesium Silicate ◽

Composite Membranes ◽

Great Depth ◽

Industrial Applications ◽

The Self ◽

Great Promise ◽

Surface Modified

Using magnesium silicate hydroxide as additive of lubricating oils for reducing friction in engineering equipment/machinery has been researched intensively. However, some mechanism relating to the growth of the self-repairing layers on the won surfaces is still not clearly explained. At the same time, using magnesium silicate hydroxide (MSH) in the form of nanorods showed great promise in reducing friction and wear. In this study, surface-modified MSH in the form of nanorods was used as additive of polyolester oil (POE) which was then used for the lubrication of compressor vanes. The sample parts were studied on the morphology and the microstructure of the self-repairing layer in a great depth. The results showed that self-repairing layers with different thicknesses were generated on the worn surfaces when the POE with 1 wt.% nanorods-MSH was used. It was found that the self-repairing layers consist of organic–inorganic composite membranes, and with increase of working time of the compressor vanes, the self-repairing layers become denser and thicker, while their micro-structural form remains to be similar. The situ-repairing capability of the metal surfaces (roller-vane pair of the compressor) enforced by the MSH nanorods is very significant, indicating high potential for industrial applications where boundary and mixed lubrications are needed.

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OPTIMIZATION AND CHARACTERIZATION OF EXO-POLYGALACTURONASE BY Aspergillus niger CULTURED VIA SOLID STATE FERMENTATION

Jurnal Teknologi ◽

10.11113/jt.v81.12222 ◽

2018 ◽

Vol 81 (1) ◽

Author(s):

Halifah Pagarra ◽

Roshanida A. Rahman ◽

Nur Izyan Wan Azelee ◽

Rosli Md Illias

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Moisture Content ◽

Solid State Fermentation ◽

Incubation Time ◽

Industrial Applications ◽

Screening Process ◽

Industrial Sectors ◽

Polygalacturonase Production

Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using Nephrolepis biserrata leaves as substrate. Central composite design (CCD) was used to optimize four significant variables resulted from the screening process that has been initially analyzed for the production of exo-polygalacturonase which are incubation time, temperature, concentration of pectin and moisture content. The optimum exo-polygalacturonase production obtained was 54.64 U/g at 120 hours of incubation time, temperature at 340C, 5.0 g/L of pectin concentration and 75.26% of moisture content. For partial characterization of exo-polygalacturonase, the optimum temperature and pH were obtained at 50°C and pH 4.0, respectively. SDS-PAGE analysis showed that molecular weight of exo-polygalacturonase were 35 and 71 kDa. This study has revealed a significant production of exo-polygalacturonase by A. niger under SSF using cheap and easily available substrate and thus could found immense potential application in industrial sectors and biotechnology

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Changes in Phenolics and Fatty Acids Composition and Related Gene Expression during the Development from Seed to Leaves of Three Cultivated Cardoon Genotypes

Antioxidants ◽

10.3390/antiox9111096 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1096

Author(s):

Giulia Graziani ◽

Teresa Docimo ◽

Monica De Palma ◽

Francesca Sparvoli ◽

Luana Izzo ◽

...

Keyword(s):

Fatty Acids ◽

Wide Spectrum ◽

Sustainable Use ◽

Fatty Acid Desaturase ◽

Value Added ◽

Industrial Applications ◽

Transcriptional Analysis ◽

Total Phenols ◽

Superior Genotypes

Cultivated cardoon (Cynara cardunculus var. altilis) has long been used as a food and medicine remedy and nowadays is considered a functional food. Its leaf bioactive compounds are mostly represented by chlorogenic acids and coumaroyl derivatives, known for their nutritional value and bioactivity. Having antioxidant and hepatoprotective properties, these molecules are used for medicinal purposes. Apart from the phenolic compounds in green tissues, cultivated cardoon is also used for the seed oil, having a composition suitable for the human diet, but also valuable as feedstock for the production of biofuel and biodegradable bioplastics. Given the wide spectrum of valuable cardoon molecules and their numerous industrial applications, a detailed characterization of different organs and tissues for their metabolic profiles as well as an extensive transcriptional analysis of associated key biosynthetic genes were performed to provide a deeper insight into metabolites biosynthesis and accumulation sites. This study aimed to provide a comprehensive analysis of the phenylpropanoids profile through UHPLC-Q-Orbitrap HRMS analysis, of fatty acids content through GC-MS analysis, along with quantitative transcriptional analyses by qRT-PCR of hydroxycinnamoyl-quinate transferase (HQT), stearic acid desaturase (SAD), and fatty acid desaturase (FAD) genes in seeds, hypocotyls, cotyledons and leaves of the cardoon genotypes “Spagnolo”, “Bianco Avorio”, and “Gigante”. Both oil yield and total phenols accumulation in all the tissues and organs indicated higher production in “Bianco Avorio” and “Spagnolo” than in “Gigante”. Antioxidant activity evaluation by DPPH, ABTS, and FRAP assays mirrored total phenols content. Overall, this study provides a detailed analysis of tissue composition of cardoon, enabling to elucidate value-added product accumulation and distribution during plant development and hence contributing to better address and optimize the sustainable use of this natural resource. Besides, our metabolic and transcriptional screening could be useful to guide the selection of superior genotypes.

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Immobilization of Bacillus licheniformis using Fe3O4@SiO2 nanoparticles for the development of bacterial bioreactor

Oriental Journal Of Chemistry ◽

10.13005/ojc/350249 ◽

2019 ◽

Vol 35 (2) ◽

pp. 854-862

Author(s):

Ayoub Nadi ◽

Marouane Melloul ◽

Aicha Boukhriss ◽

Elmostafa El-Fahime ◽

Damien Boyer ◽

...

Keyword(s):

Bacillus Licheniformis ◽

Fe3o4 Nanoparticles ◽

Sol Gel ◽

Sio2 Nanoparticles ◽

Industrial Applications ◽

Basic Medium ◽

Magnetite Fe3o4 ◽

Sol Gel Process ◽

Co Precipitation

In the biotechnology field, nanoparticles with a strong magnetic moment can bring attractive and novel potentialities. They are detectable, manipulable, stimulable by a magnetic field and they could be applied as nano-tracers for medical imaging and nano-vectors for transporting therapeutic agents to a target. For our part, we applied Fe3O4 nanoparticles to immobilize bacteria of Moroccan strains in order to develop bacterial bioreactor. For this aim, we got through the synthesis and characterization of magnetite Fe3O4 nanoparticles by co-precipitation in basic medium. The obtained nanoparticles were encapsulated in silica by sol-gel process. The results of this step allowed us to use Fe3O4@SiO2 nanoparticles to immobilize Bacillus licheniformis by adsorption and separate it magnetically. The principle of this system gives us the opportunity to develop a bacterial bioreactor for industrial applications.

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