P4-209: Methazolamide protects neuronal and glial cells from amyloid toxicity in vitro and in vivo via mitochondria-mediated mechanisms

2015 ◽  
Vol 11 (7S_Part_18) ◽  
pp. P860-P861
Author(s):  
Silvia Fossati ◽  
Patrizia Giannoni ◽  
Maria E. Solesio ◽  
Sarah L. Cocklin ◽  
Erwin Cabrera ◽  
...  
Keyword(s):  
Glial Cells ◽  
Amyloid Toxicity ◽  
Toxicity In Vitro

scholarly journals Attenuation of β-Amyloid Toxicity In Vitro and In Vivo by Accelerated Aggregation

2017 ◽  
Vol 33 (4) ◽  
pp. 405-412 ◽  
Author(s):  
Aihua Yang ◽  
Chenxuan Wang ◽  
Baomin Song ◽  
Wendi Zhang ◽  
Yuanyuan Guo ◽  
...  
Keyword(s):  
Amyloid Toxicity ◽  
Β Amyloid ◽  
Toxicity In Vitro

Derivatives of Deoxypodophyllotoxin Induce Apoptosis Through Bcl-2/Bax Proteins Expression

2020 ◽  
Vol 20 ◽  
Author(s):  
Ya-Nan Li ◽  
Ni Ning ◽  
Lei Song ◽  
Yun Geng ◽  
Jun-Ting Fan ◽  
...  
Keyword(s):  
Annexin V ◽  
Mtt Method ◽  
Anthriscus Sylvestris ◽  
Anti Tumor Activity ◽  
Derivatives Of ◽  
Toxicity In Vitro ◽  
Ht 29 ◽  
Mcf 7

Background: Deoxypodophyllotoxin, isolated from theTraditional Chinese Medicine Anthriscus sylvestris, is well-known because of its significant antitumor activity with strong toxicity in vitro and in vivo. Objective: In this article, we synthesized a series of deoxypodophyllotoxin derivatives, and evaluated their antitumor effectiveness.Methods:The anti tumor activity of deoxypodophyllotoxin derivatives was investigated by the MTT method. Apoptosis percentage was measured by flow cytometer analysis using Annexin-V-FITC. Results: The derivatives revealed obvious cytotoxicity in the MTT assay by decreasing the number of late cancer cells. The decrease of Bcl-2/Bax could be observed in MCF-7, HepG2, HT-29 andMG-63 using Annexin V-FITC. The ratio of Bcl-2/Bax in the administration group was decreased, which was determined by the ELISA kit. Conclusion: The derivatives of deoxypodophyllotoxin could induce apoptosis in tumor cell lines by influencing Bcl-2/Bax.

The Toxic Effects of Two Dental Materials on Human Buccal Epithelium In Vitro and Monkey Buccal Epithelium In Vivo

1987 ◽  
Vol 14 (3) ◽  
pp. 166-167
Author(s):  
D. Arenholt-Bindslev ◽  
P. Hørsted-Bindslev ◽  
H.P. Philipsen
Keyword(s):  
Dental Materials ◽  
Microscopical Examination ◽  
Buccal Epithelium ◽  
Cytotoxic Effects ◽  
Buccal Epithelial Cells ◽  
Toxicity In Vitro ◽  
Toxic Reactions ◽  
Light Microscopical Examination

The aim of the present study was to compare the toxicity in vitro with the toxicity in vivo of two commercial chemicals marketed for use in the oral cavity (GLUMA BondR and 3M Etching LiquidR). Confluent cultures of human buccal epithelial cells were exposed to graded concentrations of GLUMA Bond or 3M Etching Liquid for 5 minutes. The cytotoxic effects induced by this treatment were observed (cytomorphology, proliferation rate). In vivo, monkey buccal epithelium was exposed to GLUMA Bond or 3M Etching Liquid for 5 minutes. Biopsies were taken after 24 hours, and the buccal epithelium processed for light microscopical examination. In both models, the toxic reactions to GLUMA Bond were far more extensive than those caused by 3M Etching Liquid.

Neuroprotective Effects of the FGF21 Analogue LY2405319

2021 ◽  
pp. 1-13
Author(s):  
Claire Rühlmann ◽  
David Dannehl ◽  
Marcus Brodtrück ◽  
Andrew C. Adams ◽  
Jan Stenzel ◽  
...  
Keyword(s):  
Glial Cells ◽  
Neuroprotective Effect ◽  
Amyloid Β ◽  
Fibroblast Growth Factor 21 ◽  
Neuroprotective Effects ◽  
Organotypic Brain Slice ◽  
Brain Slice Cultures ◽  
Abca1 Mrna

Background: To date, there are no effective treatments for Alzheimer’s disease (AD). Thus, a significant need for research of therapies remains. Objective: One promising pharmacological target is the hormone fibroblast growth factor 21 (FGF21), which is thought to be neuroprotective. A clinical candidate for medical use could be the FGF21 analogue LY2405319 (LY), which has a specificity and potency comparable to FGF21. Methods: The present study investigated the potential neuroprotective effect of LY via PPARγ/apoE/abca1 pathway which is known to degrade amyloid-β (Aβ) plaques by using primary glial cells and hippocampal organotypic brain slice cultures (OBSCs) from 30- and 50-week-old transgenic APPswe/PS1dE9 (tg) mice. By LY treatment of 52-week-old tg mice with advanced Aβ deposition, we further aimed to elaborate the effect of LY on AD pathology in vivo. Results: LY application to primary glial cells caused an upregulation of pparγ, apoE, and abca1 mRNA expression and significantly decreased number and area of Aβ plaques in OBSCs. LY treatment in tg mice increased cerebral [18F] FDG uptake and N-acetylaspartate/creatine ratio indicating enhanced neuronal activity and integrity. Although LY did not reduce the number of Aβ plaques in tg mice, the number of iba1-positive cells was significantly decreased indicating reduced microgliosis. Conclusion: These data identified LY in vitro as an activator of Aβ degrading genes leading to cerebral Aβ load amelioration in early and late AD pathology. Although Aβ plaque reduction by LY failed in vivo, LY may be used as therapeutic agent to treat AD-related neuroinflammation and impaired neuronal integrity.

Antimicrobial and antibiofilm activities of meropenem loaded-mesoporous silica nanoparticles against carbapenem-resistant Pseudomonas aeruginosa

2021 ◽  
pp. 088532822110038
Author(s):  
Mohammad Yousef Memar ◽  
Mina Yekani ◽  
Hadi Ghanbari ◽  
Edris Nabizadeh ◽  
Sepideh Zununi Vahed ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Carbapenem Resistant ◽  
Toxicity In Vitro ◽  
Different Levels

The aims of the present study were the determination of antimicrobial and antibiofilm effects of meropenem-loaded mesoporous silica nanoparticles (MSNs) on carbapenem resistant Pseudomonas aeruginosa ( P. aeruginosa) and cytotoxicity properties in vitro. The meropenem-loaded MSNs had shown antibacterial and biofilm inhibitory activities on all isolates at different levels lower than MICs and BICs of meropenem. The viability of HC-04 cells treated with serial concentrations as MICs and BICs of meropenem-loaded MSNs was 92–100%. According to the obtained results, meropenem-loaded MSNs display the significant antibacterial and antibiofilm effects against carbapenem resistant and biofilm forming P. aeruginosa and low cell toxicity in vitro. Then, the prepared system can be an appropriate option for the delivery of carbapenem for further evaluation in vivo assays.

scholarly journals Fabrication of novel vesicles of triptolide for antirheumatoid activity with reduced toxicity in vitro and in vivo

2016 ◽  
pp. 2663 ◽  
Author(s):  
Qianqian Liang ◽  
Li Zhang ◽  
Tengteng Wang ◽  
Qiang Li ◽  
Jing Huang ◽  
...  
Keyword(s):  
Reduced Toxicity ◽  
Toxicity In Vitro

Dendrimeric poly(propylene-imines) as effective delivery agents for DNAzymes: Toxicity, in vitro transfection and in vivo delivery

2006 ◽  
Vol 116 (2) ◽  
pp. e26-e28 ◽  
Author(s):  
F. Tack ◽  
A. Bakker ◽  
S. Maes ◽  
N. Dekeyser ◽  
M. Bruining ◽  
...  
Keyword(s):  
Effective Delivery ◽  
Poly Propylene ◽  
Toxicity In Vitro ◽  
In Vitro Transfection ◽  
In Vivo Delivery

The Assessment of Bioreductive Drug Toxicity in vitro and in Experimental Tumours in vivo

1990 ◽  
pp. 203-212 ◽  
Author(s):  
I. J. Stratford ◽  
G. E. Adams ◽  
J. C. M. Bremner ◽  
S. Cole ◽  
Li Ding ◽  
...  
Keyword(s):  
Drug Toxicity ◽  
Toxicity In Vitro

scholarly journals Spike Protein Targeting "Nano-Glue" that Captures and Promotes SARS-CoV-2 Elimination

2021 ◽  
Author(s):  
Guofang Zhang ◽  
Yalin Cong ◽  
Guoli Cao ◽  
Liang Li ◽  
Peng Yu ◽  
...  
Keyword(s):  
Protein Targeting ◽  
Binding Capacity ◽  
Spike Protein ◽  
Therapeutic Drug ◽  
Receptor Binding Domain ◽  
Airway Epithelial ◽  
Β Sheet ◽  
Toxicity In Vitro

The global emergency caused by the SARS-CoV-2 pandemics can only be solved with adequate preventive and therapeutic strategies, both currently missing. The electropositive Receptor Binding Domain (RBD) of SARS-CoV-2 spike protein with abundant β-sheet structure serves as target for COVID-19 therapeutic drug design. Here, we discovered that ultrathin 2D CuInP2S6 (CIPS) nanosheets as a new agent against SARS-CoV-2 infection, which also able to promote viral host elimination. CIPS exhibits extremely high and selective binding capacity with the RBD of SARS-CoV-2 spike protein, with consequent inhibition of virus entry and infection in ACE2-bearing cells and human airway epithelial organoids. CIPS displays nano-viscous properties in selectively binding with spike protein (KD < 1 pM) with negligible toxicity in vitro and in vivo. Further, the CIPS-bound SARS-CoV-2 was quickly phagocytosed and eliminated by macrophages, suggesting CIPS could be successfully used to capture and facilitate the virus host elimination with possibility of triggering anti-viral immunization. Thus, we propose CIPS as a promising nanodrug for future safe and effective anti-SARS-CoV-2 therapy, as well as for use as disinfection agent and surface coating material to constrain the SARS-CoV-2 spreading.

scholarly journals Protective Effects of Thiol Compounds on Chromate-Induced Toxicity In Vitro and In Vivo

1994 ◽  
Vol 102 ◽  
pp. 247 ◽  
Author(s):  
Nobuyuki Susa ◽  
Shunji Ueno ◽  
Yoshinori Furukawa
Keyword(s):  
Protective Effects ◽  
Thiol Compounds ◽  
Toxicity In Vitro
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