scholarly journals Effects of Schizochytrium microalgae and sunflower oil as sources of unsaturated fatty acids for the sustainable mitigation of ruminal biogases methane and carbon dioxide

2017 ◽  
Vol 168 ◽  
pp. 1389-1397 ◽  
Author(s):  
M.M.Y. Elghandour ◽  
L.H. Vallejo ◽  
A.Z.M. Salem ◽  
M.Z.M. Salem ◽  
L.M. Camacho ◽  
...  
2018 ◽  
pp. 91-97
Author(s):  
Artyom Dmitrievich Ivakhnov ◽  
Kristina Sergeevna Sadkova ◽  
Alina Sergeyevna Sobashnikova ◽  
Tat'yana Eduardovna Skrebets ◽  
Mikhail Vladislavovich Bogdanov

Comparative researches of ways of oil extraction from the fulfilled fruits of cloudberries (Rubus chamaemorus) with application of hexane and supercritical carbon dioxide as solvents are executed. Optimization is performed and optimum conditions of supercritical fluid extraction of oil are defined with use of central composite design of 2nd order. Pressure of carbon dioxide of 350 atm, temperature 85 °C, duration of extraction of 80 min are the optimum conditions of carrying out of the process. The yield of oil is 9.0%. Quality key indicators of the received oil were defined. The difference between the oil received by the SKF-CO2 method and the oil received by hexane extraction consists in improvement of organoleptic properties, the raised share of the combined fatty acids at decrease of a share of the free acids and high content of unsaturated fatty acids. It is shown that supercritical carbon dioxide can be an alternative to the hydrocarbons which are traditionally used for these purposes.


Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1836
Author(s):  
Lijuan Han ◽  
Qingqing Han ◽  
Yongjing Yang ◽  
Honglun Wang ◽  
ShuLin Wang ◽  
...  

Characterization of the structure and pharmacological activity of Berberis dasystachya Maxim., a traditional Tibetan medicinal and edible fruit, has not yet been reported. In this study, central composite design (CCD) combined with response surface methodology (RSM) was applied to optimize the extraction conditions of B. dasystachya oil (BDSO) using the supercritical carbon dioxide (SC-CO2) extraction method, and the results were compared with those obtained by the petroleum ether extraction (PEE) method. The chemical characteristics of BDSO were analyzed, and its antioxidant activity and in vitro cellular viability were studied by DPPH, ABTS, reducing power assay, and MTT assay. The results showed that the maximum yield of 12.54 ± 0.56 g/100 g was obtained at the optimal extraction conditions, which were: pressure, 25.00 MPa; temperature 59.03 °C; and CO2 flow rate, 2.25 SL/min. The Gas chromatography (GC) analysis results showed that BDSO extracted by the SC-CO2 method had higher contents of unsaturated fatty acids (85.62%) and polyunsaturated fatty acids (57.90%) than that extracted by the PEE method. The gas chromatography used in conjunction with ion mobility spectrometry (GC–IMS) results showed that the main volatile compounds in BDSO were aldehydes and esters. BDSO also exhibited antioxidant ability in a dose-dependent manner. Moreover, normal and cancer cells incubated with BDSO had survival rates of more than 85%, which indicates that BDSO is not cytotoxic. Based on these results, the BDSO extracted by the SC-CO2 method could potentially be used in other applications, e.g., those that involve using berries of B. dasystachya.


1997 ◽  
Vol 77 (2) ◽  
pp. 287-292 ◽  
Author(s):  
Dirk Hoehler ◽  
Ronald R. Marquardt ◽  
Andrew A.F. Rohlich

The objective of this study was to determine whether lipid peroxidation is one mode of action in ochratoxin A (OA) toxicity in vivo. Lipid peroxidation was monitored by analyzing malondialdehyde (MDA) in different tissues by HPLC. A refinement study on the MDA assay was carried out, which showed the importance of the addition of an iron catalyst for the decomposition of hydroperoxides to yield a maximum amount of MDA from a given sample. The rat experiment was designed in a 2 × 2 factorial arrangement using 4 × 6 animals. The four different diets were fed for 21 d and contained either 1% corn oil and 9% tallow (Diets I and III) or 10% corn oil (Diets II and IV); in groups III and IV, 5 mg OA were added per kilogram of diet. For the chick experiment 4 × 8 Leghorn cockerels received diets for 14 d with no added sunflower oil (Diets I and III), whereas the diets of groups II and IV were supplemented with 2.5% sunflower oil. In groups III and IV, 2.5 mg OA were added per kilogram of diet. In both experiments OA decreased the performance of the animals significantly. In the rat experiment an increased lipid peroxidation due to a higher dietary level of unsaturated fatty acids could be obtained, when muscle samples were oxidatively stressed with Fe3+ and ascorbic acid. In the chick experiment there were very clear effects of the dietary treatment on the MDA concentrations of different tissues, as both a higher supply with unsaturated fatty acids and OA increased most of the MDA values significantly. These data suggest that lipid peroxides are formed in vivo by OA, but the effects may vary considerably from species to species, and may also be influenced by other factors. Key words: Ochratoxin A, lipid peroxidation, malondialdehyde, rat, chick


1989 ◽  
Vol 260 (3) ◽  
pp. 647-655 ◽  
Author(s):  
J M Street ◽  
D W Johnson ◽  
H Singh ◽  
A Poulos

The metabolism of 1-11C-labelled derivatives of palmitic (C16:0), arachidonic (C20:4,n-6) lignoceric (C21:0) and tetracosatetraenoic (C24:4,n-6) acids was studied in normal skin fibroblast cultures and in cultures of fibroblasts from peroxisome-deficient (Zellweger's syndrome) patients. Radiolabelled products of the fatty acids included carbon dioxide. C14-24 saturated and mono-unsaturated fatty acids formed from released acetate either by synthesis de novo or by elongation of endogenous fatty acids, fatty acids formed by 2-6-carbon elongation of added substrates, and a number of water-soluble compounds, some of which were tentatively identified as the amino acids glutamine, glutamic acid and asparagine. The labelled amino acids were found predominantly in the culture medium. Zellweger's syndrome fibroblasts showed a marked decrease in radiolabelled carbon dioxide and water-soluble-product formation from (I-14C)-labelled arachidonic, tetracosatetraenoic and lignoceric acids but not from [I-14C]palmitic acid, and the production of radiolabelled C14-18 fatty acids was also diminished. However, the elongation of individual fatty acids was either normal or above normal. Our data support the view that the oxidation of 20:4, 24:4 and 24:0 fatty acids in cultured skin fibroblasts takes place largely in peroxisomes, and further that the acetyl-CoA released by the beta-oxidation process is available for the synthesis of fatty acids and amino acids. We speculate that the generation of C2 units used for synthesis is a major peroxisomal function and that this function is absent or greatly impaired in Zellweger's syndrome cells.


2019 ◽  
Vol 2019 (4) ◽  
pp. 36-47
Author(s):  
Anatoly Gaganov ◽  
Zinaida Zverkova ◽  
Bella Osipyan

Studies were conducted on the use of oil obtained from rapeseed cultivar 'Podmoskovnyj' selection of Federal Williams Research Center of Forage Production and Agroecology in feeding broiler chickens. The experiments were carried out on chickens cross-country "ROSS-308". They were grown in a cell battery. For the experiment, 5 groups were formed. In the start and finish periods, the control group received 2.5 and 3.3% sunflower oil. In the experimental groups, sunflower oil was replaced with rapeseed in the following amounts: the first — 1.0 and 1.0%, the second — 1.5 and 1.7%, the third — 2.0 and 2.5% and the fourth — 2.5 and 3.3%. Feeding was carried out with dry loose feed from a day old. Studies have found that rapeseed oil had less saturated and polyunsaturated fatty acids, but more unsaturated fatty acids. The amount of erucic acid was 0.20%. The digestibility of nutrients was high and practically did not differ from the control variant, as was the use of nitrogen. The inclusion of rapeseed oil in the composition of feed for broilers did not have a significant impact on the increase in live weight and feed costs. The cost of feed with increasing rapeseed oil in their composition decreased, and the efficiency of converting feed into products increased slightly. Compound feeds with rapeseed oil were not inferior in terms of the efficiency of using compound feed containing sunflower oil.


2018 ◽  
Vol 66 (8) ◽  
pp. 1751-1759 ◽  
Author(s):  
Ali S. Gomaa ◽  
Ahmed E. Kholif ◽  
Abdelkader M. Kholif ◽  
Reda Salama ◽  
Hamza A. El-Alamy ◽  
...  

1976 ◽  
Vol 16 (82) ◽  
pp. 691 ◽  
Author(s):  
N Guirguis

The ME values of tallow, sunflower oil, soyabean, rapeseed and fish meals were determined by chick assay at various levels of dietary inclusion with both sexes. Female chicks metabolized significantly (P < 0.01) more energy from tallow and fish meal than did male chicks. Energy utilized from other feedstuffs tested did not differ between sexes. Less energy was available from tallow as its level of dietary inclusion increased; this could partly be due to altering the ratio of saturated to unsaturated fatty acids in the diet, leading to reduced absorption of palmitic and stearic acids present in tallow. There were significant interactions between sex of chicks and the levels at which tallow and fish meal were included in the diet. A possible interrelationship between the two feedstuffs in relation to the sex differences in absorption of dietary fat is discussed.


2017 ◽  
Vol 42 (3) ◽  
pp. 185 ◽  
Author(s):  
L. Khotijah ◽  
E. I. Pandiangan ◽  
D. A. Astuti ◽  
K. G. Wiryawan

The study was aimed to evaluate the increasing levels of ration energy, with fat-rich unsaturated fatty acids from sunflower oil on the nutrient intake, rumen fermentability and performance of ewes. The experiment used fifteen ewes of 1.5 years old, primiparous, caged individually with their lambs and conducted three treatments and five replications from a completely randomized designed. Those treatments included R1= ration with 0% sunflower oil addition, R2= ration with 4%sunflower oil addition, R3= ration with 6% sunflower oil addition. The variables consisted of dry matter intake, nutrients, ewes performances (birth weight, weaning weight, decreased of body weight), total and partial VFA, the ratio of acetate: propionate, methane production, protozoa and bacterial population. Data were analyzed statistically and descriptively. The results showed that the treatments did not influence dry matter intake and nutrients,. rumen fermentability and ewes performances. Although the treatments increased (P<0.01) fatconsumption and reduced shrinkage of body weight. It can be concluded that the adding sunflower oil as the resource of unsaturated fatty acids up to 6% of the ration was not negatively affected the fermentability of rumen, moreover can improve ewes body condition and, accelerated body weight recovery


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