Investigation of saliva, tongue swabs and buccal swabs as alternative specimen types to nasopharyngeal swabs for SARS-CoV-2 testing

Introduction The current gold standard for detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA involves subjecting nasopharyngeal or oropharyngeal swabs to reverse transcription quantitative PCR (RT-qPCR). However, both sample types need to be collected by trained professionals. Using self-collected buccal swabs as an alternative could simplify and accelerate diagnosis of coronavirus disease 2019 (COVID-19). Objective To assess self-collected buccal swab samples as an alternative method for SARS-CoV-2 detection in patients with COVID-19. Methods Buccal swab samples were self-collected by 73 patients with COVID-19. Total RNA was extracted using Qiagen kits. RNA encoding the SARS-CoV-2 Env protein and human RNase P as an internal control was amplified using the TRUPCR® SARS-CoV-2 RT-qPCR kit version 2.1 and a Bio-Rad CFX96 Real-Time Detection System. Result The sensitivity of RT-qPCR from buccal swabs was 58.9% (43/73; 95% confidence interval [CI] 46.77%–70.27%) and that of RT-qPCR from saliva was 62.90% (39/62; 95% CI 49.69%–74.84%) taking positive SARS-CoV-2 RT-qPCR from nasopharyngeal swabs as the gold standard. Conclusion Self-collected buccal swabs are promising alternatives to nasopharyngeal or oropharyngeal swabs for SARS CoV-2 detection.

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Metabarcoding-based dietary analysis of hen harrier (Circus cyaneus) in Great Britain using buccal swabs from chicks

Conservation Genetics ◽

10.1007/s10592-019-01215-y ◽

2019 ◽

Vol 20 (6) ◽

pp. 1389-1404

Author(s):

Kevin Nota ◽

Stephen Downing ◽

Arati Iyengar

Keyword(s):

Great Britain ◽

Dietary Analysis ◽

Buccal Swabs ◽

Circus Cyaneus ◽

Hen Harrier

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Related Donor Human Leukocyte Antigen Typing: Developing a Process for Utilization of Buccal Swabs Versus Blood Tubes

Biology of Blood and Marrow Transplantation ◽

10.1016/j.bbmt.2015.11.1046 ◽

2016 ◽

Vol 22 (3) ◽

pp. S457-S458

Author(s):

Kim Schmit-Pokorny ◽

Cathy Gebhart ◽

Sophia Hauxwell ◽

Jeanine Schademann ◽

JoAnn Tate

Keyword(s):

Human Leukocyte Antigen ◽

Human Leukocyte ◽

Human Leukocyte Antigen Typing ◽

Leukocyte Antigen ◽

Buccal Swabs ◽

Related Donor

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Identification of genetic markers associated with ibrutinib-related cardiovascular toxicity.

Journal of Clinical Oncology ◽

10.1200/jco.2021.39.15_suppl.7526 ◽

2021 ◽

Vol 39 (15_suppl) ◽

pp. 7526-7526

Author(s):

Issam Hamadeh ◽

Ryan Jacobs ◽

Bei Hu ◽

Tamara Kay Moyo ◽

Amy Soni ◽

...

Keyword(s):

Data Extraction ◽

Genetic Risk Score ◽

Univariate Analysis ◽

Fold Increase ◽

Lymphocytic Leukemia ◽

Nucleotide Polymorphisms ◽

Clinical Tool ◽

Specimen Collection ◽

Cardiovascular Side Effects ◽

Buccal Swabs

7526 Background: Cardiovascular side effects (CVSEs: atrial fibrillation, hypertension, etc.) are common in patients with chronic lymphocytic leukemia (CLL) treated with ibrutinib and often lead to dose reductions or discontinuation. However, the etiology of ibrutinib related CVSEs has not been elucidated. This study sought to interrogate the association between ibrutinib related CVSEs and polymorphisms in genes of the Bruton Tyrosine Kinase (BTK) signaling pathway (identified through Ingenuity Pathway Analysis) Methods: Newly diagnosed and relapsed patients with CLL who underwent treatment with ibrutinib between December 2019 and November 2020 at Levine Cancer Institute were identified. Buccal swabs were collected through an IRB approved specimen collection protocol. Data extraction included: demographics, CLL stage, cytogenetics, previous treatments, ibrutinib start dates and dose, drug related SEs, and other medications. DNA isolated from buccal swabs was genotyped for 40 single nucleotide polymorphisms (SNPs) in GATA4, SGK1, KCNQ1, KCNA4, NPPA and SCN5A genes using a custom NGS panel. Logistic regression analysis evaluated the association between SNPs and CVSEs. Results: In 50 evaluable patients, the median age was 71 years (range:48-90) and 50% received frontline ibrutinib monotherapy. CVSEs occurred in 20% of patients (n=10). In univariate analysis, 4 SNPs in 3 genes were significantly associated with CVSEs (Table). Because the genes were in the same pathway, a genetic risk score was developed which indicated that patients with at least 2 SNPs had a 12-fold increase in risk of CVSEs (Table). Conclusions: Our findings provide insights into the genetic determinants of ibrutinib related CVSEs. If replicated in a larger study, this will facilitate utility of pharmacogenetic testing (for GATA4, KCNQ1 and KCNA5 polymorphisms ) as a clinical tool to individualize ibrutinib treatment.[Table: see text]

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The Relationship Between Genotype and Positive Psychological Development in National-Level Swimmers

European Psychologist ◽

10.1027/1016-9040.11.2.143 ◽

2006 ◽

Vol 11 (2) ◽

pp. 143-148 ◽

Cited By ~ 26

Author(s):

Jim Golby ◽

Michael Sheard

Keyword(s):

National Level ◽

Psychological Theory ◽

Self Esteem ◽

Psychological Development ◽

Mental Toughness ◽

Positive Affectivity ◽

Buccal Swabs ◽

Preliminary Study ◽

The Relationship ◽

Psychological Questionnaires

We report a preliminary study of the relationship between genotype (using a functional polymorphism of the serotonin transporter 5-HTT gene) and positive psychological development in high-achieving adolescent swimmers. Thirty-one volunteers of both genders, aged 10-24 years, completed a battery of positive psychological questionnaires measuring hardiness, mental toughness, self-esteem, self-efficacy, dispositional optimism, and positive affectivity. DNA samples were obtained via buccal swabs. The sample was initially split into three groups on the basis of 5-HTT genotype: SS, SL, and LL. Multivariate statistics revealed a discernible trend of a relationship between LL genotype and higher levels of positive psychological development. The nonsignificant overall effect is interpreted in terms of sample size and age of participants. The implications of these results are discussed relative to previous findings and in terms of psychological theory.

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DNA by Mail: Ensure DNA Integrity by use of Self-Drying Buccal Swabs

Lettters in Health & Biological Sciences ◽

10.15436/2475-6245.17.020 ◽

2017 ◽

Vol 2 (2) ◽

pp. 71-77 ◽

Cited By ~ 1

Author(s):

Nicole Bauer ◽

◽

Rene Rohrmanstorfer ◽

Stefanie Zelch ◽

Daniel Wallerstorfer ◽

...

Keyword(s):

Dna Integrity ◽

Use Of Self ◽

Buccal Swabs

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Micronucleus Analysis in Buccal Swabs of Paddy Farmers from the East Coast of Malaysia

Jurnal Sains Kesihatan Malaysia ◽

10.17576/jskm-2018-16si-02 ◽

2018 ◽

Vol 16 (Special Issue) ◽

pp. 9-14

Author(s):

Asmah Hamid ◽

Kholilul Syafiq Baharuddin ◽

Nor Fadilah Rajab ◽

Syarif Husin Lubis ◽

Ismarulyusda Ishak ◽

...

Keyword(s):

East Coast ◽

Buccal Swabs ◽

Paddy Farmers

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Attempts to infect pigs with Coxsackie virus type B5

Journal of Hygiene ◽

10.1017/s0022172400023871 ◽

1974 ◽

Vol 73 (1) ◽

pp. 85-96 ◽

Cited By ~ 11

Author(s):

A. J. M. Garland ◽

J. A. Mann

Keyword(s):

Virus Type ◽

Neutralizing Antibody ◽

Coxsackie Virus ◽

Virus Population ◽

Serological Relationship ◽

Anamnestic Response ◽

Transient Rise ◽

Buccal Swabs ◽

Antibody Titres ◽

Vesicular Disease

SUMMARYDespite the existence of a close serological relationship between the entero-viruses Swine Vesicular Disease (SVD) and Coxsackie type B5 (Cx B5), the administration of this Coxsackie virus type to susceptible pigs by various routes failed to produce clinical disease.Viraemia was not detected after exposure but virus was recovered intermittently from faeces and buccal swabs. A mixed virus population was demonstrated in faecal cultures from some pigs, including Coxsackie virus type B5 and other agents, presumably native pig enteroviruses. The Coxsackie virus persisted in faeces in declining amounts for up to 8 days after primary exposure.Serum neutralizing antibody showed a transient rise to Coxsackie virus, reaching a peak at 14 days and declining below demonstrable titres by 28 days after exposure. The antibody titres attained were proportional to the dose of virus administered and the degree of neutralization was very similar to both SVD and Cx B5 viruses.On cross challenge by exposure to SVD virus 28 days after exposure to Cx B5 virus, most animals (5/6) succumbed with typical vesicular lesions, although the serum neutralizing antibody titres showed a characteristically anamnestic response to both viruses.

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