Reliable detection of Listeria monocytogenes by a portable paper-based multi-biocatalyst platform integrating three biomarkers: gene hly, acetoin, and listeriolysin O protein

The present study aimed to investigate the effect of probiotic lactic acid bacteria (LAB) addition on Listeria monocytogenes translocation and its toxin listeriolysin O (LLO), proinflammatory factors, immune organ indexes and serum immunoglobulins in farmed rabbits. Five treatments included negative control (NC), positive control (PC) with L. monocytogenes infection and supplemental LAB at 3.0 × 106 (low-LAB, L-LAB), 3.0 × 108 (medium-LAB, M-LAB) and 3.0 × 1010 (high-LAB, H-LAB) CFU/kg of diet, respectively. The LAB was a mixture of equal amounts of Lactobacillus acidophilus (ACCC11073), Lactobacillus plantarum (CICC21863) and Enterococcus faecium (CICC20430). A total of 180 weaned rabbits (negative for L. monocytogenes) were randomly assigned to 5 groups with 6 replicates of 6 rabbits each in response to the 5 treatments. L. monocytogenes infection occurred on the first day of feeding trial and dietary LAB supplementation lasted for 14 days. The results showed that on days 7 and 14 post administration, L. monocytogenes in caecum, liver, spleen and lymph nodes was reduced in M-LAB and H-LAB compared to PC (P < 0.05), and linear and quadratic reducing trends were found in liver on day 7 (P ≤ 0.002). On day 14, mucosa LLO mRNA expression and serum TNFα, IL1β and IFNγ were reduced in the three LAB treatments (P < 0.05), and linear and quadratic trends were found on TNFα and IL1β (P ≤ 0.025); indexes of thymus and spleen, serum IgA and IgG were increased in the LAB treatments (P < 0.05). It is concluded that LAB can be used to alleviate L. monocytogenes infection and to improve the immune function of farmed animals.

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The Listeria monocytogenes hemolysin has an acidic pH optimum to compartmentalize activity and prevent damage to infected host cells

The Journal of Cell Biology ◽

10.1083/jcb.200201081 ◽

2002 ◽

Vol 156 (6) ◽

pp. 1029-1038 ◽

Cited By ~ 190

Author(s):

Ian J. Glomski ◽

Margaret M. Gedde ◽

Albert W. Tsang ◽

Joel A. Swanson ◽

Daniel A. Portnoy

Keyword(s):

Listeria Monocytogenes ◽

Bacterial Pathogen ◽

Cytolytic Activity ◽

Host Cells ◽

Adaptive Mutation ◽

Acidic Ph ◽

Listeriolysin O ◽

Lysosomal Hydrolases ◽

Ph Optimum ◽

Neutral Ph

Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from a phagosome and grows in the host cell cytosol. The pore-forming cholesterol-dependent cytolysin, listeriolysin O (LLO), mediates bacterial escape from vesicles and is ∼10-fold more active at an acidic than neutral pH. By swapping dissimilar residues from a pH-insensitive orthologue, perfringolysin O (PFO), we identified leucine 461 as unique to pathogenic Listeria and responsible for the acidic pH optimum of LLO. Conversion of leucine 461 to the threonine present in PFO increased the hemolytic activity of LLO almost 10-fold at a neutral pH. L. monocytogenes synthesizing LLO L461T, expressed from its endogenous site on the bacterial chromosome, resulted in a 100-fold virulence defect in the mouse listeriosis model. These bacteria escaped from acidic phagosomes and initially grew normally in cells and spread cell to cell, but prematurely permeabilized the host membrane and killed the cell. These data show that the acidic pH optimum of LLO results from an adaptive mutation that acts to limit cytolytic activity to acidic vesicles and prevent damage in the host cytosol, a strategy also used by host cells to compartmentalize lysosomal hydrolases.

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Listeriolysin O is essential for virulence of Listeria monocytogenes: direct evidence obtained by gene complementation.

Infection and Immunity ◽

10.1128/iai.57.11.3629-3636.1989 ◽

1989 ◽

Vol 57 (11) ◽

pp. 3629-3636 ◽

Cited By ~ 281

Author(s):

P Cossart ◽

M F Vicente ◽

J Mengaud ◽

F Baquero ◽

J C Perez-Diaz ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Direct Evidence ◽

Listeriolysin O ◽

Gene Complementation

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Effect of honokiol on exotoxin proteins listeriolysin O and p60 secreted by Listeria monocytogenes

Journal of Medical Microbiology ◽

10.1099/jmm.0.000183 ◽

2015 ◽

Vol 64 (12) ◽

pp. 1474-1480

Author(s):

Rizeng Meng ◽

Ziwen Zhao ◽

Na Guo ◽

Zonghui Liu ◽

Xingchen Zhao ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Listeriolysin O

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Listeriolysin O derived from Listeria monocytogenes inhibits the effector phase of an experimental allergic rhinitis induced by ovalbumin in mice

Clinical & Experimental Immunology ◽

10.1111/j.1365-2249.2006.03092.x ◽

2006 ◽

Vol 144 (3) ◽

pp. 475-484 ◽

Cited By ~ 13

Author(s):

K. Yamamoto ◽

I. Kawamura ◽

T. Tominaga ◽

T. Nomura ◽

J. Ito ◽

...

Keyword(s):

Allergic Rhinitis ◽

Listeria Monocytogenes ◽

Listeriolysin O ◽

Effector Phase ◽

Experimental Allergic

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A Randomized Phase 2 Study of ADXS11-001 Listeria monocytogenes–Listeriolysin O Immunotherapy With or Without Cisplatin in Treatment of Advanced Cervical Cancer

International Journal of Gynecological Cancer ◽

10.1097/igc.0000000000001235 ◽

2018 ◽

Vol 28 (4) ◽

pp. 764-772 ◽

Cited By ~ 34

Author(s):

Partha Basu ◽

Ajay Mehta ◽

Minish Jain ◽

Sudeep Gupta ◽

Rajnish V. Nagarkar ◽

...

Keyword(s):

Cervical Cancer ◽

Listeria Monocytogenes ◽

Adverse Events ◽

Progression Free Survival ◽

Combination Group ◽

Listeriolysin O ◽

Phase 2 ◽

Safety And Efficacy ◽

Treatment Groups ◽

Phase 2 Study

ObjectivesA global unmet medical need exists for effective treatments for persistent, recurrent, or metastatic cervical cancer, as patients have a short life expectancy. Recently, immunotherapies have shown promising survival benefits for patients with advanced forms of cancer. Axalimogene filolisbac (ADXS11-001), aListeria monocytogenesimmunotherapy with a broad effect on the immune system, is under investigation for treatment of human papillomavirus–associated cancers including cervical cancer.MethodsThis phase 2 study evaluated the safety and efficacy of ADXS11-001, administered with or without cisplatin, in patients with recurrent/refractory cervical cancer following prior chemotherapy and/or radiotherapy. A total of 109 patients were treated, and 69 were evaluable for tumor response at equal to or more than 3 months postbaseline.ResultsMedian overall survival (OS) was comparable between treatment groups (ADXS11-001: 8.28 months; 95% confidence interval [CI], 5.85–10.5 months; ADXS11-001 + cisplatin: 8.78 months; 95% CI, 7.4–13.3 months). The 12- and 18-month milestone OS rates were 30.9% versus 38.9%, and 23.6% versus 25.9% for each group, respectively (34.9% and 24.8% combined). Median progression-free survival (6.10 vs 6.08 months) and the overall response rate (17.1% vs 14.7%) were similar for both groups. ADXS11-001 was generally well tolerated; adverse events were predominantly mild to moderate in severity and not related to treatment. More adverse events were reported in the combination group (429 vs 275).ConclusionsThese promising safety and efficacy results, including the encouraging 12-month 34.9% combined OS rate, warrant further investigation of ADXS11-001 for treatment of recurrent/refractory cervical cancer.

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Listeriolysin O-Based Latex Agglutination Test for the Rapid Detection of Listeria monocytogenes in Foods

Journal of Food Protection ◽

10.4315/0362-028x-60.9.1038 ◽

1997 ◽

Vol 60 (9) ◽

pp. 1038-1040 ◽

Cited By ~ 9

Author(s):

GHASSAN M. MATAR ◽

PEGGY S. HAYES ◽

WILLIAM F. BIBB ◽

BALA SWAMINATHAN

Keyword(s):

Monoclonal Antibody ◽

Listeria Monocytogenes ◽

Rapid Detection ◽

Rapid Test ◽

Food Samples ◽

Cross Reactivity ◽

Latex Agglutination ◽

Listeriolysin O ◽

Agglutination Assay ◽

Latex Beads

A latex agglutination-based test for the rapid detection of Listeria monocytogenes in foods was developed. An antilisteriolysin O (LLO) monoclonal antibody (HID5E12D7; IgG2b) covalently bound to polystyrene amidine-modified latex beads was used in a slide agglutination assay. The latex reagent detected 0.1 ng/ml of LLO in phosphate-buffered saline plus bovine serum albumin. It reacted with culture supernatants of L. monocytogenes but not with other Listeria species or Streptococcus groups A through G. The listeriolysin O latex agglutination assay (LLOLAT) was applied to 24-h and 48-h USDA primary enrichment cultures of 208 food samples obtained from refrigerators of listeriosis patients enrolled in a study to determine the role of foods in sporadic listeriosis. Of 19 samples positive by cultural techniques, 17 were positive by the LLOLAT. Cultures with low (<0.3 CFU/g) levels of L. monocytogenes were positive in the LLOLAT. No cross-reactivity occurred when using a heterogeneous monoclonal antibody. The LLOLAT is a sensitive, specific and rapid test and may be useful for screening foods for L. monocytogenes.

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