Insulin releasing and alpha-glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo

2010 ◽  
Vol 128 (1) ◽  
pp. 154-159 ◽  
Author(s):  
Mei-mei Si ◽  
Jian-shu Lou ◽  
Chang-Xin Zhou ◽  
Juan-Na Shen ◽  
Hong-Hai Wu ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Ethyl Acetate Fraction ◽  
Acorus Calamus ◽  
Alpha Glucosidase

Insulin sensitizing activity of ethyl acetate fraction of Acorus calamus L. in vitro and in vivo

2009 ◽  
Vol 123 (2) ◽  
pp. 288-292 ◽  
Author(s):  
Hao-Shu Wu ◽  
Di-Feng Zhu ◽  
Chang-Xin Zhou ◽  
Chu-Rui Feng ◽  
Yi-Jia Lou ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Acorus Calamus ◽  
Insulin Sensitizing Activity

scholarly journals Perilla frutescens Leaf Extract and Fractions: Polyphenol Composition, Antioxidant, Enzymes (α-Glucosidase, Acetylcholinesterase, and Tyrosinase) Inhibitory, Anticancer, and Antidiabetic Activities

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 315
Author(s):  
Zhenxing Wang ◽  
Zongcai Tu ◽  
Xing Xie ◽  
Hao Cui ◽  
Kin Weng Kong ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Animal Experiments ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Glycogen Accumulation ◽  
Inhibitory Ability

This study aims to evaluate the bioactive components, in vitro bioactivities, and in vivo hypoglycemic effect of P. frutescens leaf, which is a traditional medicine-food homology plant. P. frutescens methanol crude extract and its fractions (petroleum ether, chloroform, ethyl acetate, n-butanol fractions, and aqueous phase residue) were prepared by ultrasound-enzyme assisted extraction and liquid–liquid extraction. Among the samples, the ethyl acetate fraction possessed the high total phenolic (440.48 μg GAE/mg DE) and flavonoid content (455.22 μg RE/mg DE), the best antioxidant activity (the DPPH radical, ABTS radical, and superoxide anion scavenging activity, and ferric reducing antioxidant power were 1.71, 1.14, 2.40, 1.29, and 2.4 times higher than that of control Vc, respectively), the most powerful α-glucosidase inhibitory ability with the IC50 value of 190.03 μg/mL which was 2.2-folds higher than control acarbose, the strongest proliferative inhibitory ability against MCF-7 and HepG2 cell with the IC50 values of 37.92 and 13.43 μg/mL, which were considerable with control cisplatin, as well as certain inhibition abilities on acetylcholinesterase and tyrosinase. HPLC analysis showed that the luteolin, rosmarinic acid, rutin, and catechin were the dominant components of the ethyl acetate fraction. Animal experiments further demonstrated that the ethyl acetate fraction could significantly decrease the serum glucose level, food, and water intake of streptozotocin-induced diabetic SD rats, increase the body weight, modulate their serum levels of TC, TG, HDL-C, and LDL-C, improve the histopathology and glycogen accumulation in liver and intestinal tissue. Taken together, P. frutescens leaf exhibits excellent hypoglycemic activity in vitro and in vivo, and could be exploited as a source of natural antidiabetic agent.

scholarly journals Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Exerts Anti-Breast Cancer Effect via miR-200c-PDE7B/PD-L1-AKT/MAPK Axis

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Yue Yang ◽  
Ting Fang ◽  
Yi-Lan Cao ◽  
Ya-Xin Lv ◽  
Qing-Qi Chang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Colony Formation ◽  
Ethyl Acetate Fraction ◽  
Aqueous Extracts ◽  
Optimal Ratio ◽  
Hedyotis Diffusa ◽  
4T1 Cells

Background. Hedyotis diffusa (HD) Willd. and Scutellaria barbata (SB) D. Don in different ratios have been frequently used to treat various cancers in clinical Traditional Chinese Medicine prescriptions. However, the optimal ratio, active fraction, and molecular mechanisms associated with the anti-breast cancer role of this herbal couplet have not been elaborated. Methods. To screen out the optimal ratio of this herbal couplet, we compare aqueous extracts of HD, SB, or HD plus SB in different weight ratios (HS11, HS12, HS21) for their anticancer effects on murine breast cancer 4T1 cells in vitro and in vivo. EA11, the ethyl acetate fraction from HS11 (the aqueous extract of the couplet at an equal weight ratio), is further assessed for its antiproliferative effect as well as the antitumorigenic impact with the aid of immunocompetent mice. Colony formation, flow cytometry, western blot, ELISA, and qRT-PCR are used to elucidate mechanisms underlying EA11-led effects. Results. HS11 presents the most potential suppression of 4T1 cell proliferation and tumor growth among these aqueous extracts. The comparison results show that EA11 is more effective than HS11 in vitro and in vivo. EA11 inhibits colony formation and induces apoptosis in a concentration-dependent manner. EA11 reduces the protein expressions of PDE7B, PD-L1, β-catenin, and cyclin D1 while elevating the concentration of cellular cAMP and miR-200c expression in 4T1 cells. Additionally, EA11 exerts its anticancer effect partially via the inactivation of MAPK and AKT signaling pathways. Conclusions. This study implicates that EA11 prevents breast tumor development by interfering with the miR-200c-PDE7B/PD-L1-AKT/MAPK axis. EA11 may represent a potential therapeutic candidate for breast cancer.

scholarly journals IN VITRO SCREENING OF ANTIOXIDANT AND ANTIAGING POTENTIAL OF CUCUMIS SATIVUS FRUIT EXTRACT

2020 ◽  
pp. 187-190
Author(s):  
CHANCHAL GARG ◽  
RAVINDER SINGH ◽  
MUNISH GARG
Keyword(s):  
Ethyl Acetate ◽  
Cucumis Sativus ◽  
Inhibitory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Objective: The present study was designed to screen the anti-aging and anti-wrinkle potential of Cucumis sativus fruit through in vitro estimation of antioxidant, anti-hyaluronidase, anti-elastase, anti-collagenase/anti-matrix metalloproteinase (MMP)-1, and anti-tyrosinase activity. Methods: Raw juice of cucumber was taken, filtered and fractionated with ethyl acetate and n-butanol. The obtained extracts were then evaluated for their antioxidant potential through 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay taking ascorbic acid as positive control and other enzymatic activities in reference to hyaluronidase inhibition, MMP-1/collagenase inhibition, and elastase inhibition taking catechin as reference standard whereas for tyrosinase inhibition the standard used was quercetin. Results: All the evaluations were performed in triplicates and results were noted down. It was observed that aqueous extract of C. sativus fruits showed a maximum DPPH radical scavenging activity (p<0.0001), half-maximal inhibitory concentration (IC50) at a concentration of 122.67 μg/ml. The ethyl acetate fraction of C. sativus fruits exhibited maximum hyaluronidase (p<0.0001), MMP-1/collagenase (p<0.04), and tyrosinase (p<0.04) inhibitory activity, IC50 at a concentration of 59.54, 45.79, and 24.46 μg/ml, respectively. The elastase (p<0.0001) inhibitory activity by n-butanol fraction of C. sativus fruits extract was maximum, IC50 at a concentration of 52.76 μg/ml. Conclusion: A potent anti-aging and anti-wrinkle properties were well demonstrated by C. sativus, as depicted from the results obtained.

scholarly journals Evaluation of the antioxidant potentials of extract and fractions of Vitex doniana fruit and identification of the bioactive metabolites using HPLC-DAD-MS analysis

2021 ◽  
Vol 17 (1) ◽  
pp. 160-175
Author(s):  
Keziah Uchechi Ajah ◽  
Ozioma Anne Asomugha ◽  
Chinazom Perpetua Ifedigbo ◽  
Kosoluchukwu Chidubem Umeh ◽  
Onyeka Chinwuba Obidiegwu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Carbon Tetrachloride ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Fruit Extract ◽  
Ms Analysis ◽  
Vitex Doniana

Isoflavones and flavonoids in general found in fruits and vegetable act as natural antioxidants. This study was aimed at screening the isoflavone-rich fractions of Vitex doniana fruit extract for antioxidant activity using in vitro and in vivo models. The ethyl acetate and butanol fractions were screened for in vitro antioxidant activity by DPPH free radical scavenging and FRAP (Fe3+ Reducing Antioxidant Power) assays using ascorbic acid as standard. In vivo antioxidant activity was also tested against carbon tetrachloride-induced toxicity in mice using carbon tetrachloride (4 mL/kg) and silymarin (100 mg/kg) as standard. The ethyl acetate fraction and butanol Vacuum Liquid Chromatography (VLC) sub-fractions were subjected to High Performance Liquid Chromatography–Diode Array Detector (HPLC-DAD) and Liquid Chromatography-Mass Spectrometry (LC-MS) analyses. The ethyl acetate and butanol fractions inhibited DPPH radicals with an IC50 of 897 µg/mL and 2809.38 µg/mL respectively. Both fractions also showed mild FRAP result with EC50 of 1401 µg/mL and 7051 µg/mL respectively. The fractions produced significant decrease (P < 0.05) in ALP levels only while ethyl acetate fraction caused a significant reduction in the value of only malondialdehyde. Both fractions also increased levels of superoxide dismutase (SOD). HPLC-DAD analysis led to the detection of cinnamic acid, protocatechuic acid and two isoflavones daidzein and genistein. Further LC-MS analysis confirmed the presence of the isoflavones tectorigenin, 5-O-methylgenistein, and 5-O-methyltectorigenin, among other flavonoids, peonidin, 5-methylpeonidin, methylmalvidin and kaempferol-3-O-(2''galloyl)-glucopyranoside. The detected isoflavones and other flavonoids may contribute to the observed mild in vitro and good in vivo antioxidant activity of V. doniana fruit extract and fractions.

Antioxidant, anti-inflammatory and healing potential of ethyl acetate fraction of Bauhinia ungulata L. (Fabaceae) on in vitro and in vivo wound model

2020 ◽  
Vol 47 (4) ◽  
pp. 2845-2859
Author(s):  
Raphael de Oliveira Rodrigues ◽  
Juliana Navarro Ueda Yaochite ◽  
Greyce Luri Sasahara ◽  
Andressa Almeida Albuquerque ◽  
Said Gonçalves da Cruz Fonseca ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Wound Model ◽  
Anti Inflammatory

scholarly journals Antioxidant, Hepatoprotective, and Antidepression Effects of Rumex tingitanus Extracts and Identification of a Novel Bioactive Compound

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Dhekra Mhalla ◽  
Karama Zouari Bouassida ◽  
Rachid Chawech ◽  
Amira Bouaziz ◽  
Samar Makni ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Liver Toxicity ◽  
Bioactive Compound ◽  
Ethyl Acetate Fraction ◽  
Hydroalcoholic Extract ◽  
Immobility Time ◽  
Promising Source

Over the last few decades, Rumex species have been recognized as a promising source of new compounds with numerous pharmacological activities. Therefore, the antioxidant activity of Rumex tingitanus (R. tingitanus) leaves extracts was evaluated in vitro and then confirmed in vivo as well as the antidepressant-like and toxicological effects of the extracts. The ethyl acetate fraction (Rt EtOAcF) followed by hydroalcoholic extract (Rt EtOH-H2O) showed a remarkable in vitro antioxidant activity. The hydroalcoholic extract (Rt EtOH-H2O) showed significant hepatoprotective activity against carbon tetrachloride- (CCl4-) induced liver toxicity which is seen from inhibition of the malondialdehyde (MDA) accumulation and enhancement of the liver antioxidant enzymes activities. The Rt EtOH-H2O and Rt EtOAcF extracts were able to reduce the immobility time in mice and then elicited a significant antidepressant-like effect. The ethyl acetate fraction (Rt EtOAcF) was purified and resulted in the identification of a new antioxidant component called 4′-p-acetylcoumaroyl luteolin. The Rt EtOAcF and the 4′-p-acetylcoumaroyl luteolin revealed a strong antioxidant activity using DPPH test with IC50 of 11.7 ± 0.2 and 20.74 ± 0.6 μg/ml, respectively, and AAI of 3.39 and 1.92 better than that of BHT, used as control.

scholarly journals UJI IN VITRO DAN IN SILICO SENYAWA 5,7,2’,5’-TETRAHYDROXY FLAVAN-3-OL TERHADAP ENZIM ALPHA GLUCOSIDASE

2018 ◽  
Vol 5 (2) ◽  
pp. 279-283
Author(s):  
Frengki Frengki ◽  
Deddi Prima ◽  
Fatma Sri Wahyuni ◽  
Daan Khambri ◽  
Henny Vanda ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Ethyl Acetate ◽  
In Silico ◽  
Ethyl Acetate Fraction ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Inhibition Activity ◽  
Antidiabetic Effect ◽  
Alpha Glucosidase

Several of Calophyllums genus have been searched and proven as medicinal plants and Calophyllum macrophyllum is one of its genus. We have been isolated a compound from ethyl acetate fraction of the stem-bark. The compound was flavan-3-ol (5,7,2’,5’-tetrahydroxy flavan-3-ol). This research aimed to determine inhibition antidiabetic activity and affinity of its compound on α-glycosidase enzyme. In vitro antidiabetic effect shown by IC50 9.10 µg/ml and docking by Arguslab 4.01 shown by ΔG -10,24 kkal/mol as in silico test. The inhibition activity of flavan-3-ol against the α-glucosidase enzyme that is very strong shows its promising potential as a candidate for antidiabetic drugs.

scholarly journals Potensi Kulit Batang Bungur (Lagerstroemia loudonii Teijsm and Binn.) Sebagai Herbal Antidiabetes dengan Mekanisme Penghambat Alfa-glukosidase

2018 ◽  
Vol 1 (3) ◽  
pp. 117-120
Author(s):  
Soraya Riyanti ◽  
Julia Ratnawati ◽  
Muhammad Ibnu Shaleh ◽  
Asep Gana Suganda
Keyword(s):  
Ethyl Acetate ◽  
Colorimetric Method ◽  
In Vitro Study ◽  
Ethyl Acetate Fraction ◽  
Water Fraction ◽  
Ic50 Value ◽  
The Family ◽  
Ic50 Values ◽  
Alpha Glucosidase

Tumbuhan bungur (Lagerstroemia loudonii T.B.) termasuk dalam famili Lytrharceae. Famili Lythraceae telah diketahui memiliki aktivitas farmakologi sebagai antidiabetes, antiinflamasi, antimikroba, serta antiobesitas. Daun dan buah bungur (Lagerstroemia loudonii T.B.) memiliki aktivitas dalam menghambat alfa-glukosidase. Berdasarkan teori khemotaksonomi didalam tumbuhan, kemungkinan bagian lain dari tumbuhan bungur memiliki aktivitas dan kandungan kimia yang sama, sehingga dilakukan pengujian aktivitas penghambatan terhadap alfa-glukosidase pada bagian kulit batang bungur. Ekstraksi simplisia dilakukan dengan cara maserasi selama 24 jam menggunakan pelarut etanol 96%. Proses fraksinasi menggunakan cara Ekstraksi Cair-Cair (ECC) dengan pelarut n-heksana, etil asetat dan air. Pengujian aktivitas penghambatan alfa-glukosidase secara in vitro menggunakan metode kolorimetri dengan alat spektrofotometer UV-VIS pada panjang gelombang 400,4 nm dengan substrat p-nitrofenil-α-D-glukopiranosid (PNPG). Akarbose digunakan sebagai pembanding. Hasil penelitian menunjukkan bahwa ekstrak, fraksi air, fraksi etil asetat dan fraksi n-heksana memiliki nilai IC50 berturut-turut sebesar 240,53±0,47 μg/ml, 186,111±1,02 μg/ml, 79,479±0,52 μg/ml dan 113,101±0,46 μg/ml. Nilai IC50 akarbose adalah sebesar 10,457±1,48 μg/ml. Ekstrak dan fraksi-fraksi (air,etil asetat dan n-heksana) kulit batang bungur mampu menghambat aktivitas enzim α-glukosidase. Aktivitas yang paling baik ditunjukan oleh fraksi etil asetat dengan nilai IC50 sebesar 79,479±0,52 μg/ml.   Bungur (Lagerstroemia loudonii T.B.) is included in the Family  Lytrharceae. The Lythraceae has been known to have pharmacological activity as antidiabetic, anti-inflammatory, antimicrobial, and antiobesity. Leaves and fruits of bungur (Lagerstroemia loudonii T.B.) have activities to inhibit alpha-glucosidase. Based on the chemotaxonomy theory in plants, it is possible that other parts of the bungur plant have the same chemical activity and content, Hence the study to evalute the  inhibitory activity against alpha-glucosidase was carried out on its bark stem.The extraction of dried powder material was carried out by maceration for 24 hours using 96% ethanol. The extract was fractionated by Liquid-Liquid Extraction (ECC) method with n-hexane, ethyl acetate and water. The In vitro study of alpha-glucosidase inhibition activity using a colorimetric method with a UV-VIS spectrophotometer at a wavelength of 400.4 nm with a p-nitrophenyl-α-D-glucopiranoside (PNPG) substrate was performed. Akarbose was used as a standard drug.The results showed that extract, water fraction, ethyl acetate fraction and n-hexane fraction showed IC50 values ​​of 240.53 ± 0.47 μg / ml, 186.111 ± 1.02 μg / ml, 79.497 ± 0.52 μg/ ml and 113.101 ± 0.46 μg / ml, respectively. The IC50value of bungur was 10.457 ± 1.48 μg / ml. Extracts and fractions (water, ethyl acetate and n-hexane) of bark stem were able to inhibit the activity of α-glucosidase. Theethyl acetate fraction showed the strongestactivity with IC50 value of 79,479 ± 0.52 μg / ml

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