The antidepressant-like effects of the water extract of Panax ginseng and Polygala tenuifolia are mediated via the BDNF-TrkB signaling pathway and neurogenesis in the hippocampus

2021 ◽  
Vol 267 ◽  
pp. 113625
Author(s):  
Ning Jiang ◽  
Haixia Wang ◽  
Chenchen Li ◽  
Guirong Zeng ◽  
Jingwei Lv ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Panax Ginseng ◽  
Water Extract ◽  
Polygala Tenuifolia

scholarly journals Erigeron annuus (L.) Pers. Extract Inhibits Reactive Oxygen Species (ROS) Production and Fat Accumulation in 3T3-L1 Cells by Activating an AMP-Dependent Kinase Signaling Pathway

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 139 ◽  
Author(s):  
Yoon-Hee Choi ◽  
Ok-Hwan Lee ◽  
Yulong Zheng ◽  
Il-Jun Kang
Keyword(s):  
Reactive Oxygen Species ◽  
Signaling Pathway ◽  
Water Extract ◽  
Reactive Oxygen ◽  
Dependent Manner ◽  
Ros Production ◽  
Oxygen Species ◽  
Fat Accumulation ◽  
Ampk Signaling ◽  
Erigeron Annuus

Obesity is one of the major public health problems in the world because it is implicated in metabolic syndromes, such as type 2 diabetes, hypertension, and cardiovascular diseases. The objective of this study was to investigate whether Erigeron annuus (L.) Pers. (EAP) extract suppresses reactive oxygen species (ROS) production and fat accumulation in 3T3-L1 cells by activating an AMP-dependent kinase (AMPK) signaling pathway. Our results showed that EAP water extract significantly inhibits ROS production, adipogenesis, and lipogenesis during differentiation of 3T3-L1 preadipocytes. In addition, EAP decreased mRNA and protein levels of proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα). Moreover, EAP suppressed mRNA expressions of fatty acid synthase (FAS), lipoprotein lipase (LPL), adipocyte protein 2 (aP2) in a dose-dependent manner. Whereas, EAP upregulated adiponectin expression, phosphorylation levels of AMPK and carnitine palmitoyltransferase 1 (CPT-1) protein level during differentiation of 3T3-L1 preadipocytes. These results suggest that EAP water extract can exert ROS-linked anti-obesity effect through the mechanism that might involve inhibition of ROS production, adipogenesis and lipogenesis via an activating AMPK signaling pathway.

Biological active components found in panax ginseng improve glucose uptake via AMPK signaling pathway

2008 ◽  
Vol 22 (S2) ◽  
pp. 683-683 ◽  
Author(s):  
Jin Taek Hwang ◽  
MyoungSu Lee ◽  
MyungSunny Kim ◽  
Dae Young Kwon
Keyword(s):  
Glucose Uptake ◽  
Signaling Pathway ◽  
Panax Ginseng ◽  
Active Components ◽  
Ampk Signaling

scholarly journals A material-basis study of Aloe vera on the wnt/β-catenin signaling pathway using a knockin/knockout method with high-speed countercurrent chromatography

RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 38819-38829 ◽  
Author(s):  
Cong Dai ◽  
Meng-ping Liu ◽  
Wei-jia Zhang ◽  
Christopher Wai Kei Lam ◽  
Jian-ru Guo ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
High Speed ◽  
Target Genes ◽  
Aloe Vera ◽  
Water Extract ◽  
Hek293 Cells ◽  
Countercurrent Chromatography ◽  
Low Concentration ◽  
Material Basis

The water extract ofAloe veraat low concentration could activate the wnt/β-catenin signaling pathway and up-regulated the level of active β-catenin protein in hek293 cells, as well as promoting the expression of wnt target genes.

scholarly journals Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Hui Zhang ◽  
Yongbing Wang ◽  
Jiayang Liu ◽  
Kudelaidi Kuerban ◽  
Jian Li ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Flow Cytometry ◽  
Cell Death ◽  
Alternative Medicine ◽  
Signaling Pathway ◽  
Water Extract ◽  
Underlying Mechanism ◽  
Autophagic Cell Death ◽  
Therapeutic Effects ◽  
Traditional Uyghur Medicine

Abstract Background The water extract of Quercuse infectoria galls (QIG) is the active ingredient of Uyghur medicine Xipayi Kui Jie’an (KJA) which has promising therapeutic effects on Ulcerative Colitis (UC) as an alternative medicine. Considering the relationship between UC and the development of colorectal cancer (CRC), the present work aims to explore the direct anti-CRC activity of QIG extract. Methods CCK8 assay and flow cytometry were used to detect cytotoxicity and apoptosis. Transmission electron microscopy (TEM), flow cytometry, laser confocal and western blotting were performed to examine autophagy. We also adopted Reactive Oxygen Assay kit, as well as transwell and wound healing tests to study the underlying mechanism of QIG against CRC cells. Results First, we found that QIG extract could suppress the viability of CRC cells and trigger caspases-dependent apoptosis. Subsequently, we proved for the first time that QIG extract also triggered autophagic cell death in CRC cells, which together with apoptosis contributed to the cytotoxic effect on CRC cells. Further investigation revealed that QIG-induced cytotoxicity associated with intracellular ROS accumulation which could suppress the AKT/mTOR signaling pathway, and then induce autophagy and inhibit cell growth. Besides, Erk signaling pathway was also involved in the process of autophagic cell death. Moreover, QIG extract also influenced EMT process and inhibited CRC cell migration. Conclusion Altogether, this study provides a basis for the utilization of QIG as an alternative medicine for CRC prevention and treatment.

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