Antioxidant bioactive compounds in tomato fruits at different ripening stages and their effects on normal and cancer cells

2015 ◽  
Vol 18 ◽  
pp. 83-94 ◽  
Author(s):  
Rita Del Giudice ◽  
Assunta Raiola ◽  
Gian Carlo Tenore ◽  
Luigi Frusciante ◽  
Amalia Barone ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Bioactive Compounds ◽  
Tomato Fruits ◽  
Ripening Stages

Characterization and oxidative stability of oils and bioactive compounds of the fruits of Byrsonima cydoniifolia A. Juss. at different ripening stages

2019 ◽  
Vol 99 (6) ◽  
pp. 2855-2864 ◽  
Author(s):  
Gabriela Marcelino ◽  
Juliana R Donadon ◽  
Anderson RL Caires ◽  
Flavio S Michels ◽  
Lincoln CS Oliveira ◽  
...  
Keyword(s):  
Oxidative Stability ◽  
Bioactive Compounds ◽  
Ripening Stages

scholarly journals Biofortification with selenium increases bioactive compounds and antioxidant capacity in tomato fruits

2021 ◽  
Vol 39 ◽  
Author(s):  
Pablo Preciado-Rangel ◽  
Luis Guillermo Hernández-Montiel ◽  
Ricardo David Valdez-Cepeda ◽  
Efraín De la Cruz-Lázaro ◽  
Liliana Lara-Capistrán ◽  
...  
Keyword(s):  
Public Health ◽  
Bioactive Compounds ◽  
Nutritional Quality ◽  
Sodium Selenite ◽  
Human Consumption ◽  
Human Populations ◽  
Tomato Fruits ◽  
Nutritive Solution ◽  
Tomato Yield ◽  
Phytochemical Compounds

The objective of biofortification is the human consumption of high nutritional quality food, rich in micronutrients. Selenium (Se) is an essential micronutrient in human nutrition, and its essentiality has not been evidenced in plants. However, its application in crops and subsequent consumption can mitigate the deficiency of this micronutrient in the diet of human populations. This work analyzes the capacity of sodium selenite (Na2SeO3) to increase yield, biosynthesis of bioactive compounds and their accumulation in tomato fruits. For this, five treatments were applied via nutrient solution: 0, 2, 4, 6, and 8 mg L-1. At harvest, the nutraceutical quality and the accumulation of Se in fruits were quantified, as well as the productivity of tomato plant. Biofortification was positively affected by the biosynthesis of phytochemical compounds and their concentration in fruit, although tomato yield decreased. The incorporation of Se in nutritive solution is an alternative to increase both the biosynthesis of phytochemical compounds and the concentration of this element in tomato fruits with the possibility of improving public health through its consumption.

Bioactive compounds of fruit parts of three Eugenia uniflora biotypes in four ripening stages

2021 ◽  
Author(s):  
SUZANA C. SANTOS ◽  
Marx O. A. Pereira ◽  
Karoline B. Santos ◽  
Pedro H. Ferri
Keyword(s):  
Bioactive Compounds ◽  
Eugenia Uniflora ◽  
Ripening Stages

scholarly journals Pharmacology Evaluation of Bioactive Compounds that Regulate Cervical Cancer Cells

2019 ◽  
Author(s):  
Mauricio Salinas-Santander ◽  
Patricia Alvarez-Ortiz ◽  
Juan Alberto-Ascacio Valdes ◽  
Raul Rodriguez-Herrera ◽  
Alejandro Zugasti-Cruz ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Bioactive Compounds ◽  
Cervical Cancer Cells

scholarly journals Increased Oxidative Stress Induced by Rubus Bioactive Compounds Induce Apoptotic Cell Death in Human Breast Cancer Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-18 ◽  
Author(s):  
Blassan P. George ◽  
Heidi Abrahamse
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cell Death ◽  
Cytochrome C ◽  
Cancer Cells ◽  
Bioactive Compounds ◽  
Breast Cancer Cells ◽  
Apoptotic Cell ◽  
Ros Production ◽  
Caspase 9

Bioactive compounds from plants represent good candidate drugs for the prevention and treatment of various forms of cancer. Berries are rich sources of bioactive compounds, and there has been an increasing interest in the study of therapeutic action of wild berries. Oxidants are generated continuously in biological system as a result of physiological process. When there is an imbalance between oxidants and antioxidants, it leads to a condition called oxidative stress. Natural compounds as inducers of oxidative stress are able to modulate the physiological functions of cancer cells leading to cell death or survival. The aim of this study was to evaluate the induction of apoptosis by isolated bioactive compounds (1-(2-hydroxyphenyl)-4-methylpentan-1-one (C1) and 2-[(3-methylbutoxy) carbonyl] benzoic acid (C2)) from Rubus fairholmianus against MCF-7 breast cancer cells. The exposure of C1 and C2 reduced viability (IC50 of C1: 4.69; C2: 8.36 μg/mL) and proliferation. Cytochrome c release from mitochondria and changes in mitochondrial membrane potential of treated cells supported the intrinsic apoptotic cell death. Reactive oxygen species (ROS) production after treatment with C1 and C2 was found to be higher and induced nuclear damage. Expression of apoptotic proteins after the treatments was significantly upregulated as indicated using immunofluorescence (caspase 9, p53, and Bax), western blotting (p53, cleaved PARP, cytochrome c, and Bax), and ELISA (caspase 9) analysis. Overall, C1 was more cytotoxic, increased the ROS production in dichlorodihydrofluorescein diacetate assay, and induced apoptosis in breast cancer cells. These results illustrate that berry bioactive compounds have strong chemopreventive potential. In this article, we provide information on prooxidant and anticancer activities of Rubus bioactive compounds. Natural products have always demonstrated a significant contribution to the development of several cancer chemotherapeutic drugs. Most of these compounds are known to affect the redox state of the cell; and studies on these compounds have focused on their antioxidant property instead of prooxidant properties.

scholarly journals The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells

2019 ◽  
Vol 11 (1) ◽  
pp. 32-38
Author(s):  
Arif Rahman Hikam ◽  
Nuraeni Ekowati ◽  
Hernayanti Hernayanti
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Bioactive Compounds ◽  
Edible Mushroom ◽  
Chloroform Extract ◽  
Double Staining ◽  
Auricularia Auricula ◽  
Cervical Cancer Cells ◽  
Double Staining Method

Auricularia auricula is an edible mushroom cultivated in Indonesia that has been known to have potential properties of bioactive compounds than can be used for medicinal purpose. This study aimed to examine the cytotoxic and apoptosis effect of chloroform extract of A. auricula on cervical cancer cells in vitro. The research design was in vitro experimental research. Cytotoxic tests was using an MTT [3-(4,5-dimetiltiazol-2-yl)-2.5-diphenyl tertrazolium bromide] assay and apoptosis test was using double staining method. Test of bioactive compounds was carried out using GCMS. Cytotoxic effect were analyzed by linear regression and apoptosis test was analyzed descriptively. Chloroform extract of mycelium A. auricula showed the best results with IC50 = 264.87 µg/ml. An important finding obtained after the double staining process was that chloroform extract of A. auricula can induce HeLa cells death by apoptosis. GCMS test results showed that the extracts containing limonene and piperidinone which are the anticancer bioactive compounds. In conclusion, the chloroform extracts of A. auricula has the potential to inhibit the growth of cervical cancer cells. The benefit of this study are expected to provide information about the anticancer potential of extract of A. auricula against cervical cancer cells, thus contributing to the development of alternative anticancer treatments from natural product.

Hypoglycemic effect and bioactive compounds associated with the ripening stages of the Cucurbita ficifolia Bouché fruit

2020 ◽  
Vol 100 (14) ◽  
pp. 5171-5181
Author(s):  
Araceli Moya‐Hernández ◽  
Elsa Bosquez‐Molina ◽  
José Ramón Verde‐Calvo ◽  
Gerardo Blancas‐Flores ◽  
Gloria Maribel Trejo‐Aguilar
Keyword(s):  
Bioactive Compounds ◽  
Hypoglycemic Effect ◽  
Ripening Stages ◽  
Cucurbita Ficifolia

scholarly journals GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Rawan Al-Nemari ◽  
Abdulrahman Al-Senaidy ◽  
Abdelhabib Semlali ◽  
Mohammad Ismael ◽  
Ahmed Yacine Badjah-Hadj-Ahmed ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Bioactive Compounds ◽  
Wound Repair ◽  
Gas Chromatography Mass Spectrometry ◽  
Nuclear Staining ◽  
Colon Cancer Cells ◽  
Annona Squamosa ◽  
Anticancer Activities ◽  
Alternative Source

Abstract Background The research and application of plants in food supplements and drugs have attracted great interest. This study aimed to examine the efficiency of several solvents for the extraction of the main compounds from Annona squamosa leaves and to evaluate the antioxidant, antibacterial, and anticancer activities of these extracts. Methods Gas chromatography-mass spectrometry was used to screen the bioactive compounds of A. squamosa methanolic extract. The free radical, hydrogen peroxide, and nitric oxide scavenging activities of the extracts were investigated. Furthermore, MTT, nuclear staining, LDH, and monolayer wound repair assays were performed to evaluate the potential anticancer activity of the extracts in colon cancer cells while the antibacterial activity was tested by using a well diffusion assay. Results A. squamosa leaves extracts were found to contain several bioactive compounds, of which the majority were sesquiterpenes (C15H24). These extracts exhibited strong antioxidant activity and antibacterial potency against both gram-positive and gram-negative bacteria. Different A. squamosa leaves extracts displayed remarkable antiproliferative, cytotoxic, antimigration, and apoptotic activities in colon cancer cells. Conclusions A. squamosa leaves contain major bioactive compounds that inhibit the growth of several types of bacteria and colon cancer cell lines, which demonstrated their efficacy as an alternative source of antibiotics and for the development of novel drugs for colon cancer therapy.

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