Degradation of fibres from fruit by-products allows selective modulation of the gut bacteria in an in vitro model of the proximal colon

Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities. We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2). Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively. In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.

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Bifidobacterium longum D2 enhances microbial degradation of long-chain arabinoxylans in an in vitro model of the proximal colon

Beneficial Microbes ◽

10.3920/bm2015.0023 ◽

2015 ◽

Vol 6 (6) ◽

pp. 849-860 ◽

Cited By ~ 7

Author(s):

P. Truchado ◽

P. Van den Abbeele ◽

A. Rivière ◽

S. Possemiers ◽

L. De Vuyst ◽

...

Keyword(s):

Enzyme Activities ◽

Bacterial Communities ◽

In Vitro Model ◽

Bifidobacterium Longum ◽

Proximal Colon ◽

Sequencing Analysis ◽

Degrading Enzyme ◽

Vitro Model ◽

Long Chain

Long-chain arabinoxylans (LC-AX) are degraded in the colon by intestinal bacteria possessing AX-degrading enzymes, such as bifidobacteria. Enzymatic activity of intestinal bacterial might vary depending on the composition of the gut microbiota. To compare the enzymatic activities of the bacterial gut communities of two healthy individuals (donors D1 and D2), these bacterial communities were inoculated into in vitro model M-SHIME®. Differences in xylanase activities and denaturing gradient gel electrophoresis profiles, in particular a DNA-band corresponding with Bifidobacterium longum, were found in the proximal colon vessel. 16S rRNA gene sequencing analysis demonstrated the presence of two different B. longum species in these bacterial communities, showing 99% gene sequence similarity with B. longum NCC2705 and B. longum. subsp. longum KACC 91563, respectively, further referred to as B. longum D1 and B. longum D2. When grown on LC-AX as the sole added energy source, B. longum D2 displayed significantly higher activities of β-xylanase (5.3-fold), β-xylosidase (2.9-fold), and α-arabinofuranosidase (1.5-fold), respectively, compared to B. longum D1. When B. longum D2 was inoculated in the M-SHIME, inoculated with the bacterial gut communities of the individual with low AX-degrading enzyme activities, the β-xylanase activity increased (1.5-fold) in the proximal vessel. We demonstrated the presence of differences in LC-AX degrading enzyme activities of the bacterial gut communities of two individuals in the in vitro M-SHIME model, which could be linked to the presence of a potent AX-degrading B. longum (D2) strain.

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Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products: In vitro model

Journal of Cereal Science ◽

10.1016/j.jcs.2019.04.009 ◽

2019 ◽

Vol 87 ◽

pp. 325-333 ◽

Cited By ~ 3

Author(s):

Paweł Paśko ◽

Małgorzata Tyszka-Czochara ◽

Jacek Namieśnik ◽

Zenon Jastrzębski ◽

Hanna Leontowicz ◽

...

Keyword(s):

In Vitro Model ◽

Gluten Free ◽

Binding Properties ◽

Vitro Model ◽

By Products

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In vitro evaluation of the efficacy of peach stones as mycotoxin binders

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1324287l ◽

2013 ◽

pp. 287-296 ◽

Cited By ~ 2

Author(s):

Zorica Lopicic ◽

Aleksandra Bocarov-Stancic ◽

Mirjana Stojanovic ◽

Jelena Milojkovic ◽

Vladimir Pantic ◽

...

Keyword(s):

In Vitro Model ◽

Ph Value ◽

Raw Materials ◽

Mass Ratio ◽

Acid Modification ◽

Vitro Model ◽

Shell Particles ◽

Layer Chromatography ◽

By Products

The paper describes in vitro model for the evaluation of ability of peach shell (unmodified and modified), prepared at the Institute for Technology of Nuclear and Other Mineral Raw Materials, Belgrade, to adsorb different mycotoxins. Peach stones were obtained from ?Vino Zupa? Company from Aleksandrovac, where they have been disposed of as by-products from their Juice Factory. After proper preparation, two sorts of peach shell particles were used: one as unmodified peach shell particles (PS) and another one obtained by acid modification, denoted as MPS. Adsorption of six mycotoxins: aflatoxin B1 (AFL), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZON), diacetoxyscirpenol (DAS) and T-2 toxin by PS and MPS was tested in vitro. Crude extracts of mycotoxins, produced at the Department of Microbiology of Bio-ecological Center, Zrenjanin, were used for adsorption experiments. The ability of binding mycotoxins was evaluated in the electrolyte 0.1 M K2HPO4, which pH value was adjusted to 3.0 and 7.0, respectively. Mass ratio of individual mycotoxin and peach shell samples was 1:5000. The experimental mixtures were incubated for 1 hour on a rotary shaker (185 rpm) at room temperature (22-25?C). After incubation, the extractions of non-adsorbed mycotoxins from the filtrates were performed with organic solvents, and their quantification was done by thin-layer chromatography (TLC).

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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