Intestinal Lactobacillus species improve immune enhancement by ginsenoside Rb1 and ginsenoside Rb1 increases intestinal Akkermansia proportions

Abstract Lighvan cheese (Lighvan panir) is among the most famous traditional cheese in Iran for its desired aroma and flavor. Undoubtedly, the lactic acid bacteria especially the genus Lactobacillus are the critical factors in developing the aroma, flavor, and texture in Lighvan cheese. In this study, the Lactobacillus population of the main Lighvan cheese was investigated. The Lactobacillus of the main Lighvan cheese was isolated using specific culture methods according to previously published Guidelines. Then, the phylogenetic features were investigated and the phenotypic characteristics were examined using specific culture methods. Twenty-eight Gram-positive bacterial species were identified belonged to the genus Lactobacillus. According to the same sequences as each other, three groups (A, B, and C) of isolates were categorized with a high degree of similarity to L. fermentum (100%) and L. casei group (L. casei, L. paracasei, and L. rhamnosus) (99.0 to 100%). Random amplified polymorphic DNA (RAPD) fingerprint analysis manifested the presence of three clusters that were dominant in traditional Lighvan cheese. Cluster І was divided into 4 sub-clusters. By the result of carbohydrate fermentation pattern and 16S rRNA sequencing, isolates were identified as L. rhamnosus. The isolates in clusters II and III represented L. paracasei and L. fermentum, respectively as they were identified by 16S rRNA sequencing and fermented carbohydrate patterns. Our result indicated that the specific aroma and flavor of traditional Lighvan cheese can be related to its Lactobacillus population including L. fermentum, L. casei, L. paracasei, and L. rhamnosus. Graphical abstract

Download Full-text

Expression Of Concern: ‘Protective effects of ginsenoside Rb1 on H2O2-induced oxidative injury in human endothelial cell line (EA.hy926) via miR-210’

International Journal of Immunopathology and Pharmacology ◽

10.1177/20587384211000848 ◽

2021 ◽

Vol 35 ◽

pp. 205873842110008

Keyword(s):

Endothelial Cell ◽

Cell Line ◽

Oxidative Injury ◽

Human Endothelial Cell ◽

Protective Effects ◽

Ginsenoside Rb1 ◽

Endothelial Cell Line ◽

Human Endothelial Cell Line

Download Full-text

Probiotic Properties of Lactobacillus Species Isolated from Fermented Palm Sap in Thailand

Probiotics and Antimicrobial Proteins ◽

10.1007/s12602-021-09754-y ◽

2021 ◽

Author(s):

Phoomjai Sornsenee ◽

Kamonnut Singkhamanan ◽

Surasak Sangkhathat ◽

Phanvasri Saengsuwan ◽

Chonticha Romyasamit

Keyword(s):

Lactobacillus Species ◽

Probiotic Properties

Download Full-text

Ginsenoside Rb1 protects from Staphylococcus aureus-induced oxidative damage and apoptosis through endoplasmic reticulum-stress and death receptor-mediated pathways

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2021.112353 ◽

2021 ◽

Vol 219 ◽

pp. 112353

Author(s):

Aftab Shaukat ◽

Irfan Shaukat ◽

Shahid Ali Rajput ◽

Rizwan Shukat ◽

Sana Hanif ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Endoplasmic Reticulum ◽

Endoplasmic Reticulum Stress ◽

Oxidative Damage ◽

Death Receptor ◽

Ginsenoside Rb1

Download Full-text

Modulation of Calcium Signaling of Angiotensin AT1, Endothelin ETA, and ETB Receptors by Silibinin, Quercetin, Crocin, Diallyl Sulfides, and Ginsenoside Rb1

Planta Medica ◽

10.1055/s-0034-1383408 ◽

2014 ◽

Vol 81 (08) ◽

pp. 670-678 ◽

Cited By ~ 3

Author(s):

Ruba Bahem ◽

Anja Hoffmann ◽

Arnaud Azonpi ◽

Catherina Caballero-George ◽

Patrick Vanderheyden

Keyword(s):

Calcium Signaling ◽

Ginsenoside Rb1

Download Full-text

Immune enhancement effects of Korean ginseng berry polysaccharides on RAW264.7 macrophages through MAPK and NF-κB signalling pathways

Food and Agricultural Immunology ◽

10.1080/09540105.2021.1934419 ◽

2021 ◽

Vol 32 (1) ◽

pp. 298-309

Author(s):

Weerawan Rod-in ◽

Natchanok Talapphet ◽

Chaiwat Monmai ◽

A.-yeong Jang ◽

SangGuan You ◽

...

Keyword(s):

Signalling Pathways ◽

Raw264.7 Macrophages ◽

Immune Enhancement ◽

Korean Ginseng

Download Full-text

Ginsenoside Rb1 Treatment Attenuates Pulmonary Inflammatory Cytokine Release and Tissue Injury following Intestinal Ischemia Reperfusion Injury in Mice

Oxidative Medicine and Cellular Longevity ◽

10.1155/2015/843721 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 22

Author(s):

Ying Jiang ◽

Zhen Zhou ◽

Qing-tao Meng ◽

Qian Sun ◽

Wating Su ◽

...

Keyword(s):

Lung Injury ◽

Signaling Pathway ◽

Intestinal Ischemia ◽

Ischemia Reperfusion ◽

Critical Role ◽

Weight Ratio ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Ginsenoside Rb1 ◽

Intestinal Ischemia Reperfusion

Objective. Intestinal ischemia reperfusion (II/R) injury plays a critical role in remote organ dysfunction, such as lung injury, which is associated with nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. In the present study, we tested whether ginsenoside Rb1 attenuated II/R induced lung injury by Nrf2/HO-1 pathway.Methods. II/R injury was induced in male C57BL/6J mice by 45 min of superior mesenteric artery (SMA) occlusion followed by 2 hours of reperfusion. Ginsenoside Rb1 was administrated prior to reperfusion with or without ATRA (all-transretinoic acid, the inhibitor of Nrf2/ARE signaling pathway) administration before II/R.Results. II/R induced lung histological injury, which is accompanied with increased levels of malondialdehyde (MDA), interleukin- (IL-) 6, and tumor necrosis factor- (TNF-)αbut decreased levels of superoxide dismutase (SOD) and IL-10 in the lung tissues. Ginsenoside Rb1 reduced lung histological injury and the levels of TNF-αand MDA, as well as wet/dry weight ratio. Interestingly, the increased Nrf2 and HO-1 expression induced by II/R in the lung tissues was promoted by ginsenoside Rb1 treatment. All these changes could be inhibited or prevented by ATRA.Conclusion. Ginsenoside Rb1 is capable of ameliorating II/R induced lung injuries by activating Nrf2/HO-1 pathway.

Download Full-text

Cytokine profiles and Lactobacillus species presence in pre-menopausal subjects with genital Mycoplasma genitalium or Ureaplasma urealyticum colonization

Women s Health ◽

10.1177/17455065211009181 ◽

2021 ◽

Vol 17 ◽

pp. 174550652110091

Author(s):

John Garza ◽

Kushal Gandhi ◽

Sarah Choi ◽

Asley Sanchez ◽

Gary Ventolini

Keyword(s):

Ureaplasma Urealyticum ◽

Mycoplasma Genitalium ◽

Interleukin 12 ◽

Lactobacillus Species ◽

Control Group ◽

Study Group ◽

Species Dominance ◽

Cytokine Profiles ◽

Menopausal Women ◽

Significant Difference

Background and Purpose: Lactobacilli play a vital role in protecting the vagina against pathogens. Cytokines are vital components of defense against infections in women. The genital mycoplasmas, Mycoplasma genitalium and Ureaplasma urealyticum, are associated with various infectious diseases in adults and infants. The objective of our study is to identify differences in cytokine profile and Lactobacillus species dominance between a study group of non-pregnant pre-menopausal women with genital M. genitalium or U. urealyticum colonization and a control group of non-pregnant pre-menopausal women without genital M. genitalium or U. urealyticum colonization. Methods: A real-time polymerase chain reaction was performed to measure Lactobacillus species in vaginal swab samples. Cytokine analysis was performed using multiplex immunoassay techniques. Analysis of variance confirmed a significant difference in cytokine profiles between patient groups, with t-tests identifying the most significantly different cytokines. Categorical data analysis identified significant patterns of relative Lactobacillus species dominance in the study group. Results: Lactobacillus iners was the predominant Lactobacillus species in the control group ( p = 0.005). There were no dominant Lactobacillus species observed in the study group. Vascular endothelial growth factor A ( p = 0.002), interleukin-8 ( p = 0.001), and interleukin-1β ( p = 0.049) were expressed significantly higher in the study group, whereas interleukin-1 receptor antagonist ( p < 0.001), interleukin-10 ( p = 0.001), interleukin-12 ( p = 0.002), and interferon-γ ( p = 0.022) were expressed higher in the control group. Association matrices for cytokines were significantly different between two groups ( p < 0.001), with mostly negative associations in the control group and mostly positive associations in the study group. Conclusion: Cytokine levels, their associations, and the patterns of Lactobacillus species dominance are observed to significantly diverge on the basis of M. genitalium and U. urealyticum colonization among non-pregnant pre-menopausal women.

Download Full-text