High intensity pulsed electric fields and heat treatments applied to a protease from Bacillus subtilis. A comparison study of multiple systems

2005 ◽  
Vol 69 (3) ◽  
pp. 317-323 ◽  
Author(s):  
Sílvia Bendicho ◽  
A. Robert Marsellés-Fontanet ◽  
Gustavo V. Barbosa-Cánovas ◽  
Olga Martín-Belloso
2009 ◽  
Vol 89 (15) ◽  
pp. 2636-2642 ◽  
Author(s):  
Luciano José Quitão-Teixeira ◽  
Isabel Odriozola-Serrano ◽  
Robert Soliva-Fortuny ◽  
Afonso Mota-Ramos ◽  
Olga Martín-Belloso

Food Control ◽  
2011 ◽  
Vol 22 (12) ◽  
pp. 1975-1983 ◽  
Author(s):  
M. Morales-de la Peña ◽  
L. Salvia-Trujillo ◽  
M.A. Rojas-Graü ◽  
O. Martín-Belloso

LWT ◽  
2009 ◽  
Vol 42 (4) ◽  
pp. 813-818 ◽  
Author(s):  
Ingrid Aguiló-Aguayo ◽  
Gemma Oms-Oliu ◽  
Robert Soliva-Fortuny ◽  
Olga Martín-Belloso

1998 ◽  
Vol 4 (1) ◽  
pp. 33-44 ◽  
Author(s):  
R. Pagán ◽  
S. Esplugas ◽  
M.M. Góngora-Nieto ◽  
G.V. Barbosa-Cánovas ◽  
B.G. Swanson

The inactivation of Bacillus subtilis spores using high intensity pulsed electric fields (HIPEF) was investigated. Spores were not inactivated when HIPEF treatment (60 kV/cm, 75 pulses) was used alone. The combination of-HIPEF and moderate temperatures around 60°C, and/or the activation of spore suspension prior to HIPEF treatment, and/or the use of up to 5000 IU/ml lysozyme, did not inactivate spores. High hydrostatic pressure (1500 atm, 30 min, 40°C) resulted in the initiation of germination of more than five log cycles in the number of spores, making them sensitive to subsequent pasteurization heat treatment, whereas they were not sensitive to subsequent HIPEF treatment at temperatures less than 40 °C. An intermediate step is needed which allows the outgrowth of spores to vegetative cells. Thus, the combination of high hydrostatic pressure and HIPEF treatment offers an attractive alternative to the stabilization of food products by heat to inactivate spores.


2003 ◽  
Vol 17 (11) ◽  
pp. 1-23 ◽  
Author(s):  
Stephen J. Beebe ◽  
Paula M. Fox ◽  
Laura J. Rec ◽  
Lauren K. Willis ◽  
Karl H. Schoenbach

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