Changes in nitric oxide/hydrogen peroxide content and cell cycle progression: Study with synchronized cultures of green alga Chlamydomonas reinhardtii

2017 ◽  
Vol 208 ◽  
pp. 84-93 ◽  
Author(s):  
Wojciech Pokora ◽  
Anna Aksmann ◽  
Agnieszka Baścik-Remisiewicz ◽  
Agnieszka Dettlaff-Pokora ◽  
Max Rykaczewski ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Peroxide Content ◽  
Synchronized Cultures

scholarly journals PROCYCLIC Trypanosoma brucei CELL CYCLE is IMPAIRED at the G1 STAGE by the PRESENCE of POLY (ADP-RIBOSE) in the NUCLEUS

2018 ◽  
Author(s):  
Mariana Schlesinger ◽  
Salomé C. Vilchez Larrea ◽  
Silvia H. Fernández Villamil
Keyword(s):  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Flow Cytometry ◽  
Trypanosoma Brucei ◽  
Cell Cycle Progression ◽  
G1 Phase ◽  
Cycle Progression ◽  
Transgenic Parasites ◽  
First Time ◽  
Synchronized Cultures

AbstractPreviously we demonstrated that an excess of poly (ADP-ribose) in the nucleus makes procyclic parasites more sensitive to hydrogen peroxide. However, the effect of an altered-PAR metabolism under standard conditions has not been addressed yet. Here we have analyzed the behavior of the growth curve of transgenic parasites that present this phenotype and studied cell cycle progression in synchronized cultures by flow cytometry and immunofluorescence. We have demonstrated that an excess of nuclear poly (ADP-ribose) produces a delay in the G1 phase of the cell cycle. Moreover, for the first time we have shown that poly (ADP-ribose) occurs at specific points very close to the mature basal body, suggesting there could be a link between the kinetoplast and poly (ADP-ribose) metabolism.

scholarly journals Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 735 ◽  
Author(s):  
Mária Čížková ◽  
Monika Slavková ◽  
Milada Vítová ◽  
Vilém Zachleder ◽  
Kateřina Bišová
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Cell Cycle Progression ◽  
Kinase Activity ◽  
Cyclin Dependent Kinase ◽  
Cycle Progression ◽  
Mitotic Cyclin ◽  
Cell Cycle Block

DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the antibiotic zeocin, inducing double stranded DNA breaks, on the cell cycle of synchronized cultures of the green alga Chlamydomonas reinhardtii. After zeocin application, DNA replication partially occurred but nuclear and cellular divisions were completely blocked. Application of zeocin combined with caffeine, known to alleviate DNA checkpoints, decreased cell viability significantly. This was probably caused by a partial overcoming of the cell cycle progression block in such cells, leading to aberrant cell divisions. The cell cycle block was accompanied by high steady state levels of mitotic cyclin-dependent kinase activity. The data indicate that DNA damage response in C. reinhardtii is connected to the cell cycle block, accompanied by increased and stabilized mitotic cyclin-dependent kinase activity.

scholarly journals To Divide or Not to Divide? How Deuterium Affects Growth and Division of Chlamydomonas reinhardtii

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 861
Author(s):  
Veronika Kselíková ◽  
Vilém Zachleder ◽  
Kateřina Bišová
Keyword(s):  
Cell Cycle ◽  
Chlamydomonas Reinhardtii ◽  
Cell Cycle Progression ◽  
Model Organism ◽  
Cycle Progression ◽  
Synchronous Cultures ◽  
Multiple Fission ◽  
First Choice ◽  
High Doses

Extensive in vivo replacement of hydrogen by deuterium, a stable isotope of hydrogen, induces a distinct stress response, reduces cell growth and impairs cell division in various organisms. Microalgae, including Chlamydomonas reinhardtii, a well-established model organism in cell cycle studies, are no exception. Chlamydomonas reinhardtii, a green unicellular alga of the Chlorophyceae class, divides by multiple fission, grows autotrophically and can be synchronized by alternating light/dark regimes; this makes it a model of first choice to discriminate the effect of deuterium on growth and/or division. Here, we investigate the effects of high doses of deuterium on cell cycle progression in C. reinhardtii. Synchronous cultures of C. reinhardtii were cultivated in growth medium containing 70 or 90% D2O. We characterize specific deuterium-induced shifts in attainment of commitment points during growth and/or division of C. reinhardtii, contradicting the role of the “sizer” in regulating the cell cycle. Consequently, impaired cell cycle progression in deuterated cultures causes (over)accumulation of starch and lipids, suggesting a promising potential for microalgae to produce deuterated organic compounds.

scholarly journals DNA Damage during G2 Phase Does Not Affect Cell Cycle Progression of the Green Alga Scenedesmus quadricauda

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e19626 ◽  
Author(s):  
Monika Hlavová ◽  
Mária Čížková ◽  
Milada Vítová ◽  
Kateřina Bišová ◽  
Vilém Zachleder
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Green Alga ◽  
Cell Cycle Progression ◽  
Scenedesmus Quadricauda ◽  
Cycle Progression ◽  
Affect Cell Cycle Progression ◽  
G2 Phase ◽  
Alga Scenedesmus Quadricauda

QS421. Nitric Oxide Regulates the Ubiquitin-Conjugating Enzymes Crucial for Cell Cycle Progression

2009 ◽  
Vol 151 (2) ◽  
pp. 301
Author(s):  
N.D. Tsihlis ◽  
C.S. Oustwani ◽  
M.N. Banerjee ◽  
Q. Jiang ◽  
M.R. Kibbe
Keyword(s):  
Nitric Oxide ◽  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Conjugating Enzymes ◽  
Ubiquitin Conjugating Enzymes
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