In vitro comparison of probiotic properties of Lactobacillus casei Zhang, a potential new probiotic, with selected probiotic strains

LWT ◽  
2009 ◽  
Vol 42 (10) ◽  
pp. 1640-1646 ◽  
Author(s):  
Zhuang Guo ◽  
Jicheng Wang ◽  
Liya Yan ◽  
Wei Chen ◽  
Xiao-ming Liu ◽  
...  
Keyword(s):  
Lactobacillus Casei ◽  
Probiotic Properties ◽  
Probiotic Strains ◽  
Lactobacillus Casei Zhang ◽  
In Vitro Comparison

A pilot study on the effect of Lactobacillus casei Zhang on intestinal microbiota parameters in Chinese subjects of different age

2014 ◽  
Vol 5 (3) ◽  
pp. 295-304 ◽  
Author(s):  
L.Y. Kwok ◽  
L. Wang ◽  
J. Zhang ◽  
Z. Guo ◽  
H. Zhang
Keyword(s):  
Lactobacillus Casei ◽  
Age Groups ◽  
Short Chain Fatty Acids ◽  
Fermented Milk ◽  
In Vitro Tests ◽  
Probiotic Properties ◽  
Lactobacillus Casei Zhang ◽  
The Impact ◽  
Chinese Subjects

Ageing of the population is an imminent global problem. Lactobacillus casei Zhang (LcZ) was isolated from Inner Mongolian fermented milk, koumiss. LcZ possesses numerous probiotic properties in in vitro tests and in animal models. However, it has never been tested in any human trial. In the current study, the impact of oral consumption of LcZ on different age groups was tested. Chinese subjects, including 10 young, 7 middle-aged and 7 elderly volunteers (with mean age of 24.3, 47.6 and 64.7, respectively), were recruited. Each subject took 10.6 log10 cfu LcZ daily for a continuous period of 28 days. Several parameters, including the amounts of LcZ and four selected groups of bacteria, change of bacterial diversity, short chain fatty acids (SCFA) and total bile acids (TBA), were monitored in faecal samples collected from the subjects before starting, during and after stopping oral LcZ consumption. The consumption of LcZ exhibited beneficial effects to the subjects by modulating faecal microbiota in a temporal manner with a prolonged elevation of SCFA and reduction of TBA. The potentially harmful Pseudomonas and Acinetobacter genera were suppressed by the probiotic administration. Furthermore, a moderately divergent response was observed in the indigenous gut populations of Bifidobacterium and Bacteroides fragilis group in different age subjects. Taken together, the current study has provided proof on the positive effect of probiotic consumption and crucial insights into the design and application of probiotic-based products to users of different age segments.

Probiotic properties of native Lactobacillus spp. strains for dairy calves

2018 ◽  
Vol 9 (4) ◽  
pp. 613-624 ◽  
Author(s):  
S. Fernández ◽  
M. Fraga ◽  
E. Silveyra ◽  
A.N. Trombert ◽  
A. Rabaza ◽  
...  
Keyword(s):  
Lactobacillus Reuteri ◽  
Bacterial Species ◽  
Control Group ◽  
Probiotic Properties ◽  
Real Time Quantitative Pcr ◽  
Treatment And Prevention ◽  
Probiotic Strains ◽  
Lactobacillus Spp

The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves.

scholarly journals Lactobacillus rhamnosus GG Genomic and Phenotypic Stability in an Industrial Production Process

2020 ◽  
Vol 86 (6) ◽  
Author(s):  
Marianne Stage ◽  
Anita Wichmann ◽  
Mette Jørgensen ◽  
Natalia Ivonne Vera-Jimenéz ◽  
Malue Wielje ◽  
...  
Keyword(s):  
Production Process ◽  
Gene Cluster ◽  
Industrial Production ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Properties ◽  
Phenotypic Stability ◽  
Content Type ◽  
Freeze Dried ◽  
Probiotic Strains

ABSTRACT Lactobacillus rhamnosus GG is one of the most widely marketed and studied probiotic strains. In L. rhamnosus GG, the spaCBA-srtC1 gene cluster encodes pili, which are important for some of the probiotic properties of the strain. A previous study showed that the DNA sequence of the spaCBA-srtC1 gene cluster was not present in some L. rhamnosus GG variants isolated from liquid dairy products. To examine the stability of the L. rhamnosus GG genome in an industrial production process, we sequenced the genome of samples of L. rhamnosus GG (DSM 33156) collected at specific steps of the industrial production process, including the culture collection stock, intermediate fermentations, and final freeze-dried products. We found that the L. rhamnosus GG genome sequence was unchanged throughout the production process. Consequently, the spaCBA-srtC1 gene locus was intact and fully conserved in all 31 samples examined. In addition, different production batches of L. rhamnosus GG exhibited consistent phenotypes, including the presence of pili in final freeze-dried products, and consistent characteristics in in vitro assays of probiotic properties. Our data show that L. rhamnosus GG is highly stable in this industrial production process. IMPORTANCE Lactobacillus rhamnosus GG is one of the best-studied probiotic strains. One of the well-characterized features of the strain is the pili encoded by the spaCBA-srtC1 gene cluster. These pili are involved in persistence in the gastrointestinal tract and are important for the probiotic properties of L. rhamnosus GG. Previous studies demonstrated that the L. rhamnosus GG genome can be unstable under certain conditions and can lose the spaCBA-srtC1 gene cluster. Since in vitro studies have shown that the loss of the spaCBA-srtC1 gene cluster decreases certain L. rhamnosus GG probiotic properties, we assessed both the genomic stability and phenotypic properties of L. rhamnosus GG throughout an industrial production process. We found that neither genomic nor phenotypic changes occurred in the samples. Therefore, we demonstrate that L. rhamnosus GG retains the spaCBA-srtC1 cluster and exhibits excellent genomic and phenotypic stability in the specific industrial process examined here.

scholarly journals Isolation, identification and characterisation of three novel probiotic strains (Lactobacillus paracaseiCNCM I-4034,Bifidobacterium breveCNCM I-4035 andLactobacillus rhamnosusCNCM I-4036) from the faeces of exclusively breast-fed infants

2013 ◽  
Vol 109 (S2) ◽  
pp. S51-S62 ◽  
Author(s):  
Sergio Muñoz-Quezada ◽  
Empar Chenoll ◽  
José María Vieites ◽  
Salvador Genovés ◽  
José Maldonado ◽  
...  
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Strain Identification ◽  
Probiotic Properties ◽  
Probiotic Strains ◽  
High Concentrations ◽  
Different Strains ◽  
Breast Fed

The aim of the present study was to isolate, identify and characterise novel strains of lactic acid bacteria and bifidobacteria with probiotic properties from the faeces of exclusively breast-fed infants. Of the 4680 isolated colonies, 758 exhibited resistance to low pH and tolerance to high concentrations of bile salts; of these, only forty-two exhibited a strong ability to adhere to enterocytesin vitro.The identities of the isolates were confirmed by 16S ribosomal RNA (rRNA) sequencing, which permitted the grouping of the forty-two bacteria into three different strains that showed more than 99 % sequence identity withLactobacillus paracasei,Lactobacillus rhamnosusandBifidobacterium breve, respectively. The strain identification was confirmed by sequencing the 16S–23S rRNA intergenic spacer regions. Strains were assayed for enzymatic activity and carbohydrate utilisation, and they were deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) of the Institute Pasteur and namedL. paracaseiCNCM I-4034,B. breveCNCM I-4035 andL. rhamnosusCNCM I-4036. The strains were susceptible to antibiotics and did not produce undesirable metabolites, and their safety was assessed by acute ingestion in immunocompetent and immunosuppressed BALB/c mouse models. The three novel strains inhibitedin vitrothe meningitis aetiological agentListeria monocytogenesand human rotavirus infections.B. breveCNCM I-4035 led to a higher IgA concentration in faeces and plasma of mice. Overall, these results suggest thatL. paracaseiCNCM I-4034,B. breveCNCM I-4035 andL. rhamnosusCNCM I-4036 should be considered as probiotic strains, and their human health benefits should be further evaluated.

The effects of inulin and fructo-oligosaccharide on the probiotic properties of Lactobacillus spp. isolated from human milk

2018 ◽  
Vol 73 (9-10) ◽  
pp. 367-373 ◽  
Author(s):  
Şener Tulumoğlu ◽  
Belgin Erdem ◽  
Ömer Şimşek
Keyword(s):  
Human Milk ◽  
Bile Salt ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Antagonistic Activity ◽  
Probiotic Properties ◽  
Probiotic Strains ◽  
And Control ◽  
Lactobacillus Spp

Abstract This study aims to determine the effects of inulin and fructo-oligosaccharide (FOS) on the probiotic properties of five Lactobacillus spp. isolated from human milk. Lactobacillus spp. were isolated and identified, and the growth characteristics, acid and bile salt tolerance, antagonistic effects, and cholesterol assimilation of Lactobacillus strains were investigated in the presence of inulin and FOS. Lactobacillus casei L1 was able to utilize inulin and FOS as carbon source as well as glucose even other strains were able to use, including Lactobacillus rhamnosus GG. This strain also showed high tolerance to acid and bile salt, even at pH 2.5 and 0.5% bile salt levels, respectively. Inulin and FOS promoted the antimicrobial activity of L. casei L1 against pathogenic bacteria. Cholesterol assimilation was higher than in the other and control probiotic strains in the presence inulin and FOS, which were measured as 14 and 25 mg/dL, respectively. In conclusion, L. casei L1 can use both inulin and FOS to maintain its viability both at digestive conditions and also the relevant prebiotics, and show broad antagonistic activity and cholesterol assimilation.

scholarly journals THE UNEXPLORED ROLES OF PROBIOTIC BACTERIA: IN VITRO ANTI-INFLAMMATORY AND ANTHELMINTIC ACTIVITY OF ENTEROCOCCUS FAECIUM BM10 KY788342 AND LACTOBACILLUS CASEI GM10 KY794586

2017 ◽  
Vol 10 (9) ◽  
pp. 117
Author(s):  
Neha Jain ◽  
Archana Mehta
Keyword(s):  
Enterococcus Faecium ◽  
Maximum Concentration ◽  
Lactobacillus Casei ◽  
Protein Denaturation ◽  
Anthelmintic Activity ◽  
Inflammatory Activity ◽  
Probiotic Strains ◽  
Anti Inflammatory ◽  
Vitro Model

  Objective: The aim of the present study was to evaluate the in vitro anti-inflammation activity and anthelmintic potential of two novel isolated probiotic strains through Enterococcus faecium BM10 KY788342 and Lactobacillus casei GM10 KY794586.Methods: In vitro anti-inflammatory activity was evaluated using protein denaturation inhibition method. Pheretima posthuma was used as a suitable in vitro model, and time of paralysis and death were used as parameters to evaluate anthelmintic potential of probiotic strains.Results: Lyophilized solutions of L. casei GM10 showed significant protein denaturation inhibition (56.20±0.86%) followed by E. faecium BM10 (52.28±0.31%) comparable to diclofenac (93.62±1.39%) at the maximum concentration of 250 μg/ml. Intracellular cell-free extract of E. faecium BM10 showed a strong anthelmintic (vermicidal) activity (6±0.23 minutes), followed by L. casei GM10 (9±0.05 minutes) comparable to piperazine citrate (20±0.422 minutes) and albendazole (24±0.43 minutes) at the maximum concentration of 100 mg/ml.Conclusion: Results of the present study concluded that both tested lactic acid bacteria strains exhibited significant in vitro anti-inflammatory activity and can be used as potent and safe anthelmintic agent.

scholarly journals Metagenomic and metatranscriptomic profiling of Lactobacillus casei Zhang in the human gut

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jicheng Wang ◽  
Jiachao Zhang ◽  
Wenjun Liu ◽  
Heping Zhang ◽  
Zhihong Sun
Keyword(s):  
Lactobacillus Casei ◽  
Lactic Acid Production ◽  
Expression Patterns ◽  
Human Gut ◽  
Activation Patterns ◽  
Lactobacillus Casei Zhang ◽  
Probiotic Mechanisms ◽  
Abc Transporter Genes

AbstractLittle is known about the replication and dynamic transcription of probiotics during their “passenger” journey in the human GI tract, which has therefore limited the understanding of their probiotic mechanisms. Here, metagenomic and metatranscriptomic sequencing was used to expose the in vivo expression patterns of the probiotic Lactobacillus casei Zhang (LcZ), which was compared with its in vitro growth transcriptomes, as well as the dynamics of the indigenous microbiome response to probiotic consumption. Extraction of the strain-specific reads revealed that replication and transcripts from the ingested LcZ were increased, while those from the resident L. casei strains remained unchanged. Mapping of all sequencing reads to LcZ genome showed that gene expression in vitro and in vivo differed dramatically. Approximately 39% of mRNAs and 45% of sRNAs of LcZ well-expressed were repressed after ingestion into human gut. The expression of ABC transporter genes and amino acid metabolism genes was induced at day 14 of ingestion, and genes for sugar and SCFA metabolism were activated at day 28 of ingestion. Expression of rli28c sRNA with peaked expression during the in vitro stationary phase was also activated in the human gut; this sRNA repressed LcZ growth and lactic acid production in vitro. However, the response of the human gut microbiome to LcZ was limited and heterogeneous. These findings implicate the ingested probiotic has to change its transcription patterns to survive and adapt in the human gut, and the time-dependent activation patterns indicate highly dynamic cross-talk between the probiotic and human gut microbes.

scholarly journals ISOLATION, CLASSIFICATION AND IDENTIFICATION OF POTENTIAL PROBIOTIC LACTOBACILLUS STRAINS FROM INFANT FAECES

2011 ◽  
Vol 14 (4) ◽  
pp. 62-76
Author(s):  
Khanh Quoc Hoang ◽  
Thanh Thi Lan Pham
Keyword(s):  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Probiotic Properties ◽  
Specific Pcr ◽  
Probiotic Lactobacillus ◽  
Probiotic Strains ◽  
Pcr Method ◽  
Breast Fed ◽  
Rdna Analysis

Lactobacillus bacteria present in many probiotic products. This paper investigated probiotic Lactobacillus strains isolated from the human gastrointestinal tract. 15 Lactobacillus strains were isolated from breast-fed infant faeces and identified by both traditional methods and genus-specific PCR method. In vitro experiments were designed to investigate some probiotic properties such as resistance to low pH and bile, cell surface hydrophobicity, antimicrobial activity, bacteriocin and other antimicrobials production, antibiotic resistance and cholesterol reduction. As a result, 12 probiotic Lactobacillus strains were selected. Significantly, 11 strains of them reduced 10-33.34 % serum cholesterol level. By 16S rDNA analysis, the probiotic strains were classified at species level as Lactobacillus gasseri, L. fermentum, L. salivarius, L. rhamnosus and L. paracasei/ casei.

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