High genetic diversity in a single population of Triatoma sanguisuga (LeConte, 1855) inferred from two mitochondrial markers: Cytochrome b and 16S ribosomal DNA

2011 ◽  
Vol 11 (3) ◽  
pp. 671-677 ◽  
Author(s):  
N. de la Rua ◽  
L. Stevens ◽  
P.L. Dorn
Keyword(s):  
Genetic Diversity ◽  
Cytochrome B ◽  
Ribosomal Dna ◽  
Single Population ◽  
High Genetic Diversity ◽  
Mitochondrial Markers ◽  
16S Ribosomal Dna

scholarly journals Aggressiveness Changes in Populations of Didymella pinodes over Winter and Spring Pea Cropping Seasons

2016 ◽  
Vol 82 (14) ◽  
pp. 4330-4339 ◽  
Author(s):  
G. Laloi ◽  
J. Montarry ◽  
M. Guibert ◽  
D. Andrivon ◽  
D. Michot ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ascochyta Blight ◽  
Growing Season ◽  
Climatic Conditions ◽  
Single Population ◽  
High Genetic Diversity ◽  
Content Type ◽  
Didymella Pinodes ◽  
Pathogenicity Tests ◽  
Cropping Seasons

ABSTRACTAscochyta blight, caused by the necrotrophic ascomyceteDidymella pinodes, is responsible for severe losses in winter and spring pea crops. Despite different climatic conditions, epidemics on winter and spring crops are due to a single population ofD. pinodes, suggesting gene flow either between the two crops or from reservoir sources during the cropping season. This should lead to similar pathogenicity characteristics in isolates sampled from the two crops. However, these hypotheses have never been formally tested. We therefore sampled a total of 520D. pinodesstrains throughout a growing season from winter and spring pea plots (WP and SP, respectively) and from winter and spring trap plants (TWP and TSP). Amplified fragment length polymorphism (AFLP) markers revealed high genetic diversity within subpopulations, whereas pathogenicity tests showed that mean aggressiveness increases over the course of an epidemic. These results support the idea that alloinoculum contributes to the carryover of epidemics between winter and spring crops and that the most aggressive isolates are selected as an epidemic progresses.IMPORTANCEAscochyta blight, caused byDidymella pinodes, is responsible for severe losses in pea crops. While previous studies have shown that ascochyta blight epidemics on winter and spring crops are due to a single population ofD. pinodes, suggesting that isolates from the two crops present similar pathogenicity characteristics, that hypothesis have never been tested. Genetic analysis of subpopulations sampled throughout a growing season from winter and spring pea plots revealed high genetic diversity within subpopulations, whereas pathogenicity tests showed that mean aggressiveness increases over the course of an epidemic.

scholarly journals Comparative Genetic Diversity of Pseudomonas stutzeri Genomovars, Clonal Structure, and Phylogeny of the Species

2004 ◽  
Vol 186 (16) ◽  
pp. 5239-5248 ◽  
Author(s):  
Aina Maria Cladera ◽  
Antonio Bennasar ◽  
Maria Barceló ◽  
Jorge Lalucat ◽  
Elena García-Valdés
Keyword(s):  
Genetic Diversity ◽  
Sequence Analysis ◽  
Pseudomonas Stutzeri ◽  
Clonal Structure ◽  
High Genetic Diversity ◽  
Closely Related Species ◽  
16S Ribosomal Dna ◽  
Internally Transcribed Spacer ◽  
Type Strains ◽  
Good Agreement

ABSTRACT A combined phylogenetic and multilocus DNA sequence analysis of 26 Pseudomonas stutzeri strains distributed within the 9 genomovars of the species has been performed. Type strains of the two most closely related species (P. balearica, former genomovar 6, and P. mendocina), together with P. aeruginosa, as the type species of the genus, have been included in the study. The extremely high genetic diversity and the clonal structure of the species were confirmed by the sequence analysis. Clustering of strains in the consensus phylogeny inferred from the analysis of seven nucleotide sequences (16S ribosomal DNA, internally transcribed spacer region 1, gyrB, rpoD, nosZ, catA, and nahH) confirmed the monophyletic origin of the genomovars within the Pseudomonas branch and is in good agreement with earlier DNA-DNA similarity analysis, indicating that the selected genes are representative of the whole genome in members of the species.

scholarly journals Brown trout (Salmo trutta L.) high genetic diversity around the Tyrrhenian Sea as revealed by nuclear and mitochondrial markers

Hydrobiologia ◽  
2018 ◽  
Vol 826 (1) ◽  
pp. 209-231 ◽  
Author(s):  
P. Berrebi ◽  
V. Caputo Barucchi ◽  
A. Splendiani ◽  
S. Muracciole ◽  
A. Sabatini ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Tyrrhenian Sea ◽  
High Genetic Diversity ◽  
Mitochondrial Markers ◽  
Brown Trout Salmo Trutta

scholarly journals Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1894 ◽  
Author(s):  
Nikolett Sziszkosz ◽  
Sándor Mihók ◽  
András Jávor ◽  
Szilvia Kusza
Keyword(s):  
Genetic Diversity ◽  
Genetic Characterization ◽  
Horse Breed ◽  
High Genetic Diversity ◽  
Mitochondrial Markers ◽  
Blast Analysis ◽  
D Loop ◽  
First Time ◽  
Mtdna Markers

The Gidran is a native Hungarian horse breed that has approached extinction several times. Phylogenetic analysis of two mitochondrial markers (D-loop and cytochrome-b) was performed to determine the genetic characterization of the Gidran for the first time as well as to detect errors in the management of the Gidran stud book. Sequencing of 686 bp ofCYTBand 202 bp of the D-loop in 260 mares revealed 24 and 32 haplotypes, respectively, among 31 mare families. BLAST analysis revealed six novelCYTBand four D-loop haplotypes that have not been previously reported. The Gidran mares showed high haplotype (CYTB: 0.8735 ± 0.011; D-loop: 0.9136 ± 0.008) and moderate nucleotide (CYTB: 0.00472 ± 0.00017; D-loop: 0.02091 ± 0.00068) diversity. Of the 31 Gidran mare families, only 15CYTB(48.4%) and 17 D-loop (54.8%) distinct haplotypes were formed using the two markers separately. Merged markers created 24 (77.4%) mare families, which were in agreement with the mare families in the stud book. Our key finding was that the Gidran breed still possesses high genetic diversity despite its history. The obtained haplotypes are mostly consistent with known mare families, particularly when the two mtDNA markers were merged. Our results could facilitate conservation efforts for preserving the genetic diversity of the Gidran.

scholarly journals Rhipicephalus sanguineus Complex in the Americas: Systematic, Genetic Diversity, and Geographic Insights

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1118
Author(s):  
Sokani Sánchez-Montes ◽  
Beatriz Salceda-Sánchez ◽  
Sergio E. Bermúdez ◽  
Gabriela Aguilar-Tipacamú ◽  
Gerardo G. Ballados-González ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rhipicephalus Sanguineus ◽  
Northern Limit ◽  
High Genetic Diversity ◽  
Mitochondrial Markers ◽  
Genetic Diversity And Structure ◽  
Morphological Variants ◽  
Wide Geographic Distribution ◽  
Rhipicephalus Sanguineus Group ◽  
Vectorial Competence

The Rhipicephalus sanguineus group encompasses at least 12 validated species of Palearctic and Afrotropical hard ticks, which are relevant in veterinary medicine and public health. The taxonomy of R. sanguineus s.s., has been particularly intensely debated, due to its wide geographic distribution, morphological variants, parasite-host associations, and its capacity and vectorial competence for the transmission of several pathogens. By sequencing mitochondrial markers, it was possible to identify the existence of multiple lineages, among which the Tropical and the Temperate lineages stand out, particularly in America. However, the northern limit between these lineages is not clear due to the lack of extensive sampling across Mexico. For this reason, the aim of the present study was to determine the genetic diversity and structure of the R. sanguineus group in Mexico and to compare it with the populations reported in the Americas, in order to propose the northern limit of the R. sanguineus Tropical lineage and the potential regions of sympatry with R. sanguineus s.s. The findings of this study now confirm the presence of R. sanguineus s.s. in Mexico, showing a subtle genetic structure and high genetic diversity throughout its distribution in the Americas. In contrast, the Tropical lineage seems to be genetically less diverse in its overall distribution in the Americas. The genetic diversity of these two independent lineages could have important epidemiological implications in the transmission of tick pathogens.

scholarly journals Diversity of Diazotrophic Unicellular Cyanobacteria in the Tropical North Atlantic Ocean

2002 ◽  
Vol 68 (11) ◽  
pp. 5760-5764 ◽  
Author(s):  
Luisa I. Falcón ◽  
Frank Cipriano ◽  
Andrei Y. Chistoserdov ◽  
Edward J. Carpenter
Keyword(s):  
Genetic Diversity ◽  
Atlantic Ocean ◽  
Ribosomal Dna ◽  
North Atlantic ◽  
Dna Sequences ◽  
North Pacific ◽  
North Atlantic Ocean ◽  
16S Ribosomal Dna ◽  
Tropical North Atlantic ◽  
Phylogenetic Affinities

ABSTRACT We present data on the genetic diversity and phylogenetic affinities of N2-fixing unicellular cyanobacteria in the plankton of the tropical North Atlantic Ocean. Our dinitrogenase gene (nifH) sequences grouped together with a group of cyanobacteria from the subtropical North Pacific; another subtropical North Pacific group was only distantly related. Most of the 16S ribosomal DNA sequences from our tropical North Atlantic samples were closely allied with sequences from a symbiont of the diatom Climacodium frauenfeldianum. These findings suggest a complex pattern of evolutionary and ecological divergence among unicellular cyanobacteria within and between ocean basins.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

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