Cytotoxicity and genotoxicity of blue LED light and protective effects of AA2G in mammalian cells and associated DNA repair deficient cell lines

Xeroderma Pigmentosum (XP) is a DNA repair disease characterized by nucleotide excision repair (NER) malfunction, leading to photosensitivity and increased incidence of skin malignancies. The role of XP-A in NER pathways has been well studied while discrepancies associated with ROS levels and the role of radical species between normal and deficient XPA cell lines have been observed. Using liquid chromatography tandem mass spectrometry we have determined the four 5’,8-cyclopurines (cPu) lesions (i.e., 5′R-cdG, 5′S-cdG, 5′R-cdA and 5′S-cdA), 8-oxo-dA and 8-oxo-dG in wt (EUE-pBD650) and XPA-deficient (EUE-siXPA) human embryonic epithelial cell lines, under different oxygen tension (hyperoxic 21%, physioxic 5% and hypoxic 1%). The levels of Fe and Cu were also measured. The main findings of our study were: (i) the total amount of cPu (1.82–2.52 lesions/106 nucleotides) is the same order of magnitude as 8-oxo-Pu (3.10–4.11 lesions/106 nucleotides) in both cell types, (ii) the four cPu levels are similar in hyperoxic and physioxic conditions for both wt and deficient cell lines, whereas 8-oxo-Pu increases in all cases, (iii) both wt and deficient cell lines accumulated high levels of cPu under hypoxic compared to physioxic conditions, whereas the 8-oxo-Pu levels show an opposite trend, (iv) the diastereoisomeric ratios 5′R/5′S are independent of oxygen concentration being 0.29 for cdG and 2.69 for cdA for EUE-pBD650 (wt) and 0.32 for cdG and 2.94 for cdA for EUE-siXPA (deficient), (v) in deficient cell lines Fe levels were significantly higher. The data show for the first time the connection of oxygen concentration in cells with different DNA repair ability and the levels of different DNA lesions highlighting the significance of cPu. Membrane lipidomic data at 21% O2 indicated differences in the fatty acid contents between wild type and deficient cells, envisaging functional effects on membranes associated with the different repair capabilities, to be further investigated.

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Effect of gamma rays on efficiency of gene transfer in DNA repair-proficient and -deficient cell lines

Somatic Cell and Molecular Genetics ◽

10.1007/bf01535315 ◽

1988 ◽

Vol 14 (6) ◽

pp. 613-621 ◽

Cited By ~ 6

Author(s):

Jaime S. Rubin

Keyword(s):

Dna Repair ◽

Gene Transfer ◽

Cell Lines ◽

Gamma Rays ◽

Deficient Cell

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Comparison of cellular lethality in DNA repair-proficient or -deficient cell lines resulting from exposure to 70 MeV/n protons or 290 MeV/n carbon ions

Oncology Reports ◽

10.3892/or.2012.1982 ◽

2012 ◽

Vol 28 (5) ◽

pp. 1591-1596 ◽

Cited By ~ 8

Author(s):

STEFAN C. GENET ◽

JUNKO MAEDA ◽

HIROSHI FUJISAWA ◽

CHARLES R. YURKON ◽

YOSHIHIRO FUJII ◽

...

Keyword(s):

Dna Repair ◽

Cell Lines ◽

Carbon Ions ◽

Deficient Cell

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Experience in application of red and blue LED light with infra+red component in the ENT disease treatment in children

SOVREMENNAYA PEDIATRIYA ◽

10.15574/sp.2018.89.73 ◽

2018 ◽

pp. 73-79

Author(s):

A.L. Kosakovskyi ◽

◽

S.O. Gulyar ◽

I.A. Kosakivska ◽

N.P. Grushetska ◽

...

Keyword(s):

Disease Treatment ◽

Blue Led ◽

Led Light ◽

Infra Red

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Photocontrolled iodine-mediated reversible-deactivation radical polymerization with a semifluorinated alternating copolymer as the macroinitiator

Polymer Chemistry ◽

10.1039/d0py01357a ◽

2020 ◽

Vol 11 (47) ◽

pp. 7497-7505

Author(s):

Jiannan Cheng ◽

Kai Tu ◽

Enjie He ◽

Jinying Wang ◽

Lifen Zhang ◽

...

Keyword(s):

Block Copolymers ◽

Radical Polymerization ◽

Room Temperature ◽

Blue Led ◽

Led Light ◽

Alternating Copolymers ◽

Alternating Copolymer ◽

Reversible Deactivation ◽

Novel Strategy ◽

Reversible Deactivation Radical Polymerization

A novel strategy for preparing block copolymers with semifluorinated alternating copolymers as macroinitiators was established by photocontrolled iodine-mediated RDRP under irradiation with blue LED light at room temperature.

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Exploiting DNA Endonucleases to Advance Mechanisms of DNA Repair

Biology ◽

10.3390/biology10060530 ◽

2021 ◽

Vol 10 (6) ◽

pp. 530

Author(s):

Marlo K. Thompson ◽

Robert W. Sobol ◽

Aishwarya Prakash

Keyword(s):

Dna Repair ◽

Mammalian Cells ◽

Genome Stability ◽

Gene Editing ◽

Adaptive Immune System ◽

Zinc Finger Nucleases ◽

Specific Gene ◽

Target Sequence ◽

Transcription Activator ◽

Low Cytotoxicity

The earliest methods of genome editing, such as zinc-finger nucleases (ZFN) and transcription activator-like effector nucleases (TALENs), utilize customizable DNA-binding motifs to target the genome at specific loci. While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems. The discovery of clustered regularly interspaced short palindromic repeat sequences (CRISPR) in Escherichia coli dates to 1987, yet it was another 20 years before CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection. By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells. The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many. In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.

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Microbicidal effect of 405-nm blue LED light on Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin

Lasers in Medical Science ◽

10.1007/s10103-021-03323-z ◽

2021 ◽

Author(s):

Chiaki Tsutsumi-Arai ◽

Yuki Arai ◽

Chika Terada-Ito ◽

Takahiro Imamura ◽

Seiko Tatehara ◽

...

Keyword(s):

Candida Albicans ◽

Streptococcus Mutans ◽

Denture Base ◽

Blue Led ◽

Led Light ◽

Base Resin ◽

Denture Base Resin

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Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies

Applied Sciences ◽

10.3390/app11062534 ◽

2021 ◽

Vol 11 (6) ◽

pp. 2534

Author(s):

Henrike Rebl ◽

Claudia Bergemann ◽

Sebastian Rakers ◽

Barbara Nebe ◽

Alexander Rebl

Keyword(s):

Plasma Treatment ◽

Cell Lines ◽

Mammalian Cells ◽

Atmospheric Pressure Plasma ◽

Fish Farming ◽

Skin Lesions ◽

Atmospheric Plasma ◽

Fish Cell ◽

Farmed Fish ◽

Fish Cells

The present study provides the fundamental results for the treatment of marine organisms with cold atmospheric pressure plasma. In farmed fish, skin lesions may occur as a result of intensive fish farming. Cold atmospheric plasma offers promising medical potential in wound healing processes. Since the underlying plasma-mediated mechanisms at the physical and cellular level are yet to be fully understood, we investigated the sensitivity of three fish cell lines to plasma treatment in comparison with mammalian cells. We varied (I) cell density, (II) culture medium, and (III) pyruvate concentration in the medium as experimental parameters. Depending on the experimental setup, the plasma treatment affected the viability of the different cell lines to varying degrees. We conclude that it is mandatory to use similar cell densities and an identical medium, or at least a medium with identical antioxidant capacity, when studying plasma effects on different cell lines. Altogether, fish cells showed a higher sensitivity towards plasma treatment than mammalian cells in most of our setups. These results should increase the understanding of the future treatment of fish.

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