The effects of surface functionality and size of gold nanoparticles on neuronal toxicity, apoptosis, ROS production and cellular/suborgan biodistribution

(1) Background: Chitosan-coated gold nanoparticles (CH-AuNPs) have important theranostic applications in biomedical sciences, including cancer research. However, although cell cytotoxicity has been studied in cancerous cells, little is known about their effect in proliferating primary leukocytes. Here, we assessed the effect of CH-AuNPs and the implication of ROS on non-cancerous endothelial and fibroblast cell lines and in proliferative lymphoid cells. (2) Methods: The Turkevich method was used to synthetize gold nanoparticles. We tested cell viability, cell death, ROS production, and cell cycle in primary lymphoid cells, compared with non-cancer and cancer cell lines. Concanavalin A (ConA) or lipopolysaccharide (LPS) were used to induce proliferation on lymphoid cells. (3) Results: CH-AuNPs presented high cytotoxicity and ROS production against cancer cells compared to non-cancer cells; they also induced a different pattern of ROS production in peripheral blood mononuclear cells (PBMCs). No significant cell-death difference was found in PBMCs, splenic mononuclear cells, and bone marrow cells (BMC) with or without a proliferative stimuli. (4) Conclusions: Taken together, our results highlight the selectivity of CH-AuNPs to cancer cells, discarding a consistent cytotoxicity upon proliferative cells including endothelial, fibroblast, and lymphoid cells, and suggest their application in cancer treatment without affecting immune cells.

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The Interplay of Size and Surface Functionality on the Cellular Uptake of Sub-10 nm Gold Nanoparticles

ACS Nano ◽

10.1021/acsnano.5b03521 ◽

2015 ◽

Vol 9 (10) ◽

pp. 9986-9993 ◽

Cited By ~ 185

Author(s):

Ying Jiang ◽

Shuaidong Huo ◽

Tsukasa Mizuhara ◽

Riddha Das ◽

Yi-Wei Lee ◽

...

Keyword(s):

Gold Nanoparticles ◽

Cellular Uptake ◽

Surface Functionality

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Do particle size and surface functionality affect uptake and depuration of gold nanoparticles by aquatic invertebrates?

Environmental Toxicology and Chemistry ◽

10.1002/etc.2868 ◽

2015 ◽

Vol 34 (4) ◽

pp. 850-859 ◽

Cited By ~ 12

Author(s):

Sujung Park ◽

James Woodhall ◽

Guibin Ma ◽

Jonathan G.C Veinot ◽

Alistair B.A. Boxall

Keyword(s):

Particle Size ◽

Gold Nanoparticles ◽

Aquatic Invertebrates ◽

Surface Functionality

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Facile synthesis of gold nanoparticles on propylamine functionalized SBA-15 and effect of surface functionality of its enhanced bactericidal activity against gram positive bacteria

Materials Research Express ◽

10.1088/2053-1591/2/7/075402 ◽

2015 ◽

Vol 2 (7) ◽

pp. 075402 ◽

Cited By ~ 5

Author(s):

Diganta Bhuyan ◽

Animesh Gogoi ◽

Mrinal Saikia ◽

Ratul Saikia ◽

Lakshi Saikia

Keyword(s):

Gold Nanoparticles ◽

Bactericidal Activity ◽

Facile Synthesis ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Surface Functionality ◽

Effect Of Surface

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Gold Particles Recovery using Novel Reducing Agent with Regard to Continuous Approach

Current Nanoscience ◽

10.2174/1573413717666210322114205 ◽

2021 ◽

Vol 17 ◽

Author(s):

Alireza Fotovat ◽

Alireza Feizbakhsh ◽

Ali Bagheri Garmaroudi

Keyword(s):

Particle Size ◽

Gold Nanoparticles ◽

Synthesis Method ◽

Volume Ratio ◽

X Ray Diffraction ◽

Gold Particles ◽

Surface Functionality ◽

Gold Particle Size ◽

Recycling Potential ◽

Biological Methods

Background: Over 300 tonnes of gold utilised in electronics devices every year, end-of-life electronic equipment offers an crucial recycling potential for secondary gold supply. Electronic boards are among common and exploitable sources of gold that are widely used in various industry. The aims of this paper is to use gold element of electronic boards by adjusting its surface functionality and particle size to make this metal to be used in various applications Methods: to gain this purpose by using acidic solutions and up to bottom chemical synthesis method among physical, chemical, and biological methods and by transferring gold particle size from micrometer to nanometer, surface-area-to-volume ratio will be increase such that new structural properties emerge, change, or improve. Results: Tests such as Fourier-Transform Infrared spectroscopy, X-Ray Diffraction, and Field Emission Scanning Electron Microscopy have confirmed high-quality production and proper extraction of gold particles on a nanometer scale from electronic plates and confirmed crystalline structure of extracted gold in range of 70-110 nm. Conclusion: The main objective of this research is to derive and fabricate gold nanoparticles using chemical oxidative route using HCl and Aqua regia to produce gold nanoparticles in Nano dimension scale. Results has shown that gold nanoparticles are synthesized successfully via chemical process. Moreover, Acid concentration and reaction time has enormous effect on production procedure.

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Mimosine functionalized gold nanoparticles (Mimo-AuNPs) suppress β-amyloid aggregation and neuronal toxicity

Bioactive Materials ◽

10.1016/j.bioactmat.2021.04.029 ◽

2021 ◽

Vol 6 (12) ◽

pp. 4491-4505

Author(s):

Bibin G. Anand ◽

Qi Wu ◽

Govindarajan Karthivashan ◽

Kiran P. Shejale ◽

Sara Amidian ◽

...

Keyword(s):

Gold Nanoparticles ◽

Amyloid Aggregation ◽

Functionalized Gold Nanoparticles ◽

Neuronal Toxicity ◽

Β Amyloid

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Effect of Size, Shape, and Surface Modification on Cytotoxicity of Gold Nanoparticles to Human HEp-2 and Canine MDCK Cells

Journal of Nanomaterials ◽

10.1155/2012/375496 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 38

Author(s):

Yinan Zhang ◽

Dan Xu ◽

Wenqin Li ◽

Jun Yu ◽

Yu Chen

Keyword(s):

Gold Nanoparticles ◽

Cell Death ◽

Surface Charge ◽

Mdck Cells ◽

Time Lapse ◽

Biological Research ◽

Dependent Manner ◽

Surface Functionality ◽

Aspect Ratios ◽

Dose Dependent

There have been increasing interests in applying gold nanoparticles in biological research, drug delivery, and therapy. As the interaction of gold nanoparticles with cells relies on properties of nanoparticles, the cytotoxicity is complex and still under debating. In this work, we investigate the cytotoxicity of gold nanoparticles of different encapsulations, surface charge states, sizes and shapes to both human HEp-2 and canine MDCK cells. We found that cetyltrimethylammonium-bromide- (CTAB-) encapsulated gold nanorods (GNRs) were relatively higher cytotoxic than GNRs undergone further polymer coating and citrate stabilized gold nanospheres (GNSs). The toxicity of CTAB-encapsulated GNRs was mainly caused by CTAB on GNRs' surface but not free CTAB in the solution. No obvious difference was found among GNRs of different aspect ratios. Time-lapse study revealed that cell death caused by GNRs occurred predominately within one hour through apoptosis, whereas cell death by free CTAB was in a time- and dose-dependent manner. Both positively and negatively surface-charged polymer-coated GNRs (PSS-GNRs and PAH-PSS-GNRs) showed similar levels of cytotoxic, suggesting the significance of surface functionality rather than surface charge in this case.

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Co-initiated hyperbranched-polydendron building blocks for the direct nanoprecipitation of dendron-directed patchy particles with heterogeneous surface functionality

Polymer Chemistry ◽

10.1039/c8py00291f ◽

2018 ◽

Vol 9 (14) ◽

pp. 1767-1771 ◽

Cited By ~ 6

Author(s):

F. Y. Hern ◽

A. Hill ◽

A. Owen ◽

S. P. Rannard

Keyword(s):

Gold Nanoparticles ◽

Building Blocks ◽

Heterogeneous Surface ◽

Branched Polymer ◽

Surface Functionality ◽

Synthetic Strategy ◽

Patchy Particles ◽

Rapid Construction ◽

Patchy Nanoparticles

A synthetic strategy branched polymer building blocks that allow the rapid construction of patchy nanoparticles is presented. Hyperbranched polydendrons with mixtures of PEG and thiol-functional dendrons nanoprecipitate to form isolated zones that are imaged with gold nanoparticles.

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