Biochemical methane potential trial of terrestrial weeds: Evolution of mono digestion and co-digestion on biogas production

The present study evaluates the digestion of cork boiling wastewater (CBW) through a biochemical methane potential (BMP) test. BMP assays were carried out with a working volume of 600 mL at a constant mesophilic temperature (35 °C). The experiment bottles contained CBW and inoculum (digested sludge from a wastewater treatment plant (WWTP)), with a ratio of inoculum/substrate (Ino/CBW) of 1:1 and 2:1 on the basis of volatile solids (VSs); the codigestion with food waste (FW) had a ratio of 2/0.7:0.3 (Ino/CBW:FW) and the codigestion with cow manure (CM) had a ratio of 2/0.5:0.5 (Ino/CBW:CM). Biogas and methane production was proportional to the inoculum substrate ratio (ISR) used. BMP tests have proved to be valuable for inferring the adequacy of anaerobic digestion to treat wastewater from the cork industry. The results indicate that the biomethane potential of CBWs for Ino/CBW ratios 1:1 and 2:1 is very low compared to other organic substrates. For the codigestion tests, the test with the Ino/CBW:CM ratio of 2/0.7:0.3 showed better biomethane yields, being in the expected values. This demonstrated that it is possible to perform the anaerobic digestion (AD) of CBW using a cosubstrate to increase biogas production and biomethane and to improve the quality of the final digestate.

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Evaluation of Feasibility of Using the Bacteriophage T4 Lysozyme to Improve the Hydrolysis and Biochemical Methane Potential of Secondary Sludge

Energies ◽

10.3390/en12193644 ◽

2019 ◽

Vol 12 (19) ◽

pp. 3644

Author(s):

Sangmin Kim ◽

Seung-Gyun Woo ◽

Joonyeob Lee ◽

Dae-Hee Lee ◽

Seokhwan Hwang

Keyword(s):

Cell Wall ◽

Bacterial Cell ◽

Wastewater Treatment Plants ◽

Biogas Production ◽

Bacteriophage T4 ◽

Suspended Solid ◽

T4 Lysozyme ◽

Biochemical Methane Potential ◽

Secondary Sludge ◽

Methane Potential

Anaerobic digestion (AD) of secondary sludge is a rate-limiting step due to the bacterial cell wall. In this study, experiments were performed to characterize secondary sludges from three wastewater treatment plants (WWTPs), and to investigate the feasibility of using bacteriophage lysozymes to speed up AD by accelerating the degradation of bacterial cell walls. Protein was the main organic material (67.7% of volatile solids in the sludge). The bacteriophage T4 lysozyme (T4L) was tested for hydrolysis and biochemical methane potential. Variations in the volatile suspended solid (VSS) concentration and biogas production were monitored. The VSS reduction efficiencies by hydrolysis using T4L for 72 h increased and ranged from 17.8% to 26.4%. Biogas production using T4L treated sludges increased and biogas production was increased by as much as 82.4%. Biogas production rate also increased, and the average reaction rate coefficient of first-order kinetics was 0.56 ± 0.02/d, which was up to 47.5% higher compared to the untreated samples at the maximum. Alphaproteobacteria, Betaproteobacteria, Flavobacteriia, Gammaproteobacteria, and Sphingobacteriia were major microbial classes in all sludges. The interpretation of the microbial community structure indicated that T4L treatment is likely to increase the rate of cell wall digestion.

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Biogas Production from Physicochemically Pretreated Grass Lawn Waste: Comparison of Different Process Schemes

Molecules ◽

10.3390/molecules25020296 ◽

2020 ◽

Vol 25 (2) ◽

pp. 296 ◽

Cited By ~ 2

Author(s):

Georgia Antonopoulou ◽

Dimitrios Vayenas ◽

Gerasimos Lyberatos

Keyword(s):

Biogas Production ◽

Economic Assessment ◽

Methane Yield ◽

Biochemical Methane Potential ◽

Naoh Pretreatment ◽

Volatile Solids ◽

Methane Potential ◽

Whole Slurry ◽

Almost All ◽

Pretreated Biomass

Various pretreatment methods, such as thermal, alkaline and acid, were applied on grass lawn (GL) waste and the effect of each pretreatment method on the Biochemical Methane Potential was evaluated for two options, namely using the whole slurry resulting from pretreatment or the separate solid and liquid fractions obtained. In addition, the effect of each pretreatment on carbohydrate solubilization and lignocellulossic content fractionation (to cellulose, hemicellulose, lignin) was also evaluated. The experimental results showed that the methane yield was enhanced with alkaline pretreatment and, the higher the NaOH concentration (20 g/100 gTotal Solids (TS)), the higher was the methane yield observed (427.07 L CH4/kg Volatile Solids (VS), which was almost 25.7% higher than the BMP of the untreated GL). Comparing the BMP obtained under the two options, i.e., that of the whole pretreatment slurry with the sum of the BMPs of both fractions, it was found that direct anaerobic digestion without separation of the pretreated biomass was favored, in almost all cases. A preliminary energy balance and economic assessment indicated that the process could be sustainable, leading to a positive net heat energy only when using a more concentrated pretreated slurry (i.e., 20% organic loading), or when applying NaOH pretreatment at a lower chemical loading.

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Influence of lignin on biochemical methane potential of biomass for biogas production

Current Opinion in Biotechnology ◽

10.1016/j.copbio.2011.05.484 ◽

2011 ◽

Vol 22 ◽

pp. S146

Author(s):

Jin M Triolo ◽

Sven G Sommer ◽

Henrik B Moller ◽

Martin R Weisbjerg ◽

Xinyuan Jiang

Keyword(s):

Biogas Production ◽

Biochemical Methane Potential ◽

Methane Potential

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Optimisation of C:N Ratio for Co-Digested Processed Industrial Food Waste and Sewage Sludge Using the BMP Test

International Journal of Chemical Reactor Engineering ◽

10.1515/1542-6580.2327 ◽

2011 ◽

Vol 9 (1) ◽

Cited By ~ 5

Author(s):

Zuhaib Siddiqui ◽

N.J. Horan ◽

Kofi Anaman

Keyword(s):

Sewage Sludge ◽

Food Waste ◽

Biogas Production ◽

C:N Ratio ◽

Methane Yield ◽

Biochemical Methane Potential ◽

Volatile Solids ◽

Methane Potential ◽

Biomethane Production ◽

Bmp Test

Biomethane production from processed industrial food waste (IFW) in admixture with sewage sludge (primary and waste activated sludge: PS and WAS) was evaluated at a range of C:N ratios using a standard biochemical methane potential (BMP) test. IFW alone had a C:N of 30 whereas for WAS it was 5.4 and thus the C:N ratio of the blends fell in that range. Increasing the IFW content in mix improves the methane potential by increasing both the cumulative biogas production and the rate of methane production. Optimum methane yield 239 mL/g VSremoved occurred at a C:N ratio of 15 which was achieved with a blend containing 11 percent (w/w) IFW. As the fraction of IFW in the blend increased, volatile solids (VS) destruction was increased and this led to a reduction in methane yield and amount of production. The highest destruction of volatile solids of 93 percent was achieved at C:N of 20 followed by C:N 30 and 15. A shortened BMP test is adequate for evaluating optimum admixtures.

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Chemical pre-treatments on oil palm empty fruit bunches: Impacts on characteristics and methane potential

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/924/1/012071 ◽

2021 ◽

Vol 924 (1) ◽

pp. 012071

Author(s):

N A Rohma ◽

S Suhartini ◽

I Nurika

Keyword(s):

Lignocellulosic Biomass ◽

Oil Palm ◽

Biogas Production ◽

Lignin Content ◽

Test System ◽

Biochemical Methane Potential ◽

Empty Fruit Bunches ◽

Methane Potential ◽

Pre Treatment ◽

Bmp Test

Abstract Production of biogas from lignocellulosic biomass by anaerobic digestion (AD) has attracted much interest. Oil palm empty fruit bunches (OPEFB), one of lignocellulosic biomass, is highly abundant in Indonesia and has potential as feedstock for bioenergy production such as biogas or methane. Yet, pre-treatments are needed to improve biogas production due to its complex crystalline structures. Chemical pre-treatments with acid or alkaline solution were reported to increase cellulose or highly reduce the lignin content of OPEFB. This study aimed to evaluate the effect of acid and alkaline pre-treatments on the characteristics of OPEFB and methane potential. The acid pre-treatment experimental design was used factor of H2SO4 concentration (1, 1.3, and 1.6 (%v/v)) and NaOH concentration (1.8, 2.8, and 3.8 (%w/v)). Methane potential evaluation was carried out using the biochemical methane potential (BMP) test with the Automatic Methane Potential Test System (AMPTS) II under mesophilic condition (37°C), operated for 28 days. The results showed that both dilute acid and alkaline pre-treatment positively impact altering the characteristics of OPEFB, hence the specific methane potential. Alkaline pre-treatment with NaOH 3.8 (%w/v) gave the highest average SMP value of 0.161 ± 0.005 m3 CH4/kgVSadded.

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Mesophilic Fermentation of SOMW in a Micro Pilot-Scale Anaerobic Digester

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.827.84 ◽

2013 ◽

Vol 827 ◽

pp. 84-90 ◽

Cited By ~ 3

Author(s):

Maurizio Carlini ◽

Sonia Castellucci ◽

Silvia Cocchi

Keyword(s):

Anaerobic Digestion ◽

Energy Production ◽

Biogas Production ◽

Ph Control ◽

Pilot Scale ◽

Biochemical Methane Potential ◽

Mediterranean Countries ◽

Methane Potential ◽

Environmentally Sound ◽

Bmp Test

One of the most promising processes to exploit Solid Olive-Mill Waste (SOMW) for energy production is anaerobic digestion. An experimental study has been carried out on SOMW and inoculum, consisting of Cattle Slurry Digested (CSD) and coming from an anaerobic digestion plant. A substrate with an optimal supply ratio equal to 2:1 has been investigated in a reactor at 37°C by analysing the biogas production. The Biochemical Methane Potential (BMP) test has been carried out, monitoring pH, biogas production (amount and composition). According to the tests results, SOMWs needed to be diluted and inoculated, moreover the pH control is foundamental in order to obtain a significant biogas production. Anaerobic digestion plant of SOMW should be promoted in Mediterranean countries as an environmentally sound option for waste management and energy production, since olive mills are very widespread agro-industries in this area.

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Kinetics and Nanoparticle Catalytic Enhancement of Biogas Production from Wastewater Using a Magnetized Biochemical Methane Potential (MBMP) System

Catalysts ◽

10.3390/catal10101200 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1200

Author(s):

Emmanuel Kweinor Tetteh ◽

Sudesh Rathilal

Keyword(s):

Biogas Production ◽

Organic Fertilizer ◽

Gompertz Model ◽

Lag Phase ◽

Biochemical Methane Potential ◽

Test Technique ◽

X Ray ◽

Jar Test ◽

Slow Kinetics ◽

Methane Potential

This study presents magnetized nanoparticles (NPs) as a catalyst to accelerate anaerobic digestion (AD) potential for clean and ecofriendly energy (biogas) from wastewater settings. The effects of iron oxides (Ms) and aluminum sulphate (Alum) were investigated using two chronological experiments: (i) the Jar test technique to generate residue slurry as organic fertilizer potential and (ii) a magnetized biochemical methane potential (MBMP) system for biogas production at mesophilic conditions for 21 days. X-ray diffraction and Fourier Transform Infrared spectroscopy were carried out to establish the Ms Crystallite and active functional groups respectively. Scanning electronic microscopy coupled with energy dispersive X-ray spectrometer and elemental analysis were used to track and confirm NPs inclusion after the post-AD process. Coagulation at 50 mg/L and magnetic exposure time of 30 min showed above 85% treatability performance by Ms as compared to 70% for Alum. Owing to the slow kinetics of the AD process, additional NPs content in the digesters coupled with an external magnetic field improved their performance. Cumulative biogas yields of 1460 mL/d > 610 mL/d > 505 mL/d for Ms > Control > Alum respectively representing 80% > 61% > 52% of CH4 were attained. The modified Gompertz model shows that the presence of NPs shortens the lag phase of the control system with kinetics rate constants of 0.285 1/d (control) to 0.127 1/d (Ms) < 0.195 1/d (Alum).

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