Inhibitory effect of Lactobacillus plantarum and Lb. fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections

In this study Candida speices was diagnosed in 26 swab samples from patients with denture stomatitis , investigates the antagonism activity of Lactobacillus was investigated against the yeast of Candida albicans in vitro.Results revealed that The inhibition effect of Lactic Acid Bacteria against C.albicans was examined in solid medium, L.plantarum gave higher inhibition average 11mm followed by L.acidophillus with average 9 mm and, L.fermentum , L.casei with averages 7 mm. Whereas the filtrates, the highest inhibition zone were 20 and 16 mm by L. plantarum and L.acidophillus, respectively.

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Preventive Effect of Lactobacillus Plantarum CQPC10 on Activated Carbon Induced Constipation in Institute of Cancer Research (ICR) Mice

Applied Sciences ◽

10.3390/app8091498 ◽

2018 ◽

Vol 8 (9) ◽

pp. 1498 ◽

Cited By ~ 14

Author(s):

Jing Zhang ◽

Xianrong Zhou ◽

Benshou Chen ◽

Xingyao Long ◽

Jianfei Mu ◽

...

Keyword(s):

Nitric Oxide ◽

Activated Carbon ◽

Nitric Oxide Synthase ◽

Lactobacillus Plantarum ◽

Quantitative Polymerase Chain Reaction ◽

Inhibitory Effect ◽

Lactobacillus Bulgaricus ◽

Control Group ◽

Expression Levels ◽

Oxide Synthase

Chinese Paocai is a traditional fermented food containing an abundance of beneficial microorganisms. In this study, the microorganisms in Szechwan Paocai were isolated and identified, and a strain of lactic acid bacteria (Lactobacillus plantarum CQPC10, LP-CQPC10) was found to exert an inhibitory effect on constipation. Microorganisms were isolated and identified via 16S rDNA. Activated carbon was used to induce constipation in a mouse model and the inhibitory effect of LP-CQPC10 on this induced constipation was investigated via both pathological sections and qPCR (quantitative polymerase chain reaction). A strain of Lactobacillus plantarum was identified and named LP-CQPC10. The obtained results showed that, as compared to the control group, LP-CQPC10 significantly inhibited the amount, weight, and water content of faeces. The defecation time of the first tarry stool was significantly shorter in LP-CQPC10 groups than in the control group. The activated carbon progradation rate was significantly higher when compared to the control group and the effectiveness was improved. LP-CQPC10 increased the serum levels of MTL (motilin), Gas (gastrin), ET (endothelin), AchE (acetylcholinesterase), SP (substance P), and VIP (vasoactive intestinal peptide), while decreasing the SS (somatostatin) level. Furthermore, it improved the GSH (glutathione) level and decreased the MPO (myeloperoxidase), MDA (malondialdehyde), and NO (nitric oxide) levels. The results of qPCR indicated that LP-CQPC10 significantly up-regulated the mRNA expression levels of c-Kit, SCF (stem cell factor), GDNF (glial cell-derived neurotrophic factor), eNOS (endothelial nitric oxide synthase), nNOS (neuronal nitric oxide synthase), and AQP3 (aquaporin-3), while down-regulating the expression levels of TRPV1 (transient receptor potential cation channel subfamily V member 1), iNOS (inducible nitric oxide synthase), and AQP9 (aquaporin-9). LP-CQPC10 showed a good inhibitory effect on experimentally induced constipation, and the obtained effectiveness is superior to that of Lactobacillus bulgaricus, indicating the better probiotic potential of this strain.

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Inhibitory effect of sweet whey fermented by Lactobacillus plantarum strains against fungal growth: A potential application as an antifungal agent

Journal of Food Science ◽

10.1111/1750-3841.15487 ◽

2020 ◽

Vol 85 (11) ◽

pp. 3920-3926

Author(s):

Luana Izzo ◽

Carlos Luz ◽

Alberto Ritieni ◽

Juanma Quiles Beses ◽

Jordi Mañes ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Antifungal Agent ◽

Potential Application ◽

Fungal Growth ◽

Inhibitory Effect ◽

Sweet Whey

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Inhibitory effect of Lactobacillus plantarum Tennozu-SU2 and Lactococcus lactis subsp. lactis BF1 on Salmonella Typhimurium and Listeria monocytogenes during and post fermentation of soymilk

LWT ◽

10.1016/j.lwt.2018.12.042 ◽

2019 ◽

Vol 102 ◽

pp. 379-384 ◽

Cited By ~ 3

Author(s):

Yutaka Haraguchi ◽

Minori Goto ◽

Takashi Kuda ◽

Mayu Fukunaga ◽

Ayane Shikano ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Salmonella Typhimurium ◽

Lactobacillus Plantarum ◽

Lactococcus Lactis ◽

Inhibitory Effect ◽

Lactococcus Lactis Subsp

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Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production

Toxins ◽

10.3390/toxins11110636 ◽

2019 ◽

Vol 11 (11) ◽

pp. 636 ◽

Cited By ~ 3

Author(s):

Zhao ◽

Zhang ◽

Folly ◽

Chang ◽

Wang ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Aspergillus Flavus ◽

Aflatoxin Production ◽

Inhibitory Effect ◽

Transcriptional Level ◽

Sequencing Data ◽

Inhibitory Effects ◽

Cell Wall Polysaccharides ◽

Colony Forming Units ◽

Insight Into

Lactobacillus plantarum, as a natural bio-preservative, has attracted a great deal of attention in recent years. In this study, 22 L. plantarum strains were tested against the aflatoxin-producing fungus, Aspergillus flavus; strain IAMU80070 showed the highest antifungal activity. At a concentration of 5 × 105 colony-forming units (CFU) mL−1, it completely inhibited A. flavus growth and decreased aflatoxin production by 93%. Furthermore, ultrastructural examination showed that IAMU80070 destroyed the cellular structure of hyphae and spores. To explore the inhibitory effect of IAMU80070 on A. flavus at the transcriptional level, transcriptome data were obtained and subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The aflatoxin biosynthetic process was the most significantly downregulated functional category, while genes implicated in the synthesis and organization of cell wall polysaccharides were upregulated. Quantitative real-time PCR results verified the credibility and reliability of the RNA sequencing data. These results provided insight into the transcriptome of A. flavus in response to the antagonistic effects of L. plantarum IAMU80070.

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Inhibitory Effect of Lactobacillus plantarum CQPC02 Isolated from Chinese Sichuan Pickles (Paocai) on Constipation in Mice

Journal of Food Quality ◽

10.1155/2019/9781914 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Bihui Liu ◽

Xin Yang ◽

Liujin Guo ◽

Jing Zhang ◽

Xianrong Zhou ◽

...

Keyword(s):

Activated Carbon ◽

Lactobacillus Plantarum ◽

Transient Receptor Potential ◽

Receptor Potential ◽

Inhibitory Effect ◽

Lactobacillus Delbrueckii ◽

High Concentration ◽

Inhibition Effect ◽

Probiotic Potential ◽

Transient Receptor

Sichuan pickles (Paocai) are a traditional Chinese fermented vegetable food that is rich in beneficial microorganisms. In this study, the inhibition of constipation by Lactobacillus plantarum CQPC02 (LP-CQPC02) isolated from Sichuan Paocai was studied by establishing a constipation model of mice using activated carbon. The constipation inhibition effect of LP-CQPC02 was determined by observing the defecation of mice, rate of active carbon propulsion, pathological section observation, serum index, and small intestinal mRNA expression. The results showed that LP-CQPC02 inhibited the decrease in body weight caused by constipation in mice. At the same time, LP-CQPC02 also increased the propulsion rate of activated carbon in the small intestine and shortened the time of discharge for the first black stool. LP-CQPC02 increased gastrin (Gas), motilin (MTL), endothelin (ET), and acetylcholinesterase (AChE) in constipated mice, and it also decreased the level of somatostatin (SS). qPCR results showed that LP-CQPC02 upregulated the expression of c-Kit, stem cell factor (SCF), and glial cell-derived neurotrophic factor (GDNF) and downregulated the expression of transient receptor potential cation channel subfamily V member 1 (TRPV1) and inducible nitric oxide synthase (iNOS) in mice with constipation, and a stronger effect was observed with a high concentration of LP-CQPC02. The experimental results showed that LP-CQPC02 has a stronger constipation inhibitory effect than the commonly used commercial Lactobacillus delbrueckii subsp. bulgaricus (LB), and the inhibitory effect of LP-CQPC02 on constipation increases with increasing concentration of LP-CQPC02. Therefore, LP-CQPC02 is a high-quality microbial resource with a good constipation inhibition effect and probiotic potential.

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STUDY THE ANTIMICROBIAL AND ANTIADHESIVE ACTIVITY OF PURIFIED BIOSURFACTANT PRODUCED FROM LACTOBACILLUS PLANTARUM AGAINST PATHOGENIC BACTERIA

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v52i5.1457 ◽

2021 ◽

Vol 52 (5) ◽

pp. 1194-1206

Author(s):

A . A. Jameel ◽

N. H. Haider

Keyword(s):

Surface Tension ◽

Lactobacillus Plantarum ◽

Pathogenic Bacteria ◽

Inhibition Zone ◽

Lower Surface ◽

Solvent System ◽

Inhibitory Effect ◽

Clinical Samples ◽

Adhesion Activity ◽

Natural Media

This study was aimed to purification the biosurfactant that produced from Lactobacillus plantarum isolated from clinical samples of iraqi healthy women by column chromatography through silica gel column (3.5 × 30 cm) using solvent system (chloroform : methanol, 2:1) and characterization the purified product by Thin layer chromatography (TLC), Fourier Transform Infrared Red spectroscopy (FTIR) and Gas chromatography (GC) mass then evaluated its antibacterial and anti-adhesive activity. The results shown the higher emulsification activity (E24%= 63) and lower the surface tension to 23 mN/m in synthetic MSM, while the natural media gave (E24% =71) and lower surface tension to 19 mN/m. The using TLC technique indicated presence of lipopeptide in the biosurfactant with Rf value = 0.82. Antibacterial and antiadhesion activities were evaluated against some pathogenic bacteria, including Pseudomonas aureginosa and Staphylococcus aureus. The results showed a higher inhibitory effect of biosurfactant at concentration 200 mg/ml on S. aureus and P. aeruginosa with the inhibition zone 27 mm and 33 mm respectively in BCDFTM media. While purified biosurfactant with concentration 200 mg/ml produced in MSM media had an effect on S. aureus and P. aeruginosa with the inhibition zone 21 mm, and 18 mm respectively. The anti-adhesion activity of purified biosurfactant against p.aeruginosa ranging from (34.70 ± 5.256) to (11.7 ± 9.7) produced from natural media BCDFTM and was higher than the anti-adhesion activity of purified biosurfactant against the same pathogen ranging from (46.95 ± 7.37) to (20.15 ± 2.805) produced in MSM.

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Kisameet Clay Exhibits Potent Antibacterial Activity against the ESKAPE Pathogens

mBio ◽

10.1128/mbio.01842-15 ◽

2016 ◽

Vol 7 (1) ◽

Cited By ~ 17

Author(s):

Shekooh Behroozian ◽

Sarah L. Svensson ◽

Julian Davies

Keyword(s):

Antibacterial Activity ◽

Nosocomial Infections ◽

Antimicrobial Agents ◽

Clinical Investigation ◽

Therapeutic Option ◽

Multidrug Resistant ◽

Inhibitory Effect ◽

Natural Clay ◽

Mortality And Morbidity ◽

Natural Medicine

ABSTRACT The ESKAPE ( Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , and Enterobacter species) pathogens cause an increasing number of nosocomial infections worldwide since they escape the inhibitory effect of the available antibiotics and the immune response. Here, we report the broad-spectrum and potent antibacterial activity of Kisameet clay, a natural clay mineral from British Columbia, Canada, against a group of multidrug-resistant ESKAPE strains. The results suggest that this natural clay might be developed as a therapeutic option for the treatment of serious infections caused by these important pathogens. IMPORTANCE More than 50 years of misuse and overuse of antibiotics has led to a plague of antibiotic resistance that threatens to reduce the efficacy of antimicrobial agents available for the treatment of infections due to resistant organisms. The main threat is nosocomial infections in which certain pathogens, notably the ESKAPE organisms, are essentially untreatable and contribute to increasing mortality and morbidity in surgical wards. The pipeline of novel antimicrobials in the pharmaceutical industry is essentially empty. Thus, there is a great need to seek for new sources for the treatment of recalcitrant infectious diseases. We describe experiments that demonstrate the efficacy of a “natural” medicine, Kisameet clay, against all of the ESKAPE strains. We suggest that this material is worthy of clinical investigation for the treatment of infections due to multidrug-resistant organisms.

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