scholarly journals STUDY THE ANTIMICROBIAL AND ANTIADHESIVE ACTIVITY OF PURIFIED BIOSURFACTANT PRODUCED FROM LACTOBACILLUS PLANTARUM AGAINST PATHOGENIC BACTERIA

2021 ◽  
Vol 52 (5) ◽  
pp. 1194-1206
Author(s):  
A . A. Jameel ◽  
N. H. Haider
Keyword(s):  
Surface Tension ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Lower Surface ◽  
Solvent System ◽  
Inhibitory Effect ◽  
Clinical Samples ◽  
Adhesion Activity ◽  
Natural Media

This study was aimed to purification the biosurfactant that produced from Lactobacillus plantarum isolated from clinical samples of iraqi healthy women by column chromatography through silica gel column (3.5 × 30 cm) using solvent system (chloroform : methanol, 2:1) and characterization the purified product by Thin layer chromatography (TLC), Fourier Transform Infrared Red spectroscopy (FTIR) and Gas chromatography (GC) mass then evaluated its antibacterial and anti-adhesive activity. The results shown the higher emulsification activity (E24%= 63) and lower the surface tension to 23 mN/m in synthetic MSM, while the natural media gave (E24% =71) and lower surface tension to 19 mN/m. The using TLC technique indicated presence of lipopeptide in the biosurfactant with Rf value = 0.82. Antibacterial  and antiadhesion activities were evaluated against some pathogenic  bacteria, including Pseudomonas aureginosa and Staphylococcus aureus. The results showed a higher inhibitory effect of biosurfactant  at concentration 200 mg/ml on  S. aureus and P. aeruginosa with the inhibition zone 27 mm and 33 mm respectively in BCDFTM media. While purified  biosurfactant with concentration 200 mg/ml produced in MSM media had an effect on S. aureus and P. aeruginosa with the inhibition zone 21 mm, and 18 mm respectively. The anti-adhesion activity of purified biosurfactant against p.aeruginosa ranging from (34.70 ± 5.256) to (11.7 ± 9.7) produced from natural media BCDFTM  and was higher than the anti-adhesion activity of purified biosurfactant against the same pathogen ranging from (46.95 ± 7.37) to (20.15 ± 2.805)  produced in MSM.

scholarly journals Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 85
Author(s):  
Novi Permata Sari ◽  
Rafika Sari ◽  
Eka Kartika Untari
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Heating Temperature ◽  
Lactobacillus Brevis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Activity Test ◽  
Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum. 

scholarly journals The Inhibitory Effect of Lactobacillus acidophilus and Lactobacillus plantarum against Candida albicans Associated with Denture Stomatitis

2015 ◽  
Vol 12 (3) ◽  
pp. 479-484
Author(s):  
Baghdad Science Journal
Keyword(s):  
Candida Albicans ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Acidophilus ◽  
Solid Medium ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Denture Stomatitis ◽  
Inhibition Effect

In this study Candida speices was diagnosed in 26 swab samples from patients with denture stomatitis , investigates the antagonism activity of Lactobacillus was investigated against the yeast of Candida albicans in vitro.Results revealed that The inhibition effect of Lactic Acid Bacteria against C.albicans was examined in solid medium, L.plantarum gave higher inhibition average 11mm followed by L.acidophillus with average 9 mm and, L.fermentum , L.casei with averages 7 mm. Whereas the filtrates, the highest inhibition zone were 20 and 16 mm by L. plantarum and L.acidophillus, respectively.

scholarly journals Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis

2017 ◽  
Vol 18 (4) ◽  
pp. 312-316 ◽  
Author(s):  
Aarti A Bohora ◽  
Sharad R Kokate
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Indicator Strain ◽  
Oral Bacteria ◽  
Diffusion Method

ABSTRACT Introduction The main goal of endodontics is the prevention of apical periodontitis. This is due to the presence of persistent pathogenic microorganisms, such as Enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. Lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. This study explores shifting the established paradigm of endodontic treatment, which has focused on eliminating all bacteria from the canal system and on elimination of the problematic bacteria through introducing probiotics. A preliminary work was performed to evaluate the possible effectiveness of probiotics in preventing the growth of E. faecalis. Materials and methods Two probiotic cultures Lactobacillus plantarum ATCC 8041 and Lactobacillus rhamnosus ATCC 7408 were selected to check their antimicrobial activity against E. faecalis ATCC 29212 by two methods: agar cup/well diffusion method and deferred antagonism test. Agar cup method A total of 0.5 mL of requisite test pathogen culture was inoculated into 20 mL of molten sterile Mueller and Hinton agar and cooled to 45 ± 2°C. Circular wells of diameter 10 mm were punched in each of the poured plates. Appropriately diluted test samples were added to the above-punched wells. The plates were incubated upright position at 37°C for 24 hours in aerobic conditions. Postincubation, zone of inhibition was measured. The cell-free supernatant of Lactobacillus species was also evaluated for antimicrobial activity. Deferred antagonism test The test probiotic strain was standardized to 0.1 optical density (OD) at 600 nm and inoculated in a 1 cm wide diametric streak across the surface of trypticase soy agar + yeast extract + calcium carbonate (TSYCa) agar using a sterile cotton swab. Then, the plate agar containing the test strain was incubated at 37°C for 24 hours under aerophilic condition and then standardized to 0.1 OD at 600 nm for overnight (18 hours, 37°C). A purified culture of indicator strain (pathogen) was streaked at right angles to the line of original producer growth. Postincubation plates were observed for the inhibition zone width of the indicator strain. Results Under the conditions of this study, Lactobacilli had an inhibitory effect on the growth of E. faecalis by agar cup method but not by deferred antagonism test. Conclusion This pilot study demonstrated that probiotics show a potential in root canal therapy. Clinical Significance If probioitcs are effective against endodontic pathogens, they can be potentially used as intracanal medicaments. This will be a novel concept of introducing bacteriotherapy in endodontics and replacing pathogenic bacteria by healthy bacteria, normal flora. How to cite this article Bohora AA, Kokate SR. Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis. J Contemp Dent Pract 2017;18(4):312-316.

scholarly journals The effect of a seed coating with Origanum vulgare essential oil on Clavibacter michiganensis subsp. michiganensis

2021 ◽  
Author(s):  
Meral Yılmaz ◽  
Ömür Baysal ◽  
Ragıp Soner Silme
Keyword(s):  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Seed Quality ◽  
Seed Transmission ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Clavibacter Michiganensis ◽  
Origanum Vulgare ◽  
Ligand Interaction ◽  
Clavibacter Michiganensis Subsp

The study aimed to evaluate the efficacy of a seed film coating with Origanum vulgare Linnaeus essential oil (EO) against Clavibacter michiganensis subsp. michiganensis (Cmm) on tomatoes. Tomato seeds (cv. Rio Grande) coated with different doses of EO derived from O. vulgare were inoculated with Cmm (1.8 × 10<sup>8</sup> CFU/mL). O. vulgare's EO showed a remarkable inhibition effect on the Cmm growth. The EO effect against Cmm was determined based on the parameters, such as the inhibition zone and bacterial population in a seed. The GC-MS analysis of EO showed that carvacrol is the major component (at 74.05%), which may inhibit the bacterial growth. Later, we have expanded our studies to determine the inhibitory effect of the EO's mode of action on the pathogenic bacteria with a molecular docking analysis based on the molecular protein-ligand interaction. The results showed that carvacrol has a strong interaction with the bacterial expansin protein (PDB 4JJO) of Cmm and the qPCR analyses confirmed the effect of the O. vulgare treatment against Cmm. This original approach has the prominent potential to prevent seed transmission of Cmm for seed quality in the world, suggesting a method for paving the way for Cmm disease management.

scholarly journals Estimating the inhibitory effect of Lactobacillus isolated from different sources on some pathogens of urogenital infections in women group

2009 ◽  
Vol 3 (2) ◽  
pp. 65-73
Author(s):  
Bushra J . Mohamed ◽  
Amina N. AL. Thwani
Keyword(s):  
Human Milk ◽  
Pathogenic Bacteria ◽  
Klebsiella Oxytoca ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Cow Milk ◽  
Lactobacillus Species ◽  
Urogenital Infections ◽  
Different Sources ◽  
Lactobacillus Spp

This study was aimed to identify of pathogenic organism isolated from urogintal tract and estimate the effect of Lactobacillus which isolated from different sources on the growth of these pathogens including (Candida albicans, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Gardnerella viginals, Klebsiella oxytoca ,Proteus mirabilis). The results showed that Lactobacillus which was isolated from vagina on solid medium was effective against pathogenic isolates more than the Lactobacillus species isolated from yoghurt, cow milk, human milk with inhibition zone (12-16) mm, while the highs inhibitory effect of Lactobacillus isolated from vagina in liquid media with inhibition zone reached to(18)mm. Adversely, lowest inhibitory effect was shown with supernatant of Lactobacillus spp isolated from human milk with inhibition zone reached to(11)mm comparison with Lactobacillus spp isolated from yoghurt and cow milk with inhibition zone (13-15) mm. Also the result revealed that Lactobacillus spp isolated from vagina, human milk, cow milk and yoghurt by overlay method had no effect on pathogenic bacteria but high effect was shown only with the vaginal Lactobacillus isolates on C .albicans.

scholarly journals Investigating the Inhibitory Effect of Silver Nanoparticles against Some Species of Candida and Pathogenic Bacteria

2020 ◽  
Vol 4 (1) ◽  
pp. 32-36
Author(s):  
Alaa M. Hasan ◽  
Sura M. Abdul Majeed ◽  
Rusol M. Al-Bahrani
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Candida Guilliermondii ◽  
Candida Krusei ◽  
Maximum Inhibition ◽  
Bacteria And Fungi ◽  
Candida Zeylanoides

Silver nanoparticles synthesized from aqueous extract of mushroom Pleurotus ostreatus exhibited inhibitory effect at the concentration of 12.5, 25, 50, and 100 mg/ml against some pathogenic bacteria and fungi such as Candida albicans, Candida guilliermondii, Candida krusei, Candida zeylanoides, Geotrichum klebahnii, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The maximum inhibition zone was observed against C. zeylanoides at the concentration of 100 mg/ml was 24.5 mm, while the minimum inhibition zone was observed against Geotrichum at the concentration of 25 mg/ml was 8 mm and the concentration of 12.5 mg/ml was not effective against some species.

Comparison 0f effect of Antibiotics,Natural Honey and plant ExtractsonE.coliisolated from different clinical cases

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Amal Talib Al-Sa'ady
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Allium Sativum ◽  
High Sensitivity ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Clinical Samples ◽  
Tract Infections ◽  
Natural Honey

200 different clinical samples were collected from patients with Burns infections, wound infections, urinary Tract Infections & diarrhea for a period from March to August, 2017. Total of 403 bacterial isolates were identified, the gram negative bacteria E.coli represented329 (48.9%). The percentages of E.coliisolatesare different depending on isolation sources, urinary Tract Infections have the highest rate of isolation (72.2%). Bacterial sensitivity against10different antibiotics were tested, all isolates showed full resistance againstPenicillins & Cephalosporins while they have high sensitivity for Nitrofurantion. The other antibiotics have different effects depending on the isolation sources. Natural Honey has high antibacterial activity for E.coliwithout any influence withisolation sources with MIC,15%. Antibacterial activity ofthe EthanolicExtracts (95%) for four plants: Allium sativum,Paganum harmala,Nigella sativum & Myrtus communs. All of this have high inhibitory effect onE.coliisolates without any influence of the isolation sources, but Allium sativum extract has the highest effect with inhibition zone diameters (20.1-32.0) mm.

Isolation, identification and characterization of Lactobacillus plantarum from camel milk and its antagonist effect against diarrheal bacteria.

2018 ◽  
Vol 30 (4) ◽  
pp. 283 ◽  
Author(s):  
Meriem Toualbia, Abd ElKader Delmi Bouras ◽  
Malika Koiche, Mohamed Kerkoud
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Bioactive Molecules ◽  
Camel Milk ◽  
Fecal Samples ◽  
Infantile Diarrhea ◽  
Rdna Sequencing

The main objective of this study is to define probiotic bacteria efficiency against bacterial infantile diarrhea. Lactic acid bacteria (LAB) was used as a natural resource and extracted of camel milk from southern Algeria. The isolation was followed by molecular identification using the 16S rDNA sequencing method. One hundred and fifty fecal samples were collected over 18 months from children suffering from diarrhea and aged 2 years or under. Identification results reveal the isolated lactic bacteria as Lactobacillus plantarum. In the other side, a total of 120 fecal samples were positive for bacterial growth, these pathogenic bacteria were identified as: Escherichia fergusonii (92%), Salmonella enterica subsp. Diarizonae (7.33%) and Proteus mirabilis (0.66%). The inhibitory effect study of lactic acid bacteria on pathogenic bacteria shows varying effects of L. plantarum in relation to the various pathogenic isolates, mentioning that the most important effect was expressed against Escherichia fergusonii with 22 mm. All the results allow us to classify that camel's milk is a natural source rich in lactic acid bacteria, in particular L. plantarum, with an inhibitory ability against the pathogenic bacteria responsible for diarrhea. Therefore L. plantarum produces bioactive molecules responsible for this effect.

Inhibition of Pathogenic Bacteria by Camel's Milk: Relation to Whey Lysozyme and Stage of Lactation

1984 ◽  
Vol 47 (11) ◽  
pp. 838-840 ◽  
Author(s):  
ELIE K. BARBOUR ◽  
NASSIM H. NABBUT ◽  
WAYNE M. FRERICHS ◽  
HABEEB M. AL-NAKHLI
Keyword(s):  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Shigella Dysenteriae ◽  
Milk Samples ◽  
Stage Of Lactation ◽  
California Mastitis Test ◽  
Two Samples ◽  
Test Organisms ◽  
Camel's Milk

Twenty (10%) of 200 milk samples collected from different individual camels inhibited growth of one or more of six pathogenic test organisms in a filter-paper disk assay. The milk samples with inhibitory properties scored zero in the California Mastitis Test. The percentages of milk samples which inhibited Clostridium perfringens, Staphylococcus aureus, Shigella dysenteriae and Salmonella typhimurium were 7.5, 4.0, 2.0 and 1%, respectively. None of the 200 samples inhibited Bacillus cereus or Escherichia coli. Five milk samples inhibited growth of two test organisms and two samples inhibited growth of three organisms. Inhibition zone diameters ranged from 13.5 mm to 22.0 mm (average of 15 mm) using a disk of 12.5 mm in diameter. The average amount of lysozyme in the whey of 58 samples of camel's milk was 288 μg/100 ml. The average lysozyme content of the 20 samples showing growth inhibition of one or more test organisms was 648 μg/100 ml, significantly higher (P&lt;0.01) than the average in the 38 samples (62.6 μg/100 ml) that had no inhibitory effect. There was a negative correlation (r= −0.669) between lysozyme content in the whey of milk samples (y) and the stage of lactation of the camels (x) with the following regression equation: y = −2.86x + 665.8.

scholarly journals Effect of Lactobacillus salivarius supernatant against growth and biofilm formation of some pathogenic microorganisms

2016 ◽  
Vol 13 (2) ◽  
pp. 247-252
Author(s):  
Baghdad Science Journal
Keyword(s):  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Pathogenic Microorganisms ◽  
Lactobacillus Salivarius ◽  
Inhibition Effect ◽  
Probiotic Lactobacillus ◽  
Diffusion Assay ◽  
Klebsiella Spp

The Inhbititory effect of cocentrated and non-cocentrated supernatant of the probiotic Lactobacillus salivarius against growth of some potential pathogenic microorganisms which included Pseudomonas eruginosa, Klebsiella spp, Escherichia coli and Candida albicans. The results were diffusion assay revealed that concentrated and non-concentrated supernatant had inhitory effect against pathogenic bacteria with inhibition zone renged between 13-17mm while inhibition effect of concentrated supernatant against C.albicans was inhibition zone 8mm. On the other hand, the effect of these suprnatant against biofilm formation of the tested microorganisms was studied. The result showed that the concentrated supernatant had inhibitory effect on biofilm formation for all tested microorganisms with percentage (28-29)% against tested bacteria and (23)% against C.albicans.

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