The probiotic Lactobacillus fermentum 296 attenuates cardiometabolic disorders in high fat diet-treated rats

2019 ◽  
Vol 29 (12) ◽  
pp. 1408-1417 ◽  
Author(s):  
Raíssa G.S. Cavalcante ◽  
Thatyane M.R. de Albuquerque ◽  
Micaelle O. de Luna Freire ◽  
Georgianna A.H. Ferreira ◽  
Lucas A. Carneiro dos Santos ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Lactobacillus Fermentum ◽  
High Fat ◽  
Cardiometabolic Disorders ◽  
Probiotic Lactobacillus

scholarly journals Lactobacillus fermentum CQPC07 attenuates obesity, inflammation and dyslipidemia by modulating the antioxidant capacity and lipid metabolism in high-fat diet induced obese mice

2020 ◽  
Author(s):  
Ya Wu ◽  
Xueya Li ◽  
Fang Tan ◽  
Xianrong Zhou ◽  
Jianfei Mu ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Lipid Metabolism ◽  
Antioxidant Capacity ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Lactobacillus Fermentum ◽  
Lipoprotein Cholesterol ◽  
High Fat

Abstract Backgroud: Obesity is an epidemic disease in the world, the treatment and prevention of obesity methods have gained great attention. Lactobacillus is the main member of probiotics, and the physiological activity of it is specific to different strains. This study systematically explored the anti-obesity effect and possible mechanism of Lactobacillus fermentum CQPC07 (LF-CQPC07), which was isolated from pickled vegetables.Results: LF-CQPC07 effectively controlled the weight gain of mice caused by a high-fat diet. The results of pathological sections indicated that LF-CQPC07 alleviated hepatocyte damage and fat accumulation in adipocytes. The detection of biochemical indictors revealed that LF-CQPC07 decreased the levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), and increased the level of high-density lipoprotein cholesterol (HDL-C). Additionally, LF-CQPC07 caused the decrease in the amounts of inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), IL-6, and interferon-γ (IFN-γ), and the increase in the amounts of the anti-inflammatory cytokines IL-10 and IL-4. LF-CQPC07 also decreased the amounts of alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP). Confirmed by qPCR, LF-CQPC07 enhanced the mRNA expression of catalase (CAT), gamma glutamylcysteine synthetase 1 (GSH1), copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), and glutathione peroxidase (GSH-Px). It also increased the mRNA expression levels of carnitine palmitoyltransferase 1 (CPT1), peroxisome proliferator-activated receptor alpha (PPAR-α), lipoprotein lipase (LPL), and cholesterol 7 alpha hydroxylase (CYP7A1), and decreased that of PPAR-γ and CCAAT/enhances binding protein alpha (C/EBP-α) in the liver of mice.Conclusion: this research confirmed that LF-CQPC07 is capable of ameliorating obesity, improving hyperlipemia, and alleviating chronic low-grade inflammation and liver injury accompanied with obesity. Its mechanism may be the regulation of antioxidant capacity and lipid metabolism. Therefore, LF-CQPC07 has enormous potential to serve as a potential probiotic for the prevention or treatment of obesity.

scholarly journals Effect of Yogurt Fermented by Lactobacillus Fermentum TSI and L. Fermentum S2 Derived from a Mongolian Traditional Dairy Product on Rats with High-Fat-Diet-Induced Obesity

Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 594
Author(s):  
Won-Young Cho ◽  
Go-Eun Hong ◽  
Ha-Jung Lee ◽  
Su-Jung Yeon ◽  
Hyun-Dong Paik ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Dairy Product ◽  
Hdl Cholesterol ◽  
Blood Lipid ◽  
Normal Diet ◽  
Lactobacillus Fermentum ◽  
Sprague Dawley ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Saline Administration

This study aimed to investigate the metabolic effect of yogurt fermented by Lactobacillus fermentum TSI and S2 isolated from a Mongolian traditional dairy product on rats with high-fat-diet-induced obesity. Quality characteristics of yogurt fermented by commercial starter (CON), L. fermentum TSI2 (TSI2 group), L. fermentum S2 (S2 group), and mixed TSI2 and S2 strains at 1:1 (MIX group), were verified. Six-week-old male Sprague-Dawley rats were divided into five groups and administered the following diets: group NOR, normal diet with oral saline administration; group HF, high-fat diet (HD) with oral saline administration; group TSI, HD and L. fermentum TSI-fermented yogurt; group S2, HD and L. fermentum S2-fermented yogurt; and group MIX, HD and MIX-fermented yogurt. After eight weeks, the HD groups displayed significantly increased body weight and fat, serum cholesterol, and abdominal adipose tissue levels. However, serum HDL cholesterol levels were higher, triglyceride levels were lower, and abdominal adipocytes were smaller in the TSI and S2 groups than in the HF group. These results indicate that L. fermentum TSI reduces abdominal fat and improves blood lipid metabolism in HD-induced obese rats.

scholarly journals Lactobacillus fermentum CECT5716 ameliorates high fat diet-induced obesity in mice through modulation of gut microbiota dysbiosis

2021 ◽  
pp. 105471
Author(s):  
Jose Alberto Molina-Tijeras ◽  
Patricia Diez-Echave ◽  
Teresa Vezza ◽  
Laura Hidalgo-García ◽  
Antonio Jesús Ruiz-Malagón ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Lactobacillus Fermentum ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity In Mice ◽  
Gut Microbiota Dysbiosis

scholarly journals Lactobacillus fermentum CECT5716 ameliorates high fat diet-induced obesity in mice through modulation of gut microbiota dysbiosis.

2020 ◽  
Author(s):  
Jose Alberto Molina Tijeras ◽  
Patricia Diez Echave ◽  
Teresa Vezza ◽  
Laura Hidalgo Garcia ◽  
Antonio Jes s Ruiz Malag n ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Lactobacillus Fermentum ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity In Mice ◽  
Gut Microbiota Dysbiosis

scholarly journals Galangin Resolves Cardiometabolic Disorders Through Modulation of AdipoR1/COX-2/ NF-κB Expressions in High-Fat Diet Fed Rats

2021 ◽  
Author(s):  
Patoomporn Prasatthong ◽  
Sariya Meephat ◽  
Siwayu Rattanakanokchai ◽  
Juthamas Khamseekaew ◽  
Sarawoot Bunbupha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Biological Activities ◽  
Metformin Treatment ◽  
High Fat ◽  
Oxidative Stress Biomarkers ◽  
Impaired Liver Function ◽  
Cardiometabolic Disorders ◽  
Cox 2 ◽  
And Oxidative Stress

Abstract Background: Rats fed with a high-fat diet exerts signs of cardiometabolic disorders. Galangin is a natural flavonoid mainly isolated from honey and Alpinia officinarum Hance and has various biological activities. This study evaluated whether galangin could alleviate cardiometabolic disorders, inflammation and oxidative stress in a high-fat diet fed rats. Methods: Male Sprague-Dawley rats were fed with a high-fat diet plus 15% fructose in drinking water for 4 months to induced signs of metabolic syndrome (MS), and they were treated with galangin at a dose 25 or 50 mg/kg or metformin at a dose 100 mg/kg or vehicle for the last four weeks. All data were expressed as mean ± S.E.M. Data were analyzed by one-way analysis of variance followed by Tukey’s post-hoc test for multiple comparisons analysis.Results: Rats fed with a high-fat diet had impaired glucose tolerance, insulin resistance, hyperglycemia, hypertrophy of adipocytes, impaired liver function and hypertension. These signs of MS were alleviated by galangin or metformin treatment (p<0.05). Galangin or metformin alleviated cardiac dysfunction and remodeling induced by a high-fat diet in rats (p<0.05). Tumor necrosis factor-α and interleukin-6 concentrations and expression were high in plasma and cardiac tissue in MS rats, and these inflammatory markers were suppressed by galangin or metformin treatment (p<0.05). Galangin alleviated a high-fat diet induced low levels of adiponectin in rats. Galangin or metformin decreased oxidative stress biomarkers, aortic superoxide generation and plasma and cardiac MDA levels, and raised endogenous antioxidant enzume activities, catalase, and superoxide dismutase, in MS rats (p<0.05). Downregulation of adiponectin receptor1 (AdipoR1) and cyclooxygenase-2 (COX-2) as well as upregulation of nuclear factor kappa B (NF-κB) expression were observed in MS rats. These alterations of protein expressions were recovered in MS rats treated with galangin or metformin. Conclusions: Galangin reduced cardiometabolic disorders in high-fat diet induced MS rats. The underlying mechanisms might be relevant to suppression of inflammation and oxidative stress and restoration of AdipoR1/COX-2/NF-κB expression.

scholarly journals Lactobacillus fermentum CQPC07 attenuates obesity, inflammation and dyslipidemia by modulating the antioxidant capacity and lipid metabolism in high-fat diet induced obese mice

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Ya Wu ◽  
Xueya Li ◽  
Fang Tan ◽  
Xianrong Zhou ◽  
Jianfei Mu ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Lipid Metabolism ◽  
Antioxidant Capacity ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Lactobacillus Fermentum ◽  
Lipoprotein Cholesterol ◽  
High Fat

Abstract Background Obesity is an epidemic disease in the world, the treatment and prevention of obesity methods have gained great attention. Lactobacillus is the main member of probiotics, and the physiological activity of it is specific to different strains. This study systematically explored the anti-obesity effect and possible mechanism of Lactobacillus fermentum CQPC07 (LF-CQPC07), which was isolated from pickled vegetables. Results LF-CQPC07 effectively controlled the weight gain of mice caused by a high-fat diet. The results of pathological sections indicated that LF-CQPC07 alleviated hepatocyte damage and fat accumulation in adipocytes. The detection of biochemical indictors revealed that LF-CQPC07 decreased the levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), and increased the level of high-density lipoprotein cholesterol (HDL-C). Additionally, LF-CQPC07 caused the decrease in the amounts of inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), IL-6, and interferon-γ (IFN-γ), and the increase in the amounts of the anti-inflammatory cytokines IL-10 and IL-4. LF-CQPC07 also decreased the amounts of alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP). Confirmed by qPCR, LF-CQPC07 enhanced the mRNA expression of catalase (CAT), gamma glutamylcysteine synthetase 1 (GSH1), copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), and glutathione peroxidase (GSH-Px). It also increased the mRNA expression levels of carnitine palmitoyltransferase 1 (CPT1), peroxisome proliferator-activated receptor alpha (PPAR-α), lipoprotein lipase (LPL), and cholesterol 7 alpha hydroxylase (CYP7A1), and decreased that of PPAR-γ and CCAAT/enhancer binding protein alpha (C/EBP-α) in the liver of mice. Conclusion This research confirmed that LF-CQPC07 is capable of ameliorating obesity, improving hyperlipemia, and alleviating chronic low-grade inflammation and liver injury accompanied with obesity. Its mechanism may be the regulation of antioxidant capacity and lipid metabolism. Therefore, LF-CQPC07 has enormous potential to serve as a potential probiotic for the prevention or treatment of obesity.

scholarly journals Lactobacillus fermentum MCC2759 and MCC2760 Alleviate Inflammation and Intestinal Function in High-Fat Diet-Fed and Streptozotocin-Induced Diabetic Rats

2021 ◽  
Author(s):  
Ann Catherine Archer ◽  
Serva Peddha Muthukumar ◽  
Prakash Motiram Halami
Keyword(s):  
High Fat Diet ◽  
Quantitative Polymerase Chain Reaction ◽  
Intestinal Barrier ◽  
Diabetic Rats ◽  
Lactobacillus Fermentum ◽  
Oral Glucose ◽  
Low Grade ◽  
High Fat ◽  
Insulin Levels ◽  
Markers Of Inflammation

AbstractThe growing incidence of type 2 diabetes and obesity has become a worldwide crisis with increased socio-economic burden. Changes in lifestyle and food habits resulting in dysbiosis of the gut microbiota and low-grade inflammation are linked to the rising incidence. The aim of this study was to investigate the effects of potential probiotic Lactobacillus fermentum MCC2759 and MCC2760 on intestinal markers of inflammation using a high-fat diet (HFD)-fed model and a streptozotocin (STZ)-induced diabetic model. Lact. fermentum administration showed improved oral glucose tolerance compared with the model controls of HFD (AUC 1518) and STZ (628.8). Plasma insulin levels improved in the Lact. fermentum treated groups of HFD + MCC2759 (129 ± 4.24 pmol/L) and HFD + MCC2760 (151.5 ± 9.19 pmol/L) in HFD study, while in STZ diabetic study, the insulin levels were normalized with Lact. fermentum administration, for D + MCC2759 (120.5 ± 7.77) and D + MCC2760 (138 ± 5.65 pmol/L) groups. The results showed reduction in inflammatory tone in liver, muscle, and adipose tissues of rats in both models with stimulation of anti-inflammatory IL-10 by real-time quantitative polymerase chain reaction. Additionally, the potential probiotic cultures also displayed normalization of markers related to intestinal barrier integrity (ZO-1), TLR-4 receptor, and insulin sensitivity (GLUT-4, GLP-1, adiponectin). Thus, the results suggest that Lact. fermentum could act as potential probiotic for lifestyle-related disorders such as obesity, diabetes, and metabolic syndrome as both prophylactic and adjunct therapies.

scholarly journals Oral administration of Lactobacillus fermentum CRL1446 improves biomarkers of metabolic syndrome in mice fed a high-fat diet supplemented with wheat bran

2020 ◽  
Vol 11 (5) ◽  
pp. 3879-3894
Author(s):  
M. Russo ◽  
A. Marquez ◽  
H. Herrera ◽  
C. Abeijon-Mukdsi ◽  
L. Saavedra ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Oral Administration ◽  
Intestinal Microbiota ◽  
Wheat Bran ◽  
High Fat Diet ◽  
Lactobacillus Fermentum ◽  
Feruloyl Esterase ◽  
High Fat ◽  
Metabolic Biomarkers

This work evaluated the effect of oral administration of Lactobacillus fermentum CRL1446, feruloyl esterase producing, on metabolic biomarkers and intestinal microbiota of high fat diet-induced metabolic syndrome mice and supplemented with wheat bran.

Sign in / Sign up

Export Citation Format

Share Document