scholarly journals The potential impact of new generation transgenic methods on creating rabbit models of cardiac diseases

2016 ◽  
Vol 121 (2) ◽  
pp. 123-130 ◽  
Author(s):  
Z. Bősze ◽  
P. Major ◽  
I. Baczkó ◽  
K.E. Odening ◽  
L. Bodrogi ◽  
...  
2014 ◽  
Vol 25 ◽  
pp. iv359
Author(s):  
K. Kulig ◽  
C. De Moor ◽  
B. Korytowsky ◽  
M. Halperin ◽  
M. Danese

Parasitology ◽  
2010 ◽  
Vol 137 (9) ◽  
pp. 1291-1302 ◽  
Author(s):  
RICHARD A. SCHELTEMA ◽  
SASKIA DECUYPERE ◽  
RUBEN T'KINDT ◽  
JEAN-CLAUDE DUJARDIN ◽  
GRAHAM H. COOMBS ◽  
...  

SUMMARYThe post-genomics era has provided researchers with access to a new generation of tools for the global characterization and understanding of pathogen diversity. This review provides a critical summary of published Leishmania post-genomic research efforts to date, and discusses the potential impact of the addition of metabolomics to the post-genomic toolbox. Metabolomics aims at understanding biology by comprehensive metabolite profiling. We present an overview of the design and interpretation of metabolomics experiments in the context of Leishmania research. Sample preparation, measurement techniques, and bioinformatics analysis of the generated complex datasets are discussed in detail. To illustrate the concepts and the expected results of metabolomics analyses, we also present an overview of comparative metabolic profiles of drug-sensitive and drug-resistant Leishmania donovani clinical isolates.


Subject Changing undercurrents in Algeria society and their potential impact. Significance The decline in oil prices and reduced government revenues have brought uncertainty about the country's future back to the surface. Algerian media has been reporting daily on the economic effects of decreased oil and gas revenues, which account for 60% of total budget revenue in the past three years. Coupled with instability in neighbouring Libya and Mali, and rare domestic unrest in Ghardaia, Algiers is keen to give the impression that it is addressing the nation's problems. However, it is has struggled to respond to these pressures as the country's underlying fundamentals are changing. Impacts The ruling elite will avoid cutting subsidies despite the financial pressures. Entrepreneurs may gain increasing their influence in politics. A change in the balance of power away from the army and towards the president and business elite could undercut state security.


2022 ◽  
pp. 19-41
Author(s):  
Yunus Topsakal ◽  
Onur Icoz ◽  
Orhan Icoz

Technology has transformed the tourism industry over time, and today, this transformation has accelerated with digitalization and Industry 4.0. With the application of new generation technologies that shape Industry 4.0 to the tourism industry, the concept of smart tourism has entered the literature and digitalization has accelerated in tourism. It can be said that digital transformation has had an impact on tourist experiences. In this context, this chapter aims to describe the potential impact of digital transformation on the tourist experience. For this purpose, the following topics and concepts will be studied: uses of Industry 4.0 technologies in tourism industry, digital transformation, tourist experiences, and effects of digital transformation on tourist experience.


Itinerario ◽  
2000 ◽  
Vol 24 (3-4) ◽  
pp. 9-28 ◽  
Author(s):  
Jan Luiten Van Zanden

Between 1995 and 2000 a number of synthetic studies on the economic history of Asia in the Early Modern Period were published which have changed – or should change – our ideas and perceptions of the ‘rise of the west’ and the parallel ‘decline of the east’ in a fundamental way. The potential impact of these studies is comparable to that of a previous brief spell of brilliance in our profession, the early 1970s, with the pioneering publications by, amongst others, Wallerstein, Brenner, and North and Thomas. Whereas these studies proposed fundamentally new views on the long term dynamics of the ‘rise of the west’, and concentrated heavily on the economic and socio-political history of Europe (albeit sometimes within a ‘world system perspective’), the new generation of innovative works focuses on a new analysis of the economic history of parts of Asia - on China and India in particular. Much of the detailed empirical research on which this revisionism is based, was done before the books of Goody, Frank, Wong, Pomeranz, and Lee and Wang were published, and forerunners of the revisionism can be identified. But only now the movement has created a clear set of hypotheses that challenges the accepted wisdom about die economic and institutional contrasts between both sides of the Eurasian Continent.


2013 ◽  
Vol 633 ◽  
pp. 169-180 ◽  
Author(s):  
Olga Troynikov ◽  
Nazia Nawaz ◽  
Irena Yermakova

The level of protective material performance attributes are well defined and highly regulated, however the attributes related to the thermo physiological comfort of materials are not. In this chapter, the application of new materials to firefighting protective clothing systems used in extreme heat is addressed, with a focus on thermo physiological comfort. The new generation of protective textile materials and their structures are evaluated through use of both objective laboratory testing and mathematical modeling methods. In addition, 3D body imaging technology is utilized to demonstrate a method of assessing the fit of protective garments and its potential impact on the thermal status of the wearer. The proposed engineering approach could be used in other areas where the balance between clothing performance and wear comfort is critical, e.g. sport, work wear etc.


Author(s):  
D. Cherns

The use of high resolution electron microscopy (HREM) to determine the atomic structure of grain boundaries and interfaces is a topic of great current interest. Grain boundary structure has been considered for many years as central to an understanding of the mechanical and transport properties of materials. Some more recent attention has focussed on the atomic structures of metalsemiconductor interfaces which are believed to control electrical properties of contacts. The atomic structures of interfaces in semiconductor or metal multilayers is an area of growing interest for understanding the unusual electrical or mechanical properties which these new materials possess. However, although the point-to-point resolutions of currently available HREMs, ∼2-3Å, appear sufficient to solve many of these problems, few atomic models of grain boundaries and interfaces have been derived. Moreover, with a new generation of 300-400kV instruments promising resolutions in the 1.6-2.0 Å range, and resolutions better than 1.5Å expected from specialist instruments, it is an appropriate time to consider the usefulness of HREM for interface studies.


Author(s):  
Jorge Perdigao

In 1955, Buonocore introduced the etching of enamel with phosphoric acid. Bonding to enamel was created by mechanical interlocking of resin tags with enamel prisms. Enamel is an inert tissue whose main component is hydroxyapatite (98% by weight). Conversely, dentin is a wet living tissue crossed by tubules containing cellular extensions of the dental pulp. Dentin consists of 18% of organic material, primarily collagen. Several generations of dentin bonding systems (DBS) have been studied in the last 20 years. The dentin bond strengths associated with these DBS have been constantly lower than the enamel bond strengths. Recently, a new generation of DBS has been described. They are applied in three steps: an acid agent on enamel and dentin (total etch technique), two mixed primers and a bonding agent based on a methacrylate resin. They are supposed to bond composite resin to wet dentin through dentin organic component, forming a peculiar blended structure that is part tooth and part resin: the hybrid layer.


Author(s):  
S. J. Krause ◽  
W.W. Adams ◽  
S. Kumar ◽  
T. Reilly ◽  
T. Suziki

Scanning electron microscopy (SEM) of polymers at routine operating voltages of 15 to 25 keV can lead to beam damage and sample image distortion due to charging. Imaging polymer samples with low accelerating voltages (0.1 to 2.0 keV), at or near the “crossover point”, can reduce beam damage, eliminate charging, and improve contrast of surface detail. However, at low voltage, beam brightness is reduced and image resolution is degraded due to chromatic aberration. A new generation of instruments has improved brightness at low voltages, but a typical SEM with a tungsten hairpin filament will have a resolution limit of about 100nm at 1keV. Recently, a new field emission gun (FEG) SEM, the Hitachi S900, was introduced with a reported resolution of 0.8nm at 30keV and 5nm at 1keV. In this research we are reporting the results of imaging coated and uncoated polymer samples at accelerating voltages between 1keV and 30keV in a tungsten hairpin SEM and in the Hitachi S900 FEG SEM.


Author(s):  
Thomas J. Deerinck ◽  
Maryann E. Martone ◽  
Varda Lev-Ram ◽  
David P. L. Green ◽  
Roger Y. Tsien ◽  
...  

The confocal laser scanning microscope has become a powerful tool in the study of the 3-dimensional distribution of proteins and specific nucleic acid sequences in cells and tissues. This is also proving to be true for a new generation of high contrast intermediate voltage electron microscopes (IVEM). Until recently, the number of labeling techniques that could be employed to allow examination of the same sample with both confocal and IVEM was rather limited. One method that can be used to take full advantage of these two technologies is fluorescence photooxidation. Specimens are labeled by a fluorescent dye and viewed with confocal microscopy followed by fluorescence photooxidation of diaminobenzidine (DAB). In this technique, a fluorescent dye is used to photooxidize DAB into an osmiophilic reaction product that can be subsequently visualized with the electron microscope. The precise reaction mechanism by which the photooxidation occurs is not known but evidence suggests that the radiationless transfer of energy from the excited-state dye molecule undergoing the phenomenon of intersystem crossing leads to the formation of reactive oxygen species such as singlet oxygen. It is this reactive oxygen that is likely crucial in the photooxidation of DAB.


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