The potential of metabolomics for Leishmania research in the post-genomics era

SUMMARYThe post-genomics era has provided researchers with access to a new generation of tools for the global characterization and understanding of pathogen diversity. This review provides a critical summary of published Leishmania post-genomic research efforts to date, and discusses the potential impact of the addition of metabolomics to the post-genomic toolbox. Metabolomics aims at understanding biology by comprehensive metabolite profiling. We present an overview of the design and interpretation of metabolomics experiments in the context of Leishmania research. Sample preparation, measurement techniques, and bioinformatics analysis of the generated complex datasets are discussed in detail. To illustrate the concepts and the expected results of metabolomics analyses, we also present an overview of comparative metabolic profiles of drug-sensitive and drug-resistant Leishmania donovani clinical isolates.

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Novel Antibiotics from Marine Sources

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2011.4.3.2 ◽

2011 ◽

Vol 4 (3) ◽

pp. 1446-1461 ◽

Cited By ~ 1

Author(s):

E.A. Martis ◽

G M Doshi ◽

G V Aggarwal ◽

P P Shanbhag

Keyword(s):

Pharmaceutical Industry ◽

Resistant Bacteria ◽

Drug Resistant ◽

Drug Resistant Bacteria ◽

Terrestrial Life ◽

New Antibiotics ◽

Antibiotic Compounds ◽

New Generation ◽

Novel Antibiotics ◽

Untapped Resource

With the emergence of newer diseases, resistant forms of infectious diseases and multi-drug resistant bacteria, it has become essential to develop novel and more effective antibiotics. Current antibiotics are obtained from terrestrial life or made synthetically from intermediates. The ocean represents virtually untapped resource from which novel antibiotic compounds can be discovered. It is the marine world that will provide the pharmaceutical industry with the next generation of antibiotics. Marine antibiotics are antibiotics obtained from marine organisms. Scientists have reported the discovery of various antibiotics from marine bacteria (aplasmomycin, himalomycins, and pelagiomycins), sponges (Ara C, variabillin, strobilin, ircinin-1, aeroplysin, 3,5-dibromo-4-hydroxyphenylacetamide), coelenterates (asperidol and eunicin), mollusks (laurinterol and pachydictyol), tunicates (geranylhydroquinone and cystadytins), algae (cycloeudesmol, aeroplysinin-1(+), prepacifenol and tetrabromoheptanone), worms (tholepin and 3,5-dibromo-4-hydroxybezaldehyde), and actinomycetes (marinomycins C and D). This indicates that the marine environment, representing approximately half of the global diversity, is an enormous resource for new antibiotics and this source needs to be explored for the discovery of new generation antibiotics. The present article provides an overview of various antibiotics obtained from marine sources.

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Metabolic profiles of multidrug resistant and extensively drug resistant Mycobacterium tuberculosis unveiled by metabolomics

Tuberculosis ◽

10.1016/j.tube.2020.102043 ◽

2021 ◽

Vol 126 ◽

pp. 102043

Author(s):

Amanda Mendes Rêgo ◽

Duanne Alves da Silva ◽

Nicole Victor Ferreira ◽

Lucindo Cardoso de Pina ◽

Joseph A.M. Evaristo ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Multidrug Resistant ◽

Metabolic Profiles ◽

Drug Resistant ◽

Extensively Drug Resistant

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XD™ Titanium Aluminide Composites

MRS Proceedings ◽

10.1557/proc-120-29 ◽

1988 ◽

Vol 120 ◽

Cited By ~ 14

Author(s):

L. Christodoulou ◽

P. A. Parrish ◽

C. R. Crowe

Keyword(s):

Titanium Aluminide ◽

Elevated Temperatures ◽

Coefficient Of Thermal Expansion ◽

Measurement Techniques ◽

Physical Property ◽

Casting Process ◽

Matrix Composition ◽

Al Alloy ◽

Matrix Composites ◽

New Generation

AbstractThe advantages of reinforcing metals with ceramic particles to produce metal matrix composites are well known. The behavior of discontinuously reinforced intermetallic compounds, however, has not been extensively studied. Martin Marietta Laboratories has produced a new generation of discontinuously reinforced titanium aluminide composites using a proprietary casting process known as XD™ technology. These new materials possess enhanced properties at room and elevated temperatures and may be cast, extruded, or forged. The effects of matrix composition, reinforcing phase, and thermal mechanical processing on properties have been studied using optical and various electron microscopy and mechanical and physical property measurement techniques to characterize the alloys. To date, most work has been done on a two-phased lamellar Ti-45 a/o Al alloy reinforced with TiB2 ceramic having an equiaxed morphology. Data on temperature dependence of the dynamic Young's modulus, coefficient of thermal expansion, deformation and fracture behavior, and microstructure are presented.

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7,8-dihydroxyflavone-functionalized gold nanoparticles target the arginase enzyme of Leishmania donovani

Nanomedicine ◽

10.2217/nnm-2021-0161 ◽

2021 ◽

Author(s):

Pragya Prasanna ◽

Prakash Kumar ◽

Saptarshi Mandal ◽

Tanvi Payal ◽

Saurabh Kumar ◽

...

Keyword(s):

Gold Nanoparticles ◽

Leishmania Donovani ◽

Drug Resistant ◽

X Ray Diffraction ◽

High Biological Activity ◽

Transmission Electron ◽

Resistant Strains ◽

Low Cytotoxicity ◽

Drug Resistant Strains ◽

Parasite Leishmania

Aim: To analyze the efficacy and possible mechanism of action of 7,8-dihydroxyflavone (DHF) and DHF synthesized gold nanoparticles (GNPs) against the parasite Leishmania donovani. Methods: GNPs were synthesized using DHF and characterized by dynamic light scattering, ζ potential, Fourier transform infrared spectroscopy, transmission electron microscopy and x-ray diffraction. The efficacy of DHF and DHF-GNP were tested against sensitive and drug-resistant parasites. GNP uptake was measured on macrophages by atomic absorption spectroscopy. Results: DHF and DHF-GNP (∼35 nm) were equally effective against sensitive and drug-resistant strains and inhibited the arginase activity of parasites. Increased IFN-γ and reduced IL-12 cytokine response showed a Th1/Th2-mediated cell death in macrophages. Conclusion: The low cytotoxicity and high biological activity of DHF-GNP may be useful for chemotherapy of leishmaniasis.

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The Potential Impact of Up-Front Drug Sensitivity Testing on India’s Epidemic of Multi-Drug Resistant Tuberculosis

PLoS ONE ◽

10.1371/journal.pone.0131438 ◽

2015 ◽

Vol 10 (7) ◽

pp. e0131438 ◽

Cited By ~ 21

Author(s):

Kuldeep Singh Sachdeva ◽

Neeraj Raizada ◽

Radhey Shyam Gupta ◽

Sreenivas Achuthan Nair ◽

Claudia Denkinger ◽

...

Keyword(s):

Drug Sensitivity ◽

Drug Resistant ◽

Sensitivity Testing ◽

Drug Resistant Tuberculosis ◽

Drug Sensitivity Testing ◽

Resistant Tuberculosis ◽

Multi Drug Resistant Tuberculosis ◽

Potential Impact

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Potential Impact of New Generation Immunotherapy on Years of Life Lost and Lifetime Earnings in Metastatic Melanoma

Annals of Oncology ◽

10.1093/annonc/mdu341.8 ◽

2014 ◽

Vol 25 ◽

pp. iv359

Author(s):

K. Kulig ◽

C. De Moor ◽

B. Korytowsky ◽

M. Halperin ◽

M. Danese

Keyword(s):

Metastatic Melanoma ◽

Years Of Life Lost ◽

Potential Impact ◽

Lifetime Earnings ◽

New Generation

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Extensive Metabolic Profiles of Leaves and Stems from the Medicinal Plant Dendrobium officinale Kimura et Migo

Metabolites ◽

10.3390/metabo9100215 ◽

2019 ◽

Vol 9 (10) ◽

pp. 215 ◽

Cited By ~ 9

Author(s):

Cao ◽

Ji ◽

Li ◽

Lu ◽

Tian ◽

...

Keyword(s):

Genomic Research ◽

Dendrobium Officinale ◽

Metabolic Profiles ◽

Asian Countries ◽

Targeted Metabolomics ◽

Medicinal Species ◽

Metabolomic Data ◽

Metabolite Profiles ◽

Widely Targeted Metabolomics ◽

Metabolic Data

Dendrobium officinale Kimura et Migo is a commercially and pharmacologically highly prized species widely used in Western Asian countries. In contrast to the extensive genomic and transcriptomic resources generated in this medicinal species, detailed metabolomic data are still missing. Herein, using the widely targeted metabolomics approach, we detect 649 diverse metabolites in leaf and stem samples of D. officinale. The majority of these metabolites were organic acids, amino acids and their derivatives, nucleotides and their derivatives, and flavones. Though both organs contain similar metabolites, the metabolite profiles were quantitatively different. Stems, the organs preferentially exploited for herbal medicine, contained larger concentrations of many more metabolites than leaves. However, leaves contained higher levels of polyphenols and lipids. Overall, this study reports extensive metabolic data from leaves and stems of D. officinale, providing useful information that supports ongoing genomic research and discovery of bioactive compounds.

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Comprehensive detection of recurring genomic abnormalities: a targeted sequencing approach for multiple myeloma

Blood Cancer Journal ◽

10.1038/s41408-019-0264-y ◽

2019 ◽

Vol 9 (12) ◽

Cited By ~ 11

Author(s):

Venkata Yellapantula ◽

Malin Hultcrantz ◽

Even H. Rustad ◽

Ester Wasserman ◽

Dory Londono ◽

...

Keyword(s):

Multiple Myeloma ◽

Clinical Trial Design ◽

High Sensitivity ◽

Genomic Research ◽

Molecular Diagnostic ◽

Diagnostic Tools ◽

Genomic Markers ◽

Next Generation Sequencing Ngs ◽

New Generation ◽

Gains And Losses

AbstractRecent genomic research efforts in multiple myeloma have revealed clinically relevant molecular subgroups beyond conventional cytogenetic classifications. Implementing these advances in clinical trial design and in routine patient care requires a new generation of molecular diagnostic tools. Here, we present a custom capture next-generation sequencing (NGS) panel designed to identify rearrangements involving the IGH locus, arm level, and focal copy number aberrations, as well as frequently mutated genes in multiple myeloma in a single assay. We sequenced 154 patients with plasma cell disorders and performed a head-to-head comparison with the results from conventional clinical assays, i.e., fluorescent in situ hybridization (FISH) and single-nucleotide polymorphism (SNP) microarray. Our custom capture NGS panel had high sensitivity (>99%) and specificity (>99%) for detection of IGH translocations and relevant chromosomal gains and losses in multiple myeloma. In addition, the assay was able to capture novel genomic markers associated with poor outcome such as bi-allelic events involving TP53. In summary, we show that a multiple myeloma designed custom capture NGS panel can detect IGH translocations and CNAs with very high concordance in relation to FISH and SNP microarrays and importantly captures the most relevant and recurrent somatic mutations in multiple myeloma rendering this approach highly suitable for clinical application in the modern era.

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The potential impact of new generation transgenic methods on creating rabbit models of cardiac diseases

Progress in Biophysics and Molecular Biology ◽

10.1016/j.pbiomolbio.2016.05.007 ◽

2016 ◽

Vol 121 (2) ◽

pp. 123-130 ◽

Cited By ~ 12

Author(s):

Z. Bősze ◽

P. Major ◽

I. Baczkó ◽

K.E. Odening ◽

L. Bodrogi ◽

...

Keyword(s):

Cardiac Diseases ◽

Potential Impact ◽

New Generation

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Prospects for the use of new generation of anti-tuberculosis drugs in treatment of drug-resistant tuberculosis

Reviews on Clinical Pharmacology and Drug Therapy ◽

10.17816/rcf182115-121 ◽

2020 ◽

Vol 18 (2) ◽

pp. 115-121

Author(s):

Mariia V. Pavlova ◽

Tatiana I. Vinogradova ◽

Natalia V. Zabolotnykh ◽

Elena S. Ershova ◽

Nadezhda V. Sapozhnikova ◽

...

Keyword(s):

Multidrug Resistant ◽

Multi Drug Resistance ◽

Drug Resistant ◽

Control Groups ◽

Drug Resistant Tuberculosis ◽

X Ray ◽

Treatment Regimens ◽

Resistant Tuberculosis ◽

New Generation ◽

Inflammatory Changes

The aim of this work was an experimental and clinical study of increasing of the efficiency of therapy of respiratory tuberculosis with drug resistant pathogen by including a combination of anti-tuberculosis drugs of new generation Bedaquiline (Bq) and Thioureidoiminomethylpyridi-nium perchlorate (perchlozone, Tpp) into treatment regimens. The presented results were obtained: in the experiment on a model of tuberculosis infection in 103 male C57black / 6 mice, reproduced by inserting into the lateral vein of the tail of a suspense of clinical strains of Mycobacterium tuberculosis with multi-drug resistance (MDR) and with various combinations of mutations in the genes; in the clinic on the basis of examination and treatment of 148 patients with multidrug-resistant respiratory tuberculosis. It is shown that the application of the therapy schemes for multiple and broad drug resistant tuberculosis, containing Bq and Tpp in combination with drugs, selected taking into account the drug sensitivity of mycobacteria, allows to significantly reduce the periods of relief of symptoms of intoxication, regression of inflammatory changes, abacillation and achieving positive X-ray dynamics. Undesirable phenomena against the background of combined prescription of new generation drugs, as a rule, corresponded to light and moderate severity, and in frequency of development and expression did not differ from control groups of observation.

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