Characterization and prediction of viral loads of Hepatitis B serum samples by using surface-enhance Raman spectroscopy (SERS)

Abstract Background and aim Mac-2-binding protein glycosylation isomer (M2BPGi) is a novel serum diagnostic marker for liver fibrosis in various liver diseases. We aimed to evaluate its role in assessment of liver fibrosis in chronic hepatitis B infection (CHB) with reference to liver stiffness measurement (LSM) by transient elastography (Fibroscan). Design and Methods A case control study. 50 CHB patients with LSM by transient elastography technology and retrievable serum samples and 20 normal volunteers as a control group were recruited. Results 50 CHB patients (M: F = 30:20; mean age 43years ± 10.58) and 20 normal control volunteers (M: F = 12:8; mean age 37years ± 14.5) were recruited. The mean M2BPGi values for control group, F0-F1, F2, F3 and F4 progressively increased with more advanced stages of liver fibrosis: 0.282, 0.719, 1.322, 1.65 and 1.904 COI, respectively (p < 0.001). M2BPGi levels correlated well with liver stiffness (r = 0.911) and moderately with FIB-4 (r = 0.682), and with APRI (r = 0.536) (all p < 0.001). Using cut-off values of 0.455, 1.02, 1.16, 1.66 and 1.71COI for control, F0-F1, F2, F3 and F4 groups, respectively, the AUROCs were 0.996, 0.996, 0.691, 0.794 and 1.00 for control, F0-F1, F2, F3 and F4 groups, respectively. There was a statistically significant but with weak positive correlation between M2BPGi serum level and INR (r = 0.333, p = 0.018). And there was a statistically significant but with weak negative correlation between M2BPGi serum level and platelet count (r = -0.41, p = 0.003) and HBV DNA (r = -0.373, p = 0.008).There was a statistically significance between M2BPGi serum level and the history of varices (p = 0.023) Conclusions WFA+-M2BP is an accurate serum indicator for assessing different stages of liver fibrosis. WFA+-M2BP provides a simple and reliable alternative or complementary method to liver biopsy and FibroScan.

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Classification of Gan Dan Shi Re Pattern and Gan Shen Yin Xu Pattern in Patients with Hepatitis B Cirrhosis Using Metabonomics

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/2697468 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Chao-qun Zhao ◽

Long Chen ◽

Hong Cai ◽

Wei-li Yao ◽

Qun Zhou ◽

...

Keyword(s):

Hepatitis B ◽

Metabolic Pathways ◽

Serum Samples ◽

Flight Mass Spectrometry ◽

Material Basis ◽

Data Points ◽

Scientific Nature ◽

Objective Basis ◽

Important Objective

Objective. This study aimed to analyze the differential metabolites and their metabolic pathways from the serum of patients with hepatitis B cirrhosis, with two typical patterns of Gan Dan Shi Re (GDSR) and Gan Shen Yin Xu (GSYX) based on the theory of traditional Chinese medicine (TCM). It also investigated the variation in the internal material basis for the two types of patterns and provided an objective basis for classifying TCM patterns using metabolomic techniques. Methods. The serum samples taken from 111 qualified patients (40 GDSR cases, 41 GSYX cases, and 30 Latent Pattern (LP) cases with no obvious pattern characters) and 60 healthy volunteers were tested to identify the differential substances relevant to hepatitis B cirrhosis and the two typical TCM patterns under the gas chromatography–time-of-flight mass spectrometry platform. The relevant metabolic pathways of differential substances were analyzed using multidimensional statistical analysis. Results. After excluding the influence of LP groups, six common substances were found in GDSR and GSYX patterns, which were mainly involved in the metabolic pathways of glycine, serine, threonine, and phenylalanine. Eight specific metabolites involved in the metabolic pathways of linoleic, glycine, threonine, and serine existed in the two patterns. Conclusions. The data points on the metabolic spectrum were found to be well distributed among the differential substances between the two typical TCM patterns of patients with hepatitis B cirrhosis using metabolomic techniques. The differential expression of these substances between GDSR and GSYX patterns provided an important objective basis for the scientific nature of TCM pattern classification at the metabolic level.

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Evaluation of Anti-HBs Antibody Immune Response against Hepatitis B virus in Vaccinated People in а North-eastern Bulgaria Region

Folia Medica ◽

10.3897/folmed.61.e47760 ◽

2019 ◽

Vol 61 (4) ◽

pp. 572-578

Author(s):

Denitsa T. Tsaneva-Damyanova ◽

Liliya I. Ivanova ◽

Silviya N. Pavlova ◽

Svetlana B. Todorova ◽

Tsvetelina K. Popova

Keyword(s):

Immune Response ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Age Groups ◽

University Hospital ◽

Human Pathogens ◽

Serum Samples ◽

Post Vaccination ◽

B Virus ◽

Mass Immunization

Introduction: Hepatitis B virus (HBV) is one of the most significant human pathogens responsible for a huge number of acute and chronic liver infectious diseases worldwide. Aim: To find the duration of post-vaccination immune response in individuals allocated to five age groups from 6 months to 20 years. Materials and methods: All tested subjects were born between 1999 and 2018 and therefore covered by the compulsory vaccination program against hepatitis B. For the serological marker anti-HBs Ab we investigated 449 serum samples taken from ambulatory people and patients of St Marina University Hospital in Varna. Results: A positive antibody response (anti-HBs Ab > 10 mIU/ml) was reported in 79.7% (n = 51) of the group of subjects up to one year old, in 70.0% (n = 196) of the subjects in the age range 1 year/1 month to 15 years, and in 39.3% (n = 33) of the subjects 15 years/1 month to 20 years old. Female sex had a better post-vaccination response than male sex with statistically significant relationship between sex and anti-HBs Ab titer (χ2 = 24.76, p <0.01). Conclusions: Regardless of the mass immunization against HBV in Bulgaria, the relative share of chronic HBV infections does not show a downward trend. Therefore, it is very important to study the duration of the post-vaccination immune response by demonstrating the anti-HBs antibodies and to apply a booster dose from the vaccine if needed.

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Epidemiologic profile of viral hepatitis B and C in North of Iran: Results from PERSIAN Guilan Cohort Study (PGCS)

10.21203/rs.3.rs-43560/v2 ◽

2020 ◽

Author(s):

Fariborz Mansour-Ghanaei ◽

Farahnaz Joukar ◽

Mohammadreza Naghipour ◽

Soheil Hassanipour ◽

Sara Yeganeh ◽

...

Keyword(s):

Cohort Study ◽

Hepatitis B ◽

Urine Analysis ◽

Health Impact ◽

Serum Samples ◽

Hcv Prevalence ◽

Viral Hepatitis B ◽

Epidemiologic Profile ◽

North Of Iran ◽

Hcv Genotyping

Abstract Objective: Hepatitis B (HBV) and C (HCV) viruses are two severe infectious diseases with a high global health impact. This study aimed to evaluate the prevalence of HBV and HCV in the Prospective Epidemiological Research Studies of the Iranian Adults (PERSIAN) Guilan Cohort Study through immunological and molecular methods. Results: The blood samples were obtained from 10520 enrolled participants. Complete biochemical and hematological assessments plus urine analysis were done. The presence of HBsAg, anti-HBs, anti-HBc, and anti-HCV antibodies for all participants and HBeAg and anti-HBe antibody for HBV positive patients were evaluated. HBV genomic DNA and HCV genomic RNA were extracted from positive serum samples. The real-time PCR assay was done to quantify HBV and HCV genomes. HCV genotyping was also performed. The HBV and HCV prevalence was 0.24% (95% CI, 0.16 % to 0.35%) and 0.11% (95% CI, 0.06 % to 0.19%), respectively. Rural participants were significantly more HBV positive than urban peoples (P=0.045) while male individuals were significantly more HCV positive than female participants (P=0.013). Our detected HBV and HCV prevalence were lower than other cities/provinces of Iran, which may be due to lifestyle or other unknown reasons.

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Comparison of hepatitis B viral loads and viral antigen levels in child-bearing age women with and without pregnancy

BMC Pregnancy and Childbirth ◽

10.1186/s12884-018-1932-9 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 2

Author(s):

Chenyu Xu ◽

Jingli Liu ◽

Lanhua Liu ◽

Yongchun Bi ◽

Biyun Xu ◽

...

Keyword(s):

Hepatitis B ◽

Viral Antigen ◽

Viral Loads ◽

Child Bearing

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Sensitivity and specificity of commercially available rapid diagnostic tests for viral hepatitis B and C screening in serum samples

PLoS ONE ◽

10.1371/journal.pone.0235036 ◽

2020 ◽

Vol 15 (7) ◽

pp. e0235036

Author(s):

Ganbolor Jargalsaikhan ◽

Miriam Eichner ◽

Delgerbat Boldbaatar ◽

Purevjargal Bat-Ulzii ◽

Oyungerel Lkhagva-Ochir ◽

...

Keyword(s):

Hepatitis B ◽

Viral Hepatitis ◽

Sensitivity And Specificity ◽

Diagnostic Tests ◽

Rapid Diagnostic Tests ◽

Serum Samples ◽

Viral Hepatitis B

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Impaired Humoral Response to Vaccines among HIV-Exposed Uninfected Infants

Clinical and Vaccine Immunology ◽

10.1128/cvi.05065-11 ◽

2011 ◽

Vol 18 (9) ◽

pp. 1406-1409 ◽

Cited By ~ 44

Author(s):

Beatriz Mariana Abramczuk ◽

Taís Nitsch Mazzola ◽

Yara Maria Franco Moreno ◽

Tatiane Queiroz Zorzeto ◽

Wagner Quintilio ◽

...

Keyword(s):

Hepatitis B ◽

Immune Responses ◽

Neutralizing Antibodies ◽

Geometric Mean ◽

Humoral Response ◽

Vaccine Response ◽

Serum Samples ◽

Hiv Exposed ◽

Exposed Uninfected

ABSTRACTLittle is known about the vaccine protective response for infants born from HIV-infected mothers. We evaluated the antibody response to hepatitis B, tetanus, and diphtheria vaccine in vertically HIV-exposed uninfected infants and compared them to those of control infants not exposed to the virus. The quantitative determination of specific neutralizing antibodies against hepatitis B, diphtheria, and tetanus were performed blindly on serum samples. The results showed that 6.7% of the HIV-exposed uninfected individuals were nonresponders to hepatitis B vaccine (anti-HBs titer, <10 mIU/ml), and 64.4% were very good responders (anti-HBs titer, ≥1,000 mIU/ml), whereas only 3.6% of the nonexposed infants were nonresponders (χ2=10.93; 1 df). The HIV-exposed uninfected infants showed protective titers for diphtheria and tetanus but lower geometric mean anti-tetanus titers compared to those of the HIV-unexposed infants. Our data point to the necessity of evaluating vaccine immune responses in these children and reinforced that alterations in lymphocyte numbers and functions reported for newborns from HIV-infected mothers interfere with the vaccine response.

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