scholarly journals Neuroprotection by solanesol against ethidium bromide-induced multiple sclerosis-like neurobehavioral, molecular, and neurochemical alterations in experimental rats

2021 ◽  
Vol 1 (4) ◽  
pp. 100051
Author(s):  
Nidhi Sharma ◽  
Shubham Upadhayay ◽  
Ambika Shandilya ◽  
Rakesh Sahu ◽  
Anshuman Singh ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Ethidium Bromide

scholarly journals Ozone therapy in ethidium bromide-induced demyelination in rats: possible protective effect [abstract]

2019 ◽  
Vol 3 (4) ◽  
Author(s):  
Mohga Samy
Keyword(s):  
Multiple Sclerosis ◽  
Protective Effect ◽  
Ethidium Bromide ◽  
Demyelinating Diseases ◽  
Immune Reactivity ◽  
Methyl Prednisolone ◽  
The Central Nervous System ◽  
Group 5 ◽  
Group 2 ◽  
Oxygen Groups

BACKGROUND: Multiple sclerosis is an autoimmune inflammatory disease of the central nervous system and it is characterized by excessive demyelination PURPOSE: The study aim to investigate the possible protective effect of ozone (O3) in ethidium bromide (EB) induced demyelination in rats either alone or in combination with corticosteroid in order to decreases the dose of steroid therapy. MATERIAL and METHODS: Rats were divided into 7 groups Group (1) normal control rats received saline. Group (2) sham-operated rats received saline. Group (3) sham operated rats received oxygen. Group (4) EB-treated rats received EB. Group (5) EB treated rats received oxygen. Group (6) EB treated rats received methyl prednisolone (MP) Group (7) EB treated rats received half the dose of MP concomitant with ozone. RESULTS: Significant improvement in the brain serotonin, dopamine, noradrenalin. A reduction of MDA,TNF-COX2 immune-reactivity was noticed in MP and oxygen groups . Furthermore, best amelioration was achieved by combining half the dose of methyl-prednisolone with ozone. CONCLUSION: We concluded that ozone has a protective effect on demyelination and can be used due to its protective effect in demyelinating diseases such as multiple sclerosis.

scholarly journals In-vivo Assessment of Neurotoxicological Effect of Mugwort Leaves on Induced Rat Model of Multiple Sclerosis

2020 ◽  
Vol 11 (2) ◽  
pp. 2030-2037
Author(s):  
Neelamma G ◽  
Shaik Harun Rasheed ◽  
Kausar Sulthana ◽  
Nagarani B ◽  
Srilekha P
Keyword(s):  
Multiple Sclerosis ◽  
Ethidium Bromide ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Conduction Block ◽  
Oral Route ◽  
Neuroprotective Activity ◽  
Artemisia Vulgaris ◽  
Biochemical Studies

Multiple sclerosis (MS) is a demyelinating ailment in which the loss of myelin destructs conduction along the affected axons typically resulting in the conduction block. The motive of this protocol is to carry out the neuroprotective activity of Artemisia vulgaris (Mugwort) by induced demyelination of ethidium Bromide in wistar rats. The leaf methanolic extract was treated at 100mg/kg and 200mg/kg weight administered through oral route and continued for 28 days in demyelinated rats. Demyelination was affected by administering intracranial injection of toxin (ethidium Bromide) at the dose of 1µg/0.03ml of PBS per kg body weight. The potency of the extract was analysed in the terms of their behavioural study on the first, second and fourth week. The animals were immolate after 28 days and subjected to histopathological assessment. The unearthing from behavioural histopathological and biochemical studies evince that the methanol extract of Artemisia vulgaris have potential protective effect on the ethidium bromide induced demyelinated rats.

scholarly journals Effects of aquatic exercises in a rat model of brainstem demyelination with ethidium bromide on the beam walking test

2009 ◽  
Vol 67 (3a) ◽  
pp. 652-656 ◽  
Author(s):  
Cíntia Cristina Souza Nassar ◽  
Eduardo Fernandes Bondan ◽  
Sandra Regina Alouche
Keyword(s):  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Experimental Model ◽  
Ethidium Bromide ◽  
Motor Performance ◽  
Demyelinating Disease ◽  
Control Group ◽  
Walking Test ◽  
The Central Nervous System ◽  
The Impact

Multiple sclerosis is a demyelinating disease of the central nervous system associated with varied levels of disability. The impact of early physiotherapeutic interventions in the disease progression is unknown. We used an experimental model of demyelination with the gliotoxic agent ethidium bromide and early aquatic exercises to evaluate the motor performance of the animals. We quantified the number of footsteps and errors during the beam walking test. The demyelinated animals walked fewer steps with a greater number of errors than the control group. The demyelinated animals that performed aquatic exercises presented a better motor performance than those that did not exercise. Therefore aquatic exercising was beneficial to the motor performance of rats in this experimental model of demyelination.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

Deformation of Yeast Plasma Membrane by Ethidium Bromide

1988 ◽  
Vol 46 ◽  
pp. 254-255
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Thin Section ◽  
Ethidium Bromide ◽  
Freeze Fracture ◽  
Mutagenic Effect ◽  
Yeast Cells ◽  
Hydrophobic Region ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Deep Pits

The mutagenic effect of ethidium bromide on the mitochondrial DNA is well established. Using thin section electron microscopy, it was shown that when yeast cells were grown in the presence of ethidium bromide, besides alterations in the mitochondria, the plasma membrane also showed alterations consisting of 75 to 110 nm-deep pits. Furthermore, ethidium bromide induced an increase in the length and number of endoplasmic reticulum and in the number of intracytoplasmic vesicles.Freeze-fracture, by splitting the hydrophobic region of the membrane, allows the visualization of the surface view of the membrane, and consequently, any alteration induced by ethidium bromide on the membrane can be better examined by this method than by the thin section method.Yeast cells, Candida utilis. were grown in the presence of 35 μM ethidium bromide. Cells were harvested and freeze-fractured according to the procedure previously described.

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