FvZFP1 confers transgenic Nicotiana benthamiana resistance against plant pathogens and improves tolerance to abiotic stresses

Plant Science ◽  
2022 ◽  
pp. 111176
Author(s):  
Penghuan Rui ◽  
Xianchu Yang ◽  
Shiqiang Xu ◽  
Zhanqi Wang ◽  
Xueping Zhou ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Nicotiana Benthamiana ◽  
Plant Pathogens

scholarly journals Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
A Maqbool ◽  
H Saitoh ◽  
M Franceschetti ◽  
CEM Stevenson ◽  
A Uemura ◽  
...  
Keyword(s):  
Nicotiana Benthamiana ◽  
Rice Blast ◽  
Plant Pathogens ◽  
Rice Cultivar ◽  
Blast Disease ◽  
Structural Basis ◽  
Immune Receptor ◽  
Immune Receptors ◽  
Direct Recognition

Plants have evolved intracellular immune receptors to detect pathogen proteins known as effectors. How these immune receptors detect effectors remains poorly understood. Here we describe the structural basis for direct recognition of AVR-Pik, an effector from the rice blast pathogen, by the rice intracellular NLR immune receptor Pik. AVR-PikD binds a dimer of the Pikp-1 HMA integrated domain with nanomolar affinity. The crystal structure of the Pikp-HMA/AVR-PikD complex enabled design of mutations to alter protein interaction in yeast and in vitro, and perturb effector-mediated response both in a rice cultivar containing Pikp and upon expression of AVR-PikD and Pikp in the model plant Nicotiana benthamiana. These data reveal the molecular details of a recognition event, mediated by a novel integrated domain in an NLR, which initiates a plant immune response and resistance to rice blast disease. Such studies underpin novel opportunities for engineering disease resistance to plant pathogens in staple food crops.

scholarly journals Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents

2019 ◽  
Vol 7 (8) ◽  
pp. 249 ◽  
Author(s):  
Houda Ben Slama ◽  
Mohamed Ali Triki ◽  
Ali Chenari Bouket ◽  
Fedia Ben Mefteh ◽  
Faizah N. Alenezi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Abiotic Stresses ◽  
Plant Pathogens ◽  
Metal Removal ◽  
Plant Protection ◽  
Biocontrol Agents ◽  
In Planta ◽  
Olive Trees ◽  
Limoniastrum Monopetalum ◽  
Selection Of

Halophyte Limoniastrum monopetalum, an evergreen shrub inhabiting the Mediterranean region, has well-documented phytoremediation potential for metal removal from polluted sites. It is also considered to be a medicinal halophyte with potent activity against plant pathogens. Therefore, L. monopetalum may be a suitable candidate for isolating endophytic microbiota members that provide plant growth promotion (PGP) and resistance to abiotic stresses. Selected for biocontrol abilities, these endophytes may represent multifaceted and versatile biocontrol agents, combining pathogen biocontrol in addition to PGP and plant protection against abiotic stresses. In this study 117 root culturable bacterial endophytes, including Gram-positive (Bacillus and Brevibacillus), Gram-negative (Proteus, Providencia, Serratia, Pantoea, Klebsiella, Enterobacter and Pectobacterium) and actinomycete Nocardiopsis genera have been recovered from L. monopetalum. The collection exhibited high levels of biocontrol abilities against bacterial (Agrobacterium tumefaciens MAT2 and Pectobacterium carotovorum MAT3) and fungal (Alternaria alternata XSZJY-1, Rhizoctonia bataticola MAT1 and Fusarium oxysporum f. sp. radicis lycopersici FORL) pathogens. Several bacteria also showed PGP capacity and resistance to antibiotics and metals. A highly promising candidate Bacillus licheniformis LMRE 36 with high PGP, biocontrol, metal and antibiotic, resistance was subsequently tested in planta (potato and olive trees) for biocontrol of a collection of 14 highly damaging Fusarium species. LMRE 36 proved very effective against the collection in both species and against an emerging Fusarium sp. threatening olive trees culture in nurseries. These findings provide a demonstration of our pyramiding strategy. Our strategy was effective in combining desirable traits in biocontrol agents towards broad-spectrum resistance against pathogens and protection of crops from abiotic stresses. Stacking multiple desirable traits into a single biocontrol agent is achieved by first, careful selection of a host for endophytic microbiota recovery; second, stringent in vitro selection of candidates from the collection; and third, application of the selected biocontrol agents in planta experiments. That pyramiding strategy could be successfully used to mitigate effects of diverse biotic and abiotic stresses on plant growth and productivity. It is anticipated that the strategy will provide a new generation of biocontrol agents by targeting the microbiota of plants in hostile environments.

scholarly journals Conserved fungal effector suppresses PAMP-triggered immunity by targeting plant immune kinases

2018 ◽  
Vol 116 (2) ◽  
pp. 496-505 ◽  
Author(s):  
Hiroki Irieda ◽  
Yoshihiro Inoue ◽  
Masashi Mori ◽  
Kohji Yamada ◽  
Yuu Oshikawa ◽  
...  
Keyword(s):  
Oxidative Burst ◽  
Nicotiana Benthamiana ◽  
Host Plants ◽  
Plant Pathogens ◽  
Defense Responses ◽  
Pathogen Virulence ◽  
The Core ◽  
Bacterial Effectors ◽  
Multiple Pathogens ◽  
Core Effectors

Plant pathogens have optimized their own effector sets to adapt to their hosts. However, certain effectors, regarded as core effectors, are conserved among various pathogens, and may therefore play an important and common role in pathogen virulence. We report here that the widely distributed fungal effector NIS1 targets host immune components that transmit signaling from pattern recognition receptors (PRRs) in plants. NIS1 from two Colletotrichum spp. suppressed the hypersensitive response and oxidative burst, both of which are induced by pathogen-derived molecules, in Nicotiana benthamiana. Magnaporthe oryzae NIS1 also suppressed the two defense responses, although this pathogen likely acquired the NIS1 gene via horizontal transfer from Basidiomycota. Interestingly, the root endophyte Colletotrichum tofieldiae also possesses a NIS1 homolog that can suppress the oxidative burst in N. benthamiana. We show that NIS1 of multiple pathogens commonly interacts with the PRR-associated kinases BAK1 and BIK1, thereby inhibiting their kinase activities and the BIK1-NADPH oxidase interaction. Furthermore, mutations in the NIS1-targeting proteins, i.e., BAK1 and BIK1, in Arabidopsis thaliana also resulted in reduced immunity to Colletotrichum fungi. Finally, M. oryzae lacking NIS1 displayed significantly reduced virulence on rice and barley, its hosts. Our study therefore reveals that a broad range of filamentous fungi maintain and utilize the core effector NIS1 to establish infection in their host plants and perhaps also beneficial interactions, by targeting conserved and central PRR-associated kinases that are also known to be targeted by bacterial effectors.

scholarly journals Impact of Abiotic Stresses on Plant Virus Transmission by Aphids

Viruses ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 216 ◽  
Author(s):  
Manuella van Munster
Keyword(s):  
Climate Change ◽  
Abiotic Stresses ◽  
Plant Pathogens ◽  
Plant Defence ◽  
Virus Transmission ◽  
Plant Viruses ◽  
Research Field ◽  
Viral Transmission ◽  
In Planta ◽  
Elevated Atmospheric Co2

Plants regularly encounter abiotic constraints, and plant response to stress has been a focus of research for decades. Given increasing global temperatures and elevated atmospheric CO2 levels and the occurrence of water stress episodes driven by climate change, plant biochemistry, in particular, plant defence responses, may be altered significantly. Environmental factors also have a wider impact, shaping viral transmission processes that rely on a complex set of interactions between, at least, the pathogen, the vector, and the host plant. This review considers how abiotic stresses influence the transmission and spread of plant viruses by aphid vectors, mainly through changes in host physiology status, and summarizes the latest findings in this research field. The direct effects of climate change and severe weather events that impact the feeding behaviour of insect vectors as well as the major traits (e.g., within-host accumulation, disease severity and transmission) of viral plant pathogens are discussed. Finally, the intrinsic capacity of viruses to react to environmental cues in planta and how this may influence viral transmission efficiency is summarized. The clear interaction between biotic (virus) and abiotic stresses is a risk that must be accounted for when modelling virus epidemiology under scenarios of climate change.

scholarly journals The NLP Toxin Family in Phytophthora sojae Includes Rapidly Evolving Groups That Lack Necrosis-Inducing Activity

2012 ◽  
Vol 25 (7) ◽  
pp. 896-909 ◽  
Author(s):  
Suomeng Dong ◽  
Guanghui Kong ◽  
Dinah Qutob ◽  
Xiaoli Yu ◽  
Junli Tang ◽  
...  
Keyword(s):  
Positive Selection ◽  
Nicotiana Benthamiana ◽  
Plant Pathogens ◽  
Phytophthora Sojae ◽  
The Other ◽  
Death Process ◽  
Birth And Death Process ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Cyst Germination

Necrosis- and ethylene-inducing-like proteins (NLP) are widely distributed in eukaryotic and prokaryotic plant pathogens and are considered to be important virulence factors. We identified, in total, 70 potential Phytophthora sojae NLP genes but 37 were designated as pseudogenes. Sequence alignment of the remaining 33 NLP delineated six groups. Three of these groups include proteins with an intact heptapeptide (Gly-His-Arg-His-Asp-Trp-Glu) motif, which is important for necrosis-inducing activity, whereas the motif is not conserved in the other groups. In total, 19 representative NLP genes were assessed for necrosis-inducing activity by heterologous expression in Nicotiana benthamiana. Surprisingly, only eight genes triggered cell death. The expression of the NLP genes in P. sojae was examined, distinguishing 20 expressed and 13 nonexpressed NLP genes. Real-time reverse-transcriptase polymerase chain reaction results indicate that most NLP are highly expressed during cyst germination and infection stages. Amino acid substitution ratios (Ka/Ks) of 33 NLP sequences from four different P. sojae strains resulted in identification of positive selection sites in a distinct NLP group. Overall, our study indicates that expansion and pseudogenization of the P. sojae NLP family results from an ongoing birth-and-death process, and that varying patterns of expression, necrosis-inducing activity, and positive selection suggest that NLP have diversified in function.

scholarly journals Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ivis Moran-Bertot ◽  
Lianet Rodríguez-Cabrera ◽  
Orlando Borras-Hidalgo ◽  
Siliang Huang ◽  
Yunchao Kan ◽  
...  
Keyword(s):  
Plutella Xylostella ◽  
Nicotiana Benthamiana ◽  
Plant Pathogens ◽  
Constitutive Expression ◽  
Potato Virus X ◽  
Phytophthora Parasitica ◽  
Black Shank ◽  
Pot Trials ◽  
Stromal Cell Derived Factor

Abstract Background The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae. Results The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls. Conclusions We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens.

scholarly journals Fasciclin-like arabinogalactan gene family in Nicotiana benthamiana: genome-wide identification, classification and expression in response to pathogens

2020 ◽  
Author(s):  
Xinyang Wu ◽  
Yuchao Lai ◽  
Lanqing Lv ◽  
Mengfei Ji ◽  
Kelei Han ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Nicotiana Benthamiana ◽  
Plant Pathogens ◽  
Plant Immunity ◽  
Arabinogalactan Proteins ◽  
Cis Acting ◽  
Genome Wide ◽  
A Genome ◽  
Polymerase Chain ◽  
Plant Pathogen Interactions

Abstract Background: Nicotiana benthamiana is widely used as a model plant to study plant-pathogen interactions. Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), participate in mediating plant growth, development and response to abiotic stress. However, the members of FLAs in N. benthamiana and their response to plant pathogens are unknown.Results: 38 NbFLAs were identified from a genome-wide study. NbFLAs could be divided into four subclasses, and their gene structure and motif composition were conserved in each subclass. NbFLAs may be regulated by cis-acting elements such as STRE and MBS, and could possibly be the targets of transcription factors like C2H2. Quantitative real time polymerase chain reaction (RT-qPCR) results showed that selected NbFLAs were differentially expressed in different tissues. All of the selected NbFLAs were significantly downregulated following infection by turnip mosaic virus (TuMV) and most of them also by Pseudomonas syringae pv tomato (Pst) strain DC3000 (Pst DC3000), suggesting possible roles in response to pathogenic infection.Conclusions: This study systematically identified FLAs in N. benthamiana, and indicates their potential roles in response to biotic stress. The identification of NbFLAs will facilitate further studies of their role in plant immunity in N. benthamiana.

scholarly journals Fasciclin-like arabinogalactan gene family in Nicotiana benthamiana: genome-wide identification, classification and expression in response to pathogens

2020 ◽  
Author(s):  
Xinyang Wu ◽  
Yuchao Lai ◽  
Lanqing Lv ◽  
Mengfei Ji ◽  
Kelei Han ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Nicotiana Benthamiana ◽  
Plant Pathogens ◽  
Plant Immunity ◽  
Arabinogalactan Proteins ◽  
Cis Acting ◽  
Genome Wide ◽  
A Genome ◽  
Polymerase Chain ◽  
Plant Pathogen Interactions

Abstract Background: Nicotiana benthamiana is widely used as a model plant to study plant-pathogen interactions. Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), participate in mediating plant growth, development and response to abiotic stress. However, the members of FLAs in N. benthamiana and their response to plant pathogens are unknown.Results: 38 NbFLAs were identified from a genome-wide study. NbFLAs could be divided into four subclasses, and their gene structure and motif composition were conserved in each subclass. NbFLAs may be regulated by cis-acting elements such as STRE and MBS, and may be the targets of transcription factors like C2H2. Quantitative real time polymerase chain reaction (RT-qPCR) results showed that selected NbFLAs were differentially expressed in different tissues. All of the selected NbFLAs were significantly downregulated following infection by turnip mosaic virus (TuMV) and most of them also by Pseudomonas syringae pv tomato strain DC3000 (Pst DC3000), suggesting possible roles in response to pathogenic infection.Conclusions: This study systematically identified FLAs in N. benthamiana, and indicates their potential roles in response to biotic stress. The identification of NbFLAs will facilitate further studies of their role in plant immunity in N. benthamiana.

scholarly journals Transient expression of gene encoding ZmLEA14A protein in Nicotiana benthamiana plant

2020 ◽  
Vol 17 (3) ◽  
pp. 491-497
Author(s):  
Ha Hong Hanh ◽  
Le Thi Thu Hien ◽  
Huynh Thi Thu Hue
Keyword(s):  
Genetic Engineering ◽  
Abiotic Stresses ◽  
Nicotiana Benthamiana ◽  
Lea Proteins ◽  
Lea Protein ◽  
Gene Encoding ◽  
Specific Antibodies ◽  
Vegetative Tissues ◽  
The World ◽  
Infiltration Method

LEA protein family includes proteins accumulated in the late stage of embryogenesis and in vegetative tissues of stress-confronted plant. These proteins have been demontrated to play a major role in plant response to abiotic stresses, such as drought and salinity stress. The genes coding for LEA proteins in maize are divided into 9 groups including LEA 1, LEA 2, LEA 3, LEA 4, LEA 5, LEA 6, SMP, dehydrin, and AtM. The application of LEA genes to improve drought tolerance for plants by genetic engineering has also been studied extensively all over the world. In this study, pCAM/35S-ZmLEA14A-35S vector and pCAM/Ubi-ZmLEA14A-35S vector contained the ZmLEA14A gene isolated from Te vang 1, these vectors were used to transient express into Nicotiana benthamiana tobacco leaves by agro-infiltration method. The results of immunoassay between cmyc specific antibodies with proteins from infected leaves revealed the expression of recombinant ZmLEA14A protein in N. benthamiana leaves. Thereby, two constructs habouring the ZmLEA14A gene work at transcription and translation levels in the model plant that could harnessed for stable transformation in plants.

scholarly journals Interaction between arbuscular mycorrhizal fungi and Bacillus spp. in soil enhancing growth of crop plants

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Anuroopa Nanjundappa ◽  
Davis Joseph Bagyaraj ◽  
Anil Kumar Saxena ◽  
Murugan Kumar ◽  
Hillol Chakdar
Keyword(s):  
Plant Growth ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Abiotic Stresses ◽  
Plant Pathogens ◽  
Arbuscular Mycorrhizal ◽  
Crop Plants ◽  
Symbiotic Association ◽  
Biotic And Abiotic Stresses ◽  
Soil Microbial Population

AbstractSoil microorganisms play an important role in enhancing soil fertility and plant health. Arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria form a key component of the soil microbial population. Arbuscular mycorrhizal fungi form symbiotic association with most of the cultivated crop plants and they help plants in phosphorus nutrition and protecting them against biotic and abiotic stresses. Many species of Bacillus occurring in soil are also known to promote plant growth through phosphate solubilization, phytohormone production and protection against biotic and abiotic stresses. Synergistic interaction between AMF and Bacillus spp. in promoting plant growth compared to single inoculation with either of them has been reported. This is because of enhanced nutrient uptake, protection against plant pathogens and alleviation of abiotic stresses (water, salinity and heavy metal) through dual inoculation compared to inoculation with either AMF or Bacillus alone.

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