Transcriptomic analysis of Entamoeba histolytica reveals domain-specific sense strand expression of LINE-encoded ORFs with massive antisense expression of RT domain

Plasmid ◽  
2021 ◽  
Vol 114 ◽  
pp. 102560
Author(s):  
Devinder Kaur ◽  
Mridula Agrahari ◽  
Shashi Shekhar Singh ◽  
Prabhat Kumar Mandal ◽  
Alok Bhattacharya ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Transcriptomic Analysis ◽  
Antisense Expression ◽  
Domain Specific ◽  
Sense Strand ◽  
Specific Sense

Amounts and modulation of actin mRNAs in mouse oocytes and embryos

Development ◽  
1989 ◽  
Vol 106 (3) ◽  
pp. 561-565 ◽  
Author(s):  
R. Bachvarova ◽  
E.M. Cohen ◽  
V. De Leon ◽  
K. Tokunaga ◽  
S. Sakiyama ◽  
...  
Keyword(s):  
Growth Phase ◽  
Developmental Stages ◽  
Cdna Sequences ◽  
Mouse Oocytes ◽  
Rna Probes ◽  
Sense Strand ◽  
Polysomal Mrna ◽  
Mrna Content ◽  
Actin Mrna ◽  
Specific Sense

In order to measure the content of beta- and gamma-actin mRNA in mouse oocytes and ovulated eggs, Northern and slot blots were hybridized to complementary RNA probes transcribed from mouse isotype-specific cDNA sequences. The blots included samples of isotype-specific sense strand RNA standards prepared from the same cDNA sequences. Total actin mRNA content was estimated to be 40 fg per preovulatory full-grown oocyte or egg, consisting of one-third beta-actin mRNA and two-thirds gamma-actin mRNA. Ninety per cent of the actin mRNA is on polysomes in full-grown oocytes. The per cent of actin mRNA in polysomal mRNA is similar to the per cent of actin in newly synthesized proteins. Measurements on other developmental stages showed that, in mid-growth-phase oocytes, each actin mRNA reaches a level twofold higher than in full-grown oocytes. Thereafter, all modulations of the two isotypic mRNAs occur in parallel; that is, they are maintained at constant levels during the late growth phase (oocytes from females 8–14 days old); gradually degraded in oocytes that have completed their rapid growth phase (oocytes from females 15–18 days old), in maturing oocytes, and in 1- and 2-cell embryos; and deadenylated after about 7 h of progression into meiotic maturation.

scholarly journals Transcriptomic analysis reveals novel downstream regulatory motifs and highly transcribed virulence factor genes of Entamoeba histolytica

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Sarah Naiyer ◽  
Devinder Kaur ◽  
Jamaluddin Ahamad ◽  
Shashi Shekhar Singh ◽  
Yatendra Pratap Singh ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Virulence Factor ◽  
Transcriptomic Analysis ◽  
Regulatory Motifs ◽  
Virulence Factor Genes

scholarly journals Advances in Entamoeba histolytica Biology Through Transcriptomic Analysis

2019 ◽  
Vol 10 ◽  
Author(s):  
Sarah Naiyer ◽  
Alok Bhattacharya ◽  
Sudha Bhattacharya
Keyword(s):  
Entamoeba Histolytica ◽  
Transcriptomic Analysis

scholarly journals Unsupervised Predominant Sense Detection and Its Application to Text Classification

2020 ◽  
Vol 10 (17) ◽  
pp. 6052
Author(s):  
Attaporn Wangpoonsarp ◽  
Kazuya Shimura ◽  
Fumiyo Fukumoto
Keyword(s):  
Text Classification ◽  
Word Sense Disambiguation ◽  
Semantic Context ◽  
Word Sense ◽  
Domain Specific ◽  
Markov Random ◽  
Markov Random Walk ◽  
Sense Disambiguation ◽  
Overall Performance ◽  
Specific Sense

This paper focuses on the domain-specific senses of words and proposes a method for detecting predominant sense depending on each domain. Our Domain-Specific Senses (DSS) model is an unsupervised manner and detects predominant senses in each domain. We apply a simple Markov Random Walk (MRW) model to ranking senses for each domain. It decides the importance of a sense within a graph by using the similarity of senses. The similarity of senses is obtained by using distributional representations of words from gloss texts in the thesaurus. It can capture large semantic context and thus does not require manual annotation of sense-tagged data. We used the Reuters corpus and the WordNet in the experiments. We applied the results of domain-specific senses to text classification and examined how DSS affects the overall performance of the text classification task. We compared our DSS model with one of the word sense disambiguation techniques (WSD), Context2vec, and the results demonstrate our domain-specific sense approach gains 0.053 F1 improvement on average over the WSD approach.

Erythrophagocytosis by Entamoeba histolytica.- Ultrastructural Study

1972 ◽  
Vol 30 ◽  
pp. 156-157 ◽  
Author(s):  
Norberto Treviño ◽  
Alfredo Feria-Velasco ◽  
I. Ruiz de Chávez
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Entamoeba Histolytica ◽  
Ultrastructural Study ◽  
Saline Solution ◽  
Physiological Saline ◽  
Ultrastructural Level ◽  
Hot Plate ◽  
Physiological Saline Solution ◽  
Axenic Conditions

Although erythrophagocytosis by various species of Entamoeba is a well known phenomenon this has not yet been studied in detail at the ultrastructural level. The present work deals with the description of the incorporation process of erythrocytes by trophozoites of E. histolytica. For this study, trophozoites of E. histolytica, HK-9:NIH strain cultured in axenic conditions and washed human erythrocytes were placed on a hot plate at 37°C in physiological saline solution. After 5 minutes, 2.5% glutarldehyde was added and the samples were processed according to conventional techniques for electron microscopy.Based upon light microscopy studies on living trophozoites in contact with erythrocytes, it seems that erythrophagocytosis only takes place in one pole of the parasite.

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

Scientific Problem Solving in a Virtual Laboratory: A Comparison Between Individuals and Pairs

2008 ◽  
Vol 67 (2) ◽  
pp. 71-83 ◽  
Author(s):  
Yolanda A. Métrailler ◽  
Ester Reijnen ◽  
Cornelia Kneser ◽  
Klaus Opwis
Keyword(s):  
Visual Search ◽  
Problem Solving ◽  
Process Data ◽  
Scientific Problem ◽  
Domain Specific ◽  
Scientific Problem Solving ◽  
Before And After ◽  
Verbal Data ◽  
Psychological Knowledge ◽  
Problem Solving Task

This study compared individuals with pairs in a scientific problem-solving task. Participants interacted with a virtual psychological laboratory called Virtue to reason about a visual search theory. To this end, they created hypotheses, designed experiments, and analyzed and interpreted the results of their experiments in order to discover which of five possible factors affected the visual search process. Before and after their interaction with Virtue, participants took a test measuring theoretical and methodological knowledge. In addition, process data reflecting participants’ experimental activities and verbal data were collected. The results showed a significant but equal increase in knowledge for both groups. We found differences between individuals and pairs in the evaluation of hypotheses in the process data, and in descriptive and explanatory statements in the verbal data. Interacting with Virtue helped all students improve their domain-specific and domain-general psychological knowledge.

Emotions and health: The impact of emotions, emotions regulation, music, and acculturation on health

2008 ◽  
Vol 16 (3) ◽  
pp. 112-115 ◽  
Author(s):  
Stephan Bongard ◽  
Volker Hodapp ◽  
Sonja Rohrmann
Keyword(s):  
Stress Responses ◽  
Physiological Stress ◽  
Anger Expression ◽  
Domain Specific ◽  
Cardiovascular Stress ◽  
Expression Behavior ◽  
Cardio Vascular ◽  
And Coping ◽  
Relationship Of ◽  
The Impact

Abstract. Our unit investigates the relationship of emotional processes (experience, expression, and coping), their physiological correlates and possible health outcomes. We study domain specific anger expression behavior and associated cardio-vascular loads and found e.g. that particularly an open anger expression at work is associated with greater blood pressure. Furthermore, we demonstrated that women may be predisposed for the development of certain mental disorders because of their higher disgust sensitivity. We also pointed out that the suppression of negative emotions leads to increased physiological stress responses which results in a higher risk for cardiovascular diseases. We could show that relaxation as well as music activity like singing in a choir causes increases in the local immune parameter immunoglobuline A. Finally, we are investigating connections between migrants’ strategy of acculturation and health and found e.g. elevated cardiovascular stress responses in migrants when they where highly adapted to the German culture.

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