Phenotype characterization of crossed beaks in Beijing-You chickens based on morphological observation

Ficus spp. belongs to the tribe Ficeae in the Moraceae family. Many members of this genus have been collected and grown in Bogor Botanic Gardens. There are 519 living collections of Ficus conserved since 1817, and 13 of them have not been identified until the species level. This research aimed to identify the Ficus sp. originated from Kaur Selatan (Bengkulu) using morphological and molecular approaches. Morphological characterization and herbarium specimen observation have been carried out to identify the Ficus sp. The molecular approach was conducted through DNA barcoding using ITS primer. The molecular identification using ITS sequence showed that Ficus sp. is Ficus crassiramea with 99.87% similarity to the sequence in NCBI. Morphological observation through herbarium specimen showed that there are 9 vegetative characters specific to Ficus crassiramea.

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Morphology and molecular characterization of newly isolated microalgae strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and its potential use

ANNALES BOGORIENSES ◽

10.14203/ann.bogor.2019.v23.n1.13-19 ◽

2019 ◽

Vol 23 (1) ◽

pp. 13

Author(s):

Delicia Yunita Rahman ◽

Swastika Praharyawan ◽

Sapto Raharjo ◽

Farizul Fadiyah ◽

Dwi Susilaningsih

Keyword(s):

Amino Acid ◽

Molecular Characterization ◽

Acid Content ◽

National Park ◽

Amino Acid Content ◽

Morphological Observation ◽

Future Application ◽

Health Application ◽

Potential Use

Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.

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Morphological characterization of gram-positive and gram-negative bacteria from treated latex processing wastewater

Environmental and Toxicology Management ◽

10.33086/etm.v1i2.2263 ◽

2021 ◽

Vol 1 (2) ◽

pp. 32-36

Author(s):

Farrah Aini Dahalan ◽

Nor Azizah Parmin

Keyword(s):

Oxygen Demand ◽

Morphological Characterization ◽

The Other ◽

Morphological Properties ◽

Morphological Observation ◽

Gram Positive ◽

Bacterial Colony ◽

Gram Negative ◽

Gram Staining

A preliminary morphological screening and isolation of bacterial colony from latex industrial wastewater was carried out. Bacteria colonies from latex processing wastewater were isolated from a local latex processing industry. It was found that 17 bacterial isolates had been purified grown on nutrient agar under 35˚C. The colonies were then purified and morphologically indicated via Gram staining and motility test. After morphological observation, it was identified that out of 17 isolates, 9 isolates were Gram positive and 8 isolates were Gram negative. There are 11 out of 17 colonies were rod-shaped bacterial colonies, while the other 6 colonies were cocci-shaped bacteria. There were 11 colonies of gliding bacteria, three colonies were non-motile bacteria and the other three colonies were flagellated bacteria. This study is only limited to morphological observation as the main aim of this study was to investigate the potential occurrence of viable growth in treated latex processing wastewater. The bacterial colonies were classified base on their morphological properties shown. This study has classified several genera such as Staphylococcus, Escherichia, Thiobacillus, Arthrobacter and other Genus. The growth curve of 17 isolates studied and the chemical oxygen demand were determined.

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Corrigendum to “Genotype-Phenotype Characterization of Novel Variants in Six Italian Patients with Familial Exudative Vitreoretinopathy”

Journal of Ophthalmology ◽

10.1155/2017/7969364 ◽

2017 ◽

Vol 2017 ◽

pp. 1-2

Author(s):

Giancarlo Iarossi ◽

Matteo Bertelli ◽

Paolo Enrico Maltese ◽

Elena Gusson ◽

Giorgio Marchini ◽

...

Keyword(s):

Familial Exudative Vitreoretinopathy ◽

Novel Variants ◽

Phenotype Characterization

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Prevalence and Genetic Characterization of Two Mitochondrial Gene Sequences of Strobilocercus Fasciolaris in the Livers of Brown Rats (Rattus norvegicus) in Heilongjiang Province in Northeastern China

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2020.588107 ◽

2020 ◽

Vol 10 ◽

Author(s):

Fengnian Zhao ◽

Yun Zhou ◽

Yanchen Wu ◽

Kexin Zhou ◽

Aiqin Liu ◽

...

Keyword(s):

Amino Acid ◽

Intraspecific Variation ◽

Rattus Norvegicus ◽

Genetic Characterization ◽

Northeastern China ◽

Amino Acid Sequences ◽

Morphological Observation ◽

Heilongjiang Province ◽

Gene Sequences

Rodents constitute the largest and most successful group of mammals worldwide. Brown rats (Rattus norvegicus) are one of the most common rodent species, and they serve as intermediate hosts of Hydatigera taeniaeformis. Although there have been a few studies reporting on the presence of the larval form of H. taeniaeformis (strobilocercus fasciolaris) in brown rats worldwide, little information is available on the genetic characterization of this parasite, with no molecular data from China. Therefore, from April 2014 to March 2016, this study was carried out to understand the prevalence and genetic characters of strobilocercus fasciolaris in brown rats captured in Heilongjiang Province in northeastern China. The livers of brown rats were collected and examined for the presence of cysts. Each cyst was identified based on morphological observation: the larvae with the naked eye and the scolexes under a microscope. The results were confirmed by polymerase chain reaction (PCR) and sequencing of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4) genes. At the investigated sites, 11.8% (13/110) of the brown rats were infected with strobilocercus fasciolaris. Based on sequence analysis, there were 10 and six haplotypes regarding the cox1 and the nad4 loci, with 24 and 42 polymorphic sites, respectively (degree of intraspecific variation: 0.3%–4.4% and 0.6%–4.7%, respectively). Twelve nucleotide sequences (six of the 10 at the cox1 locus and all six at the nad4 locus) have not previously been described. Base differences in three of the six novel cox1 gene sequences and five of the six novel nad4 gene sequences caused amino acid changes. Phylogenetic analyses of the cox1 and nad4 gene sequences based on neighbor-joining and Bayesian inference trees indicated that all the strobilocercus fasciolaris isolates belonged to Hydatigera taeniaeformis sensu stricto (s.s.). This is the first report on the genetic characterization of strobilocercus fasciolaris in brown rats in China. The findings of novel cox1 and nad4 nucleotide and amino acid sequences may reflect the region-specific genetic characterization of the parasite. The data will be useful to explore the biological and epidemiological significance of the intraspecific variation within H. taeniaeformis s.s.

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Synthesis and Characterization of Boron Nitride Nanotubes-Polycaprolactone Nanocomposite

Materials Science Forum ◽

10.4028/www.scientific.net/msf.951.39 ◽

2019 ◽

Vol 951 ◽

pp. 39-44 ◽

Cited By ~ 1

Author(s):

Akesh Babu Kakarla ◽

Cin Kong ◽

Wei Kong ◽

Ing Kong

Keyword(s):

Boron Nitride ◽

Polymer Matrix ◽

Boron Nitride Nanotubes ◽

Annealing Process ◽

Morphological Observation ◽

Mechanical And Thermal Properties ◽

Thermal Curing ◽

Boron Powder ◽

Co Precipitation

This work aimed to investigate the effects of boron nitride nanotubes (BNNTs) on the mechanical and thermal properties of BNNT-polycaprolactone (PCL) nanocomposites. BNNTs were synthesized via co-precipitation and annealing process by using amorphous boron powder as a precursor. PCL-BNNT nanocomposites were prepared by using sonication and thermal curing. Their morphology, thermal and tensile properties were characterized. Morphological observation revealed that BNNTs were arbitrarily and evenly dispersed within the PCL which indicated a good dispersion of the nanotubes in polymer matrix. Both the thermal stability and mechanical properties of neat PCL were enhanced with the addition of BNNTs to polymer matrix, in particular the tensile strength of PLC was increased by 101% with the addition of 5 wt% nanofillers.

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Anodization of Highly Ordered Titania Nanotube Prepared with Organic Electrolyte

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.846.23 ◽

2020 ◽

Vol 846 ◽

pp. 23-28

Author(s):

Bambang Suharno ◽

Nabila Ramadhanti ◽

Nadya Aryani ◽

Ahmad Zakiyuddin ◽

Sugeng Supriadi

Keyword(s):

Bone Cells ◽

Cell Attachment ◽

Morphological Observation ◽

Tube Length ◽

Fluoride Ions ◽

Organic Electrolytes ◽

Duration Time ◽

Cross Section Area ◽

Section Area

Ti-6Al-4V as an implant material has bio-inert properties, so it does not support any tissues or bone cells reaction. This study aims to increase the tendency of osteoblast's cell attachment to the surface of implant Ti-6Al-4V by fabricating nanotube structure on the surface by anodization. This study also conducted to study the effect of elements from titanium alloys and organic electrolytes on the mechanism of formation of nanotube structures. The anodization method was chosen because it was easy to do, effective, and inexpensive. The samples were prepared by ground and polished, then washed by ultrasonic. Anodization used organic electrolytes in the form of a mixture of ethylene glycol, 0.5 M NH4F, and 4 w.t% deionized water. The study of the effect of voltage and duration time was carried out to understand the mechanism of nanotube formation, through morphological observation on the surface and cross-section area of nanotubes using SEM and characterization of elements using EDS, diameter, and length of highly ordered nanotubes was observed. The results of the characterization showed that the tube diameter is adjusted by the voltage, while duration time influence the tube length, with a linear relationship, so the widest diameter achieved at 40V 5h, but the longest tube achieved at 30 V 5h. Whereas for 5h duration, the upper part of the tube collapsed and disintegrated. The fluoride ions incorporated at the tube surfaces formed fluoride-titanium oxide cubic agglomerates, and the whole nanotube surface was oxide.

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