A novel bZIP transcription factor ClrC positively regulates multiple stress responses, conidiation and cellulase expression in Penicillium oxalicum

2016 ◽  
Vol 167 (5) ◽  
pp. 424-435 ◽  
Author(s):  
Yunfeng Lei ◽  
Guodong Liu ◽  
Guangshan Yao ◽  
Zhonghai Li ◽  
Yuqi Qin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Responses ◽  
Penicillium Oxalicum ◽  
Bzip Transcription Factor ◽  
Multiple Stress ◽  
Cellulase Expression

scholarly journals Genome-Wide Identification and Expression Analyses of the bZIP Transcription Factor Genes in moso bamboo (Phyllostachys edulis)

2019 ◽  
Vol 20 (9) ◽  
pp. 2203 ◽  
Author(s):  
Feng Pan ◽  
Min Wu ◽  
Wenfang Hu ◽  
Rui Liu ◽  
Hanwei Yan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Responses ◽  
Transcriptional Activation ◽  
Growth And Development ◽  
Expression Profiles ◽  
Forest Products ◽  
Regulatory Elements ◽  
Moso Bamboo ◽  
Bzip Transcription Factor ◽  
Phyllostachys Edulis

The basic leucine zipper (bZIP) transcription factor (TF) family is one of the largest gene families, and play crucial roles in many processes, including stress responses, hormone effects. The TF family also participates in plant growth and development. However, limited information is available for these genes in moso bamboo (Phyllostachys edulis), one of the most important non-timber forest products in the world. In the present study, 154 putative PhebZIP genes were identified in the moso bamboo genome. The phylogenetic analyses indicate that the PhebZIP gene proteins classify into 9 subfamilies and the gene structures and conserved motifs that analyses identified among all PhebZIP proteins suggested a high group-specificity. Microsynteny and evolutionary patterns analyses of the non-synonymous (Ka) and synonymous (Ks) substitution rates and their ratios indicated that paralogous pairs of PhebZIP genes in moso bamboo underwent a large-scale genome duplication event that occurred 7–15 million years ago (MYA). According to promoter sequence analysis, we further selected 18 genes which contain the higher number of cis-regulatory elements for expression analysis. The result showed that these genes are extensively involved in GA-, ABA- and MeJA-responses, with possibly different mechanisms. The tissue-specific expression profiles of PhebZIP genes in five plant tissues/organs/developmental stages suggested that these genes are involved in moso bamboo organ development, especially seed development. Subcellular localization and transactivation activity analysis showed that PhebZIP47 and PhebZIP126 were localized in the nucleus and PhebZIP47 with no transcriptional activation in yeast. Our research provides a comprehensive understanding of PhebZIP genes and may aid in the selection of appropriate candidate genes for further cloning and functional analysis in moso bamboo growth and development, and improve their resistance to stress during their life.

scholarly journals The Basic Leucine Zipper Transcription Factor Moatf1 Mediates Oxidative Stress Responses and Is Necessary for Full Virulence of the Rice Blast Fungus Magnaporthe oryzae

2010 ◽  
Vol 23 (8) ◽  
pp. 1053-1068 ◽  
Author(s):  
Min Guo ◽  
Wang Guo ◽  
Yue Chen ◽  
Suomeng Dong ◽  
Xing Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Magnaporthe Oryzae ◽  
Stress Responses ◽  
Rice Blast ◽  
Leucine Zipper ◽  
Blast Disease ◽  
Bzip Transcription Factor ◽  
Wild Type ◽  
Basic Leucine Zipper

Magnaporthe oryzae is the causal agent of rice blast disease, leading to enormous losses of rice production. Here, we characterized a basic leucine zipper (bZIP) transcription factor, Moatf1, in M. oryzae, a homolog of Schizosaccharomyces pombe ATF/CREB that regulates the oxidative stress response. Moatf1 deletion caused retarded vegetative growth of mycelia, and the Moatf1 mutant exhibited higher sensitivity to hydrogen peroxide (H2O2) than did the wild-type strain. The mutant showed severely reduced activity of extracellular enzymes and transcription level of laccases and peroxidases and exhibited significantly reduced virulence on rice cultivar CO-39. On rice leaf sheath, most of the infectious hyphae of the mutant became swollen and displayed restricted growth in primary infected cells. Defense response was strongly activated in plants infected by the mutant. Diamino benzidine staining revealed an accumulation of H2O2 around Moatf1 mutant appressoria and rice cells with Moatf1 hyphae that was absent in the wild type. Inhibition of the plant NADPH oxidase by diphenyleneiodonium prevented host-derived H2O2 accumulation and restored infectious hyphal growth of the mutant in rice cells. Thus, we conclude that Moatf1 is necessary for full virulence of M. oryzae by regulating the transcription of laccases and peroxidases to impair reactive oxygen species–mediated plant defense.

scholarly journals A unique bZIP transcription factor imparting multiple stress tolerance in Rice

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Priyanka Das ◽  
Nita Lakra ◽  
Kamlesh Kant Nutan ◽  
Sneh Lata Singla-Pareek ◽  
Ashwani Pareek
Keyword(s):  
Transcription Factor ◽  
Stress Tolerance ◽  
Bzip Transcription Factor ◽  
Multiple Stress ◽  
Multiple Stress Tolerance

scholarly journals The bZIP Transcription Factor GmbZIP15 Negatively Regulates Salt- and Drought-Stress Responses in Soybean

2020 ◽  
Vol 21 (20) ◽  
pp. 7778
Author(s):  
Man Zhang ◽  
Yanhui Liu ◽  
Hanyang Cai ◽  
Mingliang Guo ◽  
Mengnan Chai ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Responses ◽  
Negative Regulator ◽  
Bzip Transcription Factor ◽  
Antioxidant Enzyme Activities ◽  
Oilseed Crop ◽  
Abiotic Stress Response ◽  
Salt And Drought Stress

Soybean (Glycine max), as an important oilseed crop, is constantly threatened by abiotic stress, including that caused by salinity and drought. bZIP transcription factors (TFs) are one of the largest TF families and have been shown to be associated with various environmental-stress tolerances among species; however, their function in abiotic-stress response in soybean remains poorly understood. Here, we characterized the roles of soybean transcription factor GmbZIP15 in response to abiotic stresses. The transcript level of GmbZIP15 was suppressed under salt- and drought-stress conditions. Overexpression of GmbZIP15 in soybean resulted in hypersensitivity to abiotic stress compared with wild-type (WT) plants, which was associated with lower transcript levels of stress-responsive genes involved in both abscisic acid (ABA)-dependent and ABA-independent pathways, defective stomatal aperture regulation, and reduced antioxidant enzyme activities. Furthermore, plants expressing a functional repressor form of GmbZIP15 exhibited drought-stress resistance similar to WT. RNA-seq and qRT-PCR analyses revealed that GmbZIP15 positively regulates GmSAHH1 expression and negatively regulates GmWRKY12 and GmABF1 expression in response to abiotic stress. Overall, these data indicate that GmbZIP15 functions as a negative regulator in response to salt and drought stresses.

scholarly journals Genome-Wide Identification of bZIP Transcription Factor Genes and Functional Analyses of Two Members in Cytospora chrysosperma

2021 ◽  
Vol 8 (1) ◽  
pp. 34
Author(s):  
Dasen Wen ◽  
Lu Yu ◽  
Dianguang Xiong ◽  
Chengming Tian
Keyword(s):  
Transcription Factor ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Pathogenic Fungus ◽  
Fungal Growth ◽  
Nutrient Utilization ◽  
Bzip Transcription Factor ◽  
Deletion Mutants ◽  
Fungal Virulence ◽  
Cytospora Chrysosperma

The basic leucine zipper (bZIP) transcription factor (TF) family, one of the largest and the most diverse TF families, is widely distributed across the eukaryotes. It has been described that the bZIP TFs play diverse roles in development, nutrient utilization, and various stress responses in fungi. However, little is known of the bZIP members in Cytospora chrysosperma, a notorious plant pathogenic fungus, which causes canker disease on over 80 woody plant species. In this study, 26 bZIP genes were systematically identified in the genome of C. chrysosperma, and two of them (named CcbZIP05 and CcbZIP23) significantly down-regulated in CcPmk1 deletion mutant (a pathogenicity-related mitogen-activated protein kinase) were selected for further analysis. Deletion of CcbZIP05 or CcbZIP23 displayed a dramatic reduction in fungal growth but showed increased hypha branching and resistance to cell wall inhibitors and abiotic stresses. The CcbZIP05 deletion mutants but not CcbZIP23 deletion mutants were more sensitive to the hydrogen peroxide compared to the wild-type and complemented strains. Additionally, the CcbZIP23 deletion mutants produced few pycnidia but more pigment. Remarkably, both CcbZIP05 and CcbZIP23 deletion mutants were significantly reduced in fungal virulence. Further analysis showed that CcbZIP05 and CcbZIP23 could regulate the expression of putative effector genes and chitin synthesis-related genes. Taken together, our results suggest that CcbZIP05 and CcbZIP23 play important roles in fungal growth, abiotic stresses response, and pathogenicity, which will provide comprehensive information on the CcbZIP genes and lay the foundation for further research on the bZIP members in C. chrysosperma.

The bHLH transcription factor ILR3 modulates multiple stress responses in Arabidopsis

2018 ◽  
Vol 97 (4-5) ◽  
pp. 297-309 ◽  
Author(s):  
Rozalynne Samira ◽  
Baohua Li ◽  
Daniel Kliebenstein ◽  
Chunying Li ◽  
Eric Davis ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Responses ◽  
Bhlh Transcription Factor ◽  
Multiple Stress

scholarly journals A Homeodomain-Containing Transcriptional Factor PoHtf1 Regulated the Development and Cellulase Expression in Penicillium oxalicum

2021 ◽  
Vol 12 ◽  
Author(s):  
Hao Guo ◽  
Gen Xu ◽  
Ruimei Wu ◽  
Zhigang Li ◽  
Mengdi Yan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Regulatory Network ◽  
Colony Growth ◽  
Penicillium Oxalicum ◽  
Yellow Pigment ◽  
Transcriptional Factor ◽  
Developmental Processes ◽  
Transcriptional Regulatory ◽  
Cellulase Expression ◽  
Biochemical Production

Homeodomain-containing transcription factors (Htfs) play important roles in animals, fungi, and plants during some developmental processes. Here, a homeodomain-containing transcription factor PoHtf1 was functionally characterized in the cellulase-producing fungi Penicillium oxalicum 114-2. PoHtf1 was shown to participate in colony growth and conidiation through regulating the expression of its downstream transcription factor BrlA, the key regulator of conidiation in P. oxalicum 114-2. Additionally, PoHtf1 inhibited the expression of the major cellulase genes by coordinated regulation of cellulolytic regulators CreA, AmyR, ClrB, and XlnR. Furthermore, transcriptome analysis showed that PoHtf1 participated in the secondary metabolism including the pathway synthesizing conidial yellow pigment. These data show that PoHtf1 mediates the complex transcriptional-regulatory network cascade between developmental processes and cellulolytic gene expression in P. oxalicum 114-2. Our results should assist the development of strategies for the metabolic engineering of mutants for applications in the enzymatic hydrolysis for biochemical production.

scholarly journals A novel family of dehydrin-like proteins is involved in stress response in the human fungal pathogenAspergillus fumigatus

2011 ◽  
Vol 22 (11) ◽  
pp. 1896-1906 ◽  
Author(s):  
Joanne Wong Sak Hoi ◽  
Claude Lamarre ◽  
Rémi Beau ◽  
Isabelle Meneau ◽  
Adokiye Berepiki ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
Stress Responses ◽  
Fluorescent Protein ◽  
Mapk Pathway ◽  
Experimental Models ◽  
Mitogen Activated Protein Kinase ◽  
Bzip Transcription Factor ◽  
Mitogen Activated Protein ◽  
Protective Proteins

 During a search for genes controlling conidial dormancy in Aspergillus fumigatus, two dehydrin-like genes, DprA and DprB, were identified. The deduced proteins had repeated stretches of 23 amino acids that contained a conserved dehydrin-like protein (DPR) motif. Disrupted DprAΔ mutants were hypersensitive to oxidative stress and to phagocytic killing, whereas DprBΔ mutants were impaired in osmotic and pH stress responses. However, no effect was observed on their pathogenicity in our experimental models of invasive aspergillosis. Molecular dissection of the signaling pathways acting upstream showed that expression of DprA was dependent on the stress-activated kinase SakA and the cyclic AMP-protein kinase A (cAMP-PKA) pathways, which activate the bZIP transcription factor AtfA, while expression of DprB was dependent on the SakA mitogen-activated protein kinase (MAPK) pathway, and the zinc finger transcription factor PacC. Fluorescent protein fusions showed that both proteins were associated with peroxisomes and the cytosol. Accordingly, DprA and DprB were important for peroxisome function. Our findings reveal a novel family of stress-protective proteins in A. fumigatus and, potentially, in filamentous ascomycetes.

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