Influence of exogenous cinnamic acid on the production of chlorogenic acid in Cichorium intybus L cell culture

The extractable activities of thirteen enzymes of primary and secondary metabolism have been measured in chickpea (Cicer arietinum L.) cell suspension cultures after treatment with an elicitor from the fungus Ascochyta rabiei (Pass.) Lab. The cell culture, derived from the A. rabiei resistant cultivar ILC 3279, constitutively accumulated the isoflavones biochanin A and formononetin together with their 7-O-glucosides and the 7-O-glucoside-6″-malonates. After elicitor application the cells rapidly form the pterocarpan phytoalexins medicarpin and maackiain. Among the enzymes of primary metabolism only the glucose 6-phosphate dehydrogenase exhibited a significant increase in activity with a maximum four hours after application of the elicitor. In phenylpropane metabolism the activities of phenylalanine ammonia lyase and chalcone synthase were enhanced by the elicitor and exhibited highest levels after four hours. In contrast the chalcone isomerase activity was not influenced by the elicitor. A substantial enhancement occurred with the isoflavone 7-O-glucosyltransferase activity eight hours after elicitor application. The results suggest that in this cell culture the elicitor-induced biosynthesis of pterocarpan phytoalexins was accompanied with a rapid and transient increase of those enzyme activities which are located at branching points of related pathways, i.e. pentose phosphate cycle, general phenylpropane metabolism, flavonoid formation and isoflavone conjugation.

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Determination of cinnamic acid and 4-coumaric acid in alfalfa (Medicago sativa L.) cell suspension cultures by gas chromatography

Phytochemical Analysis ◽

10.1002/pca.2800040309 ◽

1993 ◽

Vol 4 (3) ◽

pp. 124-130 ◽

Cited By ~ 4

Author(s):

John D. Orr ◽

Lloyd W. Sumner ◽

Robert Edwards ◽

Richard A. Dixon

Keyword(s):

Gas Chromatography ◽

Medicago Sativa ◽

Cell Suspension ◽

Cinnamic Acid ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Coumaric Acid ◽

L Cell ◽

Medicago Sativa L

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Analysis of triterpenoids, carotenoids, and phenylpropanoids in the flowers, leaves, roots, and stems of white bitter melon (Cucurbitaceae, Momordica charantia)

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v20i1.22 ◽

2021 ◽

Vol 20 (1) ◽

pp. 155-160

Author(s):

Do Manh Cuong ◽

Ramaraj Sathasivam ◽

Chang Ha Park ◽

Hyeon Ji Yeo ◽

Ye Eun Park ◽

...

Keyword(s):

Chlorogenic Acid ◽

Gallic Acid ◽

Cinnamic Acid ◽

Developmental Stages ◽

Momordica Charantia ◽

Bitter Melon ◽

Coumaric Acid ◽

Direct Sunlight ◽

Β Carotene ◽

Different Parts

Purpose: To evaluate the contents of carotenoids, triterpenoids, and phenylpropanoids in different parts of white bitter melon.Methods: We evaluated the accumulation of 2 triterpenoids, 10 carotenoids, and 11 phenylpropanoids in different parts of white bitter melon, including fruits at four different developmental stages using HPLC.Results: Charantin, lutein, and rutin were the main triterpenoids, carotenoids, and phenylpropanoids, respectively. The accumulation of triterpenoids (momordicine and charantin), carotenoids (antheraxanthin, lutein, violaxanthin, α-carotene, and β-carotene), and phenylpropanoids (caffeic acid, chlorogenic acid, epicatechin, gallic acid, p-coumaric acid, rutin, and trans-cinnamic acid) was high inthe leaves and/or flowers, which are exposed to direct sunlight, but low in the roots.Conclusion: Most of the analyzed components were accumulated at high levels in the leaves and/or flowers. These results will help exploit the compounds in various parts of white bitter melon that are beneficial for human health. Keywords: Momordica charantia, Bitter melon, Triterpenoid, Carotenoid, Phenylpropanoid

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