scholarly journals Crude Moringa oleifera Lam. seed extract attenuates non-alcoholic fatty liver disease in growing Sprague–Dawley rats

2020 ◽  
Vol 129 ◽  
pp. 191-197
Author(s):  
M. Mapfumo ◽  
B.W. Lembede ◽  
P. Nkomozepi ◽  
A.R. Ndhlala ◽  
E. Chivandi
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Moringa Oleifera ◽  
Seed Extract ◽  
Sprague Dawley ◽  
Alcoholic Fatty Liver ◽  
Sprague Dawley Rats ◽  
Alcoholic Fatty Liver Disease ◽  
Moringa Oleifera Lam

scholarly journals The Effect of Rat Strain, Diet Composition and Feeding Period on the Development of a Nutritional Model of Non-Alcoholic Fatty Liver Disease in Rats

2011 ◽  
pp. 317-328 ◽  
Author(s):  
O. KUČERA ◽  
T. GARNOL ◽  
H. LOTKOVÁ ◽  
P. STAŇKOVÁ ◽  
Y. MAZUROVÁ ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Wistar Rats ◽  
Male Rats ◽  
Standard Diet ◽  
Sprague Dawley ◽  
Alcoholic Fatty Liver ◽  
Sprague Dawley Rats ◽  
Alcoholic Fatty Liver Disease

Non-alcoholic fatty liver disease (NAFLD) is an important cause of liver-related morbidity and mortality. The aim of this work was to establish and characterize a nutritional model of NAFLD in rats. Wistar or Sprague-Dawley male rats were fed ad libitum a standard diet (ST-1, 10 % kcal fat), a medium-fat gelled diet (MFGD, 35 % kcal fat) and a high-fat gelled diet (HFGD, 71 % kcal fat) for 3 or 6 weeks. We examined the serum biochemistry, the hepatic malondialdehyde, reduced glutathione (GSH) and cytokine concentration, the respiration of liver mitochondria, the expression of uncoupling protein-2 (UCP-2) mRNA in the liver and histopathological samples. Feeding with MFGD and HFGD in Wistar rats or HFGD in Sprague-Dawley rats induced small-droplet or mixed steatosis without focal inflammation or necrosis. Compared to the standard diet, there were no significant differences in serum biochemical parameters, except lower concentrations of triacylglycerols in HFGD and MFGD groups. Liver GSH was decreased in rats fed HFGD for 3 weeks in comparison with ST-1. Higher hepatic malondialdehyde was found in both strains of rats fed HFGD for 6 weeks and in Sprague-Dawley groups using MFGD or HFGD for 3 weeks vs. the standard diet. Expression of UCP-2 mRNA was increased in Wistar rats fed MFGD and HFGD for 6 weeks and in Sprague-Dawley rats using HFGD for 6 weeks compared to ST-1. The present study showed that male Wistar and Sprague-Dawley rats fed by HFGD developed comparable simple steatosis without signs of progression to non-alcoholic steatohepatitis under our experimental conditions.

The effect of single clove Black garlic on the hemostasis status and lipid profile in male Sprague Dawley rats with non-alcoholic fatty liver disease

10.5219/1516 ◽  
2021 ◽  
Vol 15 ◽  
pp. 387-395
Author(s):  
Lili Nurmawati ◽  
Mohammad Sulchan ◽  
Siti Fatimah-Muis ◽  
Hery Djagat Purnomo ◽  
Kis Djamiatun ◽  
...  
Keyword(s):  
Liver Disease ◽  
Treatment Group ◽  
Fatty Liver Disease ◽  
Lipid Profiles ◽  
Control Group ◽  
Sprague Dawley ◽  
Alcoholic Fatty Liver ◽  
Sprague Dawley Rats ◽  
Alcoholic Fatty Liver Disease ◽  
Pai 1

Non-alcoholic fatty liver disease (NAFLD) patients have alteration in hemostasis components. Thus, including excess expression of plasminogen activator inhibitor-1 (PAI-1), causing fibrinolysis disorders; the majority of these patients are hypercoagulable state prone to thrombosis. Some evidence suggests that garlic and garlic supplements have antithrombotic and anti-inflammatory properties. Besides, garlic stimulates fibrinolytic activity and normalizes plasma lipid imbalances. Black garlic is processed garlic that is produced through natural aging at a controlled temperature (70 °C) and high humidity (90%) for several days, without other additives. This study aimed to prove the effect of single clove black garlic (SCBG) (Allium sativum Linn) on PAI-1 levels and lipid profiles of NAFLD rats induced by a high-fat fructose diet (HFFD) containing 1.25% cholesterol and 0.5% cholic acid.The rats were then divided into healthy control group (K1+); NAFLD control group without treatment (K2+); 0.9 mg simvastatin treatment group (K1-); 45 mg metformin treatment group (K2-); SCBG 450 mg per 200g BW (X1); 900 mg per 200 g BW (X2); and 1350 mg per 200 g BW (X3). All treatments were administered for 4 weeks via oral gavage. As a result, significant differences in PAI-1 levels and lipid profiles between groups after the administration (p = 0.001) were noted and also by simvastatin and metformin, respectively. There was a correlation between PAI-1 and lipid profile of SCBG treatment. In conclusion, the administration of SCBG (1350 mg per 200 g BB per day) for 4 weeks had a significant effect on PAI-1 levels, and the lipid profiles in Sprague Dawley rats modeled NAFLD (p = 0.001). SCBG has provided benefits that can be useful in the management of NAFLD but it’s not equivalent to medicine.

Histological Effects of Aqueous Extract of Moringa oleifera on the Liver Tissues of Wistar rats fed with High Fat Diet

2017 ◽  
Vol 2 (2) ◽  
pp. 1
Author(s):  
Obayuwana E, Obayuwana E, Ubehenin A.E, Innih S.O, Iwee R, Eidaghese E.C
Keyword(s):  
Body Weight ◽  
Liver Disease ◽  
Fatty Liver ◽  
Aqueous Extract ◽  
High Fat Diet ◽  
Fatty Liver Disease ◽  
Moringa Oleifera ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

Moringa oleifera belongs to family of moringaceae and is considered as one of the world’s most useful trees, as almost every part of the plant can be used for either as food, or therapeutic purposes. Fatty diet is a significant factor in the pathogenesis of non-alcoholic fatty Liver disease (NAFLD). The study was designed to examine histological effects of aqueous extract of Moringa oleifera on the liver tissues of wistar rat fed with high fat diet. Twenty five adult rats were divided into five groups of five animals each .While group A received distilled water daily only, groups Band C received aqueous extract of Moringa oleifera at doses of 200 mg/kg body weight and fat high diet (30% w/w of the total mash feed) respectively for a duration of seventy days. Others (groups D and E) received aqueous extract of Moringa oleifera at doses of 200 mg/kg body weight and fat high diet for a duration of seventy days. Histology of the liver of the rats fed with high fat diet exhibited significant changes in the architecture of liver tissue. The changes include micro and macro vascular steatosis, increased fatty infiltration, inflammation, sinusoidal dilation, degeneration of veins and vacuolization as compared to normal liver histology. Treatment with 200 mg/kg extract of Moringa oleifera significantly attenuated these effects imposed by high fat diet as compared to the control group. Therefore demonstrated that daily administration of Moringa oleifera leaves extract to rats for a period of 70 days may reverse the formation of hepatic steatosis in non-alcoholic fatty liver disease.

scholarly journals Integrating Network Pharmacology and RT-qPCR Analysis to Investigate the Mechanisms Underlying ZeXie Decoction-Mediated Treatment of Non-alcoholic Fatty Liver Disease

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiashuo Wu ◽  
Fangqing Zhang ◽  
Haonan Ruan ◽  
Xiaoyan Chang ◽  
Jingxun Wang ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Network Pharmacology ◽  
Sprague Dawley ◽  
Alcoholic Fatty Liver ◽  
Pathway Network ◽  
Experimental Pharmacology ◽  
Mechanistic Basis ◽  
Alcoholic Fatty Liver Disease

ZeXie Decoction (ZXD) is a traditional Chinese medicine composed of Alisma orientalis (Sam.) Juzep. and Atractylodes macrocephala Koidz. ZXD has been widely used to treat non-alcoholic fatty liver disease (NAFLD). The mechanistic basis for the pharmacological activity of ZXD, however, remains poorly understood. In this study, we used a network pharmacology approach and investigated the association between ZXD and NAFLD. We identified the active ingredients of ZXD and screened the potential targets of these ingredients, after which a database of relevant NAFLD-related targets were constructed and several enrichment analyses were performed. Furthermore, the ethanol and aqueous extracts of ZXD were prepared and experimental pharmacology validation was conducted using RT-qPCR of the non-alcoholic fatty liver disease (NAFLD) model in Sprague-Dawley (SD) rats. As a result, a herb-compound-target-pathway network model was developed, and HMGCR, SREBP-2, MAPK1, and NF-κBp65 targets were validated. The gene expression results of these four targets were consistent with those of the network pharmacology prediction. Using an integration strategy, we revealed that ZXD could treat NAFLD by targeting HMGCR, SREBP-2, MAPK1, and NF-κBp65.

scholarly journals Pemanfaatan Tanaman Kelor (Moringa oleifera Lam.) sebagai Sumber Terapi Preventif dan Kuratif pada Pasien Perlemakan Hepar dengan Sindrom Metabolik

2020 ◽  
Vol 1 (2) ◽  
pp. 12
Author(s):  
Tiara Mahza Wardhani
Keyword(s):  
Metabolic Syndrome ◽  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Moringa Oleifera ◽  
International Diabetes Federation ◽  
Alcoholic Fatty Liver ◽  
Curative Therapy ◽  
Tnf Α ◽  
Moringa Oleifera Lam

ABSTRAK Menurut The International Diabetes Federation (IDF) pada tahun 2015, sindrom metabolik ditemukan pada 25% populasi orang dewasa dunia dan diperkirakan akan terus meningkat. Sindrom metabolik menyebabkan peningkatan produk metabolik seperti trigliserida dan tetesan lemak hepatik yang berujung pada perlemakan hati. Tinjauan  pustaka ini bertujuan untuk mengetahui efek Moringa oleifera, Lam. sebagai sumber terapi preventif dan kuratif pada pasien perlemakan hati dengan sindrom metabolik. Tinjuan pustaka ini ditulis berdasarkan artikel yang dikumpulkan melalui beberapa mesin pencari ilmiah dengan kriteria inklusi dan ekslusi. Hasil studi didasarkan literatur yang telah teruji validitasnya dan  relevan dengan kajian analisis pembahasan. Setelah data yang diperlukan terkumpul, dilakukan pengolahan data dengan menyusun secara sistematis dan logis. Moringa oleifera, Lam.  menginhibisi TNF-α.,IL-1β, IL-6, dan IL-10 sehingga bersifat hepatoprotektif. Selain itu,  Moringa oleifera, Lam. menghambat lipogenesis melalui jalur Nf-kb yang ditandai turunnya LDL-R, SRB1c, DGAT2, PPARy dan peningkatan sensitivitas sekresi insulin sehingga menghambat  perlemakan hati. Moringa oleifera, Lam. Dapat digunakan sebagai sumber terapi preventif dan kuratif pada pasien perlemakan hati dengan sindrom metabolik. Kata Kunci: Moringa oleifera, Kelor, Trigliserida, Perlemakan hati, Sindrom Metabolik   ABSTRACT Based on The International Diabetes Federation research in 2015, metabolic syndrome found in 25% of the adult population in the world. Metabolic syndrome increases metabolic end products like triglyceride and steatosis which is causing non-alcoholic fatty liver.  This literature review aimed to determine the effect of Moringa oleifera, Lam.  as the source of preventive and curative therapy in the non-alcoholic fatty liver with metabolic syndrome. This literature review is based on journals and articles collected from search engines with some inclusion and exclusion criteria. Only valid and relevant works of literature consider as the preference. Moringa oleifera, Lam.  has hepatoprotective effect by inhibits TNF-α., IL-1β, IL-6, dan IL-10. Moringa oleifera, Lam. also inhibits fatty liver disease by inhibits lipogenesis through the Nf-kb pathway marked by decreasing LDL-R, SRB1c, DGAT2, PPARy, and increasing insulin sensitivity. Moringa oleifera, Lam. is useful for the natural source of preventive and curative therapy in the non-alcoholic fatty liver with metabolic syndrome. Keyword: Moringa oleifera, Triglyceride, Fatty Liver Disease, Metabolic Syndrome

scholarly journals Hepatoprotective Effect of Ajwa Date Seed and Simvastatin in Non Alcoholic Fatty Liver Disease (NAFLD) Murine Model of Butter Fed Dyslipidemia

2019 ◽  
Vol 33 (4) ◽  
pp. 1-6
Author(s):  
Farooq Sultan
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Seed Extract ◽  
Control Group ◽  
Alcoholic Fatty Liver ◽  
Date Seed ◽  
Hepatoprotective Effects ◽  
Animal House ◽  
Alcoholic Fatty Liver Disease

Introduction: Non-alcoholic fatty liver disease (NAFLD) is a dangerous complication of hyperlipidemia which may lead to life threatening hepatitis and hepatic cirrhosis. Internationally, Prevalence of non-alcoholic fatty liver disease (NAFLD) is 20% in general population while 75% in the obese population of USA.1 Side effects of allopathic drugs have led the research towards natural products to find out their potential for treatment of NAFLD. Aims & Objectives: Aim of this study was evaluation of hepatoprotective effects of Ajwa date seed and Simvastatin against NAFLD. Place and duration of study: Study was conducted at animal house of UHS, Lahore and completed in 6 months. Material & Methods: 40 rats were procured and segregated into 5 groups with 8 rats in each group. Group 1 was healthy control and group 2 was disease control. Group 3, 4 and 5 were administered Simvastatin 10mg/kg/day, aqueous Ajwa date seed extract (ADSE) 8ml/kg/day and combination of Simvastatin 5mg/kg/day and aqueous ADSE 4ml/kg/day respectively. Total study duration was 36 days. First 15 days were for induction of hyperlipidemia while treatment was given for remaining 21 days. Serum total cholesterol (TC), triglycerides (TG) and liver functions tests (Serum ALT, AST and ALP) were performed at day 0, 15 and 36 of study. Data was entered on SPSS 23.0 software and analyzed by one way ANOVA and post hoc tuckey’s tests. P-value of less than 0.05 was considered significant. Results: Aqueous Ajwa date seed extract (ADSE), Simvastatin and their combination showed significant TC and TG lowering and hepatoprotective effects against NAFLD. However, individual drugs were more effective than the combination. Conclusion: Ajwa date seed can be used for the treatment of NAFLD. Key words: Ajwa date seed, Hyperlipidemia, Simvastatin

Sign in / Sign up

Export Citation Format

Share Document