The effect of IBA on anatomical changes and antioxidant enzyme activity during the in vitro rooting of smoke tree ( Cotinus coggygria Scop.)

Flavonoids obtained from Enhydra fluctuans (FEF) were screened for anticancer activity against Ehrlich's ascites carcinoma (EAC) bearing Swiss albino mice. The anticancer activity was assessed by measuring the tumor growth response, percentage increase of life span, hematological parameters, lipid peroxidation, and antioxidant enzyme activity, like GSH and CAT. Two flavonoids, baicalein 7- O-glucoside and baicalein 7- O-diglucoside, were isolated from the ethyl acetate fraction. Treatment with FEF caused a significant decrease in the tumor cell volume and increase of life span. All the hematological parameters, malonaldehyde content and antioxidant enzyme activity were restored towards the normal level. FEF was found to be cytotoxic in the in-vitro model.

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Antioxidant Properties of White Sesame (Sesamum indicum L.) Flour on Human Liver Cells In Vitro

Proceedings ◽

10.3390/foods_2020-07620 ◽

2020 ◽

Vol 70 (1) ◽

pp. 39

Author(s):

Maria Eugenia Sabatino ◽

Agustín Lucini Mas ◽

Martin Gustavo Theumer ◽

Marcela Martinez ◽

Daniel Wundelin ◽

...

Keyword(s):

Enzyme Activity ◽

Antioxidant Enzyme ◽

Antioxidant Properties ◽

Sesamum Indicum ◽

Antioxidant Enzyme Activity ◽

Beneficial Effects ◽

Potential Source ◽

Oxidative Effects ◽

Gpx Activity

White sesame (Sesamum indicum L.) flour, an edible residue resulting from seed oil extraction, may become a novel alternative of healthy products due to its high polyphenols content—compounds with many beneficial effects for human health. In the present study, the antioxidant properties of sesame flour were characterized in human cells in vitro. The hepatic cell line HepG2 was treated for 24 h with degreased sesame flour extracts or pinoresinol—one of the main polyphenols of this flour. Then oxidative stress was induced by H2O2 exposure. Cell viability and reactive oxygen species amount were measured by flow cytometry. Antioxidant enzyme activity of glutathione peroxidase (GPx), reductase (GR) and catalase (CAT) were determined by spectrophotometry. Results showed that pinoresinol decreased H2O2 oxidative effects by up to a 40%, increasing CAT and GR activity without compromising cellular viability. The largest dose of sesame flour extracts decreased H2O2 stress induction by up to 46%, but increased cell death levels. Additionally, sesame flour raised CAT and GPx activity by 100%. These findings suggest that sesame flour has antioxidant properties through antioxidant enzyme activity modulation. Since pinoresinol is one of the major polyphenols of sesame flour, this industrial residue might be a potential source for functional foods with health benefits.

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Interactive effects of aluminum and cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry ( Vaccinium corymbosum L.) plantlets cultivated in vitro

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2017.12.050 ◽

2018 ◽

Vol 150 ◽

pp. 320-326 ◽

Cited By ~ 22

Author(s):

K. Manquián-Cerda ◽

E. Cruces ◽

M. Escudey ◽

G. Zúñiga ◽

R. Calderón

Keyword(s):

Oxidative Stress ◽

Enzyme Activity ◽

Phenolic Compounds ◽

Antioxidant Enzyme ◽

Vaccinium Corymbosum ◽

Antioxidant Enzyme Activity ◽

Interactive Effects ◽

And Oxidative Stress

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Hormonal and Local Factors Influence Antioxidant Enzyme Activity of Rat Fetal Lung Cells in Vitro

Experimental Lung Research ◽

10.3109/01902148609062826 ◽

1986 ◽

Vol 11 (1) ◽

pp. 49-59 ◽

Cited By ~ 21

Author(s):

A. K. Tanswell ◽

M. G. Tzaki ◽

P. J. Byrne

Keyword(s):

Enzyme Activity ◽

Antioxidant Enzyme ◽

Fetal Lung ◽

Antioxidant Enzyme Activity ◽

Lung Cells ◽

Local Factors

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Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y2012-028 ◽

2012 ◽

Vol 90 (8) ◽

pp. 1095-1103 ◽

Cited By ~ 10

Author(s):

Martine Kienzle Hagen ◽

Ana Ludke ◽

Alex Sander Araujo ◽

Roberta Hack Mendes ◽

Tânia Gatelli Fernandes ◽

...

Keyword(s):

Oxidative Stress ◽

Enzyme Activity ◽

Antioxidant Enzyme ◽

Disease Model ◽

Fold Increase ◽

Antioxidant Enzyme Activity ◽

Nitrate Content ◽

Isolated Soy Protein ◽

Nitrite Nitrate

This study analyzed and compared the content of isoflavones in 2 soy products, the effectiveness of isoflavones as antioxidants, in vitro, and demonstrated the antioxidant effect of a soy diet in rats with myocardial infarction (MI). Isoflavone content was analyzed in soybean hypocotyl (SH) and isolated soy protein (ISP). The quality (TAR) and quantity (TRAP) of antioxidants present in the samples was quantified. The amount of daidzin was higher in SH (9 times) and genistein in ISP (5 times). SH presented a 3-fold increase in TAR, while both products exhibited same TRAP. The rats were fed an ISP diet for 9 weeks. Animals were distributed among 6 treatment groups: (i) Sham Casein; (ii) Infarct Casein < 25%; (iii) Infarct Casein > 25%; (iv) Sham Soy; (v) Infarct Soy < 25%; and (vi) Infarct Soy > 25%. MI was induced 5 weeks after the commencement of the diets. Lipid peroxidation (LPO), antioxidant enzyme activity, and levels of nitrites/nitrates were determined in blood. Rats receiving the ISP diet demonstrated increased activity of antioxidant enzyme activity and nitrite/nitrate content. In addition, the increase in LPO seen in rats subjected to MI was significantly mitigated when the ISP diet was given. These findings suggest a nutritional approach of using a soy-based diet for the prevention of oxidative-stress-related diseases such as heart failure.

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