scholarly journals Antioxidant Properties of White Sesame (Sesamum indicum L.) Flour on Human Liver Cells In Vitro

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 39
Author(s):  
Maria Eugenia Sabatino ◽  
Agustín Lucini Mas ◽  
Martin Gustavo Theumer ◽  
Marcela Martinez ◽  
Daniel Wundelin ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Antioxidant Properties ◽  
Sesamum Indicum ◽  
Antioxidant Enzyme Activity ◽  
Beneficial Effects ◽  
Potential Source ◽  
Oxidative Effects ◽  
Gpx Activity

White sesame (Sesamum indicum L.) flour, an edible residue resulting from seed oil extraction, may become a novel alternative of healthy products due to its high polyphenols content—compounds with many beneficial effects for human health. In the present study, the antioxidant properties of sesame flour were characterized in human cells in vitro. The hepatic cell line HepG2 was treated for 24 h with degreased sesame flour extracts or pinoresinol—one of the main polyphenols of this flour. Then oxidative stress was induced by H2O2 exposure. Cell viability and reactive oxygen species amount were measured by flow cytometry. Antioxidant enzyme activity of glutathione peroxidase (GPx), reductase (GR) and catalase (CAT) were determined by spectrophotometry. Results showed that pinoresinol decreased H2O2 oxidative effects by up to a 40%, increasing CAT and GR activity without compromising cellular viability. The largest dose of sesame flour extracts decreased H2O2 stress induction by up to 46%, but increased cell death levels. Additionally, sesame flour raised CAT and GPx activity by 100%. These findings suggest that sesame flour has antioxidant properties through antioxidant enzyme activity modulation. Since pinoresinol is one of the major polyphenols of sesame flour, this industrial residue might be a potential source for functional foods with health benefits.

Heat stress and antioxidant enzyme activity in bubaline (Bubalus bubalis) oocytes during in vitro maturation

2016 ◽  
Vol 60 (9) ◽  
pp. 1357-1366 ◽  
Author(s):  
Syma Ashraf Waiz ◽  
Mohammad Raies-ul-Haq ◽  
Suman Dhanda ◽  
Anil Kumar ◽  
T. Sridhar Goud ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Heat Stress ◽  
Antioxidant Enzyme ◽  
In Vitro Maturation ◽  
Bubalus Bubalis ◽  
Antioxidant Enzyme Activity

scholarly journals Effect of Pulsed Ultraviolet Light on Natural Microbial Load and Antioxidant Properties of Fresh Blueberries

2018 ◽  
Vol 6 (6) ◽  
pp. 733
Author(s):  
Senem Guner ◽  
Zeynal Topalcengiz
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Properties ◽  
Antioxidant Enzyme Activity ◽  
Microbial Load ◽  
Plate Count ◽  
Light Illumination ◽  
Sod Activity ◽  
Aerobic Plate Count

Blueberries have been drawn more attention in recent years for their antioxidant capacities and proposed health benefits. Pulsed ultraviolet (PUV) light is known for its disinfection effects on the surface of fresh fruits. This study’s aim was to examine the effects of PUV light on naturally present microbial load, antioxidant capacity, and antioxidant enzyme activity of fresh blueberries. Fresh blueberry samples were treated for 30, 60, 90, and 120 s. Samples were stabilized at 13 cm away from the PUV lamp before treatment. Total Aerobic Plate Count (TAPC) and yeast and mold count reduction were observed the highest in samples treated for 90 s, 1.97 and 1.27 log CFU/g, respectively. Antioxidant capacity levels were significantly different for treated samples for 60 and 90 s in FRAP analysis, whereas no differences were observed in results of DPPH analysis. Superoxide dismutase (SOD) activity as antioxidant enzyme had no difference, however, glutathione peroxidase (GPx) increased by 50% with PUV treatment for 120 s. PUV light treatment of fresh blueberries for 90 s was determined as ideal exposure time among tested time intervals of this study. PUV light illumination can generally enhance antioxidant capacity and antioxidant enzyme activity of fresh blueberries and decrease the population of natural microorganisms of fresh blueberries and similar small fruits.

scholarly journals Flavonoids of Enhydra Fluctuans Exhibit Anticancer Activity against Ehrlich's Ascites Carcinoma in Mice

2010 ◽  
Vol 5 (8) ◽  
pp. 1934578X1000500
Author(s):  
Santanu Sannigrahi ◽  
Upal Kanti Mazumder ◽  
Arijit Mondal ◽  
Dilipkumar Pal ◽  
Silpi Lipsa Mishra ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Life Span ◽  
Anticancer Activity ◽  
Antioxidant Enzyme ◽  
Hematological Parameters ◽  
Antioxidant Enzyme Activity ◽  
Percentage Increase ◽  
Ehrlich's Ascites Carcinoma ◽  
Ehrlich’S Ascites Carcinoma

Flavonoids obtained from Enhydra fluctuans (FEF) were screened for anticancer activity against Ehrlich's ascites carcinoma (EAC) bearing Swiss albino mice. The anticancer activity was assessed by measuring the tumor growth response, percentage increase of life span, hematological parameters, lipid peroxidation, and antioxidant enzyme activity, like GSH and CAT. Two flavonoids, baicalein 7- O-glucoside and baicalein 7- O-diglucoside, were isolated from the ethyl acetate fraction. Treatment with FEF caused a significant decrease in the tumor cell volume and increase of life span. All the hematological parameters, malonaldehyde content and antioxidant enzyme activity were restored towards the normal level. FEF was found to be cytotoxic in the in-vitro model.

PO2-dexamethasone interactions in fibroblast growth and antioxidant enzyme activity

1987 ◽  
Vol 252 (4) ◽  
pp. C396-C400 ◽  
Author(s):  
P. S. Randhawa ◽  
M. A. Hass ◽  
L. Frank ◽  
D. Massaro
Keyword(s):  
Enzyme Activity ◽  
Protein Content ◽  
Antioxidant Enzyme ◽  
Fetal Lung ◽  
Antioxidant Enzyme Activity ◽  
Lung Fibroblasts ◽  
Cell Multiplication ◽  
Sod Activity ◽  
Lower Protein ◽  
Gpx Activity

Fetal rat lung fibroblasts were cultured in a gas phase of 20% O2, 5% CO2 (PO2 measured, 150 Torr) or 2% oxygen, 5% CO2 (PO2 measured, 25 Torr) with or without 100 nM dexamethasone (Dex). Superoxide dismutase (SOD) activity per cell increased spontaneously during 4 days of incubation at both PO2, but catalase (CAT) activity tended to fall during this time and glutathione peroxidase (GPx) activity showed no consistent trend during this interval. Cells cultured at a low PO2 had a lower protein content and SOD activity compared with air controls. Dex inhibited cell proliferation and enhanced intracellular accumulation of protein at the low PO2 but prevented the increase in protein content without affecting cell multiplication at a PO2 of 150 Torr. SOD activity per cell was enhanced by Dex at a low PO2 but reduced in 20% O2, 5% CO2. An increase in CAT and GPx activity per cell resulted on exposing fibroblasts to Dex in the presence of low PO2. These results show that Dex affects the growth and antioxidant enzyme activity of fetal lung fibroblasts, and this action of Dex can be modulated by changing the ambient PO2.

Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model

2012 ◽  
Vol 90 (8) ◽  
pp. 1095-1103 ◽  
Author(s):  
Martine Kienzle Hagen ◽  
Ana Ludke ◽  
Alex Sander Araujo ◽  
Roberta Hack Mendes ◽  
Tânia Gatelli Fernandes ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Disease Model ◽  
Fold Increase ◽  
Antioxidant Enzyme Activity ◽  
Nitrate Content ◽  
Isolated Soy Protein ◽  
Nitrite Nitrate

This study analyzed and compared the content of isoflavones in 2 soy products, the effectiveness of isoflavones as antioxidants, in vitro, and demonstrated the antioxidant effect of a soy diet in rats with myocardial infarction (MI). Isoflavone content was analyzed in soybean hypocotyl (SH) and isolated soy protein (ISP). The quality (TAR) and quantity (TRAP) of antioxidants present in the samples was quantified. The amount of daidzin was higher in SH (9 times) and genistein in ISP (5 times). SH presented a 3-fold increase in TAR, while both products exhibited same TRAP. The rats were fed an ISP diet for 9 weeks. Animals were distributed among 6 treatment groups: (i) Sham Casein; (ii) Infarct Casein < 25%; (iii) Infarct Casein > 25%; (iv) Sham Soy; (v) Infarct Soy < 25%; and (vi) Infarct Soy > 25%. MI was induced 5 weeks after the commencement of the diets. Lipid peroxidation (LPO), antioxidant enzyme activity, and levels of nitrites/nitrates were determined in blood. Rats receiving the ISP diet demonstrated increased activity of antioxidant enzyme activity and nitrite/nitrate content. In addition, the increase in LPO seen in rats subjected to MI was significantly mitigated when the ISP diet was given. These findings suggest a nutritional approach of using a soy-based diet for the prevention of oxidative-stress-related diseases such as heart failure.

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